Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003766

Physical Location & Seq

Genomic Coordinates Forward (+)

6057756 .. 6062088

Gene Structure 4333 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1051600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 666 bp

ATGAGTGCCGGTGGTGGTAATCAGTTGATTTCCGTTCATCCTGATGAGCTCAAATTCCTATTTGAGCTGGAAAAGCAAAGCTTTTGTGATCTAAAAGTTGTCAATCACACAGAACACCATGTTGCTTTTAAGGTTAAAACCACTTCACCGAAGAAGTACTTTGTACGGCCCAACACTGGTATTATACAGCCTTGGGATTCATATGTAATCAGAGTCACCCTCCAAGCCCAACGAGAATATCCTCCAGATATGCAGTGCAAAGATAAATTTCTTCTGCTGAGTACAACAGTGCCTCCAAATACTGATGTTGATGACCTTCCACAAGATACTTTCACTAAGGAAACTGGAAGGGTTTTAGAGGAGTGCAAGCTTAGAGTTGTGTATATCACTCCTGCTCAAGGAAACTCAGAAGATGAAGCTATGCAGAGCTCCATGCGTAGTCTTGATACTAATTCTAATCAAGCTTTCCAGCGCCTGAAGGAAGAAAGAGATGCAGCTGTTAGACAAACACAGCAACTTCAACAGGAATTGGATATGCTGAAGAAGCGAAGATTTCGGAAGGGTGACCCAGGCTTCTCCTTCACCTTTGCAATTTTAGTGGGACTGATTGGACTCATGGTTGGCTTCCTCTTGAACCTTTCGTTGTCTTCACCATCTACAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Motile_Sperm

Protein Analysis

222

Amino Acids

25.13

Weight (kDa)

6.45

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Motile_Sperm	PF00635	10 - 109	9.2e-28	MSP (Major sperm protein) domain
Cep192_D6	PF22076	10 - 78	4.3e-07	Cep192 domain 6

