FvH4_5g20180

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003766
Physical Location & Seq
Reverse (-)
11906267 .. 11910085
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20180.t3

Sequence Viewer

Length: 711 bp
ATGAGTGCCGGCGGCGGTAATCAGTTGATCTCTGTTCTTCCCGAGGAGCTCCGATTTCAATTTGAGCTGGAAAAACAAAGCTTTTGTGACCTAAAAGTCAGCAACAATACAGAAAACCATGTTGCTTTCAAGGTTAAAACCACTTCCCCTAAGAAGTACTTTGTACGGCCCAACACTGGCATTATACAAGCTTGGGACTCATGTGTCATCAGAGTCACCCTCCAGGCCCAACGAGAATATCCTCCAGACATGCAGTGTAAAGATAAATTTCTCCTACAGAGTACAATAGTGCCTCCAAATTCTGATATTGATGAACTTCCACAAGATACTTTCACCAAGGAAAGTGGAAGGACAGTAGAGGAGTGCAAGCTTAGAGTTGTGTATTTAACTCCTGGTTCAGGTCAAGGAAATGAAGATGGAGCTTCTATGCGTGGTCTTGATACCAATTCTAATCAGACTTTCCAGCGCCTGAAGGAAGAAAGAGATTCAGCTGTTAGACAAACACAGACACTTCAACAAGAACTGGACATACTGAAGAGGCGGAGATACCGGAAAGGTGACCCAAGCTTTTCCTTCACCTTCGCAATTTTCGTGGGACTTATCGGACTCATGGTTGGTTTCCTCTTGAATCTGTCACTGTCTTCACCACCCATAGAAGTTTCACCATCCACAGAATCTTCACCACTAATGGATCCACCATTAATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

237

Amino Acids

26.55

Weight (kDa)

5.42

Isoelectric Point (pI)

