FvH4_5g26300

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002954
Physical Location & Seq
Forward (+)
17802198 .. 17805528
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26300.t1

Sequence Viewer

Length: 1305 bp
ATGTCGGTGACGGCAGGTGTTAGTGATACTGTGATTGCCATTAGGGATAAGCTGAGAGGTAAAATTGGACAAACTAAGGTTAAGAGGTATTGGCCCGGAAAAGCTCCCGAGTGGGGAGATGATGCGAATGAAGATGGCGATATTAGGATGTCTGCGGCTGCCTCCCTCGAGAAAGCCTTCCCCACTCAGGATTATTCGGATGTCGGAAGGAAAGATGATCCGAGGCTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGTGAGGATGTTAGGGCTGATCATAGGCGTATCCGGCAAGCTGAGATTGTTTCGACGATTGAAGAGGAAGCCAGGAGGCAGGAAGGCATAGATGCGGAGGAAGAGGATCCGGATGCTTTAGAGGAAAAGAGGAGAAGGATTAAGGAGAGGTTGCGTCAGAGGGAGCAAGAAGAAGCTCCACTCCTGCCCTCGGAAGATGAGGAAGAAGATAAGGAAGAGGAGGAGGAAGAGGAATCAGAGTATTCAACTGACTCTGAAGATGAGCATGGTGGTATGATGATGCTGAAGCCAGTTTTTGTTCCCAAGGCTGAGAGAGATACCATCGCTGAACGGGAGCGAATTGAGGCAGAAGAACTGGCCCTTGAGGAAGCAAGGAAGAAAAAATTGGAGGAGAGGAAAAGAGAGACAAAGCAGATTGTGGTTGAGGAAATCCGAAAGGATGAAGAGATTCAGAAAGGTATGGAACAGGACGCTAACATTGCTGATATTGATACTGATGATGAACTTAACGAGGCCGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCAGGATCAAGAGGGATAGGGAGGAAAGGGAAGCATTGTTGAAGGAAAAGGAAGAGATTGAAAGAGTAAGAAATATGACAGAAGAAGAGAGGAGGGAATGGGAGAGGAAGAATCCTAAACCTGCACCACGACCAAAGCAAAAGTGGAGGTTCATGCAGAAATACTACCACAAAGGCGCTTTCTTCCAGTCGGACGCAGATGACCATGCTGCAACTGTTGGAACAGATGGAATTTTCACTCGTGATTTCTCTGCTCCAACTGGAGAAGATAAAATGGACAAAACTATATTGCCGAAGGTCATGCAAGTCAAGCATTTTGGTCGTAGTGGGAGGACAAAATGGACCCATCTTGTGAATGAGGATACTACTGACTGGAACAATCCGTGGACCTACAATGATCCTCTTCGGGGAAAGTACAATGCTAAAATGGCAGGAATGAATGCCCCTATAGAGAAACCCAGAGGAAGCAAGAAGTTGAAGGATCACAAGTATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

435

Amino Acids

51.06

Weight (kDa)

