pycom14g14360

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006816
Physical Location & Seq
Reverse (-)
17079139 .. 17079949
811 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14360.1

Sequence Viewer

Length: 681 bp
ATGGACCTCAACAAAGCACCTGAACCGTTGAAGATCCTCTGCAGCTATGGTGGTAAAATCCTCCCCCGCCACTCCGACGGCACGCTCCGATACGTCGGCGGCCACAACCGCGTCCTCGCCGTCGATTCCTCCATTACATGTACAGAGCTAATGGAGAAGCTTAAGGAGTTCTGTGGCTACTCTGTGGAATTGAAGTGTCCATTGCCGAACGGAGATTTGGAGACCTTGATTGCCGTAAAGTCCGACGAGGATTTGACGAATATCATCGAAGAGTACGATCGAGCTTCTTCTCTGCCTCGTGCTCTGAAGATCAGAGCCATTCTCGCGCCACCGAAGTCCCCGAAGCAAGTCTCGCCTCCGATGTCCACTGCTACGAGCGGCGGCGACTCGTCCCCTTCGAAATCGCTCTTCTCATCGACCGATTCGCCTCCGAAACGTTTCGTTTCGCCACCAAGACGGTACGTTTCGCCTCAAGTCTCGCCGCCGGCTGGACACCGCGCCGGATATCAGAGAGGGTCCGGACGAGTCTGCTACTATCCTTGCCATGTCCACACTCAGAGGAGCAAGCCCTGCGACATGTCGTATGTTCATCATTATCAGCAGTATCGTCACTACCACCACCACCAACACGTTCCTCACTGCGACATGTCGTATGCTCGGAGCTTCCCACAGAGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

227

Amino Acids

25.3

Weight (kDa)

9.16

Isoelectric Point (pI)

