MD06G1166200.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006816
Physical Location & Seq
Reverse (-)
30616205 .. 30617827
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1166200.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGGCCTCAACAAAGCACCTGAACCGTTAAAACTCCTCTGCAGCTATGGCGGAAAAATCCTCCCTCGCCATTCCGACGGCACGCTCCGGTACGTCGGCGGCCACAATCGCGTCCTCTCCGTCGATTCATCCATTACGTATACAGAGCTAATGGTGAAGCTTGCGGAGTTGTGTGGCTATTCTGTTGAATTGCGGTGTCCGTTGCCGAATGGAGACTTGGAGACCTTGATTTCCGTCAAGTCCGACGAGGATTTGGCGAATATCATAGAAGAGTACGGTCGAGCTTCTTCTCCCCCTCATTCTCTGAAGATCAGAGCCATTCTCTCGCCGCCGAAGTCCCTGAAGCAAATCTCGCCTCCGATGTCTACTGCTACGAGCGGCGGCGACTCGTCCCCTTCGAAATCGCTCTTCTCATCCGCTGATTCGCCTCGAGTTTCGCCCCCGCAACGGTTCGTTTCGCCTCAAGTCTCGCCACCGAGGCGCTACGTTTCGCCGCAAGTCTCGCCACCGAAACGGTACGCTTCGCCGCCTGCTAGATACCCCGCCGGGCATCAGCGAGGGTCCGGACGAGTCTGCTACTATCCTTGCCATCTCCAGCCTCAGAGTCAGAGGAGTGGCCCCTGCGACATGTCGTATGTTCATCATTACCAGCAGTATCGTCACTACCACCATCATCCTCACTACCACCATCATCCTCACGTTCCCCAATGCGACATGTCGTATGGAAGGAGCTTCACACAGAGGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

250

Amino Acids

27.75

Weight (kDa)

9.38

Isoelectric Point (pI)

