Rroxscaffold_3G00258150

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004720
Physical Location & Seq
Reverse (-)
52462567 .. 52477343
14777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258150.1

Sequence Viewer

Length: 5508 bp
ATGGGTAAGGGAAGACCTAGGGCTGTGGAGAAGGGGGTGGTGGGGCCCAATTTGAGTGCTTCATCGTCTTCTTCGTCTGGGTCGTCGTTGACCATACCATCTGCACCTGTGTATTACCCTAGTGAGGATGAGTTTAGGGATCCTTTGGAGTATATATGTAAGATCAGGGCTGAGGCTGAGCCCTATGGGATTTGTAGGATAGTTCCGCCGAGTAGCTGGAAGCCCCCATTTGCTTTGGACTTGGATTCGTTTACATTTCCGACGAAGACGCAGGCTATTCACCAGTTGCAGGTGAGGCCTGCGTCGTGTGATTCGAAGACTTTTGAGTTGGAGTACAATAGGTTTTTGGAGGATCATTGTGGGAAGAGGTTGAGGAGGAAGGTGGTTTTTGAAGGGGAGGAGCTGGATTTGTGTAAGCTGTTTAATGCGGCGAAGAGGTATGGGGGGTATGATAAGGTTGTGAAGGAGAAGAAGTGGGGGGAGGTTGTGAGGTTTGTGAGGCCTGCGAGGAAGGTTTCTGAGTGTTCGAAGCATGTGTTGCATCAGTTGTATTTGGAGCATTTGTTTGAGTATGAGGAGTATTATAATAAGCTGAATAAAGAGGGGGTAGGGCTTGTAAGAGGGGTTTGCAGGAGGAGAAGAAGGGTGAACATAATGCTGAATGTAAAGAAGGAAGAGGAGGAACATGATCAGATATGTGAGCAATGCAGAAGTGGGTTGCATGGGGAAGTGATGCTTTTGTGTGATAGGTGTGATAAGGGGTGGCACATATACTGTCTCTCCCCGCCTGTGAAACAGATCCCGTCAGGGAATTGGTACTGCCTAGATTGCTTGAATTCCGATGAAGATTGTTTTGGTTTTGTGCCCGGAAAGCGTTTATCACTGGAAGCTTTTAGGCGCGTGGCTGATCGGGCCAAGAAGAAATGGTTTGGGCCAGGACCTGCGTCTCGGGTGCAAATAGAGAAAAAGTTCTGGGAAATTGTGGAGGGTTCCATCGGTGAGGTTGAAGTTATGTATGGCAGCGACCTGGATACTTCCATTTATGGGAGCGGTTTTCCGCGTGTAAATGACCAGAGACAAGAGTCAGTTGAGGCCAAGATATGGGATGAATATTGCAGTAGCCCATGGAATCTCAATAACTTGCCCAAGTTGAAAGGATCAGTTCTTAGAGCTGTTCAGAATAACATTACTGGTGTTATGGTGCCTTGGCTCTATATTGGGATGCTGTTTTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTATTCAATGAATTATCATCACTGGGGAGAGCCCAAATGCTGGTACAGTGTCCCTGGTAGTGAAGCCAGTGCCTTTGAAAAGGTGATGCGTAACAGTCTTCCTGATCTTTTTGATGCACAACCTGATTTGCTCTTTCAGCTTGTCACTATGTTAAATCCATCTGTTTTGCAAGAGAATGGAGTTCCTGTATACAGCGTCCTACAGGAGCCTGGAAATTTTGTCATCACCTTCCCCAGGTCTTACCATGGAGGTTTTAATCTTGGTTTGAATTGTGCAGAGGCTGTCAATTTTGCCCCTGCTGACTGGTTACCTCATGGTGGGTTTGGAGCAGGGCTCTATCAGCTTTATCATAAAACTGCAGTTCTGTCTCACGAGGAGCTTGTTTGTGTGCTAGCCAAGAGTGACTGTGACAGCAGAGTATCACCTTATTTGAAGAAAGAATTGATAAGAATATATATCAAGGAGAAAACCTGGAGAGAGCGCCTTTGGAAAAAAGGAATCGCCAAATCATCTCTTATGCCTTCTCGGAAGTTCCCTGAATATGTTGGTACTGAGGAGGATCCTACATGCATTATATGCCAGCAATATCTTTACCTCTCCGGTGTGGTTTGTCGTTGTCGACCATCCACATTTGTATGTCTGGAGCACTCGGAACGTCTTTGTGAATGCAAATCCAGCAGACTCCGTCTTCATTATCGCCATACACTTGCAGAATTGCACGACTTGGTGCTTGCAATGGATAAACATTGTGGTGAGGAGACAACACAAAGTAGAACAAAAAAAAGACAACTCCAATGCTCCAATGAACCGACTGCATTAACAAAGAAGGTCAAGGGTGGTCATGTTAATTTTGCTCAACTTGCGGGCAAATGGCTTTTGCACTCATCCAAGATTTTCCATAGTCGGTTTTCCAGAGAAGACTATGTTACTCTATTGAAAGAAGCTGAACAATTTGTTTGGGCTGGTTCAGAGATGAATAAAGTTCGGGAAATGGCAAATAATTTGAAAGAAGCTCGGAAATGGGCAGAGGGTGTGAGAAAGTCTGTATCTAAAATTGAAAGTTGGTCATCTCATCGCGACAAAGATATAGAGAAGATTCACTTGGAATATATCAATGAGTTGCTGAGTTTTGATTTGTTGCCCTGTGATGAGCCTGAGCATCTTATTTTGAAGGATTATGCTGAAAAGGCGAGGATACTGATAGAGGAAATCAATATTGCTATGTCTTCATGCCTAAAAATCCCTGAGTTGGAATTGTTGTACAACAGAGTTTGTGAGTTCCCTATTTATGTAAAAGAAAGTGAAAGGTTACAACAGAAAATTTCATCTGCAAAGGTTTGGATAGAAGGCATAACAAAATGCATCTCGGAGAAACAGCCTGCTGCAGTTGAGCTTGATGTTCTTTACAAGTTGAAATTGGAGATCCCAGAAGTCGAAGTTCAACTCCCACAGATAGAAATGCTTTCTGATATAGTAGGGAAAGCTGAATCATGTCGAGCTCAATGTGTTGAAATTTTGAAAGGTCCTATTAGCCTCAAGGATATTGAGGTGCTTCTTCTAGAATGGGATGCTTTTACTGTCAACATACCAGAGTTGAAGCTTTTGAGGCAGTACCACACTGATGCTGTTTCTTGGAATGCTCGCTTTGAAGCTGTTCTTATGAAAGTTCATGAAAGAGAGGATCAACATAATGTAGTTGATGAGTTAGAGCACATTCTAAAGGATGGGGCAACTTTGAAAATTGAAGTTGATCAGATGCCTTTGGTTGAGTTTGAGTTGAAAAAGGCTCGGTGCAGGGAAAAGGCTTTGAGGACACGAGAATCTAAATTGTCTCTGGACTTCATTCAAGAAGTGATGGTGGATGCACAAGGTTTACATATTGAGGGAGAACAAATATATGTTGATATGTCCAAAGTACTTGATGCTGCCATCCAATGGGAGGAAAGAGCAAAATATATTCTTGCACATGGAGCTCAAATTTCTGACTTTGAGGATGTTCTTAGGAGTTCGGAAAACATACATGTGACTTTACCTTCTCTCCTTGATGTCAAGGAGGCACTATCAAAGGCTATGGCCTGGTTGAGTAGATCTGAGCCGTTTCTTGTGCATTGTTCTTCATTGGAATCTGGCTCAAGTTCTTTGCTGAAAGTTGACACCTTGAAGGAGTTGATTTCTGAGTCGAAGAATCTTAAAGTATCTATGAACGAAATTAAGATTCTTGAGACTGTTTTGATGAACTGTGAGGAATGGGAGCATGATGCTTGCTCTCTACTGCAAGATTCAAGATGCCTATTGGATATGACGACCATTGGTGAGGGGATAAGTGAGGAACTTATTTCCAAGATTGAAAATGTACTTGCAAGAATAGGATCCATGGAAAACACTGGTTTATCTCTTGCTTTTGACTTTGTTGAGCTTGTGAAACTCAAGGATGCTTGTTCTCTGCTGCAATGGTGTAAGAAGGCTATCTCTTTCTGCTCTGCTGCTCCTACTCTCGAGGATGTTGAGAGTTTGATGAGTAATGCGGAGACTTCATGTTGTACAAATGCCTCTGGTGCACTCTGTGACTTGTTGTTTGAAGGAGTCAAATGGCTTAGGCAAGCTACAAAGATTATTTCTGCATCTAGGAATTCTACAAGATGCAAGTTGAGTGAAGCTGAAGAAGTTCTTACTGATTCCCAGAGTATTAGTATCTCCTTCCCTCTGACAGTTACTCAAATTGAAACTGCCATTGAGAAACATAATGATCCGTCACACCTTTTTAATCCTCTTGATCAATATGACCCTTGGATCTTAGTATTTGGGGTCTTGGCTAGAGCAAGTGCATCAATTTTTCAGCCTAAGGCTGGGGGAGCGATCTTGGAGCTTGGGATTACTGGTGCTTTCTATTGCCCAGAACTAGATTCAATATTATCTGAAGTTGAAAGGGTTCAGAAGTGGAAGCGACAATGCATGGATATTTTCAGGATTGTAGAGGAGAATTCACTGCTTGGTTCTCTAGAAAAGATACAACAGAATCTGGATAGATCTATGCATATATATGACAAGGCACATGGTTTGAAAGAGGAAGGTTCTTATGCGTGCTGCTCCATTGGTTCTCTGGATCAGGAGTTTCTTACCTGTTCTTTGTGCAAGGAATGCTACCATTTGCGGTGCTTAGGATCTTTGACAGTAGATGGGAAACAGTCGGCATATGTGTGCCTGTGTTGCCAGTATCTAGTGTCTGGAACTTTGCAAAATGGAGGAAGTCCTCGGGGATTTGGAGGAGTTCGTCCAGCACTGCAAAAGATTATTGAACTCTTATCGGATGAAGAGGATTTCTGTGTTTGTATGGAAGAAAGAGAGATGTTGAAAGAGGTTTTGAAGCAAGCTCGGGTATGCAAGAGACACTTGGAAGGAATAGTGGATTTTGCATTAGCTTATTCGGACAAAGATCTGAGTGTCCTCTTCGAAAAACTTGCCACTGCTTTAAAGGCCATAGAGTTGGGAGGTTTTTATGACGATGATGGTTATTGCAACCTTACATTGGCAATATCAAGATACTCGTGGAAAGTCAAAGTTCATAGATTATTCGAGGGTTCACAAAAGCCCACAATCCAACAGATTCAACAGCATTTAAAAGAGAGAGTGGCGGTGAACATTCCACCTGAAGATTACTTTAAGCAAAAACTTACGGAACTGAAGTGCAGTGGCTTGCAGTGGGCAGATAAGGCTAAGAAGGTTGCTGCTGATTCTGGGGCTCTCCCTCTGGAGAAAGTTTTTGAACTTATTTCTGAGGGTGAAAACTTACCTGTTCTTGTAGAGAAGGAACTTAAGCTATTGAAAGATCGGAGTATGCTTTATTGCATATGTAGAAAGCCTTATGATAAGAGAGCAATGATTGCATGTGACAAGTGTGATGAGTGGTATCACTTTGGTTGTGTGAAATTACGTTCCACACCGAAGGTTTACATTTGCCCTGCATGTGAGCCTCTAGCAGAAACATTGTCTTCATCAGCAGTAGTTGATAGTGAGAGATGTGCTGATGCCAAATTTGTAGAACCAAAAACACCCTCTCCTAAGCACACAAAGCCCAGGATGAGCCCAAACAAATACGAGTTTATTGCAACACAAAAGATGGTTGCAAGAACAAATGACGCCAATGTTTTTAGGTCTTCTAGTGGGATAGACCGTTTATGGTGGCGAAATCGGAAGCCTTTCAGAAGGGCGGCTAAAAACGAGCCGAGCTTGATAGGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1835

Amino Acids

208.79

Weight (kDa)

6.19

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 36 - 69 1.8e-16 jmjN domain
ARID PF01388 107 - 192 1.5e-10 ARID/BRIGHT DNA binding domain
PHD PF00628 233 - 277 1.7e-08 PHD-finger
JmjC PF02373 403 - 519 3.6e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 612 - 664 7.3e-14 C5HC2 zinc finger
PLU-1 PF08429 728 - 1018 2.1e-15 PLU-1-like protein
PLU-1 PF08429 933 - 1172 4.9e-21 PLU-1-like protein
PLU-1 PF08429 1132 - 1410 1.3e-11 PLU-1-like protein
PHD PF00628 1704 - 1744 5.6e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 2 cut(s) 280, 949
AccB1I GGYRCC 1 cut(s) 1201
AccB7I CCANNNNNTGG 3 cut(s) 1101, 1305, 3201
AccBSI CCGCTC 1 cut(s) 1050
AccI GTMKAC 2 cut(s) 1455, 1885
AccII CGCG 3 cut(s) 900, 1060, 2343
AcsI RAATTY 8 cut(s) 835, 1480, 2586, 2778, 3243, 3895, 4246, 5297
AcuI CTGAAG 4 cut(s) 3945, 4203, 4935, 4967
AcyI GRCGYC 1 cut(s) 5403
AdeI CACNNNGTG 1 cut(s) 3830
AfaI GTAC 9 cut(s) 335, 818, 1310, 1816, 2528, 2879, 3183, 3621, 3808
AflII CTTAAG 1 cut(s) 5078
AflIII ACRYGT 1 cut(s) 3286
AjnI CCWGG 8 cut(s) 934, 1026, 1318, 1474, 1499, 1736, 3341, 5339
AjuI GAANNNNNNNTTGG 4 cut(s) 837, 869, 2351, 2383
Alw21I GWGCWC 5 cut(s) 1914, 2767, 2979, 3241, 3826
Alw26I GTCTC 9 cut(s) 782, 951, 1069, 1638, 2018, 3102, 3482, 3788, 4645
Alw44I GTGCAC 1 cut(s) 3822
AlwNI CAGNNNCTG 3 cut(s) 941, 1547, 4285
Ama87I CYCGRG 4 cut(s) 948, 3761, 4518, 4638
AoxI GGCC 8 cut(s) 44, 296, 500, 912, 932, 1092, 3339, 4740
ApaI GGGCCC 1 cut(s) 48
ApaLI GTGCAC 1 cut(s) 3822
ApeKI GCWGC 7 cut(s) 1020, 2648, 3191, 3712, 3749, 4350, 4991
ApoI RAATTY 8 cut(s) 835, 1480, 2586, 2778, 3243, 3895, 4246, 5297
ArsI GACNNNNNNTTYG 2 cut(s) 3824, 3856
Asp700I GAANNNNTTC 5 cut(s) 968, 2919, 3930, 4194, 5462
AspA2I CCTAGG 1 cut(s) 17
