MD06G1081900.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004720
Physical Location & Seq
Reverse (-)
20068477 .. 20088135
19659 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1081900.v1.1.491

Sequence Viewer

Length: 5532 bp
ATGGGGAAAGGAAAACCTAGGGCTGTGGAGAAGGGAGTTGTAGGTCAAACTTTGAGTGTTTCATCATGTGGATCATTGAATATTCCATCTGCACCTGTGTATTACCCTAATGAGGATGAATTTAGAGACCCTTTGGAGTATATTTATAAGATCAGGCCCGAGGCCGAGGCGTATGGGATTTGTAGGATAGTTCCACCGAAGAGCTGGAAACCCCCGTTTGCGTTGGATTTGGATTCGTTTACATTTCCCACAAAGACGCAGGCCATTCACCAGCTGCAGGTGCGCCCTGCATCCTGCGATTCCAAGACTTTTGAGTTGGAGTACAATAGGTTTTTGGAGGACCGTTGTGGGAAGAAGTTGCGGAAGAGGGTGGTTTTCCAAGGGGAGGAGTTGGATTTGTGTAAGTTGTTTAATGCTGTGAAGCGATATGGAGGGTATGATAAGGTTCTGAAGGGGAAGAAGTGGGGGGAGGTTGCTCGTTTTGTGAGGCTGGTGAAAGGGATTTCGGAGTGCTCGAAGCATGTATTGTGTCAACTGTATCGTGACCATTTGTATGATTATGAAAAGTATTATAACAAGCTGAACAGAGAGGGGGCCAGAAGATATAAGAGGGACATGCCTGTGGAGAAGAGGGGTGAACAGAGTTTTGAATGTTCCAGCTCCAAAAGGAGGCGAATCACTAAGGATGGTGGGAAAGTTAAGGTCAGTAAGGGAGAGAAGGAGGATGAGGAGCATGATCAGATATGTGAACAATGCATAAGTGGCTTGCACGGTGAAGTGATGCTTTTGTGTGATAGGTGTAATAAGGGGTGGCACATTTATTGTCTGTCTCCTCCATTGAAGCAGGTTCCGCCAGGGAATTGGTATTGCTTGGAATGCTTGAATTCCGACAAGGATAGTTTTGGCTTCGTGCCTGGTAAGCAGTTCTCGCTGGAAGTTTTTAGGCGTATGGCTAATCGTGCCAAGAGGAAATGGTTTGGGTCAGGATCTGCATCAAGGGTTCAAATAGAGAAAAAGTTTTGGGAAATTGTGGAGGGTTCAGCCGGTGAGGTGGAAGTTATGTATGGCAGTGACTTGGATACTTCCATGTATGGGAGTGGGTTTCCGCGTGTAAATGACCAGAAACCAGAATCAGTTGAGGCCAAGATATGGGATGAATATTGTAGTAGCCCATGGAATCTCAATAACTTACCCAAACTGAAAGGATCAGTGCTTAGAGCTGTTCATCATAACATTGCTGGTGTTATGGTTCCTTGGCTGTATGTTGGGATGCTGTTCTCATCTTTTTGCTGGCATTTTGAAGATCATTGTTTTTATTCAATGAATTATCATCACTGGGGAGAGCCAAAATGTTGGTACAGCGTCCCTGGTAGTGAAGCCAGTGCATTTGAGAAGGTGATGCGCAACAGTCTTCCTGATCTTTTTGATGCACAACCCGATTTACTCTTTCAGCTTGTCACCATGTTAAATCCATCTGTTTTGCAAGAGAATGGAGTTCCAGTATATAGTGTGCTACAGGAGCCTGGGAATTTTGTCATCACCTTCCCCAGATCTTACCATGGAGGCTTTAATCTTGGTTCGAATTGTGCAGAAGCCGTCAATTTTGCTCCAGCGGACTGGCTACCTCATGGTGGGTTTGGAGCAGGGCTGTATCAGCTTTATCACAAAACTGCCGTTCTGTCTCACGAGGAGCTTGTTTGCGTGCTAGCCAAGAGTGACTGTGACAGCAGAGTGTCAACTTATTTAAAGAAAGAATTGACAAGAATCTACAGCAAGGAAAAAACTTGGAGGGAGAGGCTTTGGAGAAAAGGAATCATCGGATCGTCCATTATGTCCTCTCGGAAATGCCCTGAATATGTTGGTACCGAAGAGGATCCTACATGCATTATATGCCAACAGTATCTTTTTCTTTCTGCTGTTGTTTGTCGTTGTAGGCCATCTGCATTTGTATGTCTAGAGCATTGGGAACACCTTTGTGAATGCAAATCCAAGAGACACCGTCTTTTGTATCGTCATTCTCTGGCAGAGTTGCATGAATTGGTGCTTGAAATGGATAAACATTGTTTTGAGGAGACAACACAAAGTAGGACTATAAGAAGGCAAACCTCATTCCCTGATGAACCAACAGCTTTGACAAAAAAGGTGAAAGGGGGCCATGCTACTTTTGCTCAACTTGCAGAACAATGGCTTTTGCGCTCATGCAAGATTTCTCAGAATCTGTTTTCAAGAGATGAGTATGTGTCTTTATTAAAAGAAGCTGAACAGTTTCTTTGGGCTGGCTCAGAGATGAATCCTGTGCGGGAAACGACCAAGAATTTGATCAGATTTCAGATATGGGGTGAAGGTGTAAGAGATTGTCTATCTAAACTTGAAACTTGGTCATCTCATCGTGGAAACAATATAGAGAAAGTTCACTTGGATTATATCAATGAGTTGCTGAGTTTTGATGCGGTGCCCCGTCACGAGACTGGGCATCATATTTTGAAGGGCTACGCCGAAAAGGCAAAGGTGTTGATACAGGAAATTGAATCTGCTATGTCATCATGCCCAAAGCTATCAGAGTTGGAATTATTGTATTCCAGAGCCTGTGAGCTTCCAGTCTGTGTGAAAGAAAGTGAAAATTTACTACAAAGAATTTCATCAGCAAAGGTCTTGATGGAAGGCATGAGAAAATGTATCTCAGAGACACGTACTGCTGCAATTGAGATTGATGTTGTTTACAATTTGAAATCAGAGATTTCAGAGGTCCAAGTTGAACTCCCAGAAACAGATAGGCTTTCGGATCTGTTAAGGAAGGCTGAATCATGTCGTGCCCAATGTGGTGAAATTTTGAAAGGTCCTATTATCCTTGAGGATGTTGAGGTGTTTCTTAAAGAATTAGATGGTTTTACTGTTAACACACCAGAGCTGAAGCTTCTGAGGCAGTACCACACTGATGCTGTTTCTTGGATTTCTCTTCTTAATGCTGTTCTAGTGAACATTCACGAAAGGGAGGATCAAAATGTAGTTAATGAGTTAATGCTCATTTTAACAGATGGAGCATCTTTGAGAATTAAAGTTGATCAGTTGTCTATCGTAGAGTTTGAGTTGAAGAAGGCTCAGTGCAGGGAAAAGGTGCTGAGGATGCGAGATACTAAATTGTCTCTGGACTTCATTCAAGAAGTGATGATGGAGGCAAGAATGTTACATATTGAGGGGGAGAAGTTATTTGTTGATACGTCTGAAGTGTTTGATGCTGCAATGCTGTGGGAGGAAAGAGCGAAATATATCCTTGCACATGAGGCTCAGATATCTGACTTTGAGGATGTTATCAGGAGTTCGGAAGACATACATGTGAATTTATCTTCTCTCCTTGATGTCAAGGATGCACTATCAAAGGCTATGATCTGGTTAAGGAGCTCAAAGCCATTTTTAATGACTTGTTTTCCTTTGGTATCTGTTTCAAGTTCTTTGCTGAATGTCGACACCTTGAAGGAATTAGTTTCCGAGTCAAAGCATCTGAAGGTTTCTTTGAAAGAAATAAGGATGCTTGAGACTGTTTTGATGAACTGTAAGGAATGGGAGAATGATGCTTGCTCTCTACTGCAAGATACAAGGTTCCTATTTGATATTAGGATCATTGGTGATGGAACAAGAGATGGTCTTCTTTCCAAGATTGAACATCTAATTGCAAGAATCGGATCCTTGGAAAGCACTGGTTTATCTCTTTCTTTTGACTTTGGCGAGCTTGTGAAACTTAAGGAGGCTTGTTCTGTGCTGCAATGGTGTAAGAAGGCTCTTTCTTTCTGCTCTGGAGTTCCTCCTCTTGAGGATGTTGAAGATTTGGTGAGTGCTGCAGGAAATTTACATGGTACATATGTCCCTAGTGCATTGTGGGGCTTGTTGGTTGAAGGGCTCAAATGGCTTGAGCATGCTACAAAGGTTATTTCTGCACCATGCAACTCTGAAAGATGCACGTTAAGTGAAGCTGAAGAAGTACTAGCTAAATCTCAGAGTATTAGTGTCTCCTTCCCATTGATGGTTGATCAACTTGAATGTGCTATTCAGAAACACAAATCCTGGCTTGAACAAGTGCATCAACTTTTCAGCCTAAGGCCAGAAGAACGATCTTGGTCTCTAACATTGCAGTTAAAGGAACTTGGAGTTTCTACTGCTTTCAGTTGCACAGAACTAGATTTGATTGTATCTGAAGTTGAAAGGGTTGAGAGTTGGAAACGACAGTGCATGGATATTGTCAGGGTAGTAGTTGAAGATGATAATTCATTGCTTGGCGGTTTAGAAAAGATGGGACAGACTCTAGATCGATGTATGCGGATATATGAGAAGCCACATGGCTTGAAAGAGAGTAGTTCCTTTGCTTGCTGCTCCAGTGGTTCTCTGGATCAAGAATTTCTTACTTGTTCCTCATGCAAGGACTGCTACCATTTGGGGTGTCTAGGGTCTTCCATAATAGATGGAAAACATGCAGAATATGTGTGCCCAGGTTGCCGACATCTAGTATCTGGAACAACTTCTCAAAATGGACGCAGTTCTCCGATATTTGGAGGGGTGCGGCCAGAACTGAAAAAAATTATTGAACATCTATCCGGTGGCGAAGACTTCTGTGTTCGTATTGAAGAAAGAGACGTGCTGAAAAAAATTTTGAAGCAAGCTCTGGCATGCAAATCACGGTTGACAGAAATAGTGGATTTTACGTTAGCTTATTCTGATAAAGATCTCAGTGTCATCTCTGAAAAGCTAACCACGGCTTTAAAGGCCAGAGAAATTCAAGGTGTTCATGATCATGAAGGGGATAGCAACCTTATGTTGGCCTTATCAAGATACTCATGGAAAGTCAGAGTCGACAGTTTATTGGAGGGTTCACAAAAGCCCACAATCCAACAGATTCAGCAGCAGTTAAAAGAGGGAGCTGCCTTAAACATTCCACCCGAAGATTACTATTGGCAGAAACTTACAGAAGTGAGGATTATTGGTTTGCAGTGGGCAGATACTGCCAAGACGGTTGTTGCCGATTCTGGAGCTGTCCCCTTGGATAAAATTTTTGAACTTGTTTCGGTGGGTGAAAATTTGCCTGTTCGTGTAGAGAAGGAACTTAAGTTGTTGAAAGCTCGGAGTATGCTTTATTGCATATGTCGAAAGCCGTATGATCAGCGAGCAATGATTGCGTGTGACCAATGTGATGAGTGGTATCACTTTGATTGCATAAAACTGCGTTCCACGCCAAAGACTTACATATGCCCTGCATGTCAGCCTCTGGCAGAAGAGTTGTCTGCTGAACCCGCGGTGGATCAAGAGAGATGTACCGATATGAAATCTGTAGAGCCCAAAACGCCCTCTCCTACGCACACAAAGCGCCGGACGAACCCAAAGAAGGCAGAGTCCGTTATAGCACAAAAGATGCTTGCAATTAGAGAGCCCAACAATAGTTTTAGGTGTTCTAGTGGGATAGAACGTTTATGGTGGCGAAATCGGAAACCTTTCAGAAGGGCGGCTAAAAGACGCGCCGAGCTTGAAAGTCTCTCTTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1844

Amino Acids

210.0

Weight (kDa)

6.52

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 32 - 65 3.5e-15 jmjN domain
ARID PF01388 102 - 188 8.7e-13 ARID/BRIGHT DNA binding domain
PHD PF00628 249 - 293 4.3e-08 PHD-finger
JmjC PF02373 419 - 535 2.4e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 628 - 680 1e-14 C5HC2 zinc finger
PLU-1 PF08429 950 - 1133 3.4e-14 PLU-1-like protein
PLU-1 PF08429 1149 - 1407 1.8e-16 PLU-1-like protein
PHD PF00628 1709 - 1749 2e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 147, 573
AarI CACCTGC 1 cut(s) 268
Acc16I TGCGCA 1 cut(s) 1403
Acc36I ACCTGC 2 cut(s) 268, 835
Acc65I GGTACC 1 cut(s) 1862
AccB1I GGYRCC 2 cut(s) 1862, 2452
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccI GTMKAC 2 cut(s) 3462, 4842
AccII CGCG 3 cut(s) 1108, 5282, 5502
AclI AACGTT 1 cut(s) 5452
AcoI YGGCCR 1 cut(s) 4553
AcuI CTGAAG 6 cut(s) 470, 2930, 3243, 3521, 3990, 4209
AfaI GTAC 8 cut(s) 323, 1358, 1864, 2692, 2927, 3853, 3978, 5302
AflII CTTAAG 2 cut(s) 3737, 5093
AflIII ACRYGT 2 cut(s) 2687, 3331
AjiI CACGTC 1 cut(s) 4627
AjnI CCWGG 6 cut(s) 853, 913, 1366, 1522, 4058, 4480
AjuI GAANNNNNNNTTGG 2 cut(s) 2399, 2431
AleI CACNNNNGTG 1 cut(s) 1974
Alw21I GWGCWC 2 cut(s) 515, 3401
AlwNI CAGNNNCTG 5 cut(s) 989, 2218, 2586, 4991, 5254
Ama87I CYCGRG 1 cut(s) 158
ApeKI GCWGC 8 cut(s) 274, 2696, 3236, 3757, 3833, 4362, 4891, 4910
ArsI GACNNNNNNTTYG 2 cut(s) 2299, 2331
Asp700I GAANNNNTTC 3 cut(s) 2265, 4510, 5477