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47180 AT5G47180
fragaria_vesca	FvH4_5g20180 FvH4_5g20180 FvH4_5g20180
malus_domestica	MD04G1051600.v1.1 MD06G1048500.v1.1
prunus_persica	Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1
pyrus_communis	pycom04g04530 pycom06g04160
rosa_chinensis	RchiOBHm_Chr7g0205251 RchiOBHm_Chr7g0205261 RchiOBHm_Chr7g0205271
rosa_laevigata	RLG00000003392
rosa_multiflora	Rmu_co8442685.1_g000001 Rmu_sc0013888.1_g000001
rosa_roxburghii	Rroxscaffold_3G00252520 Rroxscaffold_3G00252530
rosa_rugosa	Rorug07G0088800 Rorug07G0088900 Rorug07G0088900 Rorug07G0089000 Rorug07G0089100 Rorug07G0089200
rosa_samantha	Rh7AG219600 Rh7AG219700 Rh7CG232500 Rh7CG232600
rosa_wichuraiana	Rw7G018990 Rw7G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AcsI	RAATTY	2 cut(s)	53, 266
AcuI	CTGAAG	2 cut(s)	497, 560
AfaI	GTAC	3 cut(s)	158, 165, 283
AfiI	CCNNNNNNNGG	2 cut(s)	176, 398
AgsI	TTSAA	2 cut(s)	521, 634
AjnI	CCWGG	1 cut(s)	568
AluBI	AGCT	8 cut(s)	49, 67, 81, 370, 419, 429, 464, 497
AluI	AGCT	8 cut(s)	49, 67, 81, 370, 419, 429, 464, 497
Alw21I	GWGCWC	2 cut(s)	51, 431
AlwNI	CAGNNNCTG	1 cut(s)	475
AoxI	GGCC	1 cut(s)	167
ApeKI	GCWGC	1 cut(s)	494
ApoI	RAATTY	2 cut(s)	53, 266
AspLEI	GCGC	1 cut(s)	474
AspS9I	GGNCC	1 cut(s)	168
AsuHPI	GGTGA	5 cut(s)	138, 208, 574, 575, 642
BanII	GRGCYC	2 cut(s)	51, 431
BbsI	GAAGAC	1 cut(s)	639
Bbv12I	GWGCWC	2 cut(s)	51, 431
BbvI	GCAGC	1 cut(s)	506
BccI	CCATC	1 cut(s)	661
BceAI	ACGGC	1 cut(s)	182
BciT130I	CCWGG	1 cut(s)	570
BfmI	CTRYAG	1 cut(s)	657
BfoI	RGCGCY	1 cut(s)	475
BisI	GCNGC	1 cut(s)	495
BlsI	GCNGC	1 cut(s)	496
BmcAI	AGTACT	1 cut(s)	158
Bme1390I	CCNGG	1 cut(s)	570
BmgT120I	GGNCC	1 cut(s)	168
BmrFI	CCNGG	1 cut(s)	570
BmsI	GCATC	1 cut(s)	481
BpiI	GAAGAC	1 cut(s)	639
BplI	GAGNNNNNCTC	2 cut(s)	204, 236
BpmI	CTGGAG	1 cut(s)	228
BpuEI	CTTGAG	1 cut(s)	381
BsaJI	CCNNGG	2 cut(s)	191, 568
BsaXI	ACNNNNNCTCC	2 cut(s)	277, 307
Bsc4I	CCNNNNNNNGG	2 cut(s)	176, 398
Bse118I	RCCGGY	1 cut(s)	8
Bse1I	ACTGG	2 cut(s)	181, 349
BseBI	CCWGG	1 cut(s)	570
BseDI	CCNNGG	2 cut(s)	191, 568
BseGI	GGATG	1 cut(s)	37
BseLI	CCNNNNNNNGG	2 cut(s)	176, 398
BseMII	CTCAG	2 cut(s)	269, 420
BseNI	ACTGG	2 cut(s)	181, 349
BseRI	GAGGAG	1 cut(s)	374
BseXI	GCAGC	1 cut(s)	506
BshFI	GGCC	1 cut(s)	169
BsiHKAI	GWGCWC	2 cut(s)	51, 431
BsiSI	CCGG	1 cut(s)	9
BslFI	GGGAC	1 cut(s)	615
BslI	CCNNNNNNNGG	2 cut(s)	176, 398
BsmFI	GGGAC	1 cut(s)	615
