Rroxscaffold_3G00252520

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003766
Physical Location & Seq
Reverse (-)
46546535 .. 46550505
3971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252520.1

Sequence Viewer

Length: 798 bp
ATGAGTGCCGGTGGTGGTAATCAGTTGATCTCTGTTCTTCCGGAGGAGCTCAGATTTCAATTTGAGCTCGAAAAGCAAAGCTTTTGTGATCTAAAAGTCATCAACAACACAGAAAACCATGTTGCTTTCAAGGTTAAAACCACTTCCCCTAAGAAGTACTTTGTACGGCCCAACACTGGTGTTATACAGGCTTGGGACTCATGTGTAATCAGAGGTATCCTAGAACTATGCTGCAAAGCAGATGAGCTCCCCACTGCTTTAGCTAATAAGATACAGAGATTTTATAATTACTGTCTTCCACTAACTGCTGGGTCTGCAGTCACCCTCCAAGCCCAACGAGAATATCCTCCAGACATGCAGTGCAAGGATAAATTTCTCCTACAGAGTACAACGGTGCCTCCAAATTCTGATGTTGATGAACTTCCACAAGATACTTTCACCAAAGAAAGTGGAAGGACAATAGAGGAGTGCAAGCTTAGAGTTGTCTATTTAACTCCAGGTCAAGGAAACTCAGAAGACGGAGCTTCTATGCGTGGTCTTGATACCAATTCTAATCAGGCTTTCCAGCGCCTGAAGGAAGAAAGAGATTCAGCTGTTAGACAAACACAGACACTTCAACAGGAACTGGACATACTGAAGAGGCGGAGATACCGGAAAGGTGACCCAGGCTTTTCCTTCACCTTTGCAATTTTTGTGGGACTGATTGGACTCATGGTTGGCTTCCTCTTGAATCTGTCATTGTCTTCACCACCCATAGAAGCTTCATCATCCACAGAATCTTCACCATTAATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

265

Amino Acids

29.62

Weight (kDa)