Asp718I GGTACC 1 cut(s) 1862
AspA2I CCTAGG 1 cut(s) 17
AspLEI GCGC 5 cut(s) 285, 1404, 2196, 5355, 5504
AspS9I GGNCC 6 cut(s) 156, 340, 594, 2152, 2746, 2837
AsuII TTCGAA 1 cut(s) 1580
AsuNHI GCTAGC 1 cut(s) 1705
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 3 cut(s) 340, 2746, 2837
AvrII CCTAGG 1 cut(s) 17
AxyI CCTNAGG 1 cut(s) 4091
BaeGI GKGCMC 3 cut(s) 2457, 2815, 4481
BaeI ACNNNNGTAYC 2 cut(s) 3843, 3876
BamHI GGATCC 2 cut(s) 1873, 3680
BanI GGYRCC 2 cut(s) 1862, 2452
BanII GRGCYC 4 cut(s) 3401, 3897, 5325, 5418
BauI CACGAG 2 cut(s) 1685, 2462
BbsI GAAGAC 5 cut(s) 1403, 3330, 3635, 4434, 4602
Bbv12I GWGCWC 2 cut(s) 515, 3401
BbvCI CCTCAGC 1 cut(s) 3119
BbvI GCAGC 8 cut(s) 261, 2683, 3223, 3744, 3820, 4349, 4897, 4903
BceAI ACGGC 4 cut(s) 1580, 1658, 4761, 5125
BciT130I CCWGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
BciVI GTATCC 1 cut(s) 1072
BclI TGATCA 6 cut(s) 736, 2319, 3061, 4024, 4780, 5146
BfmI CTRYAG 5 cut(s) 275, 1514, 1768, 3834, 5316
BfoI RGCGCY 1 cut(s) 5356
BfrI CTTAAG 2 cut(s) 3737, 5093
BfuAI ACCTGC 2 cut(s) 268, 835
BfuI GTATCC 1 cut(s) 1072
BglI GCCNNNNNGGC 1 cut(s) 2501
BglII AGATCT 2 cut(s) 1550, 4714
BlnI CCTAGG 1 cut(s) 17
BmcAI AGTACT 1 cut(s) 3978
Bme1390I CCNGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
Bme18I GGWCC 3 cut(s) 340, 2746, 2837
BmeT110I CYCGRG 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 4627
BmgT120I GGNCC 6 cut(s) 156, 340, 594, 2152, 2746, 2837
BmrFI CCNGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
BmrI ACTGGG 2 cut(s) 1345, 2478
BmtI GCTAGC 1 cut(s) 1709
BmuI ACTGGG 2 cut(s) 1345, 2478
BpiI GAAGAC 5 cut(s) 1403, 3330, 3635, 4434, 4602
BpmI CTGGAG 4 cut(s) 1593, 3813, 4351, 5037
Bpu10I CCTNAGC 1 cut(s) 3119
Bpu14I TTCGAA 1 cut(s) 1580
BpuEI CTTGAG 4 cut(s) 2870, 3551, 3827, 3926
Bsa29I ATCGAT 1 cut(s) 4303
BsaAI YACGTR 1 cut(s) 2690
BsaBI GATNNNNATC 2 cut(s) 991, 4713
BsaI GGTCTC 2 cut(s) 120, 4119
BsaWI WCCGGW 1 cut(s) 4586
BsaXI ACNNNNNCTCC 4 cut(s) 1485, 1515, 5320, 5350
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 8 cut(s) 1340, 1380, 1499, 1622, 2473, 2597, 3700, 4368
Bse21I CCTNAGG 1 cut(s) 4091
Bse3DI GCAATG 6 cut(s) 1233, 3246, 3767, 4121, 4262, 5163
Bse8I GATNNNNATC 2 cut(s) 991, 4713
BseBI CCWGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
BseCI ATCGAT 1 cut(s) 4303
BseJI GATNNNNATC 2 cut(s) 991, 4713
BseMI GCAATG 6 cut(s) 1233, 3246, 3767, 4121, 4262, 5163
