Rw7G014620

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004720
Physical Location & Seq
Forward (+)
14326302 .. 14340508
14207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014620.1

Sequence Viewer

Length: 5556 bp
ATGGGTAAGGGAAGACCTAGGGCTGTGGAGAAGGGGGTGGTGGGGCCCAATTTGAGTGCTTCATCGTCTTCTTCGTCTGGGTCGTCGTTGACCATACCATCTGCACCTGTGTATTACCCTAGTGAGGATGAGTTTAGGGATCCTTTGGAGTATATATGTAAGATCAGGGCTGAGGCTGAGCCCTACGGGATTTGTAGGATAGTTCCGCCGAGTAGCTGGAAGCCCCCATTTGCTTTGGACTTGGATTCGTTTACATTTCCGACGAAAACGCAGGCTATTCACCAGTTGCAGGTGAGGCCTGCGTCGTGTGATTCGAAGACTTTTGAGTTGGAGTACAATAGATTTTTGGAGGATCACTGTGGGAAGAGGTTGAGGGGGAAGGTGGTTTTTGAAGGGGAGGAGTTGGATTTGTGTAAGCTTTTTAATGCTGCGAAGAGGTATGGGGGGTATGATAAGGTTGTGAAGGAGAAGAAGTGGGGGGAGGTTGTGAGGTTTGTGAGGCCGGCGAGGAAGGTTTCTGAGTGTTCGAAGCATGTGTTGCATCAGTTGTATTTGGAGCATTTGGTTGAGTATGAGGAGTATTATAATAAGCTGAATAAAGAGGGGGGTAGGGGTTGTAAGAGGGGTTTGCAGGAGGAGAAGAAGGGTGAACATAATGCTGAATGTTCTAGCTCCAAAAGGAGGCGAACGAATAATAATGATAGTGAGAGGTCTAGGGTTCGAAAGGTAAAGAAGGAAGAGGAGGAACATGATCAGATATGTGAGCAATGCAGAAGTGGGTTGCATGGGGAAGTGATGCTTTTGTGTGATAGATGTGATAAGGGGTGGCACATATACTGTCTCTCCCCGCCTTTGAAACAGATCCCGTCAGGGAATTGGTACTGCCTAGATTGCTTGAATTCTGATGAAGATTGTTTTGGTTTTGTGCCCGGAAAGCGTTTATCACTGGAAGCTTTTAGGCGCGTGGCTGATCGGGCCAAGAAGAAATGGTTTGGGGCAGGACCTGCGTCTCGGGTGCAAATAGAGAAAAAGTTCTGGGAAATTGTGGAGGGTTCCATCGGTGAGGTTGAAGTTATGTATGGCAGCGACCTGGATACTTCCATTTATGGGAGCGGTTTTCCGCGTGTAAATGACCAGAGACAAGAGTCAGTTGAGGCCAAGATATGGGATGAATATTGCAGTAGCCCTTGGAATCTCAATAACTTGCCCAAGTTGAAAGGATCAGTTCTTAGAGCTGTTCATAATAACATTACTGGTGTTATGGTGCCTTGGCTCTATATTGGGATGCTGTTTTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTATTCAATGAATTATCATCACTGGGGAGAGCCCAAATGCTGGTACAGCGTCCCTGGTAGTGAAGCCAGTGCCTTTGAAAAGGTGATGCGTAACAGTCTTCCTGATCTTTTTGATGCACAACCTGATTTGCTCTTTCAGCTTGTCACTATGTTAAATCCATCTGTTTTGCAAGAGAATGGAGTTCCTGTATACAGCGTCCTACAGGAGCCTGGAAATTTTGTCATCACCTTCCCCAGGTCTTACCATGGAGGTTTTAATCTTGGTTTGAATTGTGCAGAGGCTGTCAATTTTGCCCCTGCTGACTGGTTACCTCATGGTGGGTTTGGAGCAGGGCTCTATCAGCGTTATCATAAAACTGCAGTTCTGTCTCACGAGGAGCTCGTCTGTGTGCTAGCCAAGAGTGGCTGTGACAGCAGAGTATCACCTTATTTGAAGAAAGAATTGATAAGAATATATAACAAGGAGAAAACCTGGAGAGAGCGCCTTTGGAAAAAAGGAATCACCAAATCATCTCTTATGTCTTCTCGGAAGTTCCCTGAATATGTTGGTACTGAGGAGGATCGTACGTGCATTATATGCCAGCAATATCTTTACCTCTCTGGTGTGGTTTGTCATTGTCGACCATCCACATTTGTATGTCTGGAGCACTCAGAACGTCTTTGTGAATGCAAATCCAGCAGACTCCGTCTTCACTATCGCCATACACTTGCAGAATTGCATGACTTGGTGCTTGCAATGGATAAACATTGTGGTGAGGAGACAGCACAAAGTAGAACAAAAAAAAGACAACTCCAATGCTCCAATGAACCAAGTGCTTTAACAAAAAAGGTCAAGGGTGCTCATGTTAATTTTGCTCAACTTGCGGACAAATGGCTTTTGCGCTCATCCAAGATTTTCCATAGTCGGTTTTCCAGAGAAGACTATGTTACTATATTGAAAGAAGCTGAACAATTTGTTTGGGCTGGTTCAGAGATGAATAATGTTCGGGAAATGGCAAATAATTTGAAAGAAGCTCGGAAATGGGCAGAGGGTGTGAGAAAGTCTGTATCTAAAATTGAAAGTTGGTCATCTCATCGCGACAAAGATATAGAGAAGATTCACTTGGAATATATCAATGAGTTGCTGAGTTTTGATTTGTTGCCCTGTGATGAGCCTGGGCATCTTATTTTGAAGGATTATGCTGAAAAGGCAAGGATACTGATAGAGGAAATCAATACTGCTATGTCTTCATGCCCAAAAATCCCTGAGTTGGAATTGTTGTACTACAGAGTTTGTGAGTTCCCTATTTATGTAAAAGAAAGTGAAAGGTTACAACACAAAATTTCATCTGCAAAGGTTTGGATAGAAGGCATAACAAAATGCATCTCGGAGAAACAGCCTGCTGCAATTGAGCTTGATGTTCTTTACAAGTTGAAATTGGAGATCCCAGAAATCGAAGTTCAACTCCCACAGATAGAAATGCTTTCTGATATAGTATGGAAAGCTGATTCATGTCGAGCTCAATGTGTTGAAATTTTGAAAGGTCCTATTGGCCTCAAGGATATTGAGGTGCTTCTTCTAGAATGGGATGCTTTTACTGTCAACATACCAGAGTTGAAGCTTTTGAGGCAGTACCACACTGATGCTGTTTCTTGGAATGCTCGCTTTGAAGCTGTTCTCATGAAAGTTCATGAAAGAGAGGATCAACATAATGTAGTTGATGAGTTAGAGCACATTCTAAAGGATGGGGCAACTTTGAAAATCCAAGTTGATCAGATGCCTTTGGTTGAGTTTGAGTTGAAAAAGGCTCAGTGCAGGGAAAAGGCTTTGAGGACACGAGAATCGAAATTGTCTCTGGACTTCATTCAAGAAGTGATGGTGGATGCACAAGGTTTACATATTGAGGGAGAACAAATATATGTTGATATGTCCAAAGTACTTGATGCTGCCATCCAATGGGAGGAAAGAGCAAAATATATTCTTGCACATGGAGCTCAAATTTCTGACTTTGAGGATGTTCTTAGGAGTTCGGAAAACATACATGTGACTTTACCTTCTCTCCTTGATGTCAAGGAGGCACTATCAAAGGCTATGGCCTGGTTGAGTAGATCTGAGCCGTTTCTTGTGCATTGTTCTTCGTTGGAATCTGGCTCAAGTTCTTTGCTGAAAGTTCACACCTTGAAGGAGTTGATTTCTGAGTCGAAGAATCTTAAAGTATCTATGAAAGAAATTAAGATTCTTGAGACTGTTTTGGTGAACTGTGAGGAATGGGAGCATGATGCTTGCTCTCTACTGCAAGATTCAAGATGCCTATTGGATATGACGGCCATTGGTGAGGGGATAAGTGAGGGACTTATTTCCAAGATTGAAAATGTACTTGCAAGAATAGGATCCATGGAAAACACTGGTTTATCTCTTACTTTTGACTTTGTTGAGCTTGCAAAACTCAAGGATGCTTGTTCTCTGCTGCAATGGTGTAAGAAGGCTATCTCTTTCTGCTCTGCTGCTCCTACTCTTGAGGATGTTGAGAGTTTGATGAGTGATGCGGAGACTTCATGTTGTACAAATGCCTCTGGTGCACTCTGTGACTTGTTGTTTGAAGGAGTCAAATGGCTTAGGCAAGCTACAAAGATTATTTCTGCATCTAGGAATTCTACAAGATGCAAGTTGAGTGAAGCTGAAGAAGTTCTTACTGATTCCCAGAGTATTAGTATCTCCTTCCCTCTGACAGTTACTCAAATTGAAACTGCCATTGAGAGACATAAGTCTTGGCTAGAGCAAGTGCATCAATTTTTCAGCCTAAGGCTGGGGGAACGATCTTGGTCTGTTATATTACAGCTAAAGGAGCTTGGGATTGCTGGTGCTTTCTATTGCCCAGAACTAGATTCAATATTATCTGAAGTTGAAAGGGTTCAGAAGTGGAAGCGACAATGCATGGATATTTTCAGGATTGTAGAGGAGAATTCACTGCTTGGTTCTCTAGAAAAGATACGACAGAATCTGGATAGATCTCTGCATATATATGACAAGGCACATGGTTTGAAAGAGGAAGGTTCTTATGCGTGCTGCTCCATTGGTTCTCTGGATCAGGAGTTTCTTACCTGTTCTTTGTGCAAGGAATGCTACCATTTGCGGTGCCTAGGATCTTTGACAGTAGATGGGAAACAGTCAGCATATGTGTGCCTGTGTTGCCAGTATCTAGTATCTGGAACTTTGCAAAATGGAGGAAGTCCTCGGGGATTTGGAGGAGCTCGTCCAGCACTGCAAAAGATTATTGAACTCTTATCTGATGAAGAGGATTTCTGTGTTTGTATGGAAGAAAGAAAGATGTTGAAAGAGGTTTTGAAGCAAGCTCGGGAATGCAAGAGACACTTGGAAGGAATAGTGGGTTTTGCATTAGCTTATTCGGACAAAGATCTGAGTGTCCTCTTCGAAAAACTAGCCACTGCTTTAAAGGCCATAGAGTTGGGAGGTTTTTATGACGATGAAGGTTATTGCAACCTTACATTGGCAATATCAAGATACTCGTGGAAAGTCAAAGTTCATAGATTACTCGAGGGTTCACAAAAGCCCACAATCCAACAGATTCAACAGCATTTAAAAGAGAGAGTGGCCGTGAACATTCCATCTGAAGATTACTTTAAGCAAAAACTTACGGAACTGAAGTGCAGTGGCTTGCAGTGGGCAGATAAGGCTAAGAAGGTTGCTGCTGATTCTGGGGCTCTCCCTCTGGAGAAAGTTTTTGAACTTATTTCTGAGGGTGAAAACTTACCTGTTCTTGTAGAGAAGGAACTTAAGCTATTGAAAGATCGGAGTATGCTTTATTGCATATGTAGAAAGCCTTATGATAAGAGAGCAATGATTGCATGTGACAAGTGTGATGAGTGGTATCACTTTGGTTGTGTGAAATTACGTTCCACACCGAAGGTTTACATTTGCCCTGCATGTGAGCCTCTAGCAGAGACATTGTCTTCATCAGCAGTAGTTGATAGTGAGAGATGTGCTGATGCCAAATTTGTAGAACCAAAAACACCCTCACCTAAGCACACAAAGCCCAGGATGAGCCCAAACAAAGATGAGTTTATTGCAACACAAAAGATGGTTGCAAGAACAGATGACGCCAATGTTTTTAGGTCTTCTAGTGGGATAGACCGTTTATGGTGGCGAAATCGGAAGCCTTTCAGAAGGGCGGCTAAAAAACGAGCTGAGCTTGATAGTCTCTCTCTATTTTCTCATTTGCAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1851

Amino Acids

210.96

Weight (kDa)

6.55

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 36 - 69 1.8e-16 jmjN domain
ARID PF01388 107 - 192 4.1e-10 ARID/BRIGHT DNA binding domain
PHD PF00628 254 - 298 8.3e-09 PHD-finger
JmjC PF02373 424 - 540 3.7e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 633 - 685 6.6e-14 C5HC2 zinc finger
PLU-1 PF08429 958 - 1193 4.7e-19 PLU-1-like protein
PLU-1 PF08429 1153 - 1418 9e-16 PLU-1-like protein
PHD PF00628 1712 - 1752 5.6e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 2 cut(s) 280, 1012
AccB1I GGYRCC 2 cut(s) 1264, 4443
AccB7I CCANNNNNTGG 3 cut(s) 1164, 1368, 3264
AccBSI CCGCTC 1 cut(s) 1113
AccI GTMKAC 2 cut(s) 1518, 1948
AccII CGCG 3 cut(s) 963, 1123, 2406
AciI CCGC 8 cut(s) 206, 848, 1113, 1121, 2192, 3854, 4441, 5498
AcoI YGGCCR 2 cut(s) 3633, 4920
AcsI RAATTY 8 cut(s) 898, 1543, 2649, 2841, 3306, 3958, 4270, 5321
AcuI CTGAAG 4 cut(s) 4008, 4227, 4959, 4991
AcyI GRCGYC 1 cut(s) 5427
AdeI CACNNNGTG 1 cut(s) 3893
AflII CTTAAG 1 cut(s) 5102
AflIII ACRYGT 1 cut(s) 3349
AjnI CCWGG 8 cut(s) 1089, 1381, 1537, 1562, 1799, 2482, 3404, 5363
AjuI GAANNNNNNNTTGG 4 cut(s) 900, 932, 2414, 2446
Alw21I GWGCWC 8 cut(s) 1710, 1977, 2170, 2830, 3042, 3304, 3889, 4561
Alw44I GTGCAC 1 cut(s) 3885
AlwNI CAGNNNCTG 3 cut(s) 1004, 1610, 4309
Ama87I CYCGRG 4 cut(s) 1011, 4542, 4662, 4862
ApaI GGGCCC 1 cut(s) 48
ApaLI GTGCAC 1 cut(s) 3885
ApeKI GCWGC 8 cut(s) 428, 1083, 2711, 3254, 3775, 3812, 4374, 5015
ApoI RAATTY 8 cut(s) 898, 1543, 2649, 2841, 3306, 3958, 4270, 5321
ArsI GACNNNNNNTTYG 2 cut(s) 3887, 3919
Asp700I GAANNNNTTC 5 cut(s) 1031, 2982, 3993, 4218, 5486
AspA2I CCTAGG 2 cut(s) 17, 4447
AspLEI GCGC 3 cut(s) 963, 1812, 2211
AspS9I GGNCC 5 cut(s) 44, 45, 975, 1001, 2852
AsuC2I CCSGG 1 cut(s) 930
