Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003766

Physical Location & Seq

Genomic Coordinates Reverse (-)

6549892 .. 6553733

Gene Structure 3842 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1048500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGAGTGCCGGTGGTGGTAAACAGTTGATTTCTGTTCTTCCTGATGAGCTCAAATTCCTATTTGAGCTGGAAAAGCAAAGCTTTTGTGATCTAAAAGTTGTCAATAACACAGAACACCATGTTGCTTTCAAGGTTAAAACTACTTCACCTAAGAAGTATTTTGTACGGCCCAACACTGGTGTTATACAGGCTTGGGATTCATGTGTAATCAGAGTCACCCTCCAAGCCCAACGAGAATATCCTCCAGATATGCAGTGCAAAGATAAATTTCTTCTACAAAGTACAACAGTGCCTCCAAATACTGATGTTGATGACCTTCCACAAGATACTTTCACTAAGGAAAGTGGAAGGGTTTTAGAGGAGTGCAAGCTTAGAGTTGTGTATGTCGCTTCGGGCGCTCAAGGAAACTCAGAAGATGAAGCTATGAGGAGCTCCATGCATAGTCTTGATACTAATTCTAATCAGGCTTTTCAGCGCCTGAAGGAAGAAAGAGATGCATCTGTTAGACAAACACAGCAACTTCAACAGGAACTGGATACACTGAAGAGGCGAAGATTTCGGAAGGGTGACCCAGGCTTTTCCTTCACCTTTGCAATTTTTGTGGGACTTATTGGACTCATGGTTGGCTTCCTTTTGAAACTTTCATTGTCCTCACCAACTACAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Motile_Sperm

Protein Analysis

223

Amino Acids

25.09

Weight (kDa)

7.65

Isoelectric Point (pI)

50.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cep192_D6	PF22076	10 - 77	8.1e-06	Cep192 domain 6
Motile_Sperm	PF00635	13 - 110	4.1e-28	MSP (Major sperm protein) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47180 AT5G47180
fragaria_vesca	FvH4_5g20180 FvH4_5g20180 FvH4_5g20180
malus_domestica	MD04G1051600.v1.1 MD06G1048500.v1.1
prunus_persica	Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1 Prupe.5G058700_v2.0.a1
pyrus_communis	pycom04g04530 pycom06g04160
rosa_chinensis	RchiOBHm_Chr7g0205251 RchiOBHm_Chr7g0205261 RchiOBHm_Chr7g0205271
rosa_laevigata	RLG00000003392
rosa_multiflora	Rmu_co8442685.1_g000001 Rmu_sc0013888.1_g000001
rosa_roxburghii	Rroxscaffold_3G00252520 Rroxscaffold_3G00252530
rosa_rugosa	Rorug07G0088800 Rorug07G0088900 Rorug07G0088900 Rorug07G0089000 Rorug07G0089100 Rorug07G0089200
rosa_samantha	Rh7AG219600 Rh7AG219700 Rh7CG232500 Rh7CG232600
rosa_wichuraiana	Rw7G018990 Rw7G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AcsI	RAATTY	2 cut(s)	53, 266
AcuI	CTGAAG	2 cut(s)	500, 563
AfaI	GTAC	2 cut(s)	165, 283
AfiI	CCNNNNNNNGG	1 cut(s)	176
AgsI	TTSAA	3 cut(s)	130, 524, 637
AjnI	CCWGG	1 cut(s)	571
AluBI	AGCT	6 cut(s)	49, 67, 81, 370, 422, 432
AluI	AGCT	6 cut(s)	49, 67, 81, 370, 422, 432
Alw21I	GWGCWC	2 cut(s)	51, 434
AlwNI	CAGNNNCTG	2 cut(s)	478, 532
AoxI	GGCC	1 cut(s)	167
ApoI	RAATTY	2 cut(s)	53, 266
AspLEI	GCGC	2 cut(s)	398, 477
AspS9I	GGNCC	1 cut(s)	168
AsuHPI	GGTGA	5 cut(s)	138, 208, 577, 578, 645
BanII	GRGCYC	2 cut(s)	51, 434
Bbv12I	GWGCWC	2 cut(s)	51, 434
BceAI	ACGGC	1 cut(s)	182
BciT130I	CCWGG	1 cut(s)	573
BciVI	GTATCC	1 cut(s)	529
BfmI	CTRYAG	1 cut(s)	660
BfoI	RGCGCY	2 cut(s)	399, 478
BfuI	GTATCC	1 cut(s)	529
Bme1390I	CCNGG	1 cut(s)	573
BmgT120I	GGNCC	1 cut(s)	168
BmrFI	CCNGG	1 cut(s)	573
BmsI	GCATC	2 cut(s)	484, 506
BplI	GAGNNNNNCTC	2 cut(s)	204, 236
BpmI	CTGGAG	1 cut(s)	228
BpuEI	CTTGAG	1 cut(s)	384
BsaJI	CCNNGG	1 cut(s)	571
BsaXI	ACNNNNNCTCC	2 cut(s)	277, 307
Bsc4I	CCNNNNNNNGG	1 cut(s)	176
Bse118I	RCCGGY	1 cut(s)	8
Bse1I	ACTGG	2 cut(s)	181, 537
BseBI	CCWGG	1 cut(s)	573
BseDI	CCNNGG	1 cut(s)	571
BseLI	CCNNNNNNNGG	1 cut(s)	176
BseMII	CTCAG	1 cut(s)	423
BseNI	ACTGG	2 cut(s)	181, 537
BseRI	GAGGAG	2 cut(s)	374, 442
BshFI	GGCC	1 cut(s)	169
BsiHKAI	GWGCWC	2 cut(s)	51, 434
BsiSI	CCGG	1 cut(s)	9
BslFI	GGGAC	1 cut(s)	618
BslI	CCNNNNNNNGG	1 cut(s)	176
BsmFI	GGGAC	1 cut(s)	618
BsnI	GGCC	1 cut(s)	169
Bsp1286I	GDGCHC	2 cut(s)	51, 434
Bsp143I	GATC	1 cut(s)	88
BspANI	GGCC	1 cut(s)	169
BspCNI	CTCAG	1 cut(s)	422
BsrFI	RCCGGY	1 cut(s)	8
BsrI	ACTGG	2 cut(s)	181, 537
BssAI	RCCGGY	1 cut(s)	8
BssECI	CCNNGG	1 cut(s)	571
BssMI	GATC	1 cut(s)	88
Bst2UI	CCWGG	1 cut(s)	573
Bst4CI	ACNGT	2 cut(s)	24, 289
Bst6I	CTCTTC	1 cut(s)	539
BstC8I	GCNNGC	1 cut(s)	368
BstDEI	CTNAG	4 cut(s)	150, 336, 371, 409
BstEII	GGTNACC	1 cut(s)	566
BstH2I	RGCGCY	2 cut(s)	399, 478
BstHHI	GCGC	2 cut(s)	398, 477
BstKTI	GATC	1 cut(s)	91
BstMBI	GATC	1 cut(s)	88
BstMWI	GCNNNNNNNGC	2 cut(s)	73, 395
BstNI	CCWGG	1 cut(s)	573
BstPI	GGTNACC	1 cut(s)	566
BstSCI	CCNGG	1 cut(s)	571
BstSFI	CTRYAG	1 cut(s)	660
BsuI	GTATCC	1 cut(s)	529
BsuRI	GGCC	1 cut(s)	169
BtsI	GCAGTG	1 cut(s)	260
BtsIMutI	CAGTG	4 cut(s)	174, 260, 294, 539
Cac8I	GCNNGC	1 cut(s)	368
CaiI	CAGNNNCTG	2 cut(s)	478, 532
CfoI	GCGC	2 cut(s)	398, 477
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	1 cut(s)	168
Csp6I	GTAC	2 cut(s)	164, 282
