Rh7DG130800

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006816
Physical Location & Seq
Reverse (-)
10991077 .. 10991778
702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG130800.1

Sequence Viewer

Length: 597 bp
ATGGTAGCTGGCAACCAAACCAAAACCGCCACCGAAACCCTAAAATTCCTCTGCAGCTACGGCGGCAAGATCCTCCTCCGCCACACCGACGGCCAGCTCCGCTACGTCGGCGGCCACACCCGCGTCCTCTCCGTCGATCCCTCCATTTCCTTCTCAGAATTAATGGTGAAGCTCGCTGAGTTCTGCGGCCACTCCGTCACTCTCAGGTGTCAATTGCTGGGCGGCGACTTGGAGACGTTGATTTCGATCAAGTCCGACGAGGATTTGGCGAATCTAATAGAAGAGTACGATCGTGTTTCTCCTTCTCGTTCCTATAAGATCAGAGCGATTCTGTCTCCCCCGAAACCGCTGAAACAAGTCTCGCCTGCTCAGTCCACAACATCGACCAGCGGCGACTGTTCGCCGTCGAAATCGCTCTATTCCGACTTCGATTCGCCGCCGAAGCGTTACTATTCCCCGACGGCGGTCGGGTTTCAGAGAGGTCCGGGAAAAGTGTGCTACTATCCTTGCCATGTCCAGCGAACTCCCTGCGAGATTCCGTATCAGCATCACCGTTATCACCAGCACCCCCATCAATTTCACGCTCCCTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

198

Amino Acids

22.1

Weight (kDa)