BseNI ACTGG 8 cut(s) 1340, 1380, 1499, 1622, 2473, 2597, 3700, 4368
BseRI GAGGAG 6 cut(s) 401, 743, 822, 1703, 2084, 3792
BseSI GKGCMC 3 cut(s) 2457, 2815, 4481
BseXI GCAGC 8 cut(s) 261, 2683, 3223, 3744, 3820, 4349, 4897, 4903
BsgI GTGCAG 4 cut(s) 75, 1608, 3124, 3915
Bsh1236I CGCG 3 cut(s) 1108, 5282, 5502
BshNI GGYRCC 2 cut(s) 1862, 2452
BshVI ATCGAT 1 cut(s) 4303
BsiHKAI GWGCWC 2 cut(s) 515, 3401
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 3 cut(s) 1044, 4587, 5356
BslFI GGGAC 5 cut(s) 626, 1349, 3845, 4302, 5009
BsmBI CGTCTC 1 cut(s) 4617
BsmFI GGGAC 5 cut(s) 626, 1349, 3845, 4302, 5009
BsmI GAATGC 2 cut(s) 881, 1985
Bso31I GGTCTC 2 cut(s) 120, 4119
BsoBI CYCGRG 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 1580
Bsp1286I GDGCHC 8 cut(s) 515, 2457, 2815, 3401, 3897, 4481, 5325, 5418
Bsp19I CCATGG 2 cut(s) 1172, 1558
BspDI ATCGAT 1 cut(s) 4303
BspFNI CGCG 3 cut(s) 1108, 5282, 5502
BspHI TCATGA 2 cut(s) 4777, 4783
BspMAI CTGCAG 2 cut(s) 279, 3838
BspMI ACCTGC 2 cut(s) 268, 835
BspOI GCTAGC 1 cut(s) 1709
BspQI GCTCTTC 1 cut(s) 194
BspT104I TTCGAA 1 cut(s) 1580
BspT107I GGYRCC 2 cut(s) 1862, 2452
BspTI CTTAAG 2 cut(s) 3737, 5093
BspTNI GGTCTC 2 cut(s) 120, 4119
BsrDI GCAATG 6 cut(s) 1233, 3246, 3767, 4121, 4262, 5163
BsrFI RCCGGY 1 cut(s) 1043
BsrI ACTGG 8 cut(s) 1340, 1380, 1499, 1622, 2473, 2597, 3700, 4368
BssAI RCCGGY 1 cut(s) 1043
BssSI CACGAG 2 cut(s) 1685, 2462
BssT1I CCWWGG 7 cut(s) 17, 379, 1172, 1253, 1558, 3684, 5028
Bst2BI CACGAG 2 cut(s) 1685, 2462
Bst2UI CCWGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
Bst6I CTCTTC 6 cut(s) 194, 359, 623, 1863, 2961, 5256
BstAFI CTTAAG 2 cut(s) 3737, 5093
BstAPI GCANNNNNTGC 4 cut(s) 1890, 4416, 4991, 5162
BstBAI YACGTR 1 cut(s) 2690
BstBI TTCGAA 1 cut(s) 1580
BstDSI CCRYGG 4 cut(s) 1172, 1558, 4743, 5280
BstFNI CGCG 3 cut(s) 1108, 5282, 5502
BstH2I RGCGCY 1 cut(s) 5356
BstHHI GCGC 5 cut(s) 285, 1404, 2196, 5355, 5504
BstNI CCWGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
BstNSI RCATGY 8 cut(s) 524, 619, 1884, 3335, 3914, 4466, 4662, 5247
BstSCI CCNGG 6 cut(s) 853, 913, 1366, 1522, 4058, 4480
BstSFI CTRYAG 5 cut(s) 275, 1514, 1768, 3834, 5316
BstSLI GKGCMC 3 cut(s) 2457, 2815, 4481
BstUI CGCG 3 cut(s) 1108, 5282, 5502
BstV1I GCAGC 8 cut(s) 261, 2683, 3223, 3744, 3820, 4349, 4897, 4903
BstV2I GAAGAC 5 cut(s) 1403, 3330, 3635, 4434, 4602
BstX2I RGATCY 6 cut(s) 986, 1550, 1873, 2782, 3680, 4714
