FvH4_5g11700

Belongs to the beta-ketoacyl-ACP synthases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003245
Physical Location & Seq
Reverse (-)
6635737 .. 6639261
3525 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g11700.t2

Sequence Viewer

Length: 1470 bp
ATGGCTGCTGCTACTTCTTGTTCTTCTGGTGTTCTGTTTGGAGCAAGAGAGAATGGAGCTTTTCTGGGACAGTTTGATGGCCTTAGGCAAGTGGAGAAAATGCATATGCCTTTAGCTCCCACCAAGTCTCATGGACTTGTTTCTACTTCAGCGCCAAAATGTAGAACAATTAAGGCCATGGCATCCCCTGCTCTTTCTGCTCCCAAAAGAGAAACGGACCCCAAGAAAAGAACTGTAATAACAGGGCAGGGTCTAGTCTCAGTTTTTGGAAGTGACATTGATACTTTCTACAACAGACTCCTGGAGGGAGAGAGTGGGATCACTTTAATAGACAGATTTGATGCTTCAAGTTTCTCGGTTAGATTCGCTGGACAGATTCGTGATTTCTCTTCTAAAGGCTACATTGATGGGAAGAATGATCGACGTCTTGATGATTGCTGGAGATATGGTATAGTAGCTGGCAAAAGGGCCCTTGAAGATGCCAACCTTGGACCTGAAGCCCTTAAATCTATGGACAAAACAAGGATTGGAGTCATAGTTGGGACAGGTATGGGAGGCTTAACAGCTTTCAGCGCCGGGGTAGAGTCTCTTGTTCAAAAGGGTTTCAAGAAGATTAGTCCATTTTTCATTCCTTACTCCATTACCAACATGGGATCAGCATTGTTAGCAATAGACACAGGGTTAATGGGACCAAATTACTCCATCTCTACTGCTTGTGCCACTGCAAATTACTGCTTCTATGCAGCTGCAAATCACATTAGAAGAGGTGAAGCAGATATCATGGTAGTTGGAGGGACTGAGGCAGCAGTTATGCCTGTTGGTGTTGGAGGTTTCATTGCTTGCCGAGCCTTGTCTCAGAGAAATGATGAACCCCACAGAGCCTCAAGACCATGGGACAAAAATCGTGATGGTTTTGTAATGGGAGAAGGTGCTGGTGTACTGGTTATGGAGAGCTTGGAGAGTGCATTGAAACGAGGAGCGCCTATAATTGCTGAGTACTTGGGAGGCGCTGTAACGTGTGATGCTCATCACATGACTGATCCTAGATCAGATGGACTTGGAGTGTCATCTTGCATAACCAAGAGTTTAGAAGATGCTGGTGTTTCCCCTGAAGAGGTGAACTATGTGAATGCTCATGCAACATCCACCCTAGCAGGAGATTTGGCTGAGTTTAATGCCATCAAAAAGGTTTTTGGGAAGAACACATCCGAGATGAAGATCAATGGAACCAAGTCCATGATTGGACATGGTCTTGGGGCTGCTGGTGGATTGGAAGCCATAGCAACCATTAAAGCAATCAACACTGGCTGGCTTCACCCAACAATTAATCAAGATGACTTGGAGCCTGCTGTTACAATCGACACAGTCCCAAACGTGAAGAAGAAGCATGAGGTTAATGTTGCTATCTCAAACTCATTCGGCTTTGGAGGACACAATTCTGTGGTGGTCTTTGCCCCTTATAACCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212
Pfam Domains
Protein Families
Ketoacyl-synt_C ketoacyl-synt

Protein Analysis

490

Amino Acids

51.68

Weight (kDa)

