pycom14g09970

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004720
Physical Location & Seq
Reverse (-)
12286088 .. 12295705
9618 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g09970.6

Sequence Viewer

Length: 3714 bp
ATGTCCTCTCGGACATGCCCTGAGTATGTTGGTACCGAAGAGGATCCTACATGCATTATATGCCAACAGTATCTTTTTCTTTCTGCTGTTGTTTGTCGTTGTAGGCCATCTGCATTTGTATGTCTGGAGCATTGGGAACACCTTTGCGAATGCAAATCCAGAAGACTCCGTCTTTTATATCGTTATTCTCTGGCAGAGTTGCATGAATTGGTCCTTGAAATGGATAAACATGGTTTTGAGGAGACAACACAAAGTAGGACTATAAGAAGGCAAATCTCATGCACTGATGAACCAACGGCTTTGACAAAAAAGGTCAAAGGGGGCCATGCCACTTTTGCTCAACTTGCAGAACAATGGCTTTTGCGCTCGTGCAAGATTTCTCAGAATCCATTTTCAAGAGATGAGTATGTGTCTTTATTAAAAGAAGCTGAACAGTTTCTTTGGGCTGGCTCAGAGATGAATCCTGTGCGGGAAACAACCAAGAATTTGATCAGATTTCAGAAATGGGCTGAAGGTGTAAGAGATTGTCTATCTAAACTTGAAACTTGGTCATCTCGTAGTGGAAACAGTATCGAGAAAGTTCACTTGGATTATATCAATGAATTGCTGAGTTTTGATGTGGTGCCCTGTTACGAGACTGGGCATCATAATTTGAAGGGTTATGCAGAAAAGGCAAAAATGTTGATACAGGAAATTGAATCTGCTATGTCATCATGCCTAAAGATATCGGAGTTGAAATTGTTGTATTCCAGAGCCTGTGAGCTTCCAGTCTATGTGAAAGAAAGTGAAAATTTACTACAAAAAATTTCATCTGCAGAGGTCTTGATGGAAGGCATAAGAAAATGTATCTCAGAGACACGTACTGCAGCAATTGGGATTGATGTTGTTTACAATTTGAATTCAGAGATTTCAGAGCTCCAAGTTGAACTCCCAGACATGGATAGGCTTTCAGATCTATTAAGGACAGCTGAATCATGGCGTGCTCGATGTGGTGAAATTTTGAAAGGTCCTATTAGCCTTGAGGATGTTGAGGCGCTTCTGAAACAATTGGATGGTTTTACTGTTAACACACCAGAGCTGAAGCTTCTGAAGCAGTACCACATTGATACAGTTTCTTGGATTTCTCGTCTTAATGCTGTTCTTGTGAACATTCACGAAAGGGAGGATCAAAATAATGTAGTTAATGAGTTAATGCTCATATTAACAGATGGAGCATCTTTGAAAATTAAAGTTGATCAGTTGTCTATCGTAGAGTTTGAGTTGAAGAAGGCTCAGTGCAGGGAAAAGGCGCTGAGGATGCGAGATACTAAATTGTCTCTGGACTTCATTCAAGAAGTGATGATGGAGGCAAGAATGTTACATGTTGAGGGGGAGAAGTTATTCGTTGATATGTCTGAAGTGTTAGCTGTTGCTATGCTATGGGAGGAAAGAGCGAAATATATCCTTGCACACAAGGCTCAGATATCTGACTTTGAGGATGTTATCAGGAGTTCGGAAGACATACATGTGCATTTATCTTCTCTCCATGATGTCAAGGATGCACTATCAAAGGCTAAGATCTGGTTGAGTAGGTCAAAGCCATTTTTAATGACTTCTCCTTTGGTATCTGTTTCAAGTCCTTTGCTGAATGTCGACACCTTGAAGGAATTAGTTTCTGAGTCAAAGCCTCTGAAGGTTTCTTTGAAAGAAATAAGGATGCTTGAGACTGTTTTGATGAACTGTAAGGAATGGGAGAATGACGCTTGCTGTCTACTGCAAGATACAAGGTGCCTATTTGATATGAGGATCATTGGAGATGGAACAAGAGATGGTCTTCTTTCTAAGATTGAACATCTAATTGCAAGAATCAAATCCATGGGAAGCACTGGTTTATCTCTTTCTTTTGACTTTGGCGAGCTTGTGAAACTTAAGGAGGCTTGTTCTGTGCTGCAATGGTGTAAGAAGGCTCTTTCTTTCAGCATTGGAGTAACTCCCCTTGAGGATGTTGAAAATTTGGCGAGTGCTGCAGAAAATTTATGTGGTACATTTGCCCCTAGTGCATTGTGGGGCTCGTTGGTTGAAGGGCTGAAATGGCTTGAGCATGCTACAAAGGTTATTTCTGCATCATGCAATTCTAAAAGATGCACTTTAGGTGAAGCTGAAGAAGTTCTTTCTAAATCTCAGAGTATCAGTGTCTCCTTCCCATTGATGGTTGATCAACTTGAATGTGCTATTCAGAAACACAAGTCCTGGCTTGAGCAAGTGCATCAACTTTTCAGCCTAAGGCCAGGAGAACGATCTTGGTCTCTAACATTACAGCTAAAGGAACTTGGAGTTTCTGCTGCTTTCAGTTGCACAGAACTAGATTTGATTGTATCTGAAGTTGAAAGGGTTGAGAGTTGGAAACAACAGTGCATGGATATTTTCAGGATAGTAGTTGGGGATGATAATTCATTGCTTGGCAGTTTAGAAAAGATAGGACAGACTCTGAAGAGATGTATACACATATATGAGAAACCACATGGCTTGAAAGAGAGTAGTTCTTTTGCTTGCTGCTCCAGTGGTTCTCTGGATCAGGAATTTCTTACTTGTTCTTCATGCAAGGACTGCTACCATTTGAGGTGTCTAGGGTCTTCCATGGTAGGTGGAAAACATGCAGAATATGAATGCCCATGTTGCCGATATCTGGTATCTGGAACTTCTTCTCCAAGTGGGCTCAGTACTCTGAAATTTGGTGGGATGCGTCCAGAACTTCAAAAAATTATTGAACATTTATCCGGTGAAGAAGACTTCTGTGTTTGTATTAAAGAAAGAGATGTACTGAAAGAAGTTTTGAAGCAAGCTCAAGCATGCAAATCTCGCTTGACAGAATTAGTGGATTTTGCATTAGCTTATTCTGATAAGGATCTCAGTGTGCTCGTTGAAAAACTAACAACAGCTTTAAAGGCCAGAGAAATGCAGGGTGTACATGATCATGAAGGTGATATCAACCTTATGTTGGCTTTATCAAGATACTCATGGAAAGTCAGAGTCAATAGGTTATTGGAGGGTTCACAAAAGCCCACAATCCAACAGGTTCAGCAGCAGTTAAAAGAGGGAGCTGCCTTGAACATTCTGCCTGCGGATTATTATTGGCAGAAACTTACCGAAGTGAGGATTATTGGTTTGCAGTGGGCAGATACTGCTAAGAAGGTTGCTGCCGATTCTGGGGCTCTCCCCTTGGATAAAGTTTTTGAACTTGTTTCGGAGGGTGAAAATTTGCCTGTTCGTCTAGAGAGGGAACTTAAGTTGTTGAAAGCTCGGAGTATGCTTTATTGCATTTGCCGAAAGCCGTATGATCAACGAGCAATGATTGCGTGTGACCAATGTAATGAGTGGTATCACTTTGATTGCATTAAATTACGTTCCACACCAAAGGTTTACATATGCCCTGCATGTCAGCCTCTAGGAGAAGAGTGTTGTGAACCCGTGGTGGATCAAGAGAGATGTAGTGATGCGAAATCTGTAGAGCCCAAAACGCCCTCTCCTACGCATGCAAAGTGCAGGACGAACCCAAAGAAGGCGGAGTCCAATCTAGCACAAAAGATTCTTGCAATTACAGACCCCAACAATGTTTATAGGTGTTCTAGCGGGATCGAACGTTTATGGTGGCGAAATCGGAAACCTTTCAGAAGGGCGGCTAAACGACGTGCCAAGCTTGATAGTCTCTCTGTGTTTTCTCATTTGGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0001046 GO:0001047 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007623 GO:0008150 GO:0008152 GO:0008214 GO:0008270 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031057 GO:0031063 GO:0031064 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031490 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032922 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034647 GO:0034648 GO:0034720 GO:0034721 GO:0035064 GO:0036211 GO:0042393 