Rh7AG128200

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006816
Physical Location & Seq
Reverse (-)
10230040 .. 10233458
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG128200.1

Sequence Viewer

Length: 603 bp
ATGGTAGCTGGCAACCAAAACAAAACCGCCACCGAAACCCTAAAATTCCTCTGCAGCTACGGCGGCAAGATCCTTCTCCGCCACACCGACGGCCAGCTCCGCTACGTAGGCGGCCACACCCGCGTCCTCTCCGTCGATCCCTCCATTTCCTTCTCAGAATTAATGGTGAAGCTCGCTGAGTTCTGCGGCCACTCCGTCACTCTCAGGTGTCAATTGCCGGGCGGCGACTTGGAGACGTTGATTTCGATCAAGTCCGACGAGGATTTGGCGAATCTAGTAGAAGAGTACGATCGTGTTTTTTCTTCGTCTCGTTCCTATAAGATCAGAGCGATTCTATCTCCCCCGAAACCGCTGAAGCAAGTCTCGCCTGCTCAGTCCACAACATCGACCAGCGGCGACTGTTCGCCGTCGAAATCGCTCTACTCCGACTTCGATTCGCCGCCGAAGCGTTACTATTCCCCGATGGCGGTTGGGTTTCAGAGAGGGCCGGGCAAAGTGTGCTACTATCCTTGCCATGTCCAGCGAACTCCCTGCGAGATTCCGTATCAGCATCACCGTTATCACCAGCACCCCCATCAATTTCACGCTCCTCACTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

200

Amino Acids

22.39

Weight (kDa)