5.11

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 170 - 391 2.8e-77 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
Acc36I ACCTGC 2 cut(s) 5, 937
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 2 cut(s) 155, 353
AclWI GGATC 6 cut(s) 212, 359, 372, 821, 1199, 1296
AcsI RAATTY 1 cut(s) 1038
AcuI CTGAAG 2 cut(s) 534, 563
AfaI GTAC 1 cut(s) 1223
AfiI CCNNNNNNNGG 4 cut(s) 113, 187, 448, 1214
AgsI TTSAA 5 cut(s) 320, 504, 850, 869, 1285
AjnI CCWGG 2 cut(s) 329, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AluBI AGCT 5 cut(s) 52, 104, 299, 434, 788
AluI AGCT 5 cut(s) 52, 104, 299, 434, 788
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 6 cut(s) 212, 359, 372, 821, 1199, 1296
Ama87I CYCGRG 2 cut(s) 107, 167
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 3 cut(s) 92, 615, 771
ApeKI GCWGC 3 cut(s) 158, 226, 1016
ApoI RAATTY 1 cut(s) 1038
Asp700I GAANNNNTTC 2 cut(s) 176, 705
AspLEI GCGC 1 cut(s) 986
AspS9I GGNCC 4 cut(s) 93, 616, 1149, 1194
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 2 cut(s) 107, 167
AvaII GGWCC 2 cut(s) 1149, 1194
BamHI GGATCC 1 cut(s) 364
BauI CACGAG 1 cut(s) 1047
BbvI GCAGC 3 cut(s) 145, 213, 1003
BccI CCATC 4 cut(s) 128, 587, 1028, 1161
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 4 cut(s) 1090, 1109, 1124, 1143
BciT130I CCWGG 2 cut(s) 331, 811
BciVI GTATCC 2 cut(s) 299, 1162
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 656
BfmI CTRYAG 1 cut(s) 1254
BfoI RGCGCY 1 cut(s) 987
BfuAI ACCTGC 2 cut(s) 5, 937
BfuI GTATCC 2 cut(s) 299, 1162
BisI GCNGC 4 cut(s) 156, 159, 227, 1017
BlsI GCNGC 4 cut(s) 157, 160, 228, 1018
Bme1390I CCNGG 3 cut(s) 96, 331, 811
Bme18I GGWCC 2 cut(s) 1149, 1194
BmeT110I CYCGRG 2 cut(s) 107, 167
BmgT120I GGNCC 4 cut(s) 93, 616, 1149, 1194
BmiI GGNNCC 2 cut(s) 366, 1151
BmrFI CCNGG 3 cut(s) 96, 331, 811
BmsI GCATC 4 cut(s) 112, 340, 361, 528
BpmI CTGGAG 1 cut(s) 1089
BpuEI CTTGAG 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 5 cut(s) 221, 447, 561, 774, 1190
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 4 cut(s) 113, 187, 448, 1214
Bse1I ACTGG 5 cut(s) 548, 618, 994, 1072, 1184
Bse3DI GCAATG 1 cut(s) 735
BseAI TCCGGA 1 cut(s) 367
BseBI CCWGG 2 cut(s) 331, 811
BseDI CCNNGG 5 cut(s) 221, 447, 561, 774, 1190
BseGI GGATG 5 cut(s) 153, 205, 271, 376, 703
BseLI CCNNNNNNNGG 4 cut(s) 113, 187, 448, 1214
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 5 cut(s) 44, 200, 231, 291, 558
BseNI ACTGG 5 cut(s) 548, 618, 994, 1072, 1184
BseRI GAGGAG 6 cut(s) 403, 491, 494, 662, 791, 913
BseXI GCAGC 3 cut(s) 145, 213, 1003
BsgI GTGCAG 1 cut(s) 915
BshFI GGCC 3 cut(s) 94, 617, 773
BsiHKCI CYCGRG 2 cut(s) 107, 167
BsiSI CCGG 3 cut(s) 96, 292, 368
BslI CCNNNNNNNGG 4 cut(s) 113, 187, 448, 1214
BsmAI GTCTC 1 cut(s) 656
BsmI GAATGC 1 cut(s) 1252
BsnI GGCC 3 cut(s) 94, 617, 773
BsoBI CYCGRG 2 cut(s) 107, 167
Bsp13I TCCGGA 1 cut(s) 367
Bsp143I GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
BspACI CCGC 2 cut(s) 155, 353