BstXI CCANNNNNNTGG 3 cut(s) 861, 1353, 1617
BstYI RGATCY 6 cut(s) 986, 1550, 1873, 2782, 3680, 4714
Bsu15I ATCGAT 1 cut(s) 4303
Bsu36I CCTNAGG 1 cut(s) 4091
BsuI GTATCC 1 cut(s) 1072
BsuTUI ATCGAT 1 cut(s) 4303
BtgI CCRYGG 4 cut(s) 1172, 1558, 4743, 5280
BtrI CACGTC 1 cut(s) 4627
BtsI GCAGTG 2 cut(s) 1075, 4985
BveI ACCTGC 2 cut(s) 268, 835
CaiI CAGNNNCTG 5 cut(s) 989, 2218, 2586, 4991, 5254
CciI TCATGA 2 cut(s) 4777, 4783
CfoI GCGC 5 cut(s) 285, 1404, 2196, 5355, 5504
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 6 cut(s) 156, 340, 594, 2152, 2746, 2837
Cfr42I CCGCGG 1 cut(s) 5283
ClaI ATCGAT 1 cut(s) 4303
CseI GACGC 4 cut(s) 265, 1351, 4533, 5508
Csp6I GTAC 8 cut(s) 322, 1357, 1863, 2691, 2926, 3852, 3977, 5301
CspCI CAANNNNNGTGG 2 cut(s) 4317, 4352
CviQI GTAC 8 cut(s) 322, 1357, 1863, 2691, 2926, 3852, 3977, 5301
DraI TTTAAA 2 cut(s) 1746, 4752
EaeI YGGCCR 1 cut(s) 4553
Eam1104I CTCTTC 6 cut(s) 194, 359, 623, 1863, 2961, 5256
EarI CTCTTC 6 cut(s) 194, 359, 623, 1863, 2961, 5256
EciI GGCGGA 1 cut(s) 840
Ecl136II GAGCTC 1 cut(s) 3399
Eco130I CCWWGG 7 cut(s) 17, 379, 1172, 1253, 1558, 3684, 5028
Eco24I GRGCYC 4 cut(s) 3401, 3897, 5325, 5418
Eco31I GGTCTC 2 cut(s) 120, 4119
Eco32I GATATC 1 cut(s) 3291
Eco47I GGWCC 3 cut(s) 340, 2746, 2837
Eco53kI GAGCTC 1 cut(s) 3399
Eco57I CTGAAG 6 cut(s) 470, 2930, 3243, 3521, 3990, 4209
Eco81I CCTNAGG 1 cut(s) 4091
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 3399
EcoO109I RGGNCCY 1 cut(s) 2837
EcoRI GAATTC 1 cut(s) 883
EcoRII CCWGG 6 cut(s) 853, 913, 1366, 1522, 4058, 4480
EcoRV GATATC 1 cut(s) 3291
EcoT14I CCWWGG 7 cut(s) 17, 379, 1172, 1253, 1558, 3684, 5028
EcoT22I ATGCAT 2 cut(s) 758, 1886
EcoT38I GRGCYC 4 cut(s) 3401, 3897, 5325, 5418
ErhI CCWWGG 7 cut(s) 17, 379, 1172, 1253, 1558, 3684, 5028
Esp3I CGTCTC 1 cut(s) 4617
FalI AAGNNNNNCTT 5 cut(s) 768, 800, 4377, 4409, 5506
FaqI GGGAC 5 cut(s) 626, 1349, 3845, 4302, 5009
FauI CCCGC 2 cut(s) 2292, 5287
FauNDI CATATG 3 cut(s) 3856, 5129, 5234
FbaI TGATCA 6 cut(s) 736, 2319, 3061, 4024, 4780, 5146
FblI GTMKAC 2 cut(s) 3462, 4842
FriOI GRGCYC 4 cut(s) 3401, 3897, 5325, 5418
FspI TGCGCA 1 cut(s) 1403
GlaI GCGC 5 cut(s) 284, 1403, 2195, 5354, 5503
GsuI CTGGAG 4 cut(s) 1593, 3813, 4351, 5037
HaeII RGCGCY 1 cut(s) 5356
HapII CCGG 3 cut(s) 1044, 4587, 5356
HgaI GACGC 4 cut(s) 265, 1351, 4533, 5508
HhaI GCGC 5 cut(s) 285, 1404, 2196, 5355, 5504
Hin6I GCGC 5 cut(s) 283, 1402, 2194, 5353, 5502