GO:0043086 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043565 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051090 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0060255 GO:0065007 GO:0065009 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090311 GO:0097159 GO:0140030 GO:0140034 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901725 GO:1901726 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990837 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ARID JmjC JmjN PHD PLU-1 zf-C5HC2

Protein Analysis

1238

Amino Acids

140.13

Weight (kDa)

6.25

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 32
AccB1I GGYRCC 3 cut(s) 32, 622, 1767
AccI GTMKAC 3 cut(s) 1632, 1750, 2479
AciI CCGC 5 cut(s) 469, 3101, 3542, 3609, 3656
AclI AACGTT 1 cut(s) 3619
AclWI GGATC 8 cut(s) 38, 51, 1173, 1793, 2559, 2892, 3462, 3620
AcuI CTGAAG 8 cut(s) 531, 1100, 1109, 1416, 1691, 2160, 2379, 2489
AfaI GTAC 7 cut(s) 34, 862, 1097, 2023, 2701, 2799, 2946
AfiI CCNNNNNNNGG 8 cut(s) 220, 937, 2188, 2665, 2977, 3132, 3186, 3538
AflII CTTAAG 2 cut(s) 1907, 3263
AflIII ACRYGT 3 cut(s) 857, 1360, 1504
AjiI CACGTC 1 cut(s) 3668
AjnI CCWGG 2 cut(s) 2228, 2266
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AloI GAACNNNNNNTCC 2 cut(s) 2668, 2700
Alw21I GWGCWC 3 cut(s) 918, 985, 2898
Alw26I GTCTC 8 cut(s) 236, 629, 848, 1320, 1697, 2179, 2289, 3689
AlwI GGATC 8 cut(s) 38, 51, 1173, 1793, 2559, 2892, 3462, 3620
AlwNI CAGNNNCTG 3 cut(s) 756, 2467, 3161
AoxI GGCC 4 cut(s) 104, 322, 2264, 2925
ApeKI GCWGC 8 cut(s) 866, 1927, 2003, 2321, 2532, 3061, 3080, 3176
ArsI GACNNNNNNTTYG 2 cut(s) 1582, 1614
Asp700I GAANNNNTTC 5 cut(s) 435, 1379, 2145, 2680, 3644
Asp718I GGTACC 1 cut(s) 32
AspLEI GCGC 3 cut(s) 366, 1036, 1291
AspS9I GGNCC 3 cut(s) 211, 322, 1007
AsuHPI GGTGA 5 cut(s) 1004, 2144, 2771, 2972, 3242
AvaII GGWCC 2 cut(s) 211, 1007
AxyI CCTNAGG 1 cut(s) 2261
BaeGI GKGCMC 1 cut(s) 627
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 3 cut(s) 32, 622, 1767
BanII GRGCYC 5 cut(s) 918, 2051, 2697, 3193, 3492
BauI CACGAG 1 cut(s) 367
BbsI GAAGAC 5 cut(s) 169, 1503, 1805, 2604, 2772
Bbv12I GWGCWC 3 cut(s) 918, 985, 2898
BbvCI CCTCAGC 1 cut(s) 1292
