FvH4_5g06180

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006816
Physical Location & Seq
Forward (+)
3629686 .. 3631236
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06180.t2

Sequence Viewer

Length: 606 bp
ATGGGAGCTGGCAACCAAACCAAACCCGCCGCCGAAACCCTAAAATTCCTCTGCAGCTACGGCGGAAAGATCCTCCTCCGCCACACCGACGGCGAGCTCCGTTACGTCGGCGGCCACACTCGCGTCCTCTCCGTCGATCCCTCCATTTCCTTCTCTGAATTAATGGTGAAGCTCACTGAGTTCTGCGGCCAGTCCGTCACTCTCAGGTGTCAATTGCCGGACGGCGACTTGGAGACGTTGATTACGATCAAGTCGGATGAGGATTTGGCGAATCTAATCGAAGAGTACGAACGTGTTTCTTCATCTTCGTCTCGTTCATATAAGGTCAGAGCGATTCTCTCGCCGCCGAAATCGCTGAAGCAAGTCTCGCCAGCTCAGTCCACAACGTCGACCGGCGGCGACTGTTCGCCGTCGAAATCGCTCTACTCCGACTTCGATTCGCCGCCGAAGAGGTTCTATTCGCCGACGGCGGTCGGTTATCAGAGAGGGACGGGGAAAGTGTGCTACTATCCTTGCCATGTCCAGCGAACTCCCTGCGAGATTCCGTATAAGCACCACCGGTATCACCAGCACCTCCATCAATTTCAAGTCCCTCACTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

202

Amino Acids

22.4

Weight (kDa)