HinP1I GCGC 5 cut(s) 283, 1402, 2194, 5353, 5502
HincII GTYRAC 6 cut(s) 533, 1737, 2896, 3463, 4674, 4843
HindII GTYRAC 6 cut(s) 533, 1737, 2896, 3463, 4674, 4843
HindIII AAGCTT 1 cut(s) 2912
HpaI GTTAAC 1 cut(s) 2896
HpaII CCGG 3 cut(s) 1044, 4587, 5356
HpyCH4IV ACGT 6 cut(s) 2689, 3218, 3956, 4626, 4694, 5452
HpySE526I ACGT 6 cut(s) 2689, 3218, 3956, 4626, 4694, 5452
HspAI GCGC 5 cut(s) 283, 1402, 2194, 5353, 5502
KpnI GGTACC 1 cut(s) 1866
Ksp22I TGATCA 6 cut(s) 736, 2319, 3061, 4024, 4780, 5146
KspAI GTTAAC 1 cut(s) 2896
KspI CCGCGG 1 cut(s) 5283
LguI GCTCTTC 1 cut(s) 194
Lsp1109I GCAGC 8 cut(s) 261, 2683, 3223, 3744, 3820, 4349, 4897, 4903
MaeII ACGT 6 cut(s) 2689, 3218, 3956, 4626, 4694, 5452
MaeIII GTNAC 8 cut(s) 542, 1070, 1456, 1715, 1721, 2459, 3183, 5168
MfeI CAATTG 1 cut(s) 2700
MflI RGATCY 6 cut(s) 986, 1550, 1873, 2782, 3680, 4714
MhlI GDGCHC 8 cut(s) 515, 2457, 2815, 3401, 3897, 4481, 5325, 5418
MlyI GAGTC 4 cut(s) 3497, 4288, 4848, 5387
MmeI TCCRAC 7 cut(s) 204, 297, 372, 912, 2544, 4190, 4903
Mph1103I ATGCAT 2 cut(s) 758, 1886
MroXI GAANNNNTTC 3 cut(s) 2265, 4510, 5477
MslI CAYNNNNRTG 7 cut(s) 552, 620, 1948, 1974, 2934, 3332, 4782
MspA1I CMGCKG 3 cut(s) 274, 1613, 5282
MspCI CTTAAG 2 cut(s) 3737, 5093
MspI CCGG 3 cut(s) 1044, 4587, 5356
MspR9I CCNGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
MunI CAATTG 1 cut(s) 2700
Mva1269I GAATGC 2 cut(s) 881, 1985
MvaI CCWGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
MvnI CGCG 3 cut(s) 1108, 5282, 5502
NcoI CCATGG 2 cut(s) 1172, 1558
NdeI CATATG 3 cut(s) 3856, 5129, 5234
NheI GCTAGC 1 cut(s) 1705
NmeAIII GCCGAG 2 cut(s) 190, 5530
NmuCI GTSAC 7 cut(s) 542, 1070, 1456, 1715, 1721, 2459, 5168
NsbI TGCGCA 1 cut(s) 1403
NsiI ATGCAT 2 cut(s) 758, 1886
NspI RCATGY 8 cut(s) 524, 619, 1884, 3335, 3914, 4466, 4662, 5247
NspV TTCGAA 1 cut(s) 1580
OliI CACNNNNGTG 1 cut(s) 1974
PaeI GCATGC 2 cut(s) 3914, 4662
PagI TCATGA 2 cut(s) 4777, 4783
PaqCI CACCTGC 1 cut(s) 268
PciI ACATGT 1 cut(s) 3331
PciSI GCTCTTC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PctI GAATGC 2 cut(s) 881, 1985
PdmI GAANNNNTTC 3 cut(s) 2265, 4510, 5477
PflFI GACNNNGTC 1 cut(s) 1998
PflMI CCANNNNNTGG 1 cut(s) 1149
PleI GAGTC 4 cut(s) 3496, 4288, 4847, 5386
PpsI GAGTC 4 cut(s) 3496, 4288, 4847, 5386
Ppu21I YACGTR 1 cut(s) 2690
PpuMI RGGWCCY 1 cut(s) 2837
PscI ACATGT 1 cut(s) 3331
PsiI TTATAA 2 cut(s) 147, 573
Psp124BI GAGCTC 1 cut(s) 3401