83.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 29 - 99 2e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 378
AccI GTMKAC 2 cut(s) 140, 365
AccII CGCG 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 100
AcuI CTGAAG 2 cut(s) 326, 362
AfaI GTAC 3 cut(s) 92, 275, 518
AfiI CCNNNNNNNGG 1 cut(s) 447
AflIII ACRYGT 2 cut(s) 627, 714
AgsI TTSAA 1 cut(s) 188
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 5 cut(s) 45, 148, 160, 284, 732
AluI AGCT 5 cut(s) 45, 148, 160, 284, 732
Alw26I GTCTC 4 cut(s) 207, 215, 472, 505
Ama87I CYCGRG 1 cut(s) 429
Aor13HI TCCGGA 1 cut(s) 563
AoxI GGCC 3 cut(s) 4, 100, 616
ApeKI GCWGC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 483
AspS9I GGNCC 3 cut(s) 4, 561, 617
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 1 cut(s) 166
AsuII TTCGAA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 54
BccI CCATC 3 cut(s) 597, 678, 696
BceAI ACGGC 1 cut(s) 94
BcnI CCSGG 1 cut(s) 547
BcoDI GTCTC 4 cut(s) 207, 215, 472, 505
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 1 cut(s) 40
BfoI RGCGCY 1 cut(s) 484
BglI GCCNNNNNGGC 1 cut(s) 478
BisI GCNGC 7 cut(s) 43, 100, 329, 379, 382, 494, 527
BlsI GCNGC 7 cut(s) 44, 101, 330, 380, 383, 495, 528
Bme1390I CCNGG 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 561
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 3 cut(s) 4, 561, 617
BmiI GGNNCC 2 cut(s) 562, 619
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 1 cut(s) 559
BplI GAGNNNNNCTC 2 cut(s) 306, 338
BpmI CTGGAG 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 547
BsaAI YACGTR 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 476
BsaWI WCCGGW 2 cut(s) 87, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 563
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 4 cut(s) 128, 413, 673, 691
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMII CTCAG 1 cut(s) 614
BseRI GAGGAG 2 cut(s) 26, 625
BseXI GCAGC 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 6, 102, 618
BsiEI CGRYCG 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 3 cut(s) 88, 546, 564
BslFI GGGAC 2 cut(s) 322, 376
BslI CCNNNNNNNGG 1 cut(s) 447
BsmAI GTCTC 4 cut(s) 207, 215, 472, 505
BsmFI GGGAC 2 cut(s) 322, 376
BsnI GGCC 3 cut(s) 6, 102, 618
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 429
Bsp119I TTCGAA 1 cut(s) 398
Bsp13I TCCGGA 1 cut(s) 563
Bsp143I GATC 1 cut(s) 309
BspANI GGCC 3 cut(s) 6, 102, 618
BspCNI CTCAG 1 cut(s) 613
BspEI TCCGGA 1 cut(s) 563
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 2 cut(s) 562, 619
BspMAI CTGCAG 1 cut(s) 44
BspQI GCTCTTC 1 cut(s) 413
BspT104I TTCGAA 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 1 cut(s) 309
BssNAI GTATAC 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 141
Bst4CI ACNGT 4 cut(s) 27, 278, 450, 516
Bst6I CTCTTC 2 cut(s) 264, 413
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 83, 162, 531
BstDEI CTNAG 1 cut(s) 600
BstF5I GGATG 4 cut(s) 128, 413, 673, 691
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 1 cut(s) 483
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 4 cut(s) 207, 215, 472, 505
BstMBI GATC 1 cut(s) 309
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 5 cut(s) 48, 108, 352, 478, 502
BstNSI RCATGY 2 cut(s) 631, 718
BstSCI CCNGG 1 cut(s) 545
BstSFI CTRYAG 1 cut(s) 40
BstSNI TACGTA 1 cut(s) 138
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 54
BstZ17I GTATAC 1 cut(s) 141
BsuRI GGCC 3 cut(s) 6, 102, 618
BtsCI GGATG 4 cut(s) 128, 413, 673, 691
Cac8I GCNNGC 3 cut(s) 83, 162, 531
CfoI GCGC 1 cut(s) 483
Cfr13I GGNCC 3 cut(s) 4, 561, 617
CseI GACGC 1 cut(s) 100
Csp6I GTAC 3 cut(s) 91, 274, 517
CviAII CATG 2 cut(s) 628, 715
CviQI GTAC 3 cut(s) 91, 274, 517
DdeI CTNAG 1 cut(s) 600
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 264, 413
EarI CTCTTC 2 cut(s) 264, 413
EciI GGCGGA 1 cut(s) 66
Eco105I TACGTA 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 561
Eco57I CTGAAG 2 cut(s) 326, 362