BspANI GGCC 3 cut(s) 94, 617, 773
BspCNI CTCAG 5 cut(s) 45, 199, 232, 292, 559
BspEI TCCGGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 366, 1151
BspMI ACCTGC 2 cut(s) 5, 937
BspPI GGATC 6 cut(s) 212, 359, 372, 821, 1199, 1296
BsrDI GCAATG 1 cut(s) 735
BsrI ACTGG 5 cut(s) 548, 618, 994, 1072, 1184
BssECI CCNNGG 5 cut(s) 221, 447, 561, 774, 1190
BssMI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
BssSI CACGAG 1 cut(s) 1047
BssT1I CCWWGG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 1047
Bst2UI CCWGG 2 cut(s) 331, 811
Bst4CI ACNGT 2 cut(s) 31, 1024
Bst6I CTCTTC 8 cut(s) 315, 354, 468, 480, 696, 855, 888, 1215
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 6 cut(s) 53, 75, 186, 240, 300, 567
BstDSI CCRYGG 1 cut(s) 1190
BstF5I GGATG 5 cut(s) 153, 205, 271, 376, 703
BstH2I RGCGCY 1 cut(s) 987
BstHHI GCGC 1 cut(s) 986
BstKTI GATC 6 cut(s) 220, 280, 367, 816, 1207, 1291
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
BstMWI GCNNNNNNNGC 5 cut(s) 292, 737, 794, 1117, 1235
BstNI CCWGG 2 cut(s) 331, 811
BstSCI CCNGG 3 cut(s) 94, 329, 809
BstSFI CTRYAG 1 cut(s) 1254
BstV1I GCAGC 3 cut(s) 145, 213, 1003
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 2 cut(s) 299, 1162
BsuRI GGCC 3 cut(s) 94, 617, 773
BtgI CCRYGG 1 cut(s) 1190
BtgZI GCGATG 1 cut(s) 565
BtsCI GGATG 5 cut(s) 153, 205, 271, 376, 703
BveI ACCTGC 2 cut(s) 5, 937
Cac8I GCNNGC 1 cut(s) 297
CfoI GCGC 1 cut(s) 986
Cfr13I GGNCC 4 cut(s) 93, 616, 1149, 1194
CseI GACGC 4 cut(s) 218, 401, 737, 1010
Csp6I GTAC 1 cut(s) 1222
CviAII CATG 4 cut(s) 524, 961, 1013, 1108
CviQI GTAC 1 cut(s) 1222
DdeI CTNAG 6 cut(s) 53, 75, 186, 240, 300, 567
DpnI GATC 6 cut(s) 219, 279, 366, 815, 1206, 1290
DpnII GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
Eam1104I CTCTTC 8 cut(s) 315, 354, 468, 480, 696, 855, 888, 1215
EarI CTCTTC 8 cut(s) 315, 354, 468, 480, 696, 855, 888, 1215
Eco130I CCWWGG 1 cut(s) 561
Eco47I GGWCC 2 cut(s) 1149, 1194
Eco57I CTGAAG 2 cut(s) 534, 563
Eco88I CYCGRG 2 cut(s) 107, 167
EcoRII CCWGG 2 cut(s) 329, 809
EcoT14I CCWWGG 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 4 cut(s) 527, 964, 1016, 1111
FatI CATG 4 cut(s) 523, 960, 1012, 1107
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 156, 159, 227, 1017
FokI GGATG 5 cut(s) 160, 212, 278, 383, 710
Fsp4HI GCNGC 4 cut(s) 156, 159, 227, 1017
GlaI GCGC 1 cut(s) 985
GluI GCNGC 4 cut(s) 156, 159, 227, 1017
GsuI CTGGAG 1 cut(s) 1089
HaeII RGCGCY 1 cut(s) 987
HaeIII GGCC 3 cut(s) 94, 617, 773
HapII CCGG 3 cut(s) 96, 292, 368
HgaI GACGC 4 cut(s) 218, 401, 737, 1010
HhaI GCGC 1 cut(s) 986
Hin1II CATG 4 cut(s) 527, 964, 1016, 1111
Hin6I GCGC 1 cut(s) 984
HinP1I GCGC 1 cut(s) 984
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 4 cut(s) 491, 509, 706, 919
HpaII CCGG 3 cut(s) 96, 292, 368
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 1194
Hpy188III TCNNGA 7 cut(s) 107, 169, 188, 260, 368, 817, 1049
Hpy8I GTNNAC 1 cut(s) 1194
Hpy99I CGWCG 2 cut(s) 234, 316
