RchiOBHm_Chr7g0205251

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003766
Physical Location & Seq
Forward (+)
22915493 .. 22919728
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18367

Sequence Viewer

Length: 663 bp
ATGTGGTTTGGTGTGGTAATGTGCATATATGGCAGGTTGTGGCATGGTAGAGGATCGAAGAGAACCTTCTTATCTTGCTGTTCGTCTTTTAAGAGTGACGGTGGTGGTTTTCACTTGATCTCTGTTCATCCCGATGAGCTCAGATTTCCGTTTGAACTGAAAAAGGAAAGCTTTTGTGATCTAGAAGTTGTCAGTAATACAGAACACCATGTAGCTTTCAAGGTTAAAACCAGTGCACCTATGAAGTTCTTTGTATGGCCCAATATGGGGGTTATAAAGGCTCAGGACTCATGTATCATCAGAGTCAACCTCCGTGGCCAACGAAAATATCCTCCAAATATGGAGTGCAAAGATAAGTTTCTCCTACAGAGCACAATAGTGCCTCCAAATTCTGATATTGATGAGCTTCCAAAATATACCGAAGTTCCTGAAAACAAGCGTAGGTTGTCTATCTATAAAGGTATCGTTATTTCAAAGCAAAATGCTGGCATTCATACAACATTTCGCATTCGGAGGATAATTGCTGGCATCGGGGTTGAGATTGTTTTCCCTCTATACTCACCGAACATTAAGGAGTTGAAGGTACTTAGTCATAGGAAGGTCAGAAGGGCAAGGCTGTACTATTTGAGAGACAAACTTCCTAGGTTCTCGACTTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

220

Amino Acids

25.55

Weight (kDa)