Eco88I CYCGRG 1 cut(s) 429
FaeI CATG 2 cut(s) 631, 718
FaiI YATR 8 cut(s) 48, 141, 266, 629, 636, 716, 723, 748
FaqI GGGAC 2 cut(s) 322, 376
FatI CATG 2 cut(s) 627, 714
FauI CCCGC 2 cut(s) 450, 550
FblI GTMKAC 2 cut(s) 140, 365
Fnu4HI GCNGC 7 cut(s) 43, 100, 329, 379, 382, 494, 527
FokI GGATG 4 cut(s) 115, 400, 660, 678
Fsp4HI GCNGC 7 cut(s) 43, 100, 329, 379, 382, 494, 527
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 482
GluI GCNGC 7 cut(s) 43, 100, 329, 379, 382, 494, 527
GsuI CTGGAG 1 cut(s) 578
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 3 cut(s) 6, 102, 618
HapII CCGG 3 cut(s) 88, 546, 564
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 483
Hin1II CATG 2 cut(s) 631, 718
Hin6I GCGC 1 cut(s) 481
HinP1I GCGC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 5 cut(s) 125, 386, 422, 570, 604
HpaII CCGG 3 cut(s) 88, 546, 564
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 141, 366
Hpy188I TCNGA 7 cut(s) 76, 244, 306, 314, 360, 603, 609
Hpy188III TCNNGA 1 cut(s) 564
Hpy8I GTNNAC 2 cut(s) 141, 366
Hpy99I CGWCG 4 cut(s) 80, 98, 125, 248
HpyAV CCTTC 2 cut(s) 405, 720
HpyCH4III ACNGT 4 cut(s) 27, 278, 450, 516
HpyCH4IV ACGT 4 cut(s) 93, 137, 486, 699
HpyCH4V TGCA 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 108, 352, 478, 502
HpyF3I CTNAG 1 cut(s) 600
HpySE526I ACGT 4 cut(s) 93, 137, 486, 699
Hsp92II CATG 2 cut(s) 631, 718
HspAI GCGC 1 cut(s) 481
Kpn2I TCCGGA 1 cut(s) 563
Kzo9I GATC 1 cut(s) 309
LguI GCTCTTC 1 cut(s) 413
LmnI GCTCC 2 cut(s) 90, 729
LpnPI CCDG 9 cut(s) 33, 101, 353, 543, 559, 577, 608, 634, 662
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 1 cut(s) 559
MaeI CTAG 1 cut(s) 534
MaeII ACGT 4 cut(s) 93, 137, 486, 699
MaeIII GTNAC 1 cut(s) 659
MalI GATC 1 cut(s) 311
MbiI CCGCTC 1 cut(s) 378
MboI GATC 1 cut(s) 309
MboII GAAGA 4 cut(s) 279, 281, 319, 400
MluCI AATT 1 cut(s) 188
MlyI GAGTC 3 cut(s) 380, 579, 613
MmeI TCCRAC 2 cut(s) 99, 267
MroI TCCGGA 1 cut(s) 563
MseI TTAA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 3 cut(s) 88, 546, 564
MspR9I CCNGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 5 cut(s) 48, 108, 352, 478, 502
NciI CCSGG 1 cut(s) 547
NdeII GATC 1 cut(s) 309
NlaIII CATG 2 cut(s) 631, 718
NlaIV GGNNCC 2 cut(s) 562, 619
NmuCI GTSAC 1 cut(s) 659
NspI RCATGY 2 cut(s) 631, 718
NspV TTCGAA 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 429
PciI ACATGT 2 cut(s) 627, 714
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PfeI GAWTC 2 cut(s) 125, 422
PkrI GCNGC 7 cut(s) 44, 101, 330, 380, 383, 495, 528
PleI GAGTC 3 cut(s) 380, 578, 612
PpsI GAGTC 3 cut(s) 380, 578, 612
Ppu21I YACGTR 1 cut(s) 138
PscI ACATGT 2 cut(s) 627, 714
PspN4I GGNNCC 2 cut(s) 562, 619
PspPI GGNCC 3 cut(s) 4, 561, 617
PspXI VCTCGAGB 1 cut(s) 429
PstI CTGCAG 1 cut(s) 44
RsaI GTAC 3 cut(s) 92, 275, 518
RsaNI GTAC 3 cut(s) 91, 274, 517
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 7 cut(s) 43, 100, 329, 379, 382, 494, 527
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 3 cut(s) 4, 561, 617
SchI GAGTC 3 cut(s) 380, 579, 613
ScrFI CCNGG 1 cut(s) 547
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 1 cut(s) 40
Sfr274I CTCGAG 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 398
SinI GGWCC 1 cut(s) 561
SlaI CTCGAG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 429, 462
SmoI CTYRAG 2 cut(s) 429, 462
SnaBI TACGTA 1 cut(s) 138
Sse9I AATT 1 cut(s) 188
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 545
TaaI ACNGT 4 cut(s) 27, 278, 450, 516
TaiI ACGT 4 cut(s) 96, 140, 489, 702
TaqI TCGA 4 cut(s) 123, 280, 398, 430
TasI AATT 1 cut(s) 188
TauI GCSGC 6 cut(s) 102, 331, 381, 384, 496, 529
TfiI GAWTC 2 cut(s) 125, 422
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 2 cut(s) 117, 629
TspGWI ACGGA 3 cut(s) 109, 189, 223
VpaK11BI GGWCC 1 cut(s) 561
XceI RCATGY 2 cut(s) 631, 718
XhoI CTCGAG 1 cut(s) 429
XmiI GTMKAC 2 cut(s) 140, 365
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.