56.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cep192_D6 PF22076 10 - 77 5.7e-06 Cep192 domain 6
Motile_Sperm PF00635 11 - 109 9.8e-29 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 95, 203
AciI CCGC 3 cut(s) 12, 15, 541
AclWI GGATC 2 cut(s) 686, 699
AcsI RAATTY 2 cut(s) 266, 298
AcuI CTGAAG 2 cut(s) 491, 554
AfaI GTAC 3 cut(s) 158, 165, 283
AfiI CCNNNNNNNGG 2 cut(s) 176, 398
AgsI TTSAA 4 cut(s) 59, 130, 515, 628
AjnI CCWGG 2 cut(s) 222, 391
AluBI AGCT 8 cut(s) 49, 67, 81, 191, 370, 422, 491, 567
AluI AGCT 8 cut(s) 49, 67, 81, 191, 370, 422, 491, 567
Alw21I GWGCWC 1 cut(s) 51
AlwI GGATC 2 cut(s) 686, 699
AlwNI CAGNNNCTG 1 cut(s) 469
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 2 cut(s) 167, 225
ApoI RAATTY 2 cut(s) 266, 298
AseI ATTAAT 1 cut(s) 701
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 2 cut(s) 168, 226
AsuHPI GGTGA 7 cut(s) 208, 325, 568, 569, 636, 654, 672
AvaI CYCGRG 1 cut(s) 41
BamHI GGATCC 1 cut(s) 691
BanII GRGCYC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 51
BccI CCATC 2 cut(s) 410, 673
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 2 cut(s) 224, 393
BfmI CTRYAG 1 cut(s) 275
BfoI RGCGCY 1 cut(s) 469
BisI GCNGC 1 cut(s) 13
BlsI GCNGC 1 cut(s) 14
BmcAI AGTACT 1 cut(s) 158
Bme1390I CCNGG 2 cut(s) 224, 393
BmeT110I CYCGRG 1 cut(s) 41
BmgT120I GGNCC 2 cut(s) 168, 226
BmiI GGNNCC 1 cut(s) 693
BmrFI CCNGG 2 cut(s) 224, 393
BpiI GAAGAC 1 cut(s) 633
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpmI CTGGAG 2 cut(s) 206, 228
BsaJI CCNNGG 2 cut(s) 42, 336
BsaWI WCCGGW 1 cut(s) 549
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 398
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 2 cut(s) 181, 528
BseBI CCWGG 2 cut(s) 224, 393
BseDI CCNNGG 2 cut(s) 42, 336
BseGI GGATG 1 cut(s) 665
BseLI CCNNNNNNNGG 2 cut(s) 176, 398
BseNI ACTGG 2 cut(s) 181, 528
BseRI GAGGAG 2 cut(s) 59, 374
BshFI GGCC 2 cut(s) 169, 227
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 2 cut(s) 9, 550
BslFI GGGAC 2 cut(s) 209, 609
BslI CCNNNNNNNGG 2 cut(s) 176, 398
BsmFI GGGAC 2 cut(s) 209, 609
BsnI GGCC 2 cut(s) 169, 227
BsoBI CYCGRG 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 2 cut(s) 27, 691
BspACI CCGC 3 cut(s) 12, 15, 541
BspANI GGCC 2 cut(s) 169, 227
BspLI GGNNCC 1 cut(s) 693
BspPI GGATC 2 cut(s) 686, 699
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 2 cut(s) 181, 528
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 2 cut(s) 42, 336
BssMI GATC 2 cut(s) 27, 691
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 2 cut(s) 224, 393
Bst4CI ACNGT 2 cut(s) 355, 639
Bst6I CTCTTC 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 10, 368
BstDEI CTNAG 2 cut(s) 150, 371
BstEII GGTNACC 1 cut(s) 557
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 665
BstH2I RGCGCY 1 cut(s) 469
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 2 cut(s) 30, 694
BstMBI GATC 2 cut(s) 27, 691
BstNI CCWGG 2 cut(s) 224, 393
BstNSI RCATGY 1 cut(s) 253
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 222, 391
BstSFI CTRYAG 1 cut(s) 275
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 691
BstYI RGATCY 1 cut(s) 691
BsuRI GGCC 2 cut(s) 169, 227
BtsCI GGATG 1 cut(s) 665
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 3 cut(s) 174, 260, 635
Cac8I GCNNGC 2 cut(s) 10, 368
CaiI CAGNNNCTG 1 cut(s) 469
CfoI GCGC 1 cut(s) 468
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 2 cut(s) 168, 226
Csp6I GTAC 3 cut(s) 157, 164, 282
CspCI CAANNNNNGTGG 4 cut(s) 325, 360, 573, 608
CviAII CATG 4 cut(s) 119, 201, 250, 610
CviQI GTAC 3 cut(s) 157, 164, 282
DdeI CTNAG 2 cut(s) 150, 371
DpnI GATC 2 cut(s) 29, 693
DpnII GATC 2 cut(s) 27, 691
DrdI GACNNNNNNGTC 2 cut(s) 95, 203
DseDI GACNNNNNNGTC 2 cut(s) 95, 203
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
EciI GGCGGA 1 cut(s) 556
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 491, 554
