MD14G1172100.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006816
Physical Location & Seq
Reverse (-)
26572433 .. 26574549
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1172100.v1.1.491

Sequence Viewer

Length: 321 bp
ATGTCCACTGCTACGAGCGGCGGCGACTCGTCCCCTTCGAAATCGCCCTTCTCATCCACCGATTCGCCTCCGAAACGTTTCGTTTCGCCACAAAGACGGTACGTTTCGCCTCAAGTCTCGCCGTCGGCTGGACACCGCGCCGGGTATCAGAGAGGGTCCGGACGAGTCTGCTACTATCCTTGCCATGTCCACACTCAAAGGAGCAACCCCTGCGACATGTCGTATGTTCATCATTATCAGCAGTATCGTCACTACCACCACCACCAACACGTTCCTCACTGCGACATGTCGTATGCTCGGAGTTTCCCACAGAGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

107

Amino Acids

12.12

Weight (kDa)

9.69

Isoelectric Point (pI)

104.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 18
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 3 cut(s) 18, 21, 136
AclI AACGTT 1 cut(s) 76
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 128
AflIII ACRYGT 3 cut(s) 216, 268, 285
Alw26I GTCTC 1 cut(s) 121
Aor13HI TCCGGA 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 156
AsuC2I CCSGG 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 38
AvaII GGWCC 1 cut(s) 156
BceAI ACGGC 1 cut(s) 106
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 1 cut(s) 319
BisI GCNGC 2 cut(s) 19, 22
BlsI GCNGC 2 cut(s) 20, 23
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 156
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 142
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 128
BseAI TCCGGA 1 cut(s) 158
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 138
BsiSI CCGG 2 cut(s) 141, 159
BslFI GGGAC 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 16
Bsp119I TTCGAA 1 cut(s) 38
Bsp13I TCCGGA 1 cut(s) 158
BspACI CCGC 3 cut(s) 18, 21, 136
BspEI TCCGGA 1 cut(s) 158
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 157
BspT104I TTCGAA 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 18
Bst4CI ACNGT 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 38
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstMAI GTCTC 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstNSI RCATGY 2 cut(s) 220, 289
BstSCI CCNGG 1 cut(s) 140
BstUI CGCG 1 cut(s) 138
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 2 cut(s) 6, 277
BtsIMutI CAGTG 2 cut(s) 6, 277
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 156
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 185, 217, 286
CviJI RGCY 1 cut(s) 128
CviKI_1 RGCY 1 cut(s) 128
CviQI GTAC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 156
FaeI CATG 3 cut(s) 188, 220, 289
FaiI YATR 5 cut(s) 186, 218, 225, 287, 294
FaqI GGGAC 1 cut(s) 16
FatI CATG 3 cut(s) 184, 216, 285
Fnu4HI GCNGC 2 cut(s) 19, 22
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 2 cut(s) 19, 22
FspBI CTAG 1 cut(s) 319
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 19, 22
HapII CCGG 2 cut(s) 141, 159
HhaI GCGC 1 cut(s) 140
Hin1II CATG 3 cut(s) 188, 220, 289
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 3 cut(s) 26, 62, 165
HpaII CCGG 2 cut(s) 141, 159
Hpy166II GTNNAC 2 cut(s) 6, 190
Hpy188I TCNGA 3 cut(s) 72, 150, 300
Hpy188III TCNNGA 1 cut(s) 159
Hpy8I GTNNAC 2 cut(s) 6, 190
Hpy99I CGWCG 1 cut(s) 127
HpyAV CCTTC 2 cut(s) 45, 58
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4IV ACGT 3 cut(s) 76, 102, 270
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
HpySE526I ACGT 3 cut(s) 76, 102, 270
Hsp92II CATG 3 cut(s) 188, 220, 289
HspAI GCGC 1 cut(s) 138
Kpn2I TCCGGA 1 cut(s) 158
LmnI GCTCC 1 cut(s) 201
LpnPI CCDG 4 cut(s) 114, 154, 172, 223
MaeI CTAG 1 cut(s) 319
MaeII ACGT 3 cut(s) 76, 102, 270
MaeIII GTNAC 1 cut(s) 248
MbiI CCGCTC 1 cut(s) 18
MlyI GAGTC 2 cut(s) 20, 174
MnlI CCTC 4 cut(s) 78, 120, 146, 285
MroI TCCGGA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 77
MspI CCGG 2 cut(s) 141, 159
MspR9I CCNGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 210
NciI CCSGG 1 cut(s) 142
NlaIII CATG 3 cut(s) 188, 220, 289
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 248
NspI RCATGY 2 cut(s) 220, 289
NspV TTCGAA 1 cut(s) 38
PciI ACATGT 2 cut(s) 216, 285
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 20, 23
PleI GAGTC 2 cut(s) 20, 173
PpsI GAGTC 2 cut(s) 20, 173
PscI ACATGT 2 cut(s) 216, 285
Psp1406I AACGTT 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 156
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 2 cut(s) 19, 22
Sau96I GGNCC 1 cut(s) 156
SchI GAGTC 2 cut(s) 20, 174
ScrFI CCNGG 1 cut(s) 142
SetI ASST 4 cut(s) 79, 105, 273, 320
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SsiI CCGC 3 cut(s) 18, 21, 136
SspMI CTAG 1 cut(s) 319
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 99
TaiI ACGT 3 cut(s) 79, 105, 273
TaqI TCGA 1 cut(s) 38
TauI GCSGC 2 cut(s) 21, 24
TfiI GAWTC 1 cut(s) 62
TscAI CASTG 2 cut(s) 13, 284
TseFI GTSAC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 218
TspRI CASTG 2 cut(s) 13, 284
VpaK11BI GGWCC 1 cut(s) 156
XceI RCATGY 2 cut(s) 220, 289
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.