BbvI GCAGC 8 cut(s) 878, 1914, 1990, 2308, 2519, 3067, 3073, 3163
BccI CCATC 8 cut(s) 115, 820, 1046, 1202, 1336, 1790, 1802, 2182
BceAI ACGGC 2 cut(s) 312, 3295
BciT130I CCWGG 2 cut(s) 2230, 2268
BclI TGATCA 5 cut(s) 489, 1234, 2194, 2950, 3316
BcoDI GTCTC 8 cut(s) 236, 629, 848, 1320, 1697, 2179, 2289, 3689
BfaI CTAG 7 cut(s) 2034, 2342, 2606, 3251, 3425, 3554, 3606
BfmI CTRYAG 4 cut(s) 813, 864, 2004, 3483
BfoI RGCGCY 2 cut(s) 1037, 1292
BfrI CTTAAG 2 cut(s) 1907, 3263
BglII AGATCT 2 cut(s) 952, 1557
BisI GCNGC 9 cut(s) 867, 1928, 2004, 2322, 2533, 3062, 3081, 3177, 3657
BlsI GCNGC 9 cut(s) 868, 1929, 2005, 2323, 2534, 3063, 3082, 3178, 3658
BmcAI AGTACT 1 cut(s) 2701
Bme1390I CCNGG 2 cut(s) 2230, 2268
Bme18I GGWCC 2 cut(s) 211, 1007
BmgBI CACGTC 1 cut(s) 3668
BmgT120I GGNCC 3 cut(s) 211, 322, 1007
BmiI GGNNCC 5 cut(s) 34, 45, 323, 624, 1769
BmrFI CCNGG 2 cut(s) 2230, 2268
BmrI ACTGGG 1 cut(s) 648
BmuI ACTGGG 1 cut(s) 648
BpiI GAAGAC 5 cut(s) 169, 1503, 1805, 2604, 2772
BpmI CTGGAG 2 cut(s) 146, 2521
Bpu10I CCTNAGC 1 cut(s) 1292
BpuEI CTTGAG 6 cut(s) 1040, 1721, 1997, 2096, 2255, 2808
BsaAI YACGTR 1 cut(s) 860
BsaBI GATNNNNATC 1 cut(s) 2883
BsaI GGTCTC 1 cut(s) 2289
BsaJI CCNNGG 4 cut(s) 1854, 2616, 3198, 3447
BsaWI WCCGGW 1 cut(s) 2756
BsaXI ACNNNNNCTCC 4 cut(s) 2668, 2698, 3487, 3517
Bsc4I CCNNNNNNNGG 8 cut(s) 220, 937, 2188, 2665, 2977, 3132, 3186, 3538
Bse1I ACTGG 4 cut(s) 643, 767, 1870, 2538
Bse21I CCTNAGG 1 cut(s) 2261
Bse3DI GCAATG 3 cut(s) 1937, 2432, 3333
Bse8I GATNNNNATC 1 cut(s) 2883
BseBI CCWGG 2 cut(s) 2230, 2268
BseDI CCNNGG 4 cut(s) 1854, 2616, 3198, 3447
BseGI GGATG 9 cut(s) 1030, 1057, 1302, 1483, 1543, 1701, 1987, 2428, 2724
BseJI GATNNNNATC 1 cut(s) 2883
BseLI CCNNNNNNNGG 8 cut(s) 220, 937, 2188, 2665, 2977, 3132, 3186, 3538
BseMI GCAATG 3 cut(s) 1937, 2432, 3333
BseNI ACTGG 4 cut(s) 643, 767, 1870, 2538
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 8 cut(s) 878, 1914, 1990, 2308, 2519, 3067, 3073, 3163
BsgI GTGCAG 2 cut(s) 1297, 3541
BshFI GGCC 4 cut(s) 106, 324, 2266, 2927
BshNI GGYRCC 3 cut(s) 32, 622, 1767
BsiHKAI GWGCWC 3 cut(s) 918, 985, 2898
BsiSI CCGG 1 cut(s) 2757
BslI CCNNNNNNNGG 8 cut(s) 220, 937, 2188, 2665, 2977, 3132, 3186, 3538
BsmAI GTCTC 8 cut(s) 236, 629, 848, 1320, 1697, 2179, 2289, 3689
