Rorug07G0003100

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006816
Physical Location & Seq
Forward (+)
296351 .. 296671
321 bp
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UTR
Exon/CDS
Intron
Rorug07G0003100.1

Sequence Viewer

Length: 321 bp
ATGGGTTCTAGAGGAGGAGCGACGGTTTTGCAGCAGCTAAGTCGGGCGAGCCGAATGAGAACGAAACTGCAGACGGCGTTGGAAGCCAGCGCTGTGGACATAGAAGACGTGTCACATCAGCACGCCGGACACGCCGCCATGAGGGATAACAACGCCGGCGAGACTCATTTCAACCTGAAGATCGTGTCCCCCAAATTCGAGGGGCAGAGCCTCGTTAAACGACACCGCATGGTGTATGATGCCTTGGCCGACGAGCTCCAGTCCGGTCTTCATGCGCTCTCCATTGTCGCCAAGACTCCCCAAGAAACCGGCCCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

106

Amino Acids

11.5

Weight (kDa)

9.22

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 23 - 102 4.6e-32 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 135, 226
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 197
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 141
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 1 cut(s) 172
AjiI CACGTC 1 cut(s) 109
AluBI AGCT 2 cut(s) 37, 256
AluI AGCT 2 cut(s) 37, 256
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 155
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 2 cut(s) 246, 310
ApeKI GCWGC 2 cut(s) 31, 34
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 2 cut(s) 92, 277
AspS9I GGNCC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 2 cut(s) 111, 260
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 43, 46
BceAI ACGGC 1 cut(s) 90
BcgI CGANNNNNNTGC 4 cut(s) 10, 23, 44, 57
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 93
BisI GCNGC 3 cut(s) 32, 35, 135
BlsI GCNGC 3 cut(s) 33, 36, 136
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 2 cut(s) 111, 260
BpmI CTGGAG 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 243
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse118I RCCGGY 2 cut(s) 155, 308
Bse1I ACTGG 1 cut(s) 259
BseDI CCNNGG 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 2 cut(s) 27, 30
BseXI GCAGC 2 cut(s) 43, 46
BshFI GGCC 2 cut(s) 248, 312
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 4 cut(s) 126, 156, 264, 309
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 2 cut(s) 248, 312
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 2 cut(s) 135, 226
BspANI GGCC 2 cut(s) 248, 312
BspLI GGNNCC 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 72
BsrFI RCCGGY 2 cut(s) 155, 308
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 2 cut(s) 155, 308
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 25
BstC8I GCNNGC 4 cut(s) 49, 88, 123, 157
BstDEI CTNAG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 277
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 83, 131
BstSFI CTRYAG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 43, 46
BstV2I GAAGAC 2 cut(s) 111, 260
BstXI CCANNNNNNTGG 1 cut(s) 94
BsuRI GGCC 2 cut(s) 248, 312
BtrI CACGTC 1 cut(s) 109
Cac8I GCNNGC 4 cut(s) 49, 88, 123, 157
CfoI GCGC 2 cut(s) 92, 277
Cfr10I RCCGGY 2 cut(s) 155, 308
Cfr13I GGNCC 1 cut(s) 311
CviAII CATG 3 cut(s) 139, 229, 272
CviJI RGCY 7 cut(s) 37, 51, 86, 210, 248, 256, 312
CviKI_1 RGCY 7 cut(s) 37, 51, 86, 210, 248, 256, 312
DdeI CTNAG 1 cut(s) 38
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 258
Eco47III AGCGCT 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 3 cut(s) 142, 232, 275
FaiI YATR 5 cut(s) 101, 140, 230, 237, 273
FaqI GGGAC 1 cut(s) 172
FatI CATG 3 cut(s) 138, 228, 271
Fnu4HI GCNGC 3 cut(s) 32, 35, 135
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 3 cut(s) 32, 35, 135
FspBI CTAG 1 cut(s) 9
GlaI GCGC 2 cut(s) 91, 276
GluI GCNGC 3 cut(s) 32, 35, 135
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 248, 312
HapII CCGG 4 cut(s) 126, 156, 264, 309
HhaI GCGC 2 cut(s) 92, 277
Hin1II CATG 3 cut(s) 142, 232, 275
Hin6I GCGC 2 cut(s) 90, 275
HinP1I GCGC 2 cut(s) 90, 275
HinfI GANTC 2 cut(s) 163, 295
HpaII CCGG 4 cut(s) 126, 156, 264, 309
Hpy166II GTNNAC 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 2 cut(s) 25, 254
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 31, 70
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 131
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 142, 232, 275
HspAI GCGC 2 cut(s) 90, 275
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 17, 261
LpnPI CCDG 6 cut(s) 100, 139, 169, 188, 272, 277
Lsp1109I GCAGC 2 cut(s) 43, 46
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 111
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 3 cut(s) 116, 190, 260
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 1 cut(s) 194
MlyI GAGTC 2 cut(s) 157, 289
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 5 cut(s) 5, 8, 135, 193, 221
MreI CGCCGGCG 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 155
MseI TTAA 1 cut(s) 216
MspI CCGG 4 cut(s) 126, 156, 264, 309
MwoI GCNNNNNNNGC 2 cut(s) 83, 131
NaeI GCCGGC 1 cut(s) 157
NdeII GATC 1 cut(s) 180
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 3 cut(s) 142, 232, 275
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PdiI GCCGGC 1 cut(s) 157
PkrI GCNGC 3 cut(s) 33, 36, 136
PleI GAGTC 2 cut(s) 157, 289
PpsI GAGTC 2 cut(s) 157, 289
Psp124BI GAGCTC 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 1 cut(s) 311
PstI CTGCAG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 3 cut(s) 32, 35, 135
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 2 cut(s) 157, 289
SduI GDGCHC 1 cut(s) 258
SetI ASST 4 cut(s) 39, 111, 177, 258
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 1 cut(s) 68
SgrAI CRCCGGYG 1 cut(s) 155
Sse9I AATT 1 cut(s) 194
SsiI CCGC 2 cut(s) 135, 226
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 258
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 198
TasI AATT 1 cut(s) 194
TauI GCSGC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 2 cut(s) 31, 34
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 1 cut(s) 260
XapI RAATTY 1 cut(s) 194
XbaI TCTAGA 1 cut(s) 8
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.