8.58

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 3 - 50 1.8e-09 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 389
AccII CGCG 1 cut(s) 123
AclWI GGATC 2 cut(s) 64, 131
AcoI YGGCCR 2 cut(s) 112, 187
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 292
AgeI ACCGGT 1 cut(s) 558
AgsI TTSAA 1 cut(s) 587
AluBI AGCT 5 cut(s) 8, 57, 97, 172, 374
AluI AGCT 5 cut(s) 8, 57, 97, 172, 374
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 3 cut(s) 227, 315, 370
AlwI GGATC 2 cut(s) 64, 131
AoxI GGCC 2 cut(s) 112, 187
ApeKI GCWGC 1 cut(s) 54
ApoI RAATTY 1 cut(s) 44
AseI ATTAAT 1 cut(s) 161
AsiGI ACCGGT 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 178, 557
BanII GRGCYC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 66
BccI CCATC 1 cut(s) 585
BceAI ACGGC 5 cut(s) 76, 106, 238, 394, 483
BcgI CGANNNNNNTGC 2 cut(s) 516, 550
BcoDI GTCTC 3 cut(s) 227, 315, 370
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 7 cut(s) 30, 55, 112, 187, 344, 397, 443
BlsI GCNGC 7 cut(s) 31, 56, 113, 188, 345, 398, 444
BoxI GACNNNNGTC 1 cut(s) 470
BplI GAGNNNNNCTC 2 cut(s) 321, 353
BsaBI GATNNNNATC 1 cut(s) 245
BsaWI WCCGGW 1 cut(s) 558
Bse118I RCCGGY 2 cut(s) 392, 558
Bse1I ACTGG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 245
BseGI GGATG 1 cut(s) 262
BseJI GATNNNNATC 1 cut(s) 245
BseMII CTCAG 3 cut(s) 168, 217, 389
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 2 cut(s) 393, 474
BshFI GGCC 2 cut(s) 114, 189
BshTI ACCGGT 1 cut(s) 558
BsiEI CGRYCG 2 cut(s) 393, 474
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 3 cut(s) 218, 393, 559
BslFI GGGAC 2 cut(s) 502, 575
BsmAI GTCTC 3 cut(s) 227, 315, 370
BsmBI CGTCTC 2 cut(s) 227, 315
BsmFI GGGAC 2 cut(s) 502, 575
BsnI GGCC 2 cut(s) 114, 189
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 3 cut(s) 69, 136, 246
BspANI GGCC 2 cut(s) 114, 189
BspCNI CTCAG 3 cut(s) 169, 216, 388
BspFNI CGCG 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 64, 131
BsrFI RCCGGY 2 cut(s) 392, 558
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 2 cut(s) 392, 558
BssMI GATC 3 cut(s) 69, 136, 246
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 2 cut(s) 276, 443
BstC8I GCNNGC 3 cut(s) 10, 95, 372
BstDEI CTNAG 3 cut(s) 177, 203, 375
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 3 cut(s) 72, 139, 249
BstMAI GTCTC 3 cut(s) 227, 315, 370
BstMBI GATC 3 cut(s) 69, 136, 246
BstMCI CGRYCG 2 cut(s) 393, 474
BstMWI GCNNNNNNNGC 4 cut(s) 60, 120, 352, 367
BstPAI GACNNNNGTC 1 cut(s) 470
BstSFI CTRYAG 1 cut(s) 52
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 2 cut(s) 114, 189
BtsCI GGATG 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 2 cut(s) 174, 595
Cac8I GCNNGC 3 cut(s) 10, 95, 372
Cfr10I RCCGGY 2 cut(s) 392, 558
CseI GACGC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 286
CspAI ACCGGT 1 cut(s) 558
CviAII CATG 1 cut(s) 518
CviJI RGCY 7 cut(s) 8, 57, 97, 114, 172, 189, 374
CviKI_1 RGCY 7 cut(s) 8, 57, 97, 114, 172, 189, 374
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 3 cut(s) 177, 203, 375
DpnI GATC 3 cut(s) 71, 138, 248
DpnII GATC 3 cut(s) 69, 136, 246
EaeI YGGCCR 2 cut(s) 112, 187
Eam1104I CTCTTC 2 cut(s) 276, 443
EarI CTCTTC 2 cut(s) 276, 443
EciI GGCGGA 2 cut(s) 68, 78
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 2 cut(s) 227, 315
FaeI CATG 1 cut(s) 521
FaiI YATR 4 cut(s) 319, 321, 519, 549
FaqI GGGAC 2 cut(s) 502, 575
FatI CATG 1 cut(s) 517
FauI CCCGC 1 cut(s) 34
FblI GTMKAC 1 cut(s) 389
Fnu4HI GCNGC 7 cut(s) 30, 55, 112, 187, 344, 397, 443
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 7 cut(s) 30, 55, 112, 187, 344, 397, 443
GluI GCNGC 7 cut(s) 30, 55, 112, 187, 344, 397, 443
HaeIII GGCC 2 cut(s) 114, 189
HapII CCGG 3 cut(s) 218, 393, 559
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 521
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 4 cut(s) 271, 334, 437, 541
HpaII CCGG 3 cut(s) 218, 393, 559
HphI GGTGA 2 cut(s) 178, 557
Hpy166II GTNNAC 2 cut(s) 381, 390
Hpy188I TCNGA 5 cut(s) 157, 256, 329, 430, 483
Hpy8I GTNNAC 2 cut(s) 381, 390
Hpy99I CGWCG 6 cut(s) 92, 110, 137, 391, 415, 469
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 4 cut(s) 105, 236, 292, 386
HpyCH4V TGCA 2 cut(s) 54, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 120, 352, 367
HpyF3I CTNAG 3 cut(s) 177, 203, 375
HpySE526I ACGT 4 cut(s) 105, 236, 292, 386
Hsp92II CATG 1 cut(s) 521
Kzo9I GATC 3 cut(s) 69, 136, 246
LmnI GCTCC 2 cut(s) 5, 102
LpnPI CCDG 9 cut(s) 190, 203, 231, 384, 406, 536, 547, 572, 581
Lsp1109I GCAGC 1 cut(s) 66
MaeII ACGT 4 cut(s) 105, 236, 292, 386
MaeIII GTNAC 2 cut(s) 101, 196
MalI GATC 3 cut(s) 71, 138, 248
MboI GATC 3 cut(s) 69, 136, 246
MboII GAAGA 4 cut(s) 291, 293, 297, 460
MfeI CAATTG 2 cut(s) 212, 601
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 5 cut(s) 44, 158, 212, 581, 601
MmeI TCCRAC 2 cut(s) 234, 453
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 1 cut(s) 161
MspI CCGG 3 cut(s) 218, 393, 559
MunI CAATTG 2 cut(s) 212, 601
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 4 cut(s) 60, 120, 352, 367
NdeII GATC 3 cut(s) 69, 136, 246
NlaIII CATG 1 cut(s) 521
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 2 cut(s) 242, 285
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 4 cut(s) 271, 334, 437, 541
PinAI ACCGGT 1 cut(s) 558
PkrI GCNGC 7 cut(s) 31, 56, 113, 188, 345, 398, 444
PshAI GACNNNNGTC 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 99
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 69
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 388
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 7 cut(s) 30, 55, 112, 187, 344, 397, 443
Sau3AI GATC 3 cut(s) 69, 136, 246
SduI GDGCHC 1 cut(s) 99
SfcI CTRYAG 1 cut(s) 52
Sse9I AATT 5 cut(s) 44, 158, 212, 581, 601
SstI GAGCTC 1 cut(s) 99
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 4 cut(s) 108, 239, 295, 389
TaqI TCGA 5 cut(s) 135, 279, 389, 413, 435
TasI AATT 5 cut(s) 44, 158, 212, 581, 601
TauI GCSGC 6 cut(s) 32, 114, 189, 346, 399, 445
TfiI GAWTC 4 cut(s) 271, 334, 437, 541
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 2 cut(s) 181, 602
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 291, 306
TspGWI ACGGA 4 cut(s) 89, 121, 184, 534
TspRI CASTG 2 cut(s) 181, 602
VspI ATTAAT 1 cut(s) 161
XapI RAATTY 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 389
XmnI GAANNNNTTC 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.