AspLEI GCGC 2 cut(s) 900, 1749
AspS9I GGNCC 6 cut(s) 44, 45, 912, 932, 938, 2789
AsuC2I CCSGG 1 cut(s) 867
AsuII TTCGAA 3 cut(s) 314, 527, 4716
AsuNHI GCTAGC 1 cut(s) 1657
AvaI CYCGRG 4 cut(s) 948, 3761, 4518, 4638
AvaII GGWCC 2 cut(s) 938, 2789
AvrII CCTAGG 1 cut(s) 17
AxyI CCTNAGG 1 cut(s) 4107
BaeGI GKGCMC 3 cut(s) 48, 867, 3826
BamHI GGATCC 3 cut(s) 139, 1825, 3635
BanI GGYRCC 1 cut(s) 1201
BanII GRGCYC 8 cut(s) 48, 183, 1299, 1602, 2767, 3241, 5008, 5351
BauI CACGAG 3 cut(s) 1637, 3081, 4810
Bbv12I GWGCWC 5 cut(s) 1914, 2767, 2979, 3241, 3826
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 7 cut(s) 1032, 2635, 3178, 3699, 3736, 4337, 4978
BceAI ACGGC 1 cut(s) 3346
BcgI CGANNNNNNTGC 4 cut(s) 4706, 4740, 5351, 5385
BciT130I CCWGG 8 cut(s) 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
BciVI GTATCC 2 cut(s) 1024, 2454
BclI TGATCA 3 cut(s) 688, 3016, 4039
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 9 cut(s) 782, 951, 1069, 1638, 2018, 3102, 3482, 3788, 4645
BfmI CTRYAG 3 cut(s) 1466, 1623, 2649
BfoI RGCGCY 1 cut(s) 1750
BfrI CTTAAG 1 cut(s) 5078
BfuAI ACCTGC 2 cut(s) 280, 949
BfuI GTATCC 2 cut(s) 1024, 2454
BglII AGATCT 3 cut(s) 3353, 4292, 4699
BisI GCNGC 9 cut(s) 429, 1021, 2649, 3192, 3713, 3750, 4351, 4992, 5475
BlnI CCTAGG 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 9 cut(s) 430, 1022, 2650, 3193, 3714, 3751, 4352, 4993, 5476
BmcAI AGTACT 1 cut(s) 3183
Bme1390I CCNGG 9 cut(s) 867, 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
Bme18I GGWCC 2 cut(s) 938, 2789
BmeT110I CYCGRG 4 cut(s) 948, 3761, 4518, 4638
BmgT120I GGNCC 6 cut(s) 44, 45, 912, 932, 938, 2789
BmiI GGNNCC 8 cut(s) 45, 46, 141, 991, 1203, 1473, 1827, 3637
BmrFI CCNGG 9 cut(s) 867, 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
BmrI ACTGGG 1 cut(s) 1297
BmtI GCTAGC 1 cut(s) 1661
BmuI ACTGGG 1 cut(s) 1297
BoxI GACNNNNGTC 2 cut(s) 943, 1081
BplI GAGNNNNNCTC 2 cut(s) 1584, 1616
BpmI CTGGAG 3 cut(s) 1759, 1928, 5036
Bpu10I CCTNAGC 5 cut(s) 171, 2421, 3860, 4423, 5325
Bpu1102I GCTNAGC 1 cut(s) 177
Bpu14I TTCGAA 3 cut(s) 314, 527, 4716
BpuEI CTTGAG 4 cut(s) 2786, 3382, 3506, 3677
BpuMI CCSGG 1 cut(s) 867
BsaHI GRCGYC 1 cut(s) 5403
BsaWI WCCGGW 1 cut(s) 1865
Bse1I ACTGG 9 cut(s) 283, 888, 1195, 1292, 1332, 1574, 3655, 4147, 4477
Bse21I CCTNAGG 1 cut(s) 4107
Bse3DI GCAATG 4 cut(s) 710, 2007, 3722, 5148
BseBI CCWGG 8 cut(s) 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
BseMI GCAATG 4 cut(s) 710, 2007, 3722, 5148
BseNI ACTGG 9 cut(s) 283, 888, 1195, 1292, 1332, 1574, 3655, 4147, 4477