BsnI	GGCC	1 cut(s)	169
Bsp1286I	GDGCHC	2 cut(s)	51, 431
Bsp143I	GATC	1 cut(s)	88
BspANI	GGCC	1 cut(s)	169
BspCNI	CTCAG	2 cut(s)	270, 419
BsrFI	RCCGGY	1 cut(s)	8
BsrI	ACTGG	2 cut(s)	181, 349
BssAI	RCCGGY	1 cut(s)	8
BssECI	CCNNGG	2 cut(s)	191, 568
BssMI	GATC	1 cut(s)	88
BssT1I	CCWWGG	1 cut(s)	191
Bst2UI	CCWGG	1 cut(s)	570
Bst4CI	ACNGT	1 cut(s)	289
BstC8I	GCNNGC	1 cut(s)	368
BstDEI	CTNAG	4 cut(s)	278, 336, 371, 406
BstEII	GGTNACC	1 cut(s)	563
BstENI	CCTNNNNNAGG	1 cut(s)	396
BstF5I	GGATG	1 cut(s)	37
BstH2I	RGCGCY	1 cut(s)	475
BstHHI	GCGC	1 cut(s)	474
BstKTI	GATC	1 cut(s)	91
BstMBI	GATC	1 cut(s)	88
BstMWI	GCNNNNNNNGC	2 cut(s)	73, 544
BstNI	CCWGG	1 cut(s)	570
BstPI	GGTNACC	1 cut(s)	563
BstSCI	CCNGG	1 cut(s)	568
BstSFI	CTRYAG	1 cut(s)	657
BstV1I	GCAGC	1 cut(s)	506
BstV2I	GAAGAC	1 cut(s)	639
BsuRI	GGCC	1 cut(s)	169
BtsCI	GGATG	1 cut(s)	37
BtsI	GCAGTG	1 cut(s)	260
BtsIMutI	CAGTG	3 cut(s)	174, 260, 294
Cac8I	GCNNGC	1 cut(s)	368
CaiI	CAGNNNCTG	1 cut(s)	475
CfoI	GCGC	1 cut(s)	474
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	1 cut(s)	168
Csp6I	GTAC	3 cut(s)	157, 164, 282
CspCI	CAANNNNNGTGG	2 cut(s)	579, 614
CviAII	CATG	3 cut(s)	119, 433, 616
CviQI	GTAC	3 cut(s)	157, 164, 282
DdeI	CTNAG	4 cut(s)	278, 336, 371, 406
DpnI	GATC	1 cut(s)	90
DpnII	GATC	1 cut(s)	88
Ecl136II	GAGCTC	2 cut(s)	49, 429
Eco130I	CCWWGG	1 cut(s)	191
Eco24I	GRGCYC	2 cut(s)	51, 431
Eco53kI	GAGCTC	2 cut(s)	49, 429
Eco57I	CTGAAG	2 cut(s)	497, 560
Eco91I	GGTNACC	1 cut(s)	563
EcoICRI	GAGCTC	2 cut(s)	49, 429
EcoNI	CCTNNNNNAGG	1 cut(s)	396
EcoO65I	GGTNACC	1 cut(s)	563
EcoRII	CCWGG	1 cut(s)	568
EcoT14I	CCWWGG	1 cut(s)	191
EcoT38I	GRGCYC	2 cut(s)	51, 431
ErhI	CCWWGG	1 cut(s)	191
FaeI	CATG	3 cut(s)	122, 436, 619
FalI	AAGNNNNNCTT	4 cut(s)	65, 97, 143, 175
FaqI	GGGAC	1 cut(s)	615
FatI	CATG	3 cut(s)	118, 432, 615
FauNDI	CATATG	1 cut(s)	202
Fnu4HI	GCNGC	1 cut(s)	495
FokI	GGATG	1 cut(s)	24
FriOI	GRGCYC	2 cut(s)	51, 431
Fsp4HI	GCNGC	1 cut(s)	495
GlaI	GCGC	1 cut(s)	473
GluI	GCNGC	1 cut(s)	495
GsuI	CTGGAG	1 cut(s)	228
HaeII	RGCGCY	1 cut(s)	475
HaeIII	GGCC	1 cut(s)	169
HapII	CCGG	1 cut(s)	9
HhaI	GCGC	1 cut(s)	474
Hin1II	CATG	3 cut(s)	122, 436, 619
Hin6I	GCGC	1 cut(s)	472
HinP1I	GCGC	1 cut(s)	472
HindIII	AAGCTT	3 cut(s)	79, 368, 462
HinfI	GANTC	3 cut(s)	197, 213, 612
HpaII	CCGG	1 cut(s)	9
HphI	GGTGA	5 cut(s)	138, 208, 574, 575, 642
Hpy188I	TCNGA	3 cut(s)	212, 409, 558