75.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 29 - 96 4.7e-15 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 378
AccII CGCG 3 cut(s) 111, 326, 498
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 7 cut(s) 67, 99, 109, 378, 381, 482, 496
AclI AACGTT 1 cut(s) 436
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 100
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 3 cut(s) 142, 275, 461
AfiI CCNNNNNNNGG 1 cut(s) 488
AflII CTTAAG 1 cut(s) 161
AflIII ACRYGT 4 cut(s) 137, 576, 628, 645
AgsI TTSAA 2 cut(s) 31, 193
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 5 cut(s) 45, 148, 160, 284, 663
AluI AGCT 5 cut(s) 45, 148, 160, 284, 663
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 3 cut(s) 215, 355, 481
AlwI GGATC 1 cut(s) 28
Aor13HI TCCGGA 1 cut(s) 518
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 2 cut(s) 328, 500
AspS9I GGNCC 2 cut(s) 4, 516
AsuII TTCGAA 1 cut(s) 398
AvaII GGWCC 2 cut(s) 4, 516
BauI CACGAG 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 54
BceAI ACGGC 3 cut(s) 94, 104, 218
BcoDI GTCTC 3 cut(s) 215, 355, 481
BfaI CTAG 1 cut(s) 679
BfmI CTRYAG 1 cut(s) 40
BfrI CTTAAG 1 cut(s) 161
BisI GCNGC 5 cut(s) 43, 100, 379, 382, 482
BlsI GCNGC 5 cut(s) 44, 101, 380, 383, 483
Bme18I GGWCC 2 cut(s) 4, 516
BmgT120I GGNCC 2 cut(s) 4, 516
BmiI GGNNCC 1 cut(s) 517
BplI GAGNNNNNCTC 2 cut(s) 306, 338
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 215
BsaWI WCCGGW 1 cut(s) 518
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 1 cut(s) 484
Bse3DI GCAATG 1 cut(s) 200
BseAI TCCGGA 1 cut(s) 518
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 1 cut(s) 200
BseMII CTCAG 1 cut(s) 569
BseRI GAGGAG 1 cut(s) 574
BseXI GCAGC 1 cut(s) 54
Bsh1236I CGCG 3 cut(s) 111, 326, 498
Bsh1285I CGRYCG 2 cut(s) 280, 420
BshFI GGCC 1 cut(s) 102
BsiEI CGRYCG 2 cut(s) 280, 420
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 3 cut(s) 485, 501, 519
BslFI GGGAC 2 cut(s) 322, 376
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 3 cut(s) 215, 355, 481
BsmFI GGGAC 2 cut(s) 322, 376
BsnI GGCC 1 cut(s) 102
Bso31I GGTCTC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 304
Bsp13I TCCGGA 1 cut(s) 518
Bsp1407I TGTACA 1 cut(s) 140
Bsp143I GATC 3 cut(s) 33, 277, 309
BspACI CCGC 7 cut(s) 67, 99, 109, 378, 381, 482, 496
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 1 cut(s) 568
BspEI TCCGGA 1 cut(s) 518
BspFNI CGCG 3 cut(s) 111, 326, 498
BspLI GGNNCC 1 cut(s) 517
BspMAI CTGCAG 1 cut(s) 44
BspPI GGATC 1 cut(s) 28
BspQI GCTCTTC 1 cut(s) 413
BspT104I TTCGAA 1 cut(s) 398
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 484
BssMI GATC 3 cut(s) 33, 277, 309
BssSI CACGAG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 27, 459
Bst6I CTCTTC 2 cut(s) 264, 413
BstAFI CTTAAG 1 cut(s) 161
BstAPI GCANNNNNTGC 1 cut(s) 570
BstAUI TGTACA 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 83, 486, 566
BstDEI CTNAG 1 cut(s) 555
BstFNI CGCG 3 cut(s) 111, 326, 498
BstHHI GCGC 2 cut(s) 328, 500
BstKTI GATC 3 cut(s) 36, 280, 312
BstMAI GTCTC 3 cut(s) 215, 355, 481
BstMBI GATC 3 cut(s) 33, 277, 309
BstMCI CGRYCG 2 cut(s) 280, 420
BstMWI GCNNNNNNNGC 4 cut(s) 108, 323, 352, 570
BstNSI RCATGY 3 cut(s) 141, 580, 649
BstSFI CTRYAG 1 cut(s) 40
BstUI CGCG 3 cut(s) 111, 326, 498
BstV1I GCAGC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 1 cut(s) 102
BtsI GCAGTG 2 cut(s) 366, 637
BtsIMutI CAGTG 2 cut(s) 366, 637
Cac8I GCNNGC 3 cut(s) 83, 486, 566
CfoI GCGC 2 cut(s) 328, 500
Cfr10I RCCGGY 1 cut(s) 484
Cfr13I GGNCC 2 cut(s) 4, 516
CseI GACGC 1 cut(s) 100
Csp6I GTAC 3 cut(s) 141, 274, 460
CviAII CATG 4 cut(s) 138, 545, 577, 646
CviQI GTAC 3 cut(s) 141, 274, 460
DdeI CTNAG 1 cut(s) 555
DpnI GATC 3 cut(s) 35, 279, 311
DpnII GATC 3 cut(s) 33, 277, 309
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 264, 413
EarI CTCTTC 2 cut(s) 264, 413
Eco31I GGTCTC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 506
Eco47I GGWCC 2 cut(s) 4, 516
Eco57I CTGAAG 1 cut(s) 326