BseSI GKGCMC 3 cut(s) 48, 867, 3826
BseXI GCAGC 7 cut(s) 1032, 2635, 3178, 3699, 3736, 4337, 4978
BseYI CCCAGC 1 cut(s) 4112
BsgI GTGCAG 4 cut(s) 87, 1560, 3079, 4972
Bsh1236I CGCG 3 cut(s) 900, 1060, 2343
BshFI GGCC 8 cut(s) 46, 298, 502, 914, 934, 1094, 3341, 4742
BshNI GGYRCC 1 cut(s) 1201
BsiHKAI GWGCWC 5 cut(s) 1914, 2767, 2979, 3241, 3826
BsiHKCI CYCGRG 4 cut(s) 948, 3761, 4518, 4638
BsiSI CCGG 2 cut(s) 867, 1866
BslFI GGGAC 1 cut(s) 1301
BsmAI GTCTC 9 cut(s) 782, 951, 1069, 1638, 2018, 3102, 3482, 3788, 4645
BsmBI CGTCTC 1 cut(s) 951
BsmFI GGGAC 1 cut(s) 1301
BsmI GAATGC 3 cut(s) 1937, 2908, 4409
BsnI GGCC 8 cut(s) 46, 298, 502, 914, 934, 1094, 3341, 4742
BsoBI CYCGRG 4 cut(s) 948, 3761, 4518, 4638
Bsp119I TTCGAA 3 cut(s) 314, 527, 4716
Bsp120I GGGCCC 1 cut(s) 44
Bsp1407I TGTACA 2 cut(s) 2526, 3806
Bsp1720I GCTNAGC 1 cut(s) 177
Bsp19I CCATGG 3 cut(s) 1124, 1510, 3639
Bsp68I TCGCGA 1 cut(s) 2343
BspANI GGCC 8 cut(s) 46, 298, 502, 914, 934, 1094, 3341, 4742
BspFNI CGCG 3 cut(s) 900, 1060, 2343
BspHI TCATGA 1 cut(s) 2935
BspLI GGNNCC 8 cut(s) 45, 46, 141, 991, 1203, 1473, 1827, 3637
BspMAI CTGCAG 2 cut(s) 1627, 2653
BspMI ACCTGC 2 cut(s) 280, 949
BspOI GCTAGC 1 cut(s) 1661
BspT104I TTCGAA 3 cut(s) 314, 527, 4716
BspT107I GGYRCC 1 cut(s) 1201
BspTI CTTAAG 1 cut(s) 5078
BsrBI CCGCTC 1 cut(s) 1050
BsrDI GCAATG 4 cut(s) 710, 2007, 3722, 5148
BsrGI TGTACA 2 cut(s) 2526, 3806
BsrI ACTGG 9 cut(s) 283, 888, 1195, 1292, 1332, 1574, 3655, 4147, 4477
BssNAI GTATAC 1 cut(s) 1456
BssNI GRCGYC 1 cut(s) 5403
BssSI CACGAG 3 cut(s) 1637, 3081, 4810
BssT1I CCWWGG 6 cut(s) 17, 1124, 1205, 1510, 3639, 4052
Bst1107I GTATAC 1 cut(s) 1456
Bst2BI CACGAG 3 cut(s) 1637, 3081, 4810
Bst2UI CCWGG 8 cut(s) 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
Bst6I CTCTTC 5 cut(s) 359, 428, 669, 4572, 4718
BstACI GRCGYC 1 cut(s) 5403
BstAFI CTTAAG 1 cut(s) 5078
BstAPI GCANNNNNTGC 4 cut(s) 538, 1842, 4404, 5147
BstAUI TGTACA 2 cut(s) 2526, 3806
BstBI TTCGAA 3 cut(s) 314, 527, 4716
BstDSI CCRYGG 3 cut(s) 1124, 1510, 3639
BstEII GGTNACC 1 cut(s) 1572
BstENI CCTNNNNNAGG 1 cut(s) 1498
BstFNI CGCG 3 cut(s) 900, 1060, 2343
BstH2I RGCGCY 1 cut(s) 1750
BstHHI GCGC 2 cut(s) 900, 1749
BstMAI GTCTC 9 cut(s) 782, 951, 1069, 1638, 2018, 3102, 3482, 3788, 4645
BstNI CCWGG 8 cut(s) 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
BstNSI RCATGY 5 cut(s) 536, 1836, 3290, 5154, 5232
BstPAI GACNNNNGTC 2 cut(s) 943, 1081
BstPI GGTNACC 1 cut(s) 1572
BstSCI CCNGG 9 cut(s) 865, 934, 1026, 1318, 1474, 1499, 1736, 3341, 5339