8.75

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 30 - 109 9e-19 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 123
AclWI GGATC 2 cut(s) 64, 131
AcoI YGGCCR 3 cut(s) 91, 112, 187
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 466
AluBI AGCT 4 cut(s) 8, 57, 97, 172
AluI AGCT 4 cut(s) 8, 57, 97, 172
Alw26I GTCTC 3 cut(s) 227, 312, 367
AlwI GGATC 2 cut(s) 64, 131
AoxI GGCC 4 cut(s) 91, 112, 187, 485
ApeKI GCWGC 1 cut(s) 54
ApoI RAATTY 1 cut(s) 44
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AseI ATTAAT 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 485
AsuC2I CCSGG 2 cut(s) 219, 489
AsuHPI GGTGA 3 cut(s) 178, 545, 554
BbvI GCAGC 1 cut(s) 66
BccI CCATC 2 cut(s) 457, 582
BceAI ACGGC 3 cut(s) 76, 106, 391
BcgI CGANNNNNNTGC 2 cut(s) 513, 547
BcnI CCSGG 2 cut(s) 219, 489
BcoDI GTCTC 3 cut(s) 227, 312, 367
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 394, 440
BlsI GCNGC 7 cut(s) 56, 65, 113, 188, 224, 395, 441
Bme1390I CCNGG 2 cut(s) 219, 489
BmgT120I GGNCC 1 cut(s) 485
BmrFI CCNGG 2 cut(s) 219, 489
BmsI GCATC 1 cut(s) 559
BpuMI CCSGG 2 cut(s) 219, 489
BsaAI YACGTR 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse8I GATNNNNATC 1 cut(s) 245
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 4 cut(s) 168, 168, 217, 386
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 4 cut(s) 93, 114, 189, 487
BsiEI CGRYCG 1 cut(s) 292
BsiSI CCGG 2 cut(s) 218, 488
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 3 cut(s) 227, 312, 367
BsmBI CGTCTC 2 cut(s) 227, 312
BsnI GGCC 4 cut(s) 93, 114, 189, 487
Bsp143I GATC 5 cut(s) 69, 136, 246, 289, 321
BspANI GGCC 4 cut(s) 93, 114, 189, 487
BspCNI CTCAG 4 cut(s) 167, 169, 216, 385
BspFNI CGCG 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 64, 131
BssMI GATC 5 cut(s) 69, 136, 246, 289, 321
Bst4CI ACNGT 2 cut(s) 401, 557
Bst6I CTCTTC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 498
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 4 cut(s) 10, 95, 174, 369
BstDEI CTNAG 4 cut(s) 154, 177, 203, 372
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 5 cut(s) 72, 139, 249, 292, 324
BstMAI GTCTC 3 cut(s) 227, 312, 367
BstMBI GATC 5 cut(s) 69, 136, 246, 289, 321
BstMCI CGRYCG 1 cut(s) 292
BstMWI GCNNNNNNNGC 8 cut(s) 60, 63, 99, 108, 120, 364, 445, 498
BstSCI CCNGG 2 cut(s) 217, 487
BstSFI CTRYAG 1 cut(s) 52
BstSNI TACGTA 1 cut(s) 106
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 4 cut(s) 93, 114, 189, 487
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 4 cut(s) 10, 95, 174, 369
Cfr13I GGNCC 1 cut(s) 485
CseI GACGC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 286
CviAII CATG 1 cut(s) 515
CviJI RGCY 8 cut(s) 8, 57, 93, 97, 114, 172, 189, 487
CviKI_1 RGCY 8 cut(s) 8, 57, 93, 97, 114, 172, 189, 487
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 4 cut(s) 154, 177, 203, 372
DpnI GATC 5 cut(s) 71, 138, 248, 291, 323
DpnII GATC 5 cut(s) 69, 136, 246, 289, 321
EaeI YGGCCR 3 cut(s) 91, 112, 187
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 68
Eco105I TACGTA 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 374
Esp3I CGTCTC 2 cut(s) 227, 312
FaeI CATG 1 cut(s) 518
FaiI YATR 2 cut(s) 318, 516
FatI CATG 1 cut(s) 514
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 394, 440
Fsp4HI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 394, 440
FspBI CTAG 1 cut(s) 275
GluI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 394, 440
HaeIII GGCC 4 cut(s) 93, 114, 189, 487
HapII CCGG 2 cut(s) 218, 488
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 518
HinfI GANTC 4 cut(s) 271, 331, 434, 538
HpaII CCGG 2 cut(s) 218, 488
HphI GGTGA 3 cut(s) 178, 545, 554
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 5 cut(s) 157, 256, 326, 427, 480
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 4 cut(s) 92, 137, 260, 412
HpyAV CCTTC 2 cut(s) 83, 160
HpyCH4III ACNGT 2 cut(s) 401, 557
HpyCH4IV ACGT 2 cut(s) 105, 236
HpyCH4V TGCA 2 cut(s) 54, 597
HpyF10VI GCNNNNNNNGC 8 cut(s) 60, 63, 99, 108, 120, 364, 445, 498
HpyF3I CTNAG 4 cut(s) 154, 177, 203, 372
HpySE526I ACGT 2 cut(s) 105, 236
Hsp92II CATG 1 cut(s) 518
Kzo9I GATC 5 cut(s) 69, 136, 246, 289, 321
LmnI GCTCC 2 cut(s) 102, 592
LpnPI CCDG 9 cut(s) 107, 190, 231, 381, 403, 501, 533, 544, 578
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 559
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 105, 236
MaeIII GTNAC 2 cut(s) 196, 449
MalI GATC 5 cut(s) 71, 138, 248, 291, 323
MboI GATC 5 cut(s) 69, 136, 246, 289, 321
MboII GAAGA 2 cut(s) 293, 294
MfeI CAATTG 2 cut(s) 212, 598
MflI RGATCY 1 cut(s) 69
MluCI AATT 5 cut(s) 44, 158, 212, 578, 598
MmeI TCCRAC 2 cut(s) 279, 450
MnlI CCTC 6 cut(s) 59, 137, 151, 253, 476, 600
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 352, 393
MspI CCGG 2 cut(s) 218, 488
MspR9I CCNGG 2 cut(s) 219, 489
MunI CAATTG 2 cut(s) 212, 598
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 8 cut(s) 60, 63, 99, 108, 120, 364, 445, 498
NciI CCSGG 2 cut(s) 219, 489
NdeII GATC 5 cut(s) 69, 136, 246, 289, 321
NlaIII CATG 1 cut(s) 518
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 4 cut(s) 271, 331, 434, 538
PkrI GCNGC 7 cut(s) 56, 65, 113, 188, 224, 395, 441
Ple19I CGATCG 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 161
PspPI GGNCC 1 cut(s) 485
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 69
PvuI CGATCG 1 cut(s) 292
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 7 cut(s) 55, 64, 112, 187, 223, 394, 440
Sau3AI GATC 5 cut(s) 69, 136, 246, 289, 321
Sau96I GGNCC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 219, 489
SetI ASST 7 cut(s) 10, 59, 99, 108, 174, 209, 239
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 1 cut(s) 52
SnaBI TACGTA 1 cut(s) 106
Sse9I AATT 5 cut(s) 44, 158, 212, 578, 598
SspMI CTAG 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 217, 487
TaaI ACNGT 2 cut(s) 401, 557
TaiI ACGT 2 cut(s) 108, 239
TaqI TCGA 5 cut(s) 135, 245, 386, 410, 432
TasI AATT 5 cut(s) 44, 158, 212, 578, 598
TauI GCSGC 6 cut(s) 66, 114, 189, 225, 396, 442
TfiI GAWTC 4 cut(s) 271, 331, 434, 538
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 599
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 3 cut(s) 121, 184, 531
TspRI CASTG 1 cut(s) 599
VspI ATTAAT 1 cut(s) 161
XapI RAATTY 1 cut(s) 44
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.