5.68

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 11 - 71 2.4e-18 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 394
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 1 cut(s) 643
AcsI RAATTY 2 cut(s) 371, 403
AcuI CTGAAG 2 cut(s) 593, 656
AfaI GTAC 3 cut(s) 158, 165, 388
AfiI CCNNNNNNNGG 2 cut(s) 176, 503
AgsI TTSAA 4 cut(s) 59, 130, 617, 730
AjnI CCWGG 2 cut(s) 496, 664
AluBI AGCT 9 cut(s) 49, 67, 81, 247, 263, 475, 524, 593, 761
AluI AGCT 9 cut(s) 49, 67, 81, 247, 263, 475, 524, 593, 761
Alw21I GWGCWC 3 cut(s) 51, 69, 249
AlwNI CAGNNNCTG 2 cut(s) 571, 625
Aor13HI TCCGGA 1 cut(s) 40
AoxI GGCC 1 cut(s) 167
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 2 cut(s) 371, 403
AseI ATTAAT 1 cut(s) 788
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 6 cut(s) 313, 430, 670, 671, 738, 774
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 51, 69, 249
BbsI GAAGAC 3 cut(s) 287, 522, 735
Bbv12I GWGCWC 3 cut(s) 51, 69, 249
BbvI GCAGC 1 cut(s) 218
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 2 cut(s) 498, 666
BciVI GTATCC 1 cut(s) 227
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 2 cut(s) 315, 380
BfoI RGCGCY 1 cut(s) 571
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
BmcAI AGTACT 1 cut(s) 158
Bme1390I CCNGG 2 cut(s) 498, 666
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 498, 666
BpiI GAAGAC 3 cut(s) 287, 522, 735
BpmI CTGGAG 2 cut(s) 333, 480
BsaJI CCNNGG 1 cut(s) 664
BsaWI WCCGGW 2 cut(s) 40, 651
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 503
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 2 cut(s) 181, 630
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 2 cut(s) 498, 666
BseDI CCNNGG 1 cut(s) 664
BseGI GGATG 1 cut(s) 767
BseLI CCNNNNNNNGG 2 cut(s) 176, 503
BseMII CTCAG 2 cut(s) 64, 525
BseNI ACTGG 2 cut(s) 181, 630
BseRI GAGGAG 2 cut(s) 59, 479
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 3 cut(s) 51, 69, 249
BsiSI CCGG 3 cut(s) 9, 41, 652
BslFI GGGAC 2 cut(s) 209, 711
BslI CCNNNNNNNGG 2 cut(s) 176, 503
BsmFI GGGAC 2 cut(s) 209, 711
BsnI GGCC 1 cut(s) 169
Bsp1286I GDGCHC 3 cut(s) 51, 69, 249
Bsp13I TCCGGA 1 cut(s) 40
Bsp143I GATC 2 cut(s) 27, 88
BspACI CCGC 1 cut(s) 643
BspANI GGCC 1 cut(s) 169
BspCNI CTCAG 2 cut(s) 63, 524
BspEI TCCGGA 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 319
BspT107I GGYRCC 1 cut(s) 394
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 2 cut(s) 181, 630
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 2 cut(s) 27, 88
Bst2UI CCWGG 2 cut(s) 498, 666
Bst4CI ACNGT 2 cut(s) 293, 394
Bst6I CTCTTC 1 cut(s) 632
BstC8I GCNNGC 1 cut(s) 473
BstDEI CTNAG 4 cut(s) 50, 150, 476, 511
BstEII GGTNACC 1 cut(s) 659
BstF5I GGATG 1 cut(s) 767
BstH2I RGCGCY 1 cut(s) 571
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 2 cut(s) 30, 91
BstMBI GATC 2 cut(s) 27, 88
BstMWI GCNNNNNNNGC 2 cut(s) 73, 314
BstNI CCWGG 2 cut(s) 498, 666
BstNSI RCATGY 1 cut(s) 358
BstPI GGTNACC 1 cut(s) 659
BstSCI CCNGG 2 cut(s) 496, 664
BstSFI CTRYAG 2 cut(s) 315, 380
BstV1I GCAGC 1 cut(s) 218
BstV2I GAAGAC 3 cut(s) 287, 522, 735
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 169
BtsCI GGATG 1 cut(s) 767
BtsI GCAGTG 2 cut(s) 252, 365
BtsIMutI CAGTG 3 cut(s) 174, 252, 365
Cac8I GCNNGC 1 cut(s) 473
CaiI CAGNNNCTG 2 cut(s) 571, 625
CfoI GCGC 1 cut(s) 570
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 1 cut(s) 168
Csp6I GTAC 3 cut(s) 157, 164, 387
CspCI CAANNNNNGTGG 4 cut(s) 430, 465, 675, 710
CviAII CATG 4 cut(s) 119, 201, 355, 712
CviQI GTAC 3 cut(s) 157, 164, 387
DdeI CTNAG 4 cut(s) 50, 150, 476, 511
DpnI GATC 2 cut(s) 29, 90
DpnII GATC 2 cut(s) 27, 88
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
EciI GGCGGA 1 cut(s) 658
Ecl136II GAGCTC 3 cut(s) 49, 67, 247
Eco24I GRGCYC 3 cut(s) 51, 69, 249
Eco53kI GAGCTC 3 cut(s) 49, 67, 247
Eco57I CTGAAG 2 cut(s) 593, 656