BstSFI CTRYAG 3 cut(s) 1466, 1623, 2649
BstSLI GKGCMC 3 cut(s) 48, 867, 3826
BstUI CGCG 3 cut(s) 900, 1060, 2343
BstV1I GCAGC 7 cut(s) 1032, 2635, 3178, 3699, 3736, 4337, 4978
BstXI CCANNNNNNTGG 1 cut(s) 4750
BstZ17I GTATAC 1 cut(s) 1456
Bsu36I CCTNAGG 1 cut(s) 4107
BsuI GTATCC 2 cut(s) 1024, 2454
BsuRI GGCC 8 cut(s) 46, 298, 502, 914, 934, 1094, 3341, 4742
BtgI CCRYGG 3 cut(s) 1124, 1510, 3639
BtgZI GCGATG 1 cut(s) 2324
BtsI GCAGTG 5 cut(s) 4250, 4544, 4728, 4960, 4970
BtuMI TCGCGA 1 cut(s) 2343
BveI ACCTGC 2 cut(s) 280, 949
CaiI CAGNNNCTG 3 cut(s) 941, 1547, 4285
CciI TCATGA 1 cut(s) 2935
CfoI GCGC 2 cut(s) 900, 1749
Cfr13I GGNCC 6 cut(s) 44, 45, 912, 932, 938, 2789
CseI GACGC 5 cut(s) 277, 291, 933, 1450, 5411
Csp6I GTAC 9 cut(s) 334, 817, 1309, 1815, 2527, 2878, 3182, 3620, 3807
CviQI GTAC 9 cut(s) 334, 817, 1309, 1815, 2527, 2878, 3182, 3620, 3807
DraI TTTAAA 2 cut(s) 4737, 4884
DraIII CACNNNGTG 1 cut(s) 3830
Eam1104I CTCTTC 5 cut(s) 359, 428, 669, 4572, 4718
EarI CTCTTC 5 cut(s) 359, 428, 669, 4572, 4718
EciI GGCGGA 1 cut(s) 195
Ecl136II GAGCTC 2 cut(s) 2765, 3239
Eco130I CCWWGG 6 cut(s) 17, 1124, 1205, 1510, 3639, 4052
Eco147I AGGCCT 2 cut(s) 298, 502
Eco24I GRGCYC 8 cut(s) 48, 183, 1299, 1602, 2767, 3241, 5008, 5351
Eco47I GGWCC 2 cut(s) 938, 2789
Eco53kI GAGCTC 2 cut(s) 2765, 3239
Eco57I CTGAAG 4 cut(s) 3945, 4203, 4935, 4967
Eco81I CCTNAGG 1 cut(s) 4107
Eco88I CYCGRG 4 cut(s) 948, 3761, 4518, 4638
Eco91I GGTNACC 1 cut(s) 1572
EcoICRI GAGCTC 2 cut(s) 2765, 3239
EcoNI CCTNNNNNAGG 1 cut(s) 1498
EcoO109I RGGNCCY 3 cut(s) 44, 938, 2789
EcoO65I GGTNACC 1 cut(s) 1572
EcoRI GAATTC 3 cut(s) 835, 3895, 4246
EcoRII CCWGG 8 cut(s) 934, 1026, 1318, 1474, 1499, 1736, 3341, 5339
EcoT14I CCWWGG 6 cut(s) 17, 1124, 1205, 1510, 3639, 4052
EcoT22I ATGCAT 4 cut(s) 1838, 2630, 4220, 4302
EcoT38I GRGCYC 8 cut(s) 48, 183, 1299, 1602, 2767, 3241, 5008, 5351
ErhI CCWWGG 6 cut(s) 17, 1124, 1205, 1510, 3639, 4052
Esp3I CGTCTC 1 cut(s) 951
FaqI GGGAC 1 cut(s) 1301
FauI CCCGC 2 cut(s) 792, 2122
FauNDI CATATG 2 cut(s) 4459, 5114
FbaI TGATCA 3 cut(s) 688, 3016, 4039
FblI GTMKAC 2 cut(s) 1455, 1885
Fnu4HI GCNGC 9 cut(s) 429, 1021, 2649, 3192, 3713, 3750, 4351, 4992, 5475
FriOI GRGCYC 8 cut(s) 48, 183, 1299, 1602, 2767, 3241, 5008, 5351
Fsp4HI GCNGC 9 cut(s) 429, 1021, 2649, 3192, 3713, 3750, 4351, 4992, 5475
GlaI GCGC 2 cut(s) 899, 1748
GluI GCNGC 9 cut(s) 429, 1021, 2649, 3192, 3713, 3750, 4351, 4992, 5475
GsaI CCCAGC 1 cut(s) 4116
GsuI CTGGAG 3 cut(s) 1759, 1928, 5036
HaeII RGCGCY 1 cut(s) 1750