AsuII TTCGAA 4 cut(s) 314, 527, 721, 4740
AsuNHI GCTAGC 1 cut(s) 1720
AvaI CYCGRG 4 cut(s) 1011, 4542, 4662, 4862
AvaII GGWCC 2 cut(s) 1001, 2852
AvrII CCTAGG 2 cut(s) 17, 4447
AxyI CCTNAGG 1 cut(s) 4109
BaeGI GKGCMC 3 cut(s) 48, 930, 3889
BamHI GGATCC 2 cut(s) 139, 3698
BanI GGYRCC 2 cut(s) 1264, 4443
BauI CACGAG 3 cut(s) 1700, 3144, 4834
Bbv12I GWGCWC 8 cut(s) 1710, 1977, 2170, 2830, 3042, 3304, 3889, 4561
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 8 cut(s) 415, 1095, 2698, 3241, 3762, 3799, 4361, 5002
BceAI ACGGC 3 cut(s) 3409, 3648, 4907
BciT130I CCWGG 8 cut(s) 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
BciVI GTATCC 2 cut(s) 1087, 2517
BclI TGATCA 2 cut(s) 751, 3079
BcnI CCSGG 1 cut(s) 930
BfmI CTRYAG 3 cut(s) 1529, 1686, 2593
BfoI RGCGCY 1 cut(s) 1813
BfrI CTTAAG 1 cut(s) 5102
BfuAI ACCTGC 2 cut(s) 280, 1012
BfuI GTATCC 2 cut(s) 1087, 2517
BglII AGATCT 3 cut(s) 3416, 4316, 4723
BisI GCNGC 9 cut(s) 429, 1084, 2712, 3255, 3776, 3813, 4375, 5016, 5499
BlnI CCTAGG 2 cut(s) 17, 4447
BlpI GCTNAGC 2 cut(s) 177, 5514
BlsI GCNGC 9 cut(s) 430, 1085, 2713, 3256, 3777, 3814, 4376, 5017, 5500
BmcAI AGTACT 1 cut(s) 3246
Bme1390I CCNGG 9 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
Bme18I GGWCC 2 cut(s) 1001, 2852
BmeT110I CYCGRG 4 cut(s) 1011, 4542, 4662, 4862
BmgT120I GGNCC 5 cut(s) 44, 45, 975, 1001, 2852
BmiI GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 3700, 4445
BmrFI CCNGG 9 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
BmrI ACTGGG 1 cut(s) 1360
BmtI GCTAGC 1 cut(s) 1724
BmuI ACTGGG 1 cut(s) 1360
BoxI GACNNNNGTC 3 cut(s) 1006, 1144, 4072
BplI GAGNNNNNCTC 2 cut(s) 1647, 1679
BpmI CTGGAG 3 cut(s) 1822, 1991, 5060
Bpu10I CCTNAGC 3 cut(s) 171, 3923, 5349
Bpu1102I GCTNAGC 2 cut(s) 177, 5514
Bpu14I TTCGAA 4 cut(s) 314, 527, 721, 4740
BpuEI CTTGAG 5 cut(s) 2849, 3445, 3569, 3740, 3845
BpuMI CCSGG 1 cut(s) 930
BsaAI YACGTR 1 cut(s) 1896
BsaHI GRCGYC 1 cut(s) 5427
BsaXI ACNNNNNCTCC 8 cut(s) 458, 488, 827, 857, 1500, 1530, 3351, 3381
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 8 cut(s) 283, 951, 1258, 1355, 1395, 1637, 3718, 4501
Bse21I CCTNAGG 1 cut(s) 4109
Bse3DI GCAATG 4 cut(s) 773, 2070, 3785, 5172
BseBI CCWGG 8 cut(s) 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
BseMI GCAATG 4 cut(s) 773, 2070, 3785, 5172
BseNI ACTGG 8 cut(s) 283, 951, 1258, 1355, 1395, 1637, 3718, 4501
BseRI GAGGAG 9 cut(s) 413, 590, 650, 755, 1718, 1898, 2099, 4280, 4569
BseSI GKGCMC 3 cut(s) 48, 930, 3889
BseXI GCAGC 8 cut(s) 415, 1095, 2698, 3241, 3762, 3799, 4361, 5002
BseYI CCCAGC 1 cut(s) 4114