7.61

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ketoacyl-synt PF00109 77 - 322 1.5e-52 Beta-ketoacyl synthase, N-terminal domain
Ketoacyl-synt_C PF02801 331 - 412 2.5e-20 Beta-ketoacyl synthase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1461
AatII GACGTC 1 cut(s) 427
AclWI GGATC 3 cut(s) 326, 661, 1034
AcuI CTGAAG 3 cut(s) 132, 516, 1131
AcyI GRCGYC 1 cut(s) 424
AfaI GTAC 2 cut(s) 939, 998
AfiI CCNNNNNNNGG 1 cut(s) 1114
AflIII ACRYGT 1 cut(s) 1016
AgsI TTSAA 5 cut(s) 348, 476, 596, 607, 970
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 6 cut(s) 59, 116, 458, 566, 746, 954
AluI AGCT 6 cut(s) 59, 116, 458, 566, 746, 954
Alw26I GTCTC 4 cut(s) 132, 262, 591, 858
AlwI GGATC 3 cut(s) 326, 661, 1034
AoxI GGCC 3 cut(s) 79, 174, 468
ApaI GGGCCC 1 cut(s) 472
ApeKI GCWGC 6 cut(s) 5, 8, 743, 746, 803, 1259
AseI ATTAAT 1 cut(s) 1326
AspLEI GCGC 4 cut(s) 154, 575, 982, 1010
AspS9I GGNCC 5 cut(s) 217, 468, 469, 491, 689
AsuC2I CCSGG 1 cut(s) 577
AsuHPI GGTGA 3 cut(s) 779, 1129, 1307
AvaII GGWCC 3 cut(s) 217, 491, 689
AxyI CCTNAGG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 472
BbvI GCAGC 4 cut(s) 733, 755, 815, 1246
BccI CCATC 6 cut(s) 71, 401, 710, 902, 1046, 1187
BciT130I CCWGG 1 cut(s) 302
BcnI CCSGG 1 cut(s) 577
BcoDI GTCTC 4 cut(s) 132, 262, 591, 858
BfaI CTAG 3 cut(s) 254, 1044, 1151
BfoI RGCGCY 4 cut(s) 155, 576, 983, 1011
BisI GCNGC 6 cut(s) 6, 9, 744, 747, 804, 1260
BlsI GCNGC 6 cut(s) 7, 10, 745, 748, 805, 1261
BmcAI AGTACT 1 cut(s) 998
Bme1390I CCNGG 2 cut(s) 302, 577
Bme18I GGWCC 3 cut(s) 217, 491, 689
BmgT120I GGNCC 5 cut(s) 217, 468, 469, 491, 689
BmiI GGNNCC 5 cut(s) 219, 470, 690, 1228, 1344
BmrFI CCNGG 2 cut(s) 302, 577
BmsI GCATC 5 cut(s) 191, 331, 469, 1012, 1084
BpmI CTGGAG 2 cut(s) 323, 460
BpuEI CTTGAG 1 cut(s) 868
BpuMI CCSGG 1 cut(s) 577
BsaBI GATNNNNATC 2 cut(s) 1026, 1217
BsaHI GRCGYC 1 cut(s) 424
BsaJI CCNNGG 4 cut(s) 177, 487, 576, 890
BsaXI ACNNNNNCTCC 6 cut(s) 433, 463, 522, 552, 996, 1026
Bsc4I CCNNNNNNNGG 1 cut(s) 1114
Bse1I ACTGG 2 cut(s) 945, 1309
Bse21I CCTNAGG 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 834
Bse8I GATNNNNATC 2 cut(s) 1026, 1217
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 4 cut(s) 177, 487, 576, 890
BseGI GGATG 3 cut(s) 182, 1142, 1205
BseJI GATNNNNATC 2 cut(s) 1026, 1217
BseLI CCNNNNNNNGG 1 cut(s) 1114
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 5 cut(s) 273, 789, 869, 984, 1158
BseNI ACTGG 2 cut(s) 945, 1309
BseRI GAGGAG 1 cut(s) 990
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 4 cut(s) 733, 755, 815, 1246
BshFI GGCC 3 cut(s) 81, 176, 470
BsiSI CCGG 1 cut(s) 576
BslFI GGGAC 6 cut(s) 81, 556, 702, 808, 908, 1352
BslI CCNNNNNNNGG 1 cut(s) 1114
BsmAI GTCTC 4 cut(s) 132, 262, 591, 858
BsmFI GGGAC 6 cut(s) 81, 556, 702, 808, 908, 1352
BsmI GAATGC 1 cut(s) 1135
BsnI GGCC 3 cut(s) 81, 176, 470
Bsp120I GGGCCC 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 472
Bsp143I GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
Bsp19I CCATGG 2 cut(s) 177, 890
BspANI GGCC 3 cut(s) 81, 176, 470
BspCNI CTCAG 5 cut(s) 272, 790, 868, 985, 1159
BspLI GGNNCC 5 cut(s) 219, 470, 690, 1228, 1344
BspPI GGATC 3 cut(s) 326, 661, 1034
BsrDI GCAATG 1 cut(s) 834
BsrI ACTGG 2 cut(s) 945, 1309
BssECI CCNNGG 4 cut(s) 177, 487, 576, 890