BsmI GAATGC 2 cut(s) 155, 2651
BsnI GGCC 4 cut(s) 106, 324, 2266, 2927
Bso31I GGTCTC 1 cut(s) 2289
Bsp1286I GDGCHC 8 cut(s) 627, 918, 985, 2051, 2697, 2898, 3193, 3492
Bsp1407I TGTACA 1 cut(s) 2944
Bsp19I CCATGG 2 cut(s) 1854, 2616
BspACI CCGC 5 cut(s) 469, 3101, 3542, 3609, 3656
BspANI GGCC 4 cut(s) 106, 324, 2266, 2927
BspHI TCATGA 1 cut(s) 2953
BspLI GGNNCC 5 cut(s) 34, 45, 323, 624, 1769
BspMAI CTGCAG 3 cut(s) 817, 868, 2008
BspPI GGATC 8 cut(s) 38, 51, 1173, 1793, 2559, 2892, 3462, 3620
BspT107I GGYRCC 3 cut(s) 32, 622, 1767
BspTI CTTAAG 2 cut(s) 1907, 3263
BspTNI GGTCTC 1 cut(s) 2289
BsrDI GCAATG 3 cut(s) 1937, 2432, 3333
BsrGI TGTACA 1 cut(s) 2944
BsrI ACTGG 4 cut(s) 643, 767, 1870, 2538
BssECI CCNNGG 4 cut(s) 1854, 2616, 3198, 3447
BssNAI GTATAC 1 cut(s) 2480
BssSI CACGAG 1 cut(s) 367
BssT1I CCWWGG 3 cut(s) 1854, 2616, 3198
Bst1107I GTATAC 1 cut(s) 2480
Bst2BI CACGAG 1 cut(s) 367
Bst2UI CCWGG 2 cut(s) 2230, 2268
Bst4CI ACNGT 8 cut(s) 69, 435, 569, 1063, 1111, 1708, 1721, 2391
Bst6I CTCTTC 3 cut(s) 33, 2465, 3426
BstAFI CTTAAG 2 cut(s) 1907, 3263
BstAPI GCANNNNNTGC 4 cut(s) 60, 2586, 3161, 3332
BstAUI TGTACA 1 cut(s) 2944
BstBAI YACGTR 1 cut(s) 860
BstDSI CCRYGG 3 cut(s) 1854, 2616, 3447
BstF5I GGATG 9 cut(s) 1030, 1057, 1302, 1483, 1543, 1701, 1987, 2428, 2724
BstH2I RGCGCY 2 cut(s) 1037, 1292
BstHHI GCGC 3 cut(s) 366, 1036, 1291
BstMAI GTCTC 8 cut(s) 236, 629, 848, 1320, 1697, 2179, 2289, 3689
BstNI CCWGG 2 cut(s) 2230, 2268
BstNSI RCATGY 9 cut(s) 18, 54, 1364, 1508, 2084, 2636, 2832, 3417, 3515
BstSCI CCNGG 2 cut(s) 2228, 2266
BstSFI CTRYAG 4 cut(s) 813, 864, 2004, 3483
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 8 cut(s) 878, 1914, 1990, 2308, 2519, 3067, 3073, 3163
BstV2I GAAGAC 5 cut(s) 169, 1503, 1805, 2604, 2772
BstX2I RGATCY 4 cut(s) 43, 952, 1557, 2884
BstYI RGATCY 4 cut(s) 43, 952, 1557, 2884
BstZ17I GTATAC 1 cut(s) 2480
Bsu36I CCTNAGG 1 cut(s) 2261
BsuRI GGCC 4 cut(s) 106, 324, 2266, 2927
BtgI CCRYGG 3 cut(s) 1854, 2616, 3447
BtrI CACGTC 1 cut(s) 3668
BtsCI GGATG 9 cut(s) 1030, 1057, 1302, 1483, 1543, 1701, 1987, 2428, 2724
BtsI GCAGTG 1 cut(s) 3155
BtsIMutI CAGTG 8 cut(s) 282, 1280, 1863, 2176, 2396, 2545, 2896, 3155