HaeIII GGCC 8 cut(s) 46, 298, 502, 914, 934, 1094, 3341, 4742
HapII CCGG 2 cut(s) 867, 1866
HgaI GACGC 5 cut(s) 277, 291, 933, 1450, 5411
HhaI GCGC 2 cut(s) 900, 1749
Hin1I GRCGYC 1 cut(s) 5403
Hin6I GCGC 2 cut(s) 898, 1747
HinP1I GCGC 2 cut(s) 898, 1747
HincII GTYRAC 4 cut(s) 90, 1886, 2848, 3418
HindII GTYRAC 4 cut(s) 90, 1886, 2848, 3418
HindIII AAGCTT 2 cut(s) 888, 2864
HpaII CCGG 2 cut(s) 867, 1866
Hpy99I CGWCG 3 cut(s) 88, 265, 307
HpyCH4IV ACGT 2 cut(s) 1921, 5197
HpySE526I ACGT 2 cut(s) 1921, 5197
Hsp92I GRCGYC 1 cut(s) 5403
HspAI GCGC 2 cut(s) 898, 1747
Ksp22I TGATCA 3 cut(s) 688, 3016, 4039
Lsp1109I GCAGC 7 cut(s) 1032, 2635, 3178, 3699, 3736, 4337, 4978
MaeII ACGT 2 cut(s) 1921, 5197
MbiI CCGCTC 1 cut(s) 1050
MlyI GAGTC 4 cut(s) 1091, 1941, 3452, 3858
MmeI TCCRAC 4 cut(s) 284, 309, 2496, 4888
Mph1103I ATGCAT 4 cut(s) 1838, 2630, 4220, 4302
MroXI GAANNNNTTC 5 cut(s) 968, 2919, 3930, 4194, 5462
MslI CAYNNNNRTG 6 cut(s) 1900, 2016, 2886, 3287, 4305, 4462
MspCI CTTAAG 1 cut(s) 5078
MspI CCGG 2 cut(s) 867, 1866
MspR9I CCNGG 9 cut(s) 867, 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
Mva1269I GAATGC 3 cut(s) 1937, 2908, 4409
MvaI CCWGG 8 cut(s) 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
MvnI CGCG 3 cut(s) 900, 1060, 2343
NciI CCSGG 1 cut(s) 867
NcoI CCATGG 3 cut(s) 1124, 1510, 3639
NdeI CATATG 2 cut(s) 4459, 5114
NheI GCTAGC 1 cut(s) 1657
NlaIV GGNNCC 8 cut(s) 45, 46, 141, 991, 1203, 1473, 1827, 3637
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 7 cut(s) 1408, 1667, 1673, 3289, 3830, 4017, 5153
NruI TCGCGA 1 cut(s) 2343
NsiI ATGCAT 4 cut(s) 1838, 2630, 4220, 4302
NspI RCATGY 5 cut(s) 536, 1836, 3290, 5154, 5232
NspV TTCGAA 3 cut(s) 314, 527, 4716
PaeR7I CTCGAG 1 cut(s) 3761
PagI TCATGA 1 cut(s) 2935
PaqCI CACCTGC 1 cut(s) 280
PceI AGGCCT 2 cut(s) 298, 502
PciI ACATGT 1 cut(s) 3286
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 80, 311
PctI GAATGC 3 cut(s) 1937, 2908, 4409
PdmI GAANNNNTTC 5 cut(s) 968, 2919, 3930, 4194, 5462
PflFI GACNNNGTC 1 cut(s) 1950
PflMI CCANNNNNTGG 3 cut(s) 1101, 1305, 3201
PkrI GCNGC 9 cut(s) 430, 1022, 2650, 3193, 3714, 3751, 4352, 4993, 5476
PleI GAGTC 4 cut(s) 1090, 1941, 3451, 3857
PpsI GAGTC 4 cut(s) 1090, 1941, 3451, 3857
PpuMI RGGWCCY 2 cut(s) 938, 2789
PscI ACATGT 1 cut(s) 3286
PshAI GACNNNNGTC 2 cut(s) 943, 1081
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 2 cut(s) 2767, 3241
Psp5II RGGWCCY 2 cut(s) 938, 2789
Psp6I CCWGG 8 cut(s) 934, 1026, 1318, 1474, 1499, 1736, 3341, 5339
PspEI GGTNACC 1 cut(s) 1572
PspFI CCCAGC 1 cut(s) 4112