9.93

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 40 - 132 1.4e-22 MSP (Major sperm protein) domain
Ribosomal_L19 PF01245 142 - 214 5.7e-25 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 24
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 1 cut(s) 388
AfaI GTAC 2 cut(s) 585, 620
AfiI CCNNNNNNNGG 2 cut(s) 266, 267
AgsI TTSAA 5 cut(s) 155, 220, 474, 580, 658
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 4 cut(s) 139, 171, 215, 406
AluI AGCT 4 cut(s) 139, 171, 215, 406
Alw21I GWGCWC 3 cut(s) 141, 238, 374
Alw26I GTCTC 1 cut(s) 624
Alw44I GTGCAC 1 cut(s) 234
AlwI GGATC 1 cut(s) 61
AoxI GGCC 2 cut(s) 257, 316
ApaLI GTGCAC 1 cut(s) 234
ApoI RAATTY 1 cut(s) 388
AspA2I CCTAGG 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 552
AvrII CCTAGG 1 cut(s) 641
BaeGI GKGCMC 1 cut(s) 238
BaeI ACNNNNGTAYC 2 cut(s) 277, 310
BalI TGGCCA 1 cut(s) 318
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 3 cut(s) 141, 238, 374
BcoDI GTCTC 1 cut(s) 624
BfaI CTAG 2 cut(s) 182, 642
BfmI CTRYAG 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 24
BlnI CCTAGG 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 258
BmsI GCATC 1 cut(s) 537
BplI GAGNNNNNCTC 2 cut(s) 294, 326
Bpu10I CCTNAGC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 313, 641
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 267
Bse1I ACTGG 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 313, 641
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 2 cut(s) 266, 267
BseMII CTCAG 2 cut(s) 154, 296
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 238
BshFI GGCC 2 cut(s) 259, 318
BsiHKAI GWGCWC 3 cut(s) 141, 238, 374
BslI CCNNNNNNNGG 2 cut(s) 266, 267
BsmAI GTCTC 1 cut(s) 624
BsmI GAATGC 2 cut(s) 489, 507
BsnI GGCC 2 cut(s) 259, 318
Bsp1286I GDGCHC 3 cut(s) 141, 238, 374
Bsp143I GATC 3 cut(s) 53, 117, 178
BspANI GGCC 2 cut(s) 259, 318
BspCNI CTCAG 2 cut(s) 153, 295
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 1 cut(s) 61
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 313, 641
BssMI GATC 3 cut(s) 53, 117, 178
BssT1I CCWWGG 1 cut(s) 641
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 487, 526
BstDEI CTNAG 3 cut(s) 140, 282, 587
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 3 cut(s) 56, 120, 181
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 3 cut(s) 53, 117, 178
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 365
BstSLI GKGCMC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 259, 318
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 487, 526
Cfr13I GGNCC 1 cut(s) 258
Csp6I GTAC 2 cut(s) 584, 619
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 3 cut(s) 44, 209, 291
CviJI RGCY 8 cut(s) 139, 171, 215, 259, 281, 318, 406, 616
CviKI_1 RGCY 8 cut(s) 139, 171, 215, 259, 281, 318, 406, 616
CviQI GTAC 2 cut(s) 584, 619
DdeI CTNAG 3 cut(s) 140, 282, 587
DpnI GATC 3 cut(s) 55, 119, 180
DpnII GATC 3 cut(s) 53, 117, 178
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 641
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 641
FaeI CATG 3 cut(s) 47, 212, 294
FalI AAGNNNNNCTT 4 cut(s) 50, 82, 155, 187
FatI CATG 3 cut(s) 43, 208, 290
FokI GGATG 1 cut(s) 114
FriOI GRGCYC 1 cut(s) 141
FspBI CTAG 2 cut(s) 182, 642
HaeIII GGCC 2 cut(s) 259, 318
Hin1II CATG 3 cut(s) 47, 212, 294
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 2 cut(s) 287, 303
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 2 cut(s) 236, 307
Hpy188I TCNGA 5 cut(s) 143, 302, 394, 513, 605
Hpy188III TCNNGA 5 cut(s) 131, 182, 284, 428, 649
Hpy8I GTNNAC 2 cut(s) 236, 307
HpyAV CCTTC 4 cut(s) 76, 574, 592, 600
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 24, 236, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 3 cut(s) 140, 282, 587
Hsp92II CATG 3 cut(s) 47, 212, 294
Kzo9I GATC 3 cut(s) 53, 117, 178
LpnPI CCDG 6 cut(s) 19, 244, 269, 441, 471, 510
LweI GCATC 1 cut(s) 537
MaeI CTAG 2 cut(s) 182, 642
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 55, 119, 180
MboI GATC 3 cut(s) 53, 117, 178
MboII GAAGA 1 cut(s) 70
MhlI GDGCHC 3 cut(s) 141, 238, 374
MlsI TGGCCA 1 cut(s) 318
MluCI AATT 2 cut(s) 388, 519
MluNI TGGCCA 1 cut(s) 318
MlyI GAGTC 2 cut(s) 281, 312
MnlI CCTC 6 cut(s) 44, 320, 342, 393, 507, 561
Mox20I TGGCCA 1 cut(s) 318
MscI TGGCCA 1 cut(s) 318
MseI TTAA 3 cut(s) 90, 225, 570
MslI CAYNNNNRTG 2 cut(s) 132, 377
Msp20I TGGCCA 1 cut(s) 318
Mva1269I GAATGC 2 cut(s) 489, 507
MwoI GCNNNNNNNGC 1 cut(s) 30
NdeII GATC 3 cut(s) 53, 117, 178
NlaIII CATG 3 cut(s) 47, 212, 294
NmuCI GTSAC 1 cut(s) 95
OliI CACNNNNGTG 1 cut(s) 377
PctI GAATGC 2 cut(s) 489, 507
PleI GAGTC 2 cut(s) 281, 311
PpsI GAGTC 2 cut(s) 281, 311
PsiI TTATAA 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 258
RsaI GTAC 2 cut(s) 585, 620
RsaNI GTAC 2 cut(s) 584, 619
RseI CAYNNNNRTG 2 cut(s) 132, 377
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 3 cut(s) 90, 225, 570
Sau3AI GATC 3 cut(s) 53, 117, 178
Sau96I GGNCC 1 cut(s) 258
SchI GAGTC 2 cut(s) 281, 312
SduI GDGCHC 3 cut(s) 141, 238, 374
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 132, 377
Sse9I AATT 2 cut(s) 388, 519
SspMI CTAG 2 cut(s) 182, 642
SstI GAGCTC 1 cut(s) 141
StyI CCWWGG 1 cut(s) 641
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 2 cut(s) 56, 650
TasI AATT 2 cut(s) 388, 519
TatI WGTACW 1 cut(s) 618
Tru1I TTAA 3 cut(s) 90, 225, 570
Tru9I TTAA 3 cut(s) 90, 225, 570
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 116, 257, 482
TspGWI ACGGA 2 cut(s) 138, 302
TspRI CASTG 1 cut(s) 238
VneI GTGCAC 1 cut(s) 234
XapI RAATTY 1 cut(s) 388
XbaI TCTAGA 1 cut(s) 181
XmaJI CCTAGG 1 cut(s) 641
XspI CTAG 2 cut(s) 182, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.