RLG00000018476

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002954
Physical Location & Seq
Reverse (-)
30480654 .. 30482344
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018476

Sequence Viewer

Length: 1305 bp
ATGTCGGTGACAGCAGGTGTTAGTGATACTGTGATTGCGATTAGGGATAAGCTGAGAGGTAAAATTGGACAAACTAAAGTTAAAAGGTATTGGCCCGGAAAAGCTCCCGAGTGGGGAGATGATGCGGATGAAGATGGCGATATTAGGATGTCCGCGGCTGCCTCCCTGGAGAAAGCCTTTCCCACTCAGGATTATTCGGATGTTGGAAGGAAAGATGATCCTAGGCTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGCGAGGATGTTAGGGCTGATCATAGGCGTATCCGGCAAGCTGAAATTGTTTCAACAATTGAAGAGGAAGCCAAGAGGCAGGAAGGGATAGATGCAGAAGAAGAGGATCCTGATGCTTTAGAGGAAAGGAGGAGAAGGATTAAGGAAAGGTTGCGTCAGAGGGAGCAAGAAGAAACTCCACTCCTCCCCTCGGAAGATGAGGAAGAAGATAAGGAAGAGGAGGAGGAAGAGGAATCAGAGTATTCAACTGACTCAGAAGATGAGCATGGTGGCATGATGATGCTGAAGCCAGTCTTTGTTCCGAAGGCTGAGAGAGATACAATTGCTGAGCGTGAGCGAATTGAGGCAGAAGAGTTGGCCCTCGAGGAAGCAAGGAAGAAAAAATTGGAGGAGAGGAAGAGAGAGACGAAGCAGATTGTGGTTGAGGAAATCCGAAAGGATGAAGAGATTCAGAAGGGTATGGAACAGGACGCTAACATTGCTGATATTGATACTGATGATGAACTTAATGAGGCAGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCAGGATCAAGAGGGATAGAGAGGAGAGGGAAGCTATGTTGAAGGAAAAGGAAGAGATAGAAAGAGTAAGAAATATGACAGAGGAAGAGAGGAGGGAATGGGAGAGGAAGAATCCTAAACCTACACCTCGACCAAAGCAAAAGTGGAGGTTCATGCAGAAATATTACCACAAAGGTGCTTTCTTCCAGTCAGATGCAGATGACCATGCTGCAACTGTTGGAACAGATGGAATTTTCACTCGTGATTTCTCTGCTCCAACTGGAGAAGATAAGATGGATAAAACTATATTGCCGAAGGTGATGCAAGTCAAGCATTTTGGTCGTAGTGGAAGGACAAAATGGACCCATCTTGTCAATGAGGATACTACTGACTGGAACAACCCGTGGACGTACAATGATCCTCTTCGGGCAAAGTATAATGCTAAAATGGCCGGAATGAATGCACCTATAGAAAAACCCAGAGGAAGCAAGAAATTGAAGGATCAGAAGTATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

435

Amino Acids

51.15

Weight (kDa)

5.05

Isoelectric Point (pI)

59.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 170 - 391 2.3e-77 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
AbsI CCTCGAGG 1 cut(s) 620
Acc36I ACCTGC 1 cut(s) 5
AccII CGCG 2 cut(s) 155, 261
AciI CCGC 3 cut(s) 125, 153, 155
AclWI GGATC 6 cut(s) 212, 359, 372, 821, 1199, 1296
AcoI YGGCCR 1 cut(s) 1236
AcsI RAATTY 1 cut(s) 1038
AcuI CTGAAG 1 cut(s) 563
AfaI GTAC 1 cut(s) 1199
AfiI CCNNNNNNNGG 2 cut(s) 113, 448
AgsI TTSAA 5 cut(s) 312, 320, 504, 850, 1285
AjnI CCWGG 2 cut(s) 165, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AleI CACNNNNGTG 1 cut(s) 981
AluBI AGCT 5 cut(s) 52, 104, 299, 788, 842
AluI AGCT 5 cut(s) 52, 104, 299, 788, 842
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 6 cut(s) 212, 359, 372, 821, 1199, 1296
Ama87I CYCGRG 2 cut(s) 107, 620
AoxI GGCC 3 cut(s) 92, 615, 1236
ApeKI GCWGC 3 cut(s) 158, 226, 1016
ApoI RAATTY 1 cut(s) 1038
Asp700I GAANNNNTTC 1 cut(s) 705
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 93, 616, 1149
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 19, 1117
AvaI CYCGRG 2 cut(s) 107, 620
AvaII GGWCC 1 cut(s) 1149
AvrII CCTAGG 1 cut(s) 221
BamHI GGATCC 1 cut(s) 364
BauI CACGAG 1 cut(s) 1047
BbvI GCAGC 3 cut(s) 145, 213, 1003
BccI CCATC 4 cut(s) 128, 1028, 1075, 1161
BcgI CGANNNNNNTGC 4 cut(s) 1090, 1109, 1124, 1143
BciT130I CCWGG 2 cut(s) 167, 811
BciVI GTATCC 2 cut(s) 299, 1162
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 1254
BfuAI ACCTGC 1 cut(s) 5
BfuI GTATCC 2 cut(s) 299, 1162
BisI GCNGC 4 cut(s) 156, 159, 227, 1017
BlnI CCTAGG 1 cut(s) 221
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 4 cut(s) 157, 160, 228, 1018
Bme1390I CCNGG 3 cut(s) 96, 167, 811
Bme18I GGWCC 1 cut(s) 1149
BmeT110I CYCGRG 