CaiI CAGNNNCTG 3 cut(s) 756, 2467, 3161
CciI TCATGA 1 cut(s) 2953
CfoI GCGC 3 cut(s) 366, 1036, 1291
Cfr13I GGNCC 3 cut(s) 211, 322, 1007
CseI GACGC 2 cut(s) 1748, 2711
Csp6I GTAC 7 cut(s) 33, 861, 1096, 2022, 2700, 2798, 2945
CspCI CAANNNNNGTGG 2 cut(s) 2487, 2522
CviQI GTAC 7 cut(s) 33, 861, 1096, 2022, 2700, 2798, 2945
DraI TTTAAA 1 cut(s) 2922
Eam1104I CTCTTC 3 cut(s) 33, 2465, 3426
EarI CTCTTC 3 cut(s) 33, 2465, 3426
EciI GGCGGA 1 cut(s) 3557
Ecl136II GAGCTC 1 cut(s) 916
Eco130I CCWWGG 3 cut(s) 1854, 2616, 3198
Eco24I GRGCYC 5 cut(s) 918, 2051, 2697, 3193, 3492
Eco31I GGTCTC 1 cut(s) 2289
Eco32I GATATC 4 cut(s) 726, 1464, 2663, 2965
Eco47I GGWCC 2 cut(s) 211, 1007
Eco53kI GAGCTC 1 cut(s) 916
Eco57I CTGAAG 8 cut(s) 531, 1100, 1109, 1416, 1691, 2160, 2379, 2489
Eco81I CCTNAGG 1 cut(s) 2261
EcoICRI GAGCTC 1 cut(s) 916
EcoO109I RGGNCCY 1 cut(s) 1007
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 2228, 2266
EcoRV GATATC 4 cut(s) 726, 1464, 2663, 2965
EcoT14I CCWWGG 3 cut(s) 1854, 2616, 3198
EcoT22I ATGCAT 1 cut(s) 56
EcoT38I GRGCYC 5 cut(s) 918, 2051, 2697, 3193, 3492
ErhI CCWWGG 3 cut(s) 1854, 2616, 3198
FalI AAGNNNNNCTT 4 cut(s) 2110, 2142, 2133, 2165
FauI CCCGC 2 cut(s) 462, 3602
FauNDI CATATG 1 cut(s) 3404
FbaI TGATCA 5 cut(s) 489, 1234, 2194, 2950, 3316
FblI GTMKAC 3 cut(s) 1632, 1750, 2479
Fnu4HI GCNGC 9 cut(s) 867, 1928, 2004, 2322, 2533, 3062, 3081, 3177, 3657
FokI GGATG 9 cut(s) 1037, 1064, 1309, 1490, 1550, 1708, 1994, 2435, 2731
FriOI GRGCYC 5 cut(s) 918, 2051, 2697, 3193, 3492
Fsp4HI GCNGC 9 cut(s) 867, 1928, 2004, 2322, 2533, 3062, 3081, 3177, 3657
FspBI CTAG 7 cut(s) 2034, 2342, 2606, 3251, 3425, 3554, 3606
GlaI GCGC 3 cut(s) 365, 1035, 1290
GluI GCNGC 9 cut(s) 867, 1928, 2004, 2322, 2533, 3062, 3081, 3177, 3657
GsuI CTGGAG 2 cut(s) 146, 2521
HaeII RGCGCY 2 cut(s) 1037, 1292
HaeIII GGCC 4 cut(s) 106, 324, 2266, 2927
HapII CCGG 1 cut(s) 2757
HgaI GACGC 2 cut(s) 1748, 2711
HhaI GCGC 3 cut(s) 366, 1036, 1291
Hin6I GCGC 3 cut(s) 364, 1034, 1289
HinP1I GCGC 3 cut(s) 364, 1034, 1289
HincII GTYRAC 2 cut(s) 1066, 1633
HindII GTYRAC 2 cut(s) 1066, 1633
HindIII AAGCTT 2 cut(s) 1082, 3674
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 1 cut(s) 2757
HphI GGTGA 5 cut(s) 1004, 2144, 2771, 2972, 3242