8.75

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 30 - 107 2.2e-18 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 123
AclWI GGATC 2 cut(s) 64, 131
AcoI YGGCCR 3 cut(s) 91, 112, 187
AcsI RAATTY 1 cut(s) 44
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 463
AluBI AGCT 4 cut(s) 8, 57, 97, 172
AluI AGCT 4 cut(s) 8, 57, 97, 172
Alw26I GTCTC 3 cut(s) 227, 339, 364
AlwI GGATC 2 cut(s) 64, 131
AoxI GGCC 3 cut(s) 91, 112, 187
ApeKI GCWGC 1 cut(s) 54
ApoI RAATTY 1 cut(s) 44
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AseI ATTAAT 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 482
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 3 cut(s) 178, 542, 551
AvaII GGWCC 1 cut(s) 482
BbvI GCAGC 1 cut(s) 66
BccI CCATC 1 cut(s) 579
BceAI ACGGC 4 cut(s) 76, 106, 388, 477
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 3 cut(s) 227, 339, 364
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 391, 437
BlsI GCNGC 7 cut(s) 56, 65, 113, 188, 224, 392, 438
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmrFI CCNGG 1 cut(s) 486
BmsI GCATC 1 cut(s) 556
BoxI GACNNNNGTC 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 463
Bse8I GATNNNNATC 1 cut(s) 245
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 463
BseMII CTCAG 4 cut(s) 168, 168, 217, 383
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 217
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 2 cut(s) 292, 468
BshFI GGCC 3 cut(s) 93, 114, 189
BsiEI CGRYCG 2 cut(s) 292, 468
BsiSI CCGG 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 463
BsmAI GTCTC 3 cut(s) 227, 339, 364
BsmBI CGTCTC 1 cut(s) 227
BsnI GGCC 3 cut(s) 93, 114, 189
Bsp143I GATC 5 cut(s) 69, 136, 246, 289, 318
BspANI GGCC 3 cut(s) 93, 114, 189
BspCNI CTCAG 4 cut(s) 167, 169, 216, 382
BspFNI CGCG 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 64, 131
BssMI GATC 5 cut(s) 69, 136, 246, 289, 318
Bst4CI ACNGT 2 cut(s) 398, 554
Bst6I CTCTTC 1 cut(s) 276
BstC8I GCNNGC 4 cut(s) 10, 95, 174, 366
BstDEI CTNAG 4 cut(s) 154, 177, 203, 369
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 5 cut(s) 72, 139, 249, 292, 321
BstMAI GTCTC 3 cut(s) 227, 339, 364
BstMBI GATC 5 cut(s) 69, 136, 246, 289, 318
BstMCI CGRYCG 2 cut(s) 292, 468
BstMWI GCNNNNNNNGC 6 cut(s) 60, 63, 99, 108, 120, 442
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 52
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 3 cut(s) 93, 114, 189
Cac8I GCNNGC 4 cut(s) 10, 95, 174, 366
Cfr13I GGNCC 1 cut(s) 482
CseI GACGC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 286
CviAII CATG 1 cut(s) 512
CviJI RGCY 7 cut(s) 8, 57, 93, 97, 114, 172, 189
CviKI_1 RGCY 7 cut(s) 8, 57, 93, 97, 114, 172, 189
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 4 cut(s) 154, 177, 203, 369
DpnI GATC 5 cut(s) 71, 138, 248, 291, 320
DpnII GATC 5 cut(s) 69, 136, 246, 289, 318
EaeI YGGCCR 3 cut(s) 91, 112, 187
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 482
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 1 cut(s) 515
FaiI YATR 2 cut(s) 315, 513
FatI CATG 1 cut(s) 511
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 391, 437
Fsp4HI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 391, 437
GluI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 391, 437
GsaI CCCAGC 1 cut(s) 221
HaeIII GGCC 3 cut(s) 93, 114, 189
HapII CCGG 1 cut(s) 485
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 515
HinfI GANTC 4 cut(s) 271, 328, 431, 535
HpaII CCGG 1 cut(s) 485
HphI GGTGA 3 cut(s) 178, 542, 551
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 5 cut(s) 157, 256, 323, 424, 477
Hpy8I GTNNAC 1 cut(s) 375
Hpy99I CGWCG 6 cut(s) 92, 110, 137, 260, 409, 463
HpyAV CCTTC 2 cut(s) 160, 312
HpyCH4III ACNGT 2 cut(s) 398, 554
HpyCH4IV ACGT 2 cut(s) 105, 236
HpyCH4V TGCA 2 cut(s) 54, 591
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 63, 99, 108, 120, 442
HpyF3I CTNAG 4 cut(s) 154, 177, 203, 369
HpySE526I ACGT 2 cut(s) 105, 236
Hsp92II CATG 1 cut(s) 515
Kzo9I GATC 5 cut(s) 69, 136, 246, 289, 318
LmnI GCTCC 2 cut(s) 102, 589
LpnPI CCDG 9 cut(s) 107, 190, 203, 378, 400, 498, 530, 541, 575
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 556
MaeII ACGT 2 cut(s) 105, 236
MaeIII GTNAC 2 cut(s) 196, 446
MalI GATC 5 cut(s) 71, 138, 248, 291, 320
MboI GATC 5 cut(s) 69, 136, 246, 289, 318
MboII GAAGA 1 cut(s) 293
MfeI CAATTG 2 cut(s) 212, 592
MflI RGATCY 1 cut(s) 69
MluCI AATT 5 cut(s) 44, 158, 212, 575, 592
MmeI TCCRAC 2 cut(s) 279, 447
MnlI CCTC 7 cut(s) 59, 83, 86, 137, 151, 253, 473
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 349, 390
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 486
MunI CAATTG 2 cut(s) 212, 592
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 6 cut(s) 60, 63, 99, 108, 120, 442
NciI CCSGG 1 cut(s) 486
NdeII GATC 5 cut(s) 69, 136, 246, 289, 318
NlaIII CATG 1 cut(s) 515
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 4 cut(s) 271, 328, 431, 535
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 7 cut(s) 56, 65, 113, 188, 224, 392, 438
Ple19I CGATCG 1 cut(s) 292
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 482
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 69
PvuI CGATCG 1 cut(s) 292
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 391, 437
Sau3AI GATC 5 cut(s) 69, 136, 246, 289, 318
Sau96I GGNCC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 486
SetI ASST 8 cut(s) 10, 59, 99, 108, 174, 209, 239, 484
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 1 cut(s) 52
SinI GGWCC 1 cut(s) 482
Sse9I AATT 5 cut(s) 44, 158, 212, 575, 592
StyD4I CCNGG 1 cut(s) 484
TaaI ACNGT 2 cut(s) 398, 554
TaiI ACGT 2 cut(s) 108, 239
TaqI TCGA 5 cut(s) 135, 245, 383, 407, 429
TasI AATT 5 cut(s) 44, 158, 212, 575, 592
TauI GCSGC 6 cut(s) 66, 114, 189, 225, 393, 439
TfiI GAWTC 4 cut(s) 271, 328, 431, 535
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 3 cut(s) 121, 184, 528
VpaK11BI GGWCC 1 cut(s) 482
VspI ATTAAT 1 cut(s) 161
XapI RAATTY 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.