Eco91I GGTNACC 1 cut(s) 659
EcoICRI GAGCTC 3 cut(s) 49, 67, 247
EcoO65I GGTNACC 1 cut(s) 659
EcoRII CCWGG 2 cut(s) 496, 664
EcoT38I GRGCYC 3 cut(s) 51, 69, 249
FaeI CATG 4 cut(s) 122, 204, 358, 715
FalI AAGNNNNNCTT 4 cut(s) 65, 97, 143, 175
FaqI GGGAC 2 cut(s) 209, 711
FatI CATG 4 cut(s) 118, 200, 354, 711
Fnu4HI GCNGC 1 cut(s) 232
FokI GGATG 1 cut(s) 754
FriOI GRGCYC 3 cut(s) 51, 69, 249
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 1 cut(s) 221
GlaI GCGC 1 cut(s) 569
GluI GCNGC 1 cut(s) 232
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 2 cut(s) 333, 480
HaeII RGCGCY 1 cut(s) 571
HaeIII GGCC 1 cut(s) 169
HapII CCGG 3 cut(s) 9, 41, 652
HhaI GCGC 1 cut(s) 570
Hin1II CATG 4 cut(s) 122, 204, 358, 715
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HindIII AAGCTT 3 cut(s) 79, 473, 759
HinfI GANTC 5 cut(s) 197, 587, 708, 730, 776
HpaII CCGG 3 cut(s) 9, 41, 652
HphI GGTGA 6 cut(s) 313, 430, 670, 671, 738, 774
Hpy188I TCNGA 4 cut(s) 53, 212, 409, 514
Hpy188III TCNNGA 4 cut(s) 41, 350, 539, 727
HpyAV CCTTC 3 cut(s) 447, 568, 685
HpyCH4III ACNGT 2 cut(s) 293, 394
HpyCH4V TGCA 6 cut(s) 234, 317, 358, 363, 471, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 314
HpyF3I CTNAG 4 cut(s) 50, 150, 476, 511
Hsp92II CATG 4 cut(s) 122, 204, 358, 715
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 1 cut(s) 40
Kzo9I GATC 2 cut(s) 27, 88
LmnI GCTCC 3 cut(s) 46, 252, 521
Lsp1109I GCAGC 1 cut(s) 218
MaeI CTAG 1 cut(s) 221
MaeIII GTNAC 2 cut(s) 319, 659
MalI GATC 2 cut(s) 29, 90
MboI GATC 2 cut(s) 27, 88
MboII GAAGA 7 cut(s) 29, 287, 527, 590, 649, 735, 771
MhlI GDGCHC 3 cut(s) 51, 69, 249
MluCI AATT 6 cut(s) 59, 286, 371, 403, 547, 687
MlyI GAGTC 2 cut(s) 191, 702
MnlI CCTC 8 cut(s) 37, 206, 335, 357, 408, 457, 633, 734
MroI TCCGGA 1 cut(s) 40
MseI TTAA 3 cut(s) 135, 491, 788
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 3 cut(s) 9, 41, 652
MspR9I CCNGG 2 cut(s) 498, 666
MvaI CCWGG 2 cut(s) 498, 666
MwoI GCNNNNNNNGC 2 cut(s) 73, 314
NdeII GATC 2 cut(s) 27, 88
NlaIII CATG 4 cut(s) 122, 204, 358, 715
NlaIV GGNNCC 1 cut(s) 396
NmuCI GTSAC 2 cut(s) 319, 659
NspI RCATGY 1 cut(s) 358
PfeI GAWTC 3 cut(s) 587, 730, 776
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 2 cut(s) 191, 702
PpsI GAGTC 2 cut(s) 191, 702
PshBI ATTAAT 1 cut(s) 788
PsiI TTATAA 1 cut(s) 285
Psp124BI GAGCTC 3 cut(s) 51, 69, 249
Psp6I CCWGG 2 cut(s) 496, 664
PspEI GGTNACC 1 cut(s) 659
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 2 cut(s) 496, 664
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 1 cut(s) 168
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
PstI CTGCAG 1 cut(s) 319
PstNI CAGNNNCTG 2 cut(s) 571, 625
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 3 cut(s) 158, 165, 388
RsaNI GTAC 3 cut(s) 157, 164, 387
SacI GAGCTC 3 cut(s) 51, 69, 249
SaqAI TTAA 3 cut(s) 135, 491, 788
SatI GCNGC 1 cut(s) 232
Sau3AI GATC 2 cut(s) 27, 88
Sau96I GGNCC 1 cut(s) 168
ScaI AGTACT 1 cut(s) 158
SchI GAGTC 2 cut(s) 191, 702
ScrFI CCNGG 2 cut(s) 498, 666
SduI GDGCHC 3 cut(s) 51, 69, 249
SfcI CTRYAG 2 cut(s) 315, 380
Sse9I AATT 6 cut(s) 59, 286, 371, 403, 547, 687
SsiI CCGC 1 cut(s) 643
SspMI CTAG 1 cut(s) 221
SstI GAGCTC 3 cut(s) 51, 69, 249
StyD4I CCNGG 2 cut(s) 496, 664
TaaI ACNGT 2 cut(s) 293, 394
TaqI TCGA 1 cut(s) 69
TasI AATT 6 cut(s) 59, 286, 371, 403, 547, 687
TatI WGTACW 2 cut(s) 156, 386
TfiI GAWTC 3 cut(s) 587, 730, 776
Tru1I TTAA 3 cut(s) 135, 491, 788
Tru9I TTAA 3 cut(s) 135, 491, 788
TscAI CASTG 3 cut(s) 181, 259, 365
TseFI GTSAC 2 cut(s) 319, 659
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 2 cut(s) 319, 659
TspDTI ATGAA 2 cut(s) 432, 753
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 3 cut(s) 181, 259, 365
VspI ATTAAT 1 cut(s) 788
XapI RAATTY 2 cut(s) 371, 403
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 221
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.