BsgI GTGCAG 4 cut(s) 87, 1623, 3142, 4996
Bsh1236I CGCG 3 cut(s) 963, 1123, 2406
BshNI GGYRCC 2 cut(s) 1264, 4443
BsiHKAI GWGCWC 8 cut(s) 1710, 1977, 2170, 2830, 3042, 3304, 3889, 4561
BsiHKCI CYCGRG 4 cut(s) 1011, 4542, 4662, 4862
BsiSI CCGG 2 cut(s) 503, 930
BsiWI CGTACG 1 cut(s) 1892
BslFI GGGAC 2 cut(s) 1364, 3672
BsmBI CGTCTC 1 cut(s) 1014
BsmFI GGGAC 2 cut(s) 1364, 3672
BsmI GAATGC 4 cut(s) 2000, 2971, 4433, 4673
BsoBI CYCGRG 4 cut(s) 1011, 4542, 4662, 4862
Bsp119I TTCGAA 4 cut(s) 314, 527, 721, 4740
Bsp120I GGGCCC 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 3869
Bsp1720I GCTNAGC 2 cut(s) 177, 5514
Bsp19I CCATGG 2 cut(s) 1573, 3702
Bsp68I TCGCGA 1 cut(s) 2406
BspACI CCGC 8 cut(s) 206, 848, 1113, 1121, 2192, 3854, 4441, 5498
BspFNI CGCG 3 cut(s) 963, 1123, 2406
BspHI TCATGA 2 cut(s) 2988, 2998
BspLI GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 3700, 4445
BspMAI CTGCAG 1 cut(s) 1690
BspMI ACCTGC 2 cut(s) 280, 1012
BspOI GCTAGC 1 cut(s) 1724
BspT104I TTCGAA 4 cut(s) 314, 527, 721, 4740
BspT107I GGYRCC 2 cut(s) 1264, 4443
BspTI CTTAAG 1 cut(s) 5102
BsrBI CCGCTC 1 cut(s) 1113
BsrDI GCAATG 4 cut(s) 773, 2070, 3785, 5172
BsrFI RCCGGY 1 cut(s) 502
BsrGI TGTACA 1 cut(s) 3869
BsrI ACTGG 8 cut(s) 283, 951, 1258, 1355, 1395, 1637, 3718, 4501
BssAI RCCGGY 1 cut(s) 502
BssNAI GTATAC 1 cut(s) 1519
BssNI GRCGYC 1 cut(s) 5427
BssSI CACGAG 3 cut(s) 1700, 3144, 4834
BssT1I CCWWGG 6 cut(s) 17, 1187, 1268, 1573, 3702, 4447
Bst1107I GTATAC 1 cut(s) 1519
Bst2BI CACGAG 3 cut(s) 1700, 3144, 4834
Bst2UI CCWGG 8 cut(s) 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
Bst6I CTCTTC 5 cut(s) 359, 428, 732, 4596, 4742
BstACI GRCGYC 1 cut(s) 5427
BstAFI CTTAAG 1 cut(s) 5102
BstAPI GCANNNNNTGC 5 cut(s) 538, 1004, 1905, 4428, 5171
BstAUI TGTACA 1 cut(s) 3869
BstBAI YACGTR 1 cut(s) 1896
BstBI TTCGAA 4 cut(s) 314, 527, 721, 4740
BstDSI CCRYGG 2 cut(s) 1573, 3702
BstEII GGTNACC 1 cut(s) 1635
BstENI CCTNNNNNAGG 1 cut(s) 1561
BstFNI CGCG 3 cut(s) 963, 1123, 2406
BstH2I RGCGCY 1 cut(s) 1813
BstHHI GCGC 3 cut(s) 963, 1812, 2211
BstNI CCWGG 8 cut(s) 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
BstNSI RCATGY 4 cut(s) 536, 3353, 5178, 5256
BstPAI GACNNNNGTC 3 cut(s) 1006, 1144, 4072
BstPI GGTNACC 1 cut(s) 1635
BstSCI CCNGG 9 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2482, 3404, 5363
BstSFI CTRYAG 3 cut(s) 1529, 1686, 2593
BstSLI GKGCMC 3 cut(s) 48, 930, 3889
BstUI CGCG 3 cut(s) 963, 1123, 2406
BstV1I GCAGC 8 cut(s) 415, 1095, 2698, 3241, 3762, 3799, 4361, 5002