Psp1406I AACGTT 1 cut(s) 5452
Psp5II RGGWCCY 1 cut(s) 2837
Psp6I CCWGG 6 cut(s) 853, 913, 1366, 1522, 4058, 4480
PspGI CCWGG 6 cut(s) 853, 913, 1366, 1522, 4058, 4480
PspPI GGNCC 6 cut(s) 156, 340, 594, 2152, 2746, 2837
PspPPI RGGWCCY 1 cut(s) 2837
PstI CTGCAG 2 cut(s) 279, 3838
PstNI CAGNNNCTG 5 cut(s) 989, 2218, 2586, 4991, 5254
PsuI RGATCY 6 cut(s) 986, 1550, 1873, 2782, 3680, 4714
PsyI GACNNNGTC 1 cut(s) 1998
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 8 cut(s) 323, 1358, 1864, 2692, 2927, 3853, 3978, 5302
RsaNI GTAC 8 cut(s) 322, 1357, 1863, 2691, 2926, 3852, 3977, 5301
RseI CAYNNNNRTG 7 cut(s) 552, 620, 1948, 1974, 2934, 3332, 4782
SacI GAGCTC 1 cut(s) 3401
SacII CCGCGG 1 cut(s) 5283
SalI GTCGAC 2 cut(s) 3461, 4841
SapI GCTCTTC 1 cut(s) 194
Sau96I GGNCC 6 cut(s) 156, 340, 594, 2152, 2746, 2837
ScaI AGTACT 1 cut(s) 3978
SchI GAGTC 4 cut(s) 3497, 4288, 4848, 5387
ScrFI CCNGG 6 cut(s) 855, 915, 1368, 1524, 4060, 4482
SduI GDGCHC 8 cut(s) 515, 2457, 2815, 3401, 3897, 4481, 5325, 5418
SfcI CTRYAG 5 cut(s) 275, 1514, 1768, 3834, 5316
Sfr303I CCGCGG 1 cut(s) 5283
SfuI TTCGAA 1 cut(s) 1580
SgrBI CCGCGG 1 cut(s) 5283
SinI GGWCC 3 cut(s) 340, 2746, 2837
SmiMI CAYNNNNRTG 7 cut(s) 552, 620, 1948, 1974, 2934, 3332, 4782
SmlI CTYRAG 6 cut(s) 2849, 3530, 3737, 3806, 3905, 5093
SmoI CTYRAG 6 cut(s) 2849, 3530, 3737, 3806, 3905, 5093
SphI GCATGC 2 cut(s) 3914, 4662
SspI AATATT 2 cut(s) 82, 1160
SstI GAGCTC 1 cut(s) 3401
StyD4I CCNGG 6 cut(s) 853, 913, 1366, 1522, 4058, 4480
StyI CCWWGG 7 cut(s) 17, 379, 1172, 1253, 1558, 3684, 5028
TaiI ACGT 6 cut(s) 2692, 3221, 3959, 4629, 4697, 5455
TaqI TCGA 6 cut(s) 515, 1580, 3462, 4303, 4842, 5134
TatI WGTACW 2 cut(s) 321, 3976
TauI GCSGC 2 cut(s) 4555, 5492
TseFI GTSAC 7 cut(s) 542, 1070, 1456, 1715, 1721, 2459, 5168
TseI GCWGC 8 cut(s) 274, 2696, 3236, 3757, 3833, 4362, 4891, 4910
Tsp45I GTSAC 7 cut(s) 542, 1070, 1456, 1715, 1721, 2459, 5168
TspGWI ACGGA 1 cut(s) 5371
Tth111I GACNNNGTC 1 cut(s) 1998
Van91I CCANNNNNTGG 1 cut(s) 1149
Vha464I CTTAAG 2 cut(s) 3737, 5093
VpaK11BI GGWCC 3 cut(s) 340, 2746, 2837
XbaI TCTAGA 2 cut(s) 1954, 4297
XceI RCATGY 8 cut(s) 524, 619, 1884, 3335, 3914, 4466, 4662, 5247
XcmI CCANNNNNNNNNTGG 2 cut(s) 201, 4375
XmaJI CCTAGG 1 cut(s) 17
XmiI GTMKAC 2 cut(s) 3462, 4842
XmnI GAANNNNTTC 3 cut(s) 2265, 4510, 5477
ZrmI AGTACT 1 cut(s) 3978
Zsp2I ATGCAT 2 cut(s) 758, 1886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.