Hpy99I CGWCG 1 cut(s) 3669
HpyCH4III ACNGT 8 cut(s) 69, 435, 569, 1063, 1111, 1708, 1721, 2391
HpyCH4IV ACGT 4 cut(s) 859, 3382, 3619, 3667
HpySE526I ACGT 4 cut(s) 859, 3382, 3619, 3667
HspAI GCGC 3 cut(s) 364, 1034, 1289
KpnI GGTACC 1 cut(s) 36
Ksp22I TGATCA 5 cut(s) 489, 1234, 2194, 2950, 3316
KspAI GTTAAC 1 cut(s) 1066
LmnI GCTCC 5 cut(s) 127, 921, 1211, 2540, 3077
Lsp1109I GCAGC 8 cut(s) 878, 1914, 1990, 2308, 2519, 3067, 3073, 3163
MaeI CTAG 7 cut(s) 2034, 2342, 2606, 3251, 3425, 3554, 3606
MaeII ACGT 4 cut(s) 859, 3382, 3619, 3667
MaeIII GTNAC 4 cut(s) 629, 1356, 1966, 3338
MfeI CAATTG 2 cut(s) 870, 1046
MflI RGATCY 4 cut(s) 43, 952, 1557, 2884
MhlI GDGCHC 8 cut(s) 627, 918, 985, 2051, 2697, 2898, 3193, 3492
MlyI GAGTC 5 cut(s) 159, 1667, 2458, 3018, 3554
MmeI TCCRAC 2 cut(s) 2360, 3073
Mph1103I ATGCAT 1 cut(s) 56
MroXI GAANNNNTTC 5 cut(s) 435, 1379, 2145, 2680, 3644
MslI CAYNNNNRTG 5 cut(s) 118, 1505, 2487, 2952, 2958
MspA1I CMGCKG 1 cut(s) 968
MspCI CTTAAG 2 cut(s) 1907, 3263
MspI CCGG 1 cut(s) 2757
MspR9I CCNGG 2 cut(s) 2230, 2268
MunI CAATTG 2 cut(s) 870, 1046
Mva1269I GAATGC 2 cut(s) 155, 2651
MvaI CCWGG 2 cut(s) 2230, 2268
NcoI CCATGG 2 cut(s) 1854, 2616
NdeI CATATG 1 cut(s) 3404
NlaIV GGNNCC 5 cut(s) 34, 45, 323, 624, 1769
NmuCI GTSAC 1 cut(s) 3338
NsiI ATGCAT 1 cut(s) 56
NspI RCATGY 9 cut(s) 18, 54, 1364, 1508, 2084, 2636, 2832, 3417, 3515
PaeI GCATGC 3 cut(s) 2084, 2832, 3515
PagI TCATGA 1 cut(s) 2953
PciI ACATGT 2 cut(s) 1360, 1504
PctI GAATGC 2 cut(s) 155, 2651
PdmI GAANNNNTTC 5 cut(s) 435, 1379, 2145, 2680, 3644
PfeI GAWTC 7 cut(s) 385, 460, 698, 971, 1845, 3182, 3565
PflFI GACNNNGTC 1 cut(s) 168
PkrI GCNGC 9 cut(s) 868, 1929, 2005, 2323, 2534, 3063, 3082, 3178, 3658
PleI GAGTC 5 cut(s) 159, 1666, 2458, 3017, 3553
PpsI GAGTC 5 cut(s) 159, 1666, 2458, 3017, 3553
Ppu21I YACGTR 1 cut(s) 860
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 2 cut(s) 1360, 1504
Psp124BI GAGCTC 1 cut(s) 918
Psp1406I AACGTT 1 cut(s) 3619
Psp5II RGGWCCY 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 2228, 2266
PspGI CCWGG 2 cut(s) 2228, 2266
PspN4I GGNNCC 5 cut(s) 34, 45, 323, 624, 1769
PspPI GGNCC 3 cut(s) 211, 322, 1007
PspPPI RGGWCCY 1 cut(s) 1007