HpyAV CCTTC 8 cut(s) 187, 201, 335, 387, 787, 844, 1096, 1279
HpyCH4III ACNGT 2 cut(s) 31, 1024
HpyCH4V TGCA 4 cut(s) 932, 964, 1019, 1111
HpyF10VI GCNNNNNNNGC 5 cut(s) 292, 737, 794, 1117, 1235
HpyF3I CTNAG 6 cut(s) 53, 75, 186, 240, 300, 567
Hsp92II CATG 4 cut(s) 527, 964, 1016, 1111
HspAI GCGC 1 cut(s) 984
Kpn2I TCCGGA 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
LmnI GCTCC 5 cut(s) 109, 421, 439, 592, 1066
Lsp1109I GCAGC 3 cut(s) 145, 213, 1003
LweI GCATC 4 cut(s) 112, 340, 361, 528
MaeIII GTNAC 1 cut(s) 7
MalI GATC 6 cut(s) 219, 279, 366, 815, 1206, 1290
MboI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
MflI RGATCY 1 cut(s) 364
MluCI AATT 4 cut(s) 63, 597, 641, 1038
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 4 cut(s) 184, 978, 1006, 1088
MroI TCCGGA 1 cut(s) 367
MroXI GAANNNNTTC 2 cut(s) 176, 705
MseI TTAA 3 cut(s) 81, 399, 765
MspI CCGG 3 cut(s) 96, 292, 368
MspR9I CCNGG 3 cut(s) 96, 331, 811
Mva1269I GAATGC 1 cut(s) 1252
MvaI CCWGG 2 cut(s) 331, 811
MwoI GCNNNNNNNGC 5 cut(s) 292, 737, 794, 1117, 1235
NciI CCSGG 1 cut(s) 96
NdeII GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
NlaIII CATG 4 cut(s) 527, 964, 1016, 1111
NlaIV GGNNCC 2 cut(s) 366, 1151
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 1 cut(s) 7
PaeR7I CTCGAG 1 cut(s) 167
PaqCI CACCTGC 1 cut(s) 5
PctI GAATGC 1 cut(s) 1252
PdmI GAANNNNTTC 2 cut(s) 176, 705
PfeI GAWTC 3 cut(s) 491, 706, 919
PkrI GCNGC 4 cut(s) 157, 160, 228, 1018
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 329, 809
PspGI CCWGG 2 cut(s) 329, 809
PspN4I GGNNCC 2 cut(s) 366, 1151
PspPI GGNCC 4 cut(s) 93, 616, 1149, 1194
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 1 cut(s) 1223
RsaNI GTAC 1 cut(s) 1222
SaqAI TTAA 3 cut(s) 81, 399, 765
SatI GCNGC 4 cut(s) 156, 159, 227, 1017
Sau3AI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
Sau96I GGNCC 4 cut(s) 93, 616, 1149, 1194
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 96, 331, 811
SfaNI GCATC 4 cut(s) 112, 340, 361, 528
SfcI CTRYAG 1 cut(s) 1254
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 2 cut(s) 1149, 1194
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 167, 620
SmoI CTYRAG 2 cut(s) 167, 620
Sse9I AATT 4 cut(s) 63, 597, 641, 1038
SsiI CCGC 2 cut(s) 155, 353
StyD4I CCNGG 3 cut(s) 94, 329, 809
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 31, 1024
TaqI TCGA 2 cut(s) 168, 311
TasI AATT 4 cut(s) 63, 597, 641, 1038
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 3 cut(s) 491, 706, 919
Tru1I TTAA 3 cut(s) 81, 399, 765
Tru9I TTAA 3 cut(s) 81, 399, 765
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 3 cut(s) 158, 226, 1016
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 6 cut(s) 144, 714, 774, 798, 949, 1259
TspGWI ACGGA 1 cut(s) 1179
VpaK11BI GGWCC 2 cut(s) 1149, 1194
XapI RAATTY 1 cut(s) 1038
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XhoI CTCGAG 1 cut(s) 167
XmnI GAANNNNTTC 2 cut(s) 176, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.