MD07G1052300.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006816
Physical Location & Seq
Forward (+)
4841846 .. 4842226
381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1052300.v1.1.491

Sequence Viewer

Length: 381 bp
ATGGATGATTTTCCCGAGAAACCCACTTTAGAAATCGAATTTCACGAAAACCCAGTTGCATCAAAGCCCCTTCGCCACTCCGACGGCACGCTCCGATACGTCGGCGGCCACAACCGCGTCCTTGCCGTCGATTCCTCCATTACATGTACAGAGTTAGTGGAGAAGCTTAAGGAGTTCTGTGGCTACTCTGTGGAATTGAAGTGTCCATTGCCGAACGGAGATTTAGAGACCATGATTACCGTAAAGTCCGACGAGGATTTGGCGAATATCATCGAAGAGTACGGTCGAGCTTCTTCTCCGCTTCGTCCTCTGAAGATCAGAGCCATTCTCGCACCGCAGCACCCCGGACTCCACTCGTCACCTCCTATCTCAGCCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

127

Amino Acids

13.86

Weight (kDa)

5.3

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 31 - 99 3e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 117
AciI CCGC 5 cut(s) 105, 115, 299, 335, 375
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 1 cut(s) 332
AfaI GTAC 2 cut(s) 148, 281
AflII CTTAAG 1 cut(s) 167
AflIII ACRYGT 1 cut(s) 143
AgsI TTSAA 1 cut(s) 199
AluBI AGCT 2 cut(s) 166, 290
AluI AGCT 2 cut(s) 166, 290
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 337
ApoI RAATTY 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 351
AvaI CYCGRG 1 cut(s) 14
BbvI GCAGC 1 cut(s) 349
BceAI ACGGC 2 cut(s) 100, 110
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 221
BfrI CTTAAG 1 cut(s) 167
BisI GCNGC 3 cut(s) 106, 338, 375
BlsI GCNGC 3 cut(s) 107, 339, 376
Bme1390I CCNGG 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 312, 344
BpuMI CCSGG 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 10
BseMI GCAATG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 345
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 14
Bsp1407I TGTACA 1 cut(s) 146
Bsp143I GATC 1 cut(s) 315
BspACI CCGC 5 cut(s) 105, 115, 299, 335, 375
BspANI GGCC 1 cut(s) 108
BspFNI CGCG 1 cut(s) 117
BspTI CTTAAG 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 146
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 241, 284
Bst6I CTCTTC 1 cut(s) 270
BstAFI CTTAAG 1 cut(s) 167
BstAUI TGTACA 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 2 cut(s) 370, 378
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 1 cut(s) 318
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 1 cut(s) 315
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 2 cut(s) 114, 329
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 343
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 1 cut(s) 89
CseI GACGC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 147, 280
CviAII CATG 2 cut(s) 144, 232
CviJI RGCY 7 cut(s) 67, 108, 166, 183, 290, 323, 374
CviKI_1 RGCY 7 cut(s) 67, 108, 166, 183, 290, 323, 374
CviQI GTAC 2 cut(s) 147, 280
DdeI CTNAG 2 cut(s) 370, 378
DpnI GATC 1 cut(s) 317
DpnII GATC 1 cut(s) 315
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 14
FaeI CATG 2 cut(s) 147, 235
FaiI YATR 2 cut(s) 145, 233
FatI CATG 2 cut(s) 143, 231
Fnu4HI GCNGC 3 cut(s) 106, 338, 375
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 106, 338, 375
GluI GCNGC 3 cut(s) 106, 338, 375
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 345
HgaI GACGC 1 cut(s) 106
Hin1II CATG 2 cut(s) 147, 235
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 2 cut(s) 131, 348
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 351
Hpy188I TCNGA 5 cut(s) 82, 95, 250, 312, 320
Hpy188III TCNNGA 2 cut(s) 14, 44
Hpy99I CGWCG 4 cut(s) 86, 104, 131, 254
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 241, 284
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 329
HpyF3I CTNAG 2 cut(s) 370, 378
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 2 cut(s) 147, 235
Kzo9I GATC 1 cut(s) 315
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 2 cut(s) 66, 358
Lsp1109I GCAGC 1 cut(s) 349
LweI GCATC 1 cut(s) 68
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 357
MalI GATC 1 cut(s) 317
MboI GATC 1 cut(s) 315
MboII GAAGA 3 cut(s) 285, 287, 325
MluCI AATT 2 cut(s) 38, 194
MlyI GAGTC 1 cut(s) 342
MmeI TCCRAC 2 cut(s) 105, 273
MnlI CCTC 4 cut(s) 145, 247, 318, 372
MseI TTAA 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 167
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 114, 329
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 315
NlaIII CATG 2 cut(s) 147, 235
NmuCI GTSAC 1 cut(s) 357
NspI RCATGY 1 cut(s) 147
PciI ACATGT 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 3 cut(s) 107, 339, 376
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
PscI ACATGT 1 cut(s) 143
RsaI GTAC 2 cut(s) 148, 281
RsaNI GTAC 2 cut(s) 147, 280
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 3 cut(s) 106, 338, 375
Sau3AI GATC 1 cut(s) 315
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 1 cut(s) 345
SetI ASST 4 cut(s) 102, 168, 292, 364
SfaNI GCATC 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 2 cut(s) 38, 194
SsiI CCGC 5 cut(s) 105, 115, 299, 335, 375
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 241, 284
TaiI ACGT 1 cut(s) 102
TaqI TCGA 4 cut(s) 36, 129, 273, 286
TasI AATT 2 cut(s) 38, 194
TatI WGTACW 1 cut(s) 146
TauI GCSGC 2 cut(s) 108, 377
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 1 cut(s) 337
Tsp45I GTSAC 1 cut(s) 357
TspGWI ACGGA 1 cut(s) 231
Vha464I CTTAAG 1 cut(s) 167
XapI RAATTY 1 cut(s) 38
XceI RCATGY 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.