BssMI GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
BssNI GRCGYC 1 cut(s) 424
BssT1I CCWWGG 3 cut(s) 177, 487, 890
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 3 cut(s) 72, 235, 1366
Bst6I CTCTTC 3 cut(s) 394, 757, 1107
BstACI GRCGYC 1 cut(s) 424
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 4 cut(s) 460, 841, 1310, 1347
BstDEI CTNAG 6 cut(s) 83, 259, 798, 855, 993, 1167
BstDSI CCRYGG 2 cut(s) 177, 890
BstF5I GGATG 3 cut(s) 182, 1142, 1205
BstH2I RGCGCY 4 cut(s) 155, 576, 983, 1011
BstHHI GCGC 4 cut(s) 154, 575, 982, 1010
BstKTI GATC 6 cut(s) 321, 421, 656, 1042, 1049, 1221
BstMAI GTCTC 4 cut(s) 132, 262, 591, 858
BstMBI GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
BstMWI GCNNNNNNNGC 5 cut(s) 188, 197, 572, 665, 845
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 300, 575
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 4 cut(s) 733, 755, 815, 1246
Bsu36I CCTNAGG 1 cut(s) 83
BsuRI GGCC 3 cut(s) 81, 176, 470
BtgI CCRYGG 2 cut(s) 177, 890
BtsCI GGATG 3 cut(s) 182, 1142, 1205
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 720, 1302
Cac8I GCNNGC 4 cut(s) 460, 841, 1310, 1347
CfoI GCGC 4 cut(s) 154, 575, 982, 1010
Cfr13I GGNCC 5 cut(s) 217, 468, 469, 491, 689
Csp6I GTAC 2 cut(s) 938, 997
CviQI GTAC 2 cut(s) 938, 997
DdeI CTNAG 6 cut(s) 83, 259, 798, 855, 993, 1167
DpnI GATC 6 cut(s) 320, 420, 655, 1041, 1048, 1220
DpnII GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
Eam1104I CTCTTC 3 cut(s) 394, 757, 1107
EarI CTCTTC 3 cut(s) 394, 757, 1107
Eco130I CCWWGG 3 cut(s) 177, 487, 890
Eco24I GRGCYC 1 cut(s) 472
Eco32I GATATC 1 cut(s) 778
Eco47I GGWCC 3 cut(s) 217, 491, 689
Eco57I CTGAAG 3 cut(s) 132, 516, 1131
Eco81I CCTNAGG 1 cut(s) 83
EcoO109I RGGNCCY 2 cut(s) 468, 469
EcoRII CCWGG 1 cut(s) 300
EcoRV GATATC 1 cut(s) 778
EcoT14I CCWWGG 3 cut(s) 177, 487, 890
EcoT22I ATGCAT 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 472
ErhI CCWWGG 3 cut(s) 177, 487, 890
FaqI GGGAC 6 cut(s) 81, 556, 702, 808, 908, 1352
FauNDI CATATG 1 cut(s) 105
Fnu4HI GCNGC 6 cut(s) 6, 9, 744, 747, 804, 1260
FokI GGATG 3 cut(s) 169, 1129, 1192
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 6 cut(s) 6, 9, 744, 747, 804, 1260
FspBI CTAG 3 cut(s) 254, 1044, 1151
GlaI GCGC 4 cut(s) 153, 574, 981, 1009
GluI GCNGC 6 cut(s) 6, 9, 744, 747, 804, 1260
GsuI CTGGAG 2 cut(s) 323, 460
HaeII RGCGCY 4 cut(s) 155, 576, 983, 1011
HaeIII GGCC 3 cut(s) 81, 176, 470
HapII CCGG 1 cut(s) 576
HhaI GCGC 4 cut(s) 154, 575, 982, 1010
Hin1I GRCGYC 1 cut(s) 424
Hin6I GCGC 4 cut(s) 152, 573, 980, 1008
HinP1I GCGC 4 cut(s) 152, 573, 980, 1008
HinfI GANTC 5 cut(s) 297, 363, 376, 531, 584
HpaII CCGG 1 cut(s) 576
HphI GGTGA 3 cut(s) 779, 1129, 1307
Hpy166II GTNNAC 2 cut(s) 938, 1120
Hpy188I TCNGA 3 cut(s) 858, 1051, 1210
Hpy188III TCNNGA 6 cut(s) 380, 428, 607, 885, 905, 1331
Hpy8I GTNNAC 2 cut(s) 938, 1120
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 1 cut(s) 920
HpyCH4III ACNGT 3 cut(s) 72, 235, 1366
HpyCH4IV ACGT 3 cut(s) 424, 1016, 1374
HpyCH4V TGCA 7 cut(s) 103, 725, 743, 749, 965, 1074, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 197, 572, 665, 845
HpyF3I CTNAG 6 cut(s) 83, 259, 798, 855, 993, 1167
HpySE526I ACGT 3 cut(s) 424, 1016, 1374
Hsp92I GRCGYC 1 cut(s) 424