Eco88I CYCGRG 1 cut(s) 41
Eco91I GGTNACC 1 cut(s) 557
EcoICRI GAGCTC 1 cut(s) 49
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO65I GGTNACC 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 222, 391
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 4 cut(s) 122, 204, 253, 613
FaiI YATR 8 cut(s) 120, 185, 202, 251, 428, 530, 611, 653
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 209, 609
FatI CATG 4 cut(s) 118, 200, 249, 609
Fnu4HI GCNGC 1 cut(s) 13
FokI GGATG 1 cut(s) 652
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 13
GlaI GCGC 1 cut(s) 467
GluI GCNGC 1 cut(s) 13
GsuI CTGGAG 2 cut(s) 206, 228
HaeII RGCGCY 1 cut(s) 469
HaeIII GGCC 2 cut(s) 169, 227
HapII CCGG 2 cut(s) 9, 550
HhaI GCGC 1 cut(s) 468
Hin1II CATG 4 cut(s) 122, 204, 253, 613
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HindIII AAGCTT 4 cut(s) 79, 189, 368, 565
HinfI GANTC 6 cut(s) 197, 213, 485, 606, 628, 674
HpaII CCGG 2 cut(s) 9, 550
HphI GGTGA 7 cut(s) 208, 325, 568, 569, 636, 654, 672
Hpy188I TCNGA 5 cut(s) 53, 212, 304, 456, 605
Hpy188III TCNNGA 4 cut(s) 41, 245, 437, 625
HpyAV CCTTC 4 cut(s) 342, 466, 583, 589
HpyCH4III ACNGT 2 cut(s) 355, 639
HpyCH4V TGCA 2 cut(s) 253, 366
HpyF3I CTNAG 2 cut(s) 150, 371
Hsp92II CATG 4 cut(s) 122, 204, 253, 613
HspAI GCGC 1 cut(s) 466
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
Kzo9I GATC 2 cut(s) 27, 691
LmnI GCTCC 3 cut(s) 46, 54, 419
MaeIII GTNAC 4 cut(s) 86, 214, 557, 633
MalI GATC 2 cut(s) 29, 693
MboI GATC 2 cut(s) 27, 691
MboII GAAGA 6 cut(s) 29, 425, 488, 547, 633, 669
MflI RGATCY 1 cut(s) 691
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 5 cut(s) 59, 266, 298, 445, 585
MlyI GAGTC 3 cut(s) 191, 222, 600
MnlI CCTC 7 cut(s) 37, 230, 252, 303, 352, 531, 632
MroNI GCCGGC 1 cut(s) 8
MseI TTAA 3 cut(s) 135, 386, 701
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 2 cut(s) 9, 550
MspR9I CCNGG 2 cut(s) 224, 393
MvaI CCWGG 2 cut(s) 224, 393
NaeI GCCGGC 1 cut(s) 10
NdeII GATC 2 cut(s) 27, 691
NgoMIV GCCGGC 1 cut(s) 8
NlaIII CATG 4 cut(s) 122, 204, 253, 613
NlaIV GGNNCC 1 cut(s) 693
NmuCI GTSAC 4 cut(s) 86, 214, 557, 633
NspI RCATGY 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PdiI GCCGGC 1 cut(s) 10
PfeI GAWTC 3 cut(s) 485, 628, 674
PkrI GCNGC 1 cut(s) 14
PleI GAGTC 3 cut(s) 191, 221, 600
PpsI GAGTC 3 cut(s) 191, 221, 600
PshBI ATTAAT 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 222, 391
PspEI GGTNACC 1 cut(s) 557
PspGI CCWGG 2 cut(s) 222, 391
PspN4I GGNNCC 1 cut(s) 693
PspPI GGNCC 2 cut(s) 168, 226
PsrI GAACNNNNNNTAC 2 cut(s) 513, 545
PstNI CAGNNNCTG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 691
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 3 cut(s) 158, 165, 283
RsaNI GTAC 3 cut(s) 157, 164, 282
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 3 cut(s) 135, 386, 701
SatI GCNGC 1 cut(s) 13
Sau3AI GATC 2 cut(s) 27, 691
Sau96I GGNCC 2 cut(s) 168, 226
ScaI AGTACT 1 cut(s) 158
SchI GAGTC 3 cut(s) 191, 222, 600
ScrFI CCNGG 2 cut(s) 224, 393
SduI GDGCHC 1 cut(s) 51
SfcI CTRYAG 1 cut(s) 275
Sse9I AATT 5 cut(s) 59, 266, 298, 445, 585
SsiI CCGC 3 cut(s) 12, 15, 541
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 222, 391
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 355, 639
TasI AATT 5 cut(s) 59, 266, 298, 445, 585
TatI WGTACW 2 cut(s) 156, 281
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 3 cut(s) 485, 628, 674
Tru1I TTAA 3 cut(s) 135, 386, 701
Tru9I TTAA 3 cut(s) 135, 386, 701
TscAI CASTG 3 cut(s) 181, 260, 642
TseFI GTSAC 4 cut(s) 86, 214, 557, 633
Tsp45I GTSAC 4 cut(s) 86, 214, 557, 633
TspDTI ATGAA 2 cut(s) 327, 426
TspRI CASTG 3 cut(s) 181, 260, 642
VspI ATTAAT 1 cut(s) 701
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 2 cut(s) 266, 298
XceI RCATGY 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.