PspGI CCWGG 8 cut(s) 934, 1026, 1318, 1474, 1499, 1736, 3341, 5339
PspN4I GGNNCC 8 cut(s) 45, 46, 141, 991, 1203, 1473, 1827, 3637
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 6 cut(s) 44, 45, 912, 932, 938, 2789
PspPPI RGGWCCY 2 cut(s) 938, 2789
PstI CTGCAG 2 cut(s) 1627, 2653
PstNI CAGNNNCTG 3 cut(s) 941, 1547, 4285
PsyI GACNNNGTC 1 cut(s) 1950
RruI TCGCGA 1 cut(s) 2343
RsaI GTAC 9 cut(s) 335, 818, 1310, 1816, 2528, 2879, 3183, 3621, 3808
RsaNI GTAC 9 cut(s) 334, 817, 1309, 1815, 2527, 2878, 3182, 3620, 3807
RseI CAYNNNNRTG 6 cut(s) 1900, 2016, 2886, 3287, 4305, 4462
SacI GAGCTC 2 cut(s) 2767, 3241
SalI GTCGAC 1 cut(s) 1884
SatI GCNGC 9 cut(s) 429, 1021, 2649, 3192, 3713, 3750, 4351, 4992, 5475
Sau96I GGNCC 6 cut(s) 44, 45, 912, 932, 938, 2789
ScaI AGTACT 1 cut(s) 3183
SchI GAGTC 4 cut(s) 1091, 1941, 3452, 3858
ScrFI CCNGG 9 cut(s) 867, 936, 1028, 1320, 1476, 1501, 1738, 3343, 5341
SfcI CTRYAG 3 cut(s) 1466, 1623, 2649
Sfr274I CTCGAG 1 cut(s) 3761
SfuI TTCGAA 3 cut(s) 314, 527, 4716
SinI GGWCC 2 cut(s) 938, 2789
SlaI CTCGAG 1 cut(s) 3761
SmiMI CAYNNNNRTG 6 cut(s) 1900, 2016, 2886, 3287, 4305, 4462
SmlI CTYRAG 6 cut(s) 2801, 3397, 3485, 3692, 3761, 5078
SmoI CTYRAG 6 cut(s) 2801, 3397, 3485, 3692, 3761, 5078
SseBI AGGCCT 2 cut(s) 298, 502
SspI AATATT 3 cut(s) 1112, 2482, 4176
SstI GAGCTC 2 cut(s) 2767, 3241
StuI AGGCCT 2 cut(s) 298, 502
StyD4I CCNGG 9 cut(s) 865, 934, 1026, 1318, 1474, 1499, 1736, 3341, 5339
StyI CCWWGG 6 cut(s) 17, 1124, 1205, 1510, 3639, 4052
TaiI ACGT 2 cut(s) 1924, 5200
TaqI TCGA 9 cut(s) 314, 527, 1885, 2700, 2761, 3446, 3762, 4716, 4839
TatI WGTACW 5 cut(s) 333, 2526, 3181, 3619, 3806
TauI GCSGC 2 cut(s) 431, 5477
TseFI GTSAC 7 cut(s) 1408, 1667, 1673, 3289, 3830, 4017, 5153
TseI GCWGC 7 cut(s) 1020, 2648, 3191, 3712, 3749, 4350, 4991
Tsp45I GTSAC 7 cut(s) 1408, 1667, 1673, 3289, 3830, 4017, 5153
TspGWI ACGGA 3 cut(s) 1940, 4005, 4955
Tth111I GACNNNGTC 1 cut(s) 1950
Van91I CCANNNNNTGG 3 cut(s) 1101, 1305, 3201
Vha464I CTTAAG 1 cut(s) 5078
VneI GTGCAC 1 cut(s) 3822
VpaK11BI GGWCC 2 cut(s) 938, 2789
XagI CCTNNNNNAGG 1 cut(s) 1498
XapI RAATTY 8 cut(s) 835, 1480, 2586, 2778, 3243, 3895, 4246, 5297
XbaI TCTAGA 2 cut(s) 2824, 4264
XceI RCATGY 5 cut(s) 536, 1836, 3290, 5154, 5232
XcmI CCANNNNNNNNNTGG 1 cut(s) 4363
XhoI CTCGAG 1 cut(s) 3761
XmaJI CCTAGG 1 cut(s) 17
XmiI GTMKAC 2 cut(s) 1455, 1885
XmnI GAANNNNTTC 5 cut(s) 968, 2919, 3930, 4194, 5462
ZrmI AGTACT 1 cut(s) 3183
Zsp2I ATGCAT 4 cut(s) 1838, 2630, 4220, 4302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.