HspAI GCGC 4 cut(s) 152, 573, 980, 1008
Kzo9I GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
LmnI GCTCC 6 cut(s) 41, 56, 121, 205, 977, 1342
Lsp1109I GCAGC 4 cut(s) 733, 755, 815, 1246
LweI GCATC 5 cut(s) 191, 331, 469, 1012, 1084
MaeI CTAG 3 cut(s) 254, 1044, 1151
MaeII ACGT 3 cut(s) 424, 1016, 1374
MaeIII GTNAC 3 cut(s) 272, 1012, 1351
MalI GATC 6 cut(s) 320, 420, 655, 1041, 1048, 1220
MboI GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
MhlI GDGCHC 1 cut(s) 472
MluCI AATT 6 cut(s) 168, 694, 727, 987, 1323, 1435
MlyI GAGTC 3 cut(s) 291, 540, 593
MmeI TCCRAC 2 cut(s) 769, 805
Mph1103I ATGCAT 1 cut(s) 105
MseI TTAA 9 cut(s) 171, 326, 504, 560, 683, 1173, 1290, 1326, 1395
MspA1I CMGCKG 1 cut(s) 746
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 2 cut(s) 302, 577
Mva1269I GAATGC 1 cut(s) 1135
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 5 cut(s) 188, 197, 572, 665, 845
NciI CCSGG 1 cut(s) 577
NcoI CCATGG 2 cut(s) 177, 890
NdeI CATATG 1 cut(s) 105
NdeII GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
NlaIV GGNNCC 5 cut(s) 219, 470, 690, 1228, 1344
NmeAIII GCCGAG 1 cut(s) 869
NmuCI GTSAC 1 cut(s) 272
NsiI ATGCAT 1 cut(s) 105
PctI GAATGC 1 cut(s) 1135
PfeI GAWTC 2 cut(s) 363, 376
PflFI GACNNNGTC 2 cut(s) 1248, 1364
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 6 cut(s) 7, 10, 745, 748, 805, 1261
PleI GAGTC 3 cut(s) 291, 539, 592
PpsI GAGTC 3 cut(s) 291, 539, 592
PshBI ATTAAT 1 cut(s) 1326
PsiI TTATAA 1 cut(s) 1461
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 5 cut(s) 219, 470, 690, 1228, 1344
PspOMI GGGCCC 1 cut(s) 468
PspPI GGNCC 5 cut(s) 217, 468, 469, 491, 689
PsyI GACNNNGTC 2 cut(s) 1248, 1364
PvuII CAGCTG 1 cut(s) 746
RsaI GTAC 2 cut(s) 939, 998
RsaNI GTAC 2 cut(s) 938, 997
SaqAI TTAA 9 cut(s) 171, 326, 504, 560, 683, 1173, 1290, 1326, 1395
SatI GCNGC 6 cut(s) 6, 9, 744, 747, 804, 1260
Sau3AI GATC 6 cut(s) 318, 418, 653, 1039, 1046, 1218
Sau96I GGNCC 5 cut(s) 217, 468, 469, 491, 689
ScaI AGTACT 1 cut(s) 998
SchI GAGTC 3 cut(s) 291, 540, 593
ScrFI CCNGG 2 cut(s) 302, 577
SduI GDGCHC 1 cut(s) 472
SfaNI GCATC 5 cut(s) 191, 331, 469, 1012, 1084
SinI GGWCC 3 cut(s) 217, 491, 689
SmlI CTYRAG 1 cut(s) 883
SmoI CTYRAG 1 cut(s) 883
Sse9I AATT 6 cut(s) 168, 694, 727, 987, 1323, 1435
SspMI CTAG 3 cut(s) 254, 1044, 1151
StyD4I CCNGG 2 cut(s) 300, 575
StyI CCWWGG 3 cut(s) 177, 487, 890
TaaI ACNGT 3 cut(s) 72, 235, 1366
TaiI ACGT 3 cut(s) 427, 1019, 1377
TaqI TCGA 2 cut(s) 421, 1359
TasI AATT 6 cut(s) 168, 694, 727, 987, 1323, 1435
TatI WGTACW 2 cut(s) 937, 996
TfiI GAWTC 2 cut(s) 363, 376
Tru1I TTAA 9 cut(s) 171, 326, 504, 560, 683, 1173, 1290, 1326, 1395
Tru9I TTAA 9 cut(s) 171, 326, 504, 560, 683, 1173, 1290, 1326, 1395
TscAI CASTG 2 cut(s) 727, 1309
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 6 cut(s) 5, 8, 743, 746, 803, 1259
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 4 cut(s) 616, 823, 882, 1229
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 2 cut(s) 727, 1309
Tth111I GACNNNGTC 2 cut(s) 1248, 1364
VpaK11BI GGWCC 3 cut(s) 217, 491, 689
VspI ATTAAT 1 cut(s) 1326
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XspI CTAG 3 cut(s) 254, 1044, 1151
ZraI GACGTC 1 cut(s) 425
ZrmI AGTACT 1 cut(s) 998
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.