PstI CTGCAG 3 cut(s) 817, 868, 2008
PstNI CAGNNNCTG 3 cut(s) 756, 2467, 3161
PsuI RGATCY 4 cut(s) 43, 952, 1557, 2884
PsyI GACNNNGTC 1 cut(s) 168
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 7 cut(s) 34, 862, 1097, 2023, 2701, 2799, 2946
RsaNI GTAC 7 cut(s) 33, 861, 1096, 2022, 2700, 2798, 2945
RseI CAYNNNNRTG 5 cut(s) 118, 1505, 2487, 2952, 2958
SacI GAGCTC 1 cut(s) 918
SalI GTCGAC 1 cut(s) 1631
SatI GCNGC 9 cut(s) 867, 1928, 2004, 2322, 2533, 3062, 3081, 3177, 3657
Sau96I GGNCC 3 cut(s) 211, 322, 1007
ScaI AGTACT 1 cut(s) 2701
SchI GAGTC 5 cut(s) 159, 1667, 2458, 3018, 3554
ScrFI CCNGG 2 cut(s) 2230, 2268
SduI GDGCHC 8 cut(s) 627, 918, 985, 2051, 2697, 2898, 3193, 3492
SfcI CTRYAG 4 cut(s) 813, 864, 2004, 3483
SinI GGWCC 2 cut(s) 211, 1007
SmiMI CAYNNNNRTG 5 cut(s) 118, 1505, 2487, 2952, 2958
SmlI CTYRAG 8 cut(s) 1019, 1700, 1907, 1976, 2075, 2234, 2823, 3263
SmoI CTYRAG 8 cut(s) 1019, 1700, 1907, 1976, 2075, 2234, 2823, 3263
SphI GCATGC 3 cut(s) 2084, 2832, 3515
SsiI CCGC 5 cut(s) 469, 3101, 3542, 3609, 3656
SspMI CTAG 7 cut(s) 2034, 2342, 2606, 3251, 3425, 3554, 3606
SstI GAGCTC 1 cut(s) 918
StyD4I CCNGG 2 cut(s) 2228, 2266
StyI CCWWGG 3 cut(s) 1854, 2616, 3198
TaaI ACNGT 8 cut(s) 69, 435, 569, 1063, 1111, 1708, 1721, 2391
TaiI ACGT 4 cut(s) 862, 3385, 3622, 3670
TaqI TCGA 4 cut(s) 573, 985, 1632, 3615
TatI WGTACW 3 cut(s) 2699, 2797, 2944
TauI GCSGC 1 cut(s) 3659
TfiI GAWTC 7 cut(s) 385, 460, 698, 971, 1845, 3182, 3565
TscAI CASTG 8 cut(s) 289, 1280, 1870, 2176, 2396, 2545, 2896, 3155
TseFI GTSAC 1 cut(s) 3338
TseI GCWGC 8 cut(s) 866, 1927, 2003, 2321, 2532, 3061, 3080, 3176
Tsp45I GTSAC 1 cut(s) 3338
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 8 cut(s) 289, 1280, 1870, 2176, 2396, 2545, 2896, 3155
Tth111I GACNNNGTC 1 cut(s) 168
Vha464I CTTAAG 2 cut(s) 1907, 3263
VpaK11BI GGWCC 2 cut(s) 211, 1007
XbaI TCTAGA 1 cut(s) 3250
XceI RCATGY 9 cut(s) 18, 54, 1364, 1508, 2084, 2636, 2832, 3417, 3515
XcmI CCANNNNNNNNNTGG 1 cut(s) 2545
XmiI GTMKAC 3 cut(s) 1632, 1750, 2479
XmnI GAANNNNTTC 5 cut(s) 435, 1379, 2145, 2680, 3644
XspI CTAG 7 cut(s) 2034, 2342, 2606, 3251, 3425, 3554, 3606
ZrmI AGTACT 1 cut(s) 2701
Zsp2I ATGCAT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.