BstX2I RGATCY 8 cut(s) 139, 861, 2751, 3416, 3698, 4316, 4451, 4723
BstXI CCANNNNNNTGG 1 cut(s) 4774
BstYI RGATCY 8 cut(s) 139, 861, 2751, 3416, 3698, 4316, 4451, 4723
BstZ17I GTATAC 1 cut(s) 1519
Bsu36I CCTNAGG 1 cut(s) 4109
BsuI GTATCC 2 cut(s) 1087, 2517
BtgI CCRYGG 2 cut(s) 1573, 3702
BtgZI GCGATG 1 cut(s) 2387
BtsI GCAGTG 5 cut(s) 4274, 4568, 4752, 4984, 4994
BtuMI TCGCGA 1 cut(s) 2406
BveI ACCTGC 2 cut(s) 280, 1012
CaiI CAGNNNCTG 3 cut(s) 1004, 1610, 4309
CciI TCATGA 2 cut(s) 2988, 2998
CfoI GCGC 3 cut(s) 963, 1812, 2211
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 5 cut(s) 44, 45, 975, 1001, 2852
CseI GACGC 5 cut(s) 291, 996, 1366, 1513, 5435
DraI TTTAAA 2 cut(s) 4761, 4908
DraIII CACNNNGTG 1 cut(s) 3893
EaeI YGGCCR 2 cut(s) 3633, 4920
Eam1104I CTCTTC 5 cut(s) 359, 428, 732, 4596, 4742
EarI CTCTTC 5 cut(s) 359, 428, 732, 4596, 4742
EciI GGCGGA 1 cut(s) 195
Ecl136II GAGCTC 4 cut(s) 1708, 2828, 3302, 4559
Eco130I CCWWGG 6 cut(s) 17, 1187, 1268, 1573, 3702, 4447
Eco147I AGGCCT 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 1001, 2852
Eco53kI GAGCTC 4 cut(s) 1708, 2828, 3302, 4559
Eco57I CTGAAG 4 cut(s) 4008, 4227, 4959, 4991
Eco81I CCTNAGG 1 cut(s) 4109
Eco88I CYCGRG 4 cut(s) 1011, 4542, 4662, 4862
Eco91I GGTNACC 1 cut(s) 1635
EcoICRI GAGCTC 4 cut(s) 1708, 2828, 3302, 4559
EcoNI CCTNNNNNAGG 1 cut(s) 1561
EcoO109I RGGNCCY 3 cut(s) 44, 1001, 2852
EcoO65I GGTNACC 1 cut(s) 1635
EcoRI GAATTC 3 cut(s) 898, 3958, 4270
EcoRII CCWGG 8 cut(s) 1089, 1381, 1537, 1562, 1799, 2482, 3404, 5363
EcoT14I CCWWGG 6 cut(s) 17, 1187, 1268, 1573, 3702, 4447
EcoT22I ATGCAT 2 cut(s) 2693, 4244
ErhI CCWWGG 6 cut(s) 17, 1187, 1268, 1573, 3702, 4447
Esp3I CGTCTC 1 cut(s) 1014
FalI AAGNNNNNCTT 8 cut(s) 783, 815, 2414, 2446, 3981, 4013, 4664, 4696
FaqI GGGAC 2 cut(s) 1364, 3672
FauI CCCGC 1 cut(s) 855
FauNDI CATATG 2 cut(s) 4483, 5138
FbaI TGATCA 2 cut(s) 751, 3079
FblI GTMKAC 2 cut(s) 1518, 1948
Fnu4HI GCNGC 9 cut(s) 429, 1084, 2712, 3255, 3776, 3813, 4375, 5016, 5499
Fsp4HI GCNGC 9 cut(s) 429, 1084, 2712, 3255, 3776, 3813, 4375, 5016, 5499
GlaI GCGC 3 cut(s) 962, 1811, 2210
GluI GCNGC 9 cut(s) 429, 1084, 2712, 3255, 3776, 3813, 4375, 5016, 5499
GsaI CCCAGC 1 cut(s) 4118
GsuI CTGGAG 3 cut(s) 1822, 1991, 5060
HaeII RGCGCY 1 cut(s) 1813
HapII CCGG 2 cut(s) 503, 930
HgaI GACGC 5 cut(s) 291, 996, 1366, 1513, 5435
HhaI GCGC 3 cut(s) 963, 1812, 2211
Hin1I GRCGYC 1 cut(s) 5427
Hin6I GCGC 3 cut(s) 961, 1810, 2209
HinP1I GCGC 3 cut(s) 961, 1810, 2209
HincII GTYRAC 3 cut(s) 90, 1949, 2911
HindII GTYRAC 3 cut(s) 90, 1949, 2911
HindIII AAGCTT 3 cut(s) 416, 951, 2927