EcoRV GATATC 1 cut(s) 506
FaeI CATG 4 cut(s) 141, 548, 580, 649
FaiI YATR 7 cut(s) 48, 139, 546, 578, 585, 647, 654
FaqI GGGAC 2 cut(s) 322, 376
FatI CATG 4 cut(s) 137, 544, 576, 645
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 5 cut(s) 43, 100, 379, 382, 482
Fsp4HI GCNGC 5 cut(s) 43, 100, 379, 382, 482
FspBI CTAG 1 cut(s) 679
GlaI GCGC 2 cut(s) 327, 499
GluI GCNGC 5 cut(s) 43, 100, 379, 382, 482
HaeIII GGCC 1 cut(s) 102
HapII CCGG 3 cut(s) 485, 501, 519
HgaI GACGC 1 cut(s) 100
HhaI GCGC 2 cut(s) 328, 500
Hin1II CATG 4 cut(s) 141, 548, 580, 649
Hin6I GCGC 2 cut(s) 326, 498
HinP1I GCGC 2 cut(s) 326, 498
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 4 cut(s) 125, 386, 422, 525
HpaII CCGG 3 cut(s) 485, 501, 519
Hpy166II GTNNAC 2 cut(s) 366, 550
Hpy188III TCNNGA 1 cut(s) 519
Hpy8I GTNNAC 2 cut(s) 366, 550
Hpy99I CGWCG 4 cut(s) 80, 98, 125, 248
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 2 cut(s) 27, 459
HpyCH4IV ACGT 4 cut(s) 93, 436, 462, 630
HpyCH4V TGCA 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 323, 352, 570
HpyF3I CTNAG 1 cut(s) 555
HpySE526I ACGT 4 cut(s) 93, 436, 462, 630
Hsp92II CATG 4 cut(s) 141, 548, 580, 649
HspAI GCGC 2 cut(s) 326, 498
Kpn2I TCCGGA 1 cut(s) 518
KroI GCCGGC 1 cut(s) 484
KroNI GCCGGC 1 cut(s) 486
Kzo9I GATC 3 cut(s) 33, 277, 309
LguI GCTCTTC 1 cut(s) 413
LmnI GCTCC 3 cut(s) 90, 561, 660
LpnPI CCDG 6 cut(s) 33, 474, 498, 514, 532, 583
Lsp1109I GCAGC 1 cut(s) 54
MaeI CTAG 1 cut(s) 679
MaeII ACGT 4 cut(s) 93, 436, 462, 630
MaeIII GTNAC 1 cut(s) 608
MalI GATC 3 cut(s) 35, 279, 311
MbiI CCGCTC 1 cut(s) 378
MboI GATC 3 cut(s) 33, 277, 309
MboII GAAGA 5 cut(s) 43, 279, 281, 319, 400
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 1 cut(s) 188
MlyI GAGTC 2 cut(s) 380, 534
MmeI TCCRAC 2 cut(s) 99, 267
MroI TCCGGA 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 484
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 1 cut(s) 162
MspCI CTTAAG 1 cut(s) 161
MspI CCGG 3 cut(s) 485, 501, 519
MvnI CGCG 3 cut(s) 111, 326, 498
MwoI GCNNNNNNNGC 4 cut(s) 108, 323, 352, 570
NaeI GCCGGC 1 cut(s) 486
NdeII GATC 3 cut(s) 33, 277, 309
NgoMIV GCCGGC 1 cut(s) 484
NlaIII CATG 4 cut(s) 141, 548, 580, 649
NlaIV GGNNCC 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 608
NspI RCATGY 3 cut(s) 141, 580, 649
NspV TTCGAA 1 cut(s) 398
PciI ACATGT 3 cut(s) 137, 576, 645
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 395
PdiI GCCGGC 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 125, 422
PkrI GCNGC 5 cut(s) 44, 101, 380, 383, 483
Ple19I CGATCG 1 cut(s) 280
PleI GAGTC 2 cut(s) 380, 533
PpsI GAGTC 2 cut(s) 380, 533
PscI ACATGT 3 cut(s) 137, 576, 645
Psp1406I AACGTT 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 517
PspPI GGNCC 2 cut(s) 4, 516
PstI CTGCAG 1 cut(s) 44
PsuI RGATCY 1 cut(s) 33
PvuI CGATCG 1 cut(s) 280
RsaI GTAC 3 cut(s) 142, 275, 461
RsaNI GTAC 3 cut(s) 141, 274, 460
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 5 cut(s) 43, 100, 379, 382, 482
Sau3AI GATC 3 cut(s) 33, 277, 309
Sau96I GGNCC 2 cut(s) 4, 516
SchI GAGTC 2 cut(s) 380, 534
SduI GDGCHC 1 cut(s) 304
SfcI CTRYAG 1 cut(s) 40
SfuI TTCGAA 1 cut(s) 398
SinI GGWCC 2 cut(s) 4, 516
SmlI CTYRAG 2 cut(s) 161, 471
SmoI CTYRAG 2 cut(s) 161, 471
Sse9I AATT 1 cut(s) 188
SsiI CCGC 7 cut(s) 67, 99, 109, 378, 381, 482, 496
SspMI CTAG 1 cut(s) 679
TaaI ACNGT 2 cut(s) 27, 459
TaiI ACGT 4 cut(s) 96, 439, 465, 633
TaqI TCGA 5 cut(s) 123, 267, 280, 398, 416
TaqII GACCGA 1 cut(s) 434
TasI AATT 1 cut(s) 188
TatI WGTACW 1 cut(s) 140
TauI GCSGC 4 cut(s) 102, 381, 384, 484
TfiI GAWTC 2 cut(s) 125, 422
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 2 cut(s) 373, 644
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 1 cut(s) 578
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 2 cut(s) 373, 644
Vha464I CTTAAG 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 4, 516
XceI RCATGY 3 cut(s) 141, 580, 649
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.