Rroxscaffold_3G00262830

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006816
Physical Location & Seq
Forward (+)
56788162 .. 56790862
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262830.1

Sequence Viewer

Length: 603 bp
ATGGTAGCTGGTAACCAAAACAAAACCGCCACCGAAACCCTAAAGTTCCTCTGCAGCTACGGCGGCAAGATCCTCCTCCGCCACACCGACGGCCAGCTCCGCTACGTCGGCGGCCATACCCGCGTCCTCTCCGTCGATCCCTCCATTTCCTTCTCTGAATTAATGGTGAAGCTCGCTGAGTTCTGCGGCCACTCCGTCACTCTCAGGTGTCAATTGCCGGACGGCGACTTGGAGACGTTGATTACGATCAAGTCCGACGAGGATTTGGCGAATCTAATAGAAGAGTACGATCGTGTTTTTTCTTCGACTCGTTCCTATAAGATCAGAGCGATTCTATCTCCCCCGAAACCGCTGAAGCAAGTCTCGCCTGCTCAGTCCACAACATCGACCAGCGGCGACTGTTCGCCGTCGAAATCGCTCTACTCCGACTTCGATTCGCCGCCGAAGCGGTACTATTCCCCGACGGCGGTCGGGTTTCAGAGAGGGCCGGGCAAGGTGTGCTACTATCCTTGCCATGTCCAGCGAACTCCCTGTGAGATTCCGTATCAGCATCACCGTTATCACCAGCACCCTCATCAATTTCACGCTCCTCGCTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

200

Amino Acids

22.48

Weight (kDa)