HpaII CCGG 2 cut(s) 503, 930
Hpy99I CGWCG 3 cut(s) 88, 265, 307
HpyCH4IV ACGT 3 cut(s) 1895, 1984, 5221
HpySE526I ACGT 3 cut(s) 1895, 1984, 5221
Hsp92I GRCGYC 1 cut(s) 5427
HspAI GCGC 3 cut(s) 961, 1810, 2209
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Ksp22I TGATCA 2 cut(s) 751, 3079
Lsp1109I GCAGC 8 cut(s) 415, 1095, 2698, 3241, 3762, 3799, 4361, 5002
MaeII ACGT 3 cut(s) 1895, 1984, 5221
MbiI CCGCTC 1 cut(s) 1113
MfeI CAATTG 1 cut(s) 2715
MflI RGATCY 8 cut(s) 139, 861, 2751, 3416, 3698, 4316, 4451, 4723
MlyI GAGTC 4 cut(s) 1154, 2004, 3515, 3921
MmeI TCCRAC 6 cut(s) 284, 309, 384, 2559, 3429, 4912
Mph1103I ATGCAT 2 cut(s) 2693, 4244
MroNI GCCGGC 1 cut(s) 502
MroXI GAANNNNTTC 5 cut(s) 1031, 2982, 3993, 4218, 5486
MslI CAYNNNNRTG 6 cut(s) 1963, 2079, 2949, 3350, 4329, 4486
MspCI CTTAAG 1 cut(s) 5102
MspI CCGG 2 cut(s) 503, 930
MspR9I CCNGG 9 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
MunI CAATTG 1 cut(s) 2715
Mva1269I GAATGC 4 cut(s) 2000, 2971, 4433, 4673
MvaI CCWGG 8 cut(s) 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
MvnI CGCG 3 cut(s) 963, 1123, 2406
NaeI GCCGGC 1 cut(s) 504
NciI CCSGG 1 cut(s) 930
NcoI CCATGG 2 cut(s) 1573, 3702
NdeI CATATG 2 cut(s) 4483, 5138
NgoMIV GCCGGC 1 cut(s) 502
NheI GCTAGC 1 cut(s) 1720
NlaIV GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 3700, 4445
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 5 cut(s) 1471, 1736, 3352, 3893, 5177
NruI TCGCGA 1 cut(s) 2406
NsiI ATGCAT 2 cut(s) 2693, 4244
NspI RCATGY 4 cut(s) 536, 3353, 5178, 5256
NspV TTCGAA 4 cut(s) 314, 527, 721, 4740
PaeR7I CTCGAG 1 cut(s) 4862
PagI TCATGA 2 cut(s) 2988, 2998
PaqCI CACCTGC 1 cut(s) 280
PceI AGGCCT 1 cut(s) 298
PciI ACATGT 1 cut(s) 3349
PcsI WCGNNNNNNNCGW 4 cut(s) 71, 80, 311, 1707
PctI GAATGC 4 cut(s) 2000, 2971, 4433, 4673
PdiI GCCGGC 1 cut(s) 504
PdmI GAANNNNTTC 5 cut(s) 1031, 2982, 3993, 4218, 5486
Pfl23II CGTACG 1 cut(s) 1892
PflFI GACNNNGTC 2 cut(s) 2013, 5275
PflMI CCANNNNNTGG 3 cut(s) 1164, 1368, 3264
PkrI GCNGC 9 cut(s) 430, 1085, 2713, 3256, 3777, 3814, 4376, 5017, 5500
PleI GAGTC 4 cut(s) 1153, 2004, 3514, 3920
PpsI GAGTC 4 cut(s) 1153, 2004, 3514, 3920
Ppu21I YACGTR 1 cut(s) 1896
PpuMI RGGWCCY 2 cut(s) 1001, 2852
PscI ACATGT 1 cut(s) 3349
PshAI GACNNNNGTC 3 cut(s) 1006, 1144, 4072
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 4 cut(s) 1710, 2830, 3304, 4561
Psp5II RGGWCCY 2 cut(s) 1001, 2852
Psp6I CCWGG 8 cut(s) 1089, 1381, 1537, 1562, 1799, 2482, 3404, 5363
PspEI GGTNACC 1 cut(s) 1635
PspFI CCCAGC 1 cut(s) 4114