CspCI	CAANNNNNGTGG	2 cut(s)	582, 617
CviAII	CATG	4 cut(s)	119, 201, 436, 619
CviQI	GTAC	2 cut(s)	164, 282
DdeI	CTNAG	4 cut(s)	150, 336, 371, 409
DpnI	GATC	1 cut(s)	90
DpnII	GATC	1 cut(s)	88
Eam1104I	CTCTTC	1 cut(s)	539
EarI	CTCTTC	1 cut(s)	539
Ecl136II	GAGCTC	2 cut(s)	49, 432
Eco24I	GRGCYC	2 cut(s)	51, 434
Eco53kI	GAGCTC	2 cut(s)	49, 432
Eco57I	CTGAAG	2 cut(s)	500, 563
Eco91I	GGTNACC	1 cut(s)	566
EcoICRI	GAGCTC	2 cut(s)	49, 432
EcoO65I	GGTNACC	1 cut(s)	566
EcoRII	CCWGG	1 cut(s)	571
EcoT22I	ATGCAT	2 cut(s)	441, 499
EcoT38I	GRGCYC	2 cut(s)	51, 434
FaeI	CATG	4 cut(s)	122, 204, 439, 622
FaiI	YATR	9 cut(s)	120, 185, 202, 251, 384, 425, 437, 441, 620
FalI	AAGNNNNNCTT	2 cut(s)	65, 97
FaqI	GGGAC	1 cut(s)	618
FatI	CATG	4 cut(s)	118, 200, 435, 618
FriOI	GRGCYC	2 cut(s)	51, 434
GlaI	GCGC	2 cut(s)	397, 476
GsuI	CTGGAG	1 cut(s)	228
HaeII	RGCGCY	2 cut(s)	399, 478
HaeIII	GGCC	1 cut(s)	169
HapII	CCGG	1 cut(s)	9
HhaI	GCGC	2 cut(s)	398, 477
Hin1II	CATG	4 cut(s)	122, 204, 439, 622
Hin6I	GCGC	2 cut(s)	396, 475
HinP1I	GCGC	2 cut(s)	396, 475
HindIII	AAGCTT	2 cut(s)	79, 368
HinfI	GANTC	3 cut(s)	197, 213, 615
HpaII	CCGG	1 cut(s)	9
HphI	GGTGA	5 cut(s)	138, 208, 577, 578, 645
Hpy166II	GTNNAC	1 cut(s)	20
Hpy188I	TCNGA	3 cut(s)	212, 412, 561
Hpy188III	TCNNGA	3 cut(s)	41, 245, 446
Hpy8I	GTNNAC	1 cut(s)	20
HpyAV	CCTTC	5 cut(s)	326, 342, 475, 556, 592
HpyCH4III	ACNGT	2 cut(s)	24, 289
HpyCH4V	TGCA	6 cut(s)	253, 258, 366, 439, 497, 593
HpyF10VI	GCNNNNNNNGC	2 cut(s)	73, 395
HpyF3I	CTNAG	4 cut(s)	150, 336, 371, 409
Hsp92II	CATG	4 cut(s)	122, 204, 439, 622
HspAI	GCGC	2 cut(s)	396, 475
Kzo9I	GATC	1 cut(s)	88
LmnI	GCTCC	2 cut(s)	429, 437
LweI	GCATC	2 cut(s)	484, 506
MaeIII	GTNAC	2 cut(s)	214, 566
MalI	GATC	1 cut(s)	90
MboI	GATC	1 cut(s)	88
MboII	GAAGA	6 cut(s)	29, 263, 425, 497, 556, 564
MhlI	GDGCHC	2 cut(s)	51, 434
MluCI	AATT	4 cut(s)	53, 266, 454, 594
MlyI	GAGTC	2 cut(s)	222, 609
MnlI	CCTC	7 cut(s)	230, 252, 303, 352, 420, 540, 661
Mph1103I	ATGCAT	2 cut(s)	441, 499
MseI	TTAA	1 cut(s)	135
MspI	CCGG	1 cut(s)	9
MspR9I	CCNGG	1 cut(s)	573
MvaI	CCWGG	1 cut(s)	573
MwoI	GCNNNNNNNGC	2 cut(s)	73, 395
NdeII	GATC	1 cut(s)	88
NlaIII	CATG	4 cut(s)	122, 204, 439, 622
NmuCI	GTSAC	2 cut(s)	214, 566
NsiI	ATGCAT	2 cut(s)	441, 499
PfeI	GAWTC	1 cut(s)	197
PleI	GAGTC	2 cut(s)	221, 609
PpsI	GAGTC	2 cut(s)	221, 609
Psp124BI	GAGCTC	2 cut(s)	51, 434
Psp6I	CCWGG	1 cut(s)	571
PspEI	GGTNACC	1 cut(s)	566
PspGI	CCWGG	1 cut(s)	571
PspPI	GGNCC	1 cut(s)	168
PstNI	CAGNNNCTG	2 cut(s)	478, 532
RsaI	GTAC	2 cut(s)	165, 283
RsaNI	GTAC	2 cut(s)	164, 282
SacI	GAGCTC	2 cut(s)	51, 434
SaqAI	TTAA	1 cut(s)	135
Sau3AI	GATC	1 cut(s)	88
Sau96I	GGNCC	1 cut(s)	168
SchI	GAGTC	2 cut(s)	222, 609
ScrFI	CCNGG	1 cut(s)	573
SduI	GDGCHC	2 cut(s)	51, 434
SfaNI	GCATC	2 cut(s)	484, 506
SfcI	CTRYAG	1 cut(s)	660
SmlI	CTYRAG	1 cut(s)	399
SmoI	CTYRAG	1 cut(s)	399
Sse9I	AATT	4 cut(s)	53, 266, 454, 594
SstI	GAGCTC	2 cut(s)	51, 434
StyD4I	CCNGG	1 cut(s)	571
TaaI	ACNGT	2 cut(s)	24, 289
TasI	AATT	4 cut(s)	53, 266, 454, 594
TatI	WGTACW	1 cut(s)	281
TfiI	GAWTC	1 cut(s)	197
Tru1I	TTAA	1 cut(s)	135
Tru9I	TTAA	1 cut(s)	135
TscAI	CASTG	4 cut(s)	181, 260, 294, 546
TseFI	GTSAC	2 cut(s)	214, 566
Tsp45I	GTSAC	2 cut(s)	214, 566
TspDTI	ATGAA	3 cut(s)	189, 432, 633
TspRI	CASTG	4 cut(s)	181, 260, 294, 546
XapI	RAATTY	2 cut(s)	53, 266
Zsp2I	ATGCAT	2 cut(s)	441, 499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.