8.75

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 30 - 109 1.3e-19 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 123
AclWI GGATC 2 cut(s) 64, 131
AcoI YGGCCR 3 cut(s) 91, 112, 187
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 2 cut(s) 287, 452
AfiI CCNNNNNNNGG 1 cut(s) 466
AluBI AGCT 4 cut(s) 8, 57, 97, 172
AluI AGCT 4 cut(s) 8, 57, 97, 172
Alw26I GTCTC 2 cut(s) 227, 367
AlwI GGATC 2 cut(s) 64, 131
AoxI GGCC 4 cut(s) 91, 112, 187, 485
ApeKI GCWGC 2 cut(s) 54, 594
AseI ATTAAT 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 485
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 3 cut(s) 178, 545, 554
BbvI GCAGC 2 cut(s) 66, 581
BceAI ACGGC 5 cut(s) 76, 106, 238, 391, 480
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 2 cut(s) 227, 367
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 7 cut(s) 55, 64, 112, 187, 394, 440, 595
BlsI GCNGC 7 cut(s) 56, 65, 113, 188, 395, 441, 596
Bme1390I CCNGG 1 cut(s) 489
BmgT120I GGNCC 1 cut(s) 485
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 559
BoxI GACNNNNGTC 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse8I GATNNNNATC 1 cut(s) 245
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 168, 217, 386
BseRI GAGGAG 2 cut(s) 65, 579
BseXI GCAGC 2 cut(s) 66, 581
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 2 cut(s) 292, 471
BshFI GGCC 4 cut(s) 93, 114, 189, 487
BsiEI CGRYCG 2 cut(s) 292, 471
BsiSI CCGG 2 cut(s) 218, 488
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 227, 367
BsmBI CGTCTC 1 cut(s) 227
BsnI GGCC 4 cut(s) 93, 114, 189, 487
Bsp143I GATC 5 cut(s) 69, 136, 246, 289, 321
BspANI GGCC 4 cut(s) 93, 114, 189, 487
BspCNI CTCAG 3 cut(s) 169, 216, 385
BspFNI CGCG 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 64, 131
BssMI GATC 5 cut(s) 69, 136, 246, 289, 321
Bst4CI ACNGT 2 cut(s) 401, 557
Bst6I CTCTTC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 95, 174, 369
BstDEI CTNAG 3 cut(s) 177, 203, 372
BstEII GGTNACC 1 cut(s) 11
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 5 cut(s) 72, 139, 249, 292, 324
BstMAI GTCTC 2 cut(s) 227, 367
BstMBI GATC 5 cut(s) 69, 136, 246, 289, 321
BstMCI CGRYCG 2 cut(s) 292, 471
BstMWI GCNNNNNNNGC 8 cut(s) 60, 63, 99, 108, 120, 364, 445, 498
BstPAI GACNNNNGTC 1 cut(s) 467
BstPI GGTNACC 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 52
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 66, 581
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 4 cut(s) 93, 114, 189, 487
Cac8I GCNNGC 3 cut(s) 95, 174, 369
Cfr13I GGNCC 1 cut(s) 485
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 286, 451
CviAII CATG 1 cut(s) 515
CviJI RGCY 8 cut(s) 8, 57, 93, 97, 114, 172, 189, 487
CviKI_1 RGCY 8 cut(s) 8, 57, 93, 97, 114, 172, 189, 487
CviQI GTAC 2 cut(s) 286, 451
DdeI CTNAG 3 cut(s) 177, 203, 372
DpnI GATC 5 cut(s) 71, 138, 248, 291, 323
DpnII GATC 5 cut(s) 69, 136, 246, 289, 321
EaeI YGGCCR 3 cut(s) 91, 112, 187
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 374
Eco91I GGTNACC 1 cut(s) 11
EcoO65I GGTNACC 1 cut(s) 11
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 1 cut(s) 518
FaiI YATR 3 cut(s) 117, 318, 516
FatI CATG 1 cut(s) 514
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 7 cut(s) 55, 64, 112, 187, 394, 440, 595
Fsp4HI GCNGC 7 cut(s) 55, 64, 112, 187, 394, 440, 595
GluI GCNGC 7 cut(s) 55, 64, 112, 187, 394, 440, 595
HaeIII GGCC 4 cut(s) 93, 114, 189, 487
HapII CCGG 2 cut(s) 218, 488
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 518
HinfI GANTC 5 cut(s) 271, 307, 331, 434, 538
HpaII CCGG 2 cut(s) 218, 488
HphI GGTGA 3 cut(s) 178, 545, 554
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 5 cut(s) 157, 256, 326, 427, 480
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 6 cut(s) 92, 110, 137, 260, 412, 466
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 401, 557
HpyCH4IV ACGT 2 cut(s) 105, 236
HpyCH4V TGCA 2 cut(s) 54, 597
HpyF10VI GCNNNNNNNGC 8 cut(s) 60, 63, 99, 108, 120, 364, 445, 498
HpyF3I CTNAG 3 cut(s) 177, 203, 372
HpySE526I ACGT 2 cut(s) 105, 236
Hsp92II CATG 1 cut(s) 518
Kzo9I GATC 5 cut(s) 69, 136, 246, 289, 321
LmnI GCTCC 2 cut(s) 102, 592
LpnPI CCDG 9 cut(s) 107, 190, 231, 381, 403, 501, 533, 544, 578
Lsp1109I GCAGC 2 cut(s) 66, 581
LweI GCATC 1 cut(s) 559
MaeII ACGT 2 cut(s) 105, 236
MaeIII GTNAC 2 cut(s) 11, 196
MalI GATC 5 cut(s) 71, 138, 248, 291, 323
MboI GATC 5 cut(s) 69, 136, 246, 289, 321
MboII GAAGA 2 cut(s) 293, 294
MfeI CAATTG 2 cut(s) 212, 598
MflI RGATCY 1 cut(s) 69
MluCI AATT 4 cut(s) 158, 212, 578, 598
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 2 cut(s) 279, 450
MnlI CCTC 9 cut(s) 59, 83, 86, 137, 151, 253, 476, 582, 600
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 352, 393
MspI CCGG 2 cut(s) 218, 488
MspR9I CCNGG 1 cut(s) 489
MunI CAATTG 2 cut(s) 212, 598
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 8 cut(s) 60, 63, 99, 108, 120, 364, 445, 498
NciI CCSGG 1 cut(s) 489
NdeII GATC 5 cut(s) 69, 136, 246, 289, 321
NlaIII CATG 1 cut(s) 518
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 4 cut(s) 271, 331, 434, 538
PkrI GCNGC 7 cut(s) 56, 65, 113, 188, 395, 441, 596
Ple19I CGATCG 1 cut(s) 292
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PshAI GACNNNNGTC 1 cut(s) 467
PshBI ATTAAT 1 cut(s) 161
PspEI GGTNACC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 485
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 69
PvuI CGATCG 1 cut(s) 292
RsaI GTAC 2 cut(s) 287, 452
RsaNI GTAC 2 cut(s) 286, 451
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 7 cut(s) 55, 64, 112, 187, 394, 440, 595
Sau3AI GATC 5 cut(s) 69, 136, 246, 289, 321
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 489
SetI ASST 8 cut(s) 10, 59, 99, 108, 174, 209, 239, 498
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 1 cut(s) 52
Sse9I AATT 4 cut(s) 158, 212, 578, 598
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 2 cut(s) 401, 557
TaiI ACGT 2 cut(s) 108, 239
TaqI TCGA 5 cut(s) 135, 305, 386, 410, 432
TasI AATT 4 cut(s) 158, 212, 578, 598
TauI GCSGC 5 cut(s) 66, 114, 189, 396, 442
TfiI GAWTC 4 cut(s) 271, 331, 434, 538
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 54, 594
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 3 cut(s) 121, 184, 531
VspI ATTAAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.