PspGI CCWGG 8 cut(s) 1089, 1381, 1537, 1562, 1799, 2482, 3404, 5363
PspLI CGTACG 1 cut(s) 1892
PspN4I GGNNCC 8 cut(s) 45, 46, 141, 1054, 1266, 1536, 3700, 4445
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 5 cut(s) 44, 45, 975, 1001, 2852
PspPPI RGGWCCY 2 cut(s) 1001, 2852
PspXI VCTCGAGB 1 cut(s) 4862
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 3 cut(s) 1004, 1610, 4309
PsuI RGATCY 8 cut(s) 139, 861, 2751, 3416, 3698, 4316, 4451, 4723
PsyI GACNNNGTC 2 cut(s) 2013, 5275
RruI TCGCGA 1 cut(s) 2406
RseI CAYNNNNRTG 6 cut(s) 1963, 2079, 2949, 3350, 4329, 4486
SacI GAGCTC 4 cut(s) 1710, 2830, 3304, 4561
SalI GTCGAC 1 cut(s) 1947
SatI GCNGC 9 cut(s) 429, 1084, 2712, 3255, 3776, 3813, 4375, 5016, 5499
Sau96I GGNCC 5 cut(s) 44, 45, 975, 1001, 2852
ScaI AGTACT 1 cut(s) 3246
SchI GAGTC 4 cut(s) 1154, 2004, 3515, 3921
ScrFI CCNGG 9 cut(s) 930, 1091, 1383, 1539, 1564, 1801, 2484, 3406, 5365
SfcI CTRYAG 3 cut(s) 1529, 1686, 2593
Sfr274I CTCGAG 1 cut(s) 4862
SfuI TTCGAA 4 cut(s) 314, 527, 721, 4740
SinI GGWCC 2 cut(s) 1001, 2852
SlaI CTCGAG 1 cut(s) 4862
SmiMI CAYNNNNRTG 6 cut(s) 1963, 2079, 2949, 3350, 4329, 4486
SmlI CTYRAG 7 cut(s) 2864, 3460, 3548, 3755, 3824, 4862, 5102
SmoI CTYRAG 7 cut(s) 2864, 3460, 3548, 3755, 3824, 4862, 5102
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 8 cut(s) 206, 848, 1113, 1121, 2192, 3854, 4441, 5498
SspI AATATT 2 cut(s) 1175, 4200
SstI GAGCTC 4 cut(s) 1710, 2830, 3304, 4561
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 9 cut(s) 928, 1089, 1381, 1537, 1562, 1799, 2482, 3404, 5363
StyI CCWWGG 6 cut(s) 17, 1187, 1268, 1573, 3702, 4447
TaiI ACGT 3 cut(s) 1898, 1987, 5224
TatI WGTACW 5 cut(s) 333, 2589, 3244, 3682, 3869
TauI GCSGC 1 cut(s) 5501
TseFI GTSAC 5 cut(s) 1471, 1736, 3352, 3893, 5177
TseI GCWGC 8 cut(s) 428, 1083, 2711, 3254, 3775, 3812, 4374, 5015
Tsp45I GTSAC 5 cut(s) 1471, 1736, 3352, 3893, 5177
TspGWI ACGGA 2 cut(s) 2003, 4979
Tth111I GACNNNGTC 2 cut(s) 2013, 5275
Van91I CCANNNNNTGG 3 cut(s) 1164, 1368, 3264
Vha464I CTTAAG 1 cut(s) 5102
VneI GTGCAC 1 cut(s) 3885
VpaK11BI GGWCC 2 cut(s) 1001, 2852
XagI CCTNNNNNAGG 1 cut(s) 1561
XapI RAATTY 8 cut(s) 898, 1543, 2649, 2841, 3306, 3958, 4270, 5321
XbaI TCTAGA 2 cut(s) 2887, 4288
XceI RCATGY 4 cut(s) 536, 3353, 5178, 5256
XcmI CCANNNNNNNNNTGG 1 cut(s) 4387
XhoI CTCGAG 1 cut(s) 4862
XmaJI CCTAGG 2 cut(s) 17, 4447
XmiI GTMKAC 2 cut(s) 1518, 1948
XmnI GAANNNNTTC 5 cut(s) 1031, 2982, 3993, 4218, 5486
ZrmI AGTACT 1 cut(s) 3246
Zsp2I ATGCAT 2 cut(s) 2693, 4244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.