Hpy188III	TCNNGA	4 cut(s)	41, 245, 443, 631
HpyAV	CCTTC	5 cut(s)	326, 342, 472, 553, 589
HpyCH4III	ACNGT	1 cut(s)	289
HpyCH4V	TGCA	6 cut(s)	253, 258, 366, 424, 494, 590
HpyF10VI	GCNNNNNNNGC	2 cut(s)	73, 544
HpyF3I	CTNAG	4 cut(s)	278, 336, 371, 406
Hsp92II	CATG	3 cut(s)	122, 436, 619
HspAI	GCGC	1 cut(s)	472
Kzo9I	GATC	1 cut(s)	88
LmnI	GCTCC	1 cut(s)	434
Lsp1109I	GCAGC	1 cut(s)	506
LweI	GCATC	1 cut(s)	481
MaeIII	GTNAC	2 cut(s)	214, 563
MalI	GATC	1 cut(s)	90
MboI	GATC	1 cut(s)	88
MboII	GAAGA	7 cut(s)	163, 263, 422, 494, 553, 561, 639
MhlI	GDGCHC	2 cut(s)	51, 431
MluCI	AATT	5 cut(s)	53, 266, 451, 527, 591
MlyI	GAGTC	2 cut(s)	222, 606
MnlI	CCTC	5 cut(s)	230, 252, 303, 352, 638
MseI	TTAA	2 cut(s)	129, 135
MslI	CAYNNNNRTG	1 cut(s)	42
MspA1I	CMGCKG	1 cut(s)	497
MspI	CCGG	1 cut(s)	9
MspR9I	CCNGG	1 cut(s)	570
MvaI	CCWGG	1 cut(s)	570
MwoI	GCNNNNNNNGC	2 cut(s)	73, 544
NdeI	CATATG	1 cut(s)	202
NdeII	GATC	1 cut(s)	88
NlaIII	CATG	3 cut(s)	122, 436, 619
NmuCI	GTSAC	2 cut(s)	214, 563
PfeI	GAWTC	1 cut(s)	197
PkrI	GCNGC	1 cut(s)	496
PleI	GAGTC	2 cut(s)	221, 606
PpsI	GAGTC	2 cut(s)	221, 606
Psp124BI	GAGCTC	2 cut(s)	51, 431
Psp6I	CCWGG	1 cut(s)	568
PspEI	GGTNACC	1 cut(s)	563
PspGI	CCWGG	1 cut(s)	568
PspPI	GGNCC	1 cut(s)	168
PstNI	CAGNNNCTG	1 cut(s)	475
PvuII	CAGCTG	1 cut(s)	497
RsaI	GTAC	3 cut(s)	158, 165, 283
RsaNI	GTAC	3 cut(s)	157, 164, 282
RseI	CAYNNNNRTG	1 cut(s)	42
SacI	GAGCTC	2 cut(s)	51, 431
SaqAI	TTAA	2 cut(s)	129, 135
SatI	GCNGC	1 cut(s)	495
Sau3AI	GATC	1 cut(s)	88
Sau96I	GGNCC	1 cut(s)	168
ScaI	AGTACT	1 cut(s)	158
SchI	GAGTC	2 cut(s)	222, 606
ScrFI	CCNGG	1 cut(s)	570
SduI	GDGCHC	2 cut(s)	51, 431
SfaNI	GCATC	1 cut(s)	481
SfcI	CTRYAG	1 cut(s)	657
SmiMI	CAYNNNNRTG	1 cut(s)	42
SmlI	CTYRAG	1 cut(s)	396
SmoI	CTYRAG	1 cut(s)	396
Sse9I	AATT	5 cut(s)	53, 266, 451, 527, 591
SstI	GAGCTC	2 cut(s)	51, 431
StyD4I	CCNGG	1 cut(s)	568
StyI	CCWWGG	1 cut(s)	191
TaaI	ACNGT	1 cut(s)	289
TasI	AATT	5 cut(s)	53, 266, 451, 527, 591
TatI	WGTACW	2 cut(s)	156, 281
TfiI	GAWTC	1 cut(s)	197
Tru1I	TTAA	2 cut(s)	129, 135
Tru9I	TTAA	2 cut(s)	129, 135
TscAI	CASTG	3 cut(s)	181, 260, 294
TseFI	GTSAC	2 cut(s)	214, 563
TseI	GCWGC	1 cut(s)	494
Tsp45I	GTSAC	2 cut(s)	214, 563
TspDTI	ATGAA	3 cut(s)	26, 189, 429
TspGWI	ACGGA	1 cut(s)	22
TspRI	CASTG	3 cut(s)	181, 260, 294
XagI	CCTNNNNNAGG	1 cut(s)	396
XapI	RAATTY	2 cut(s)	53, 266
ZrmI	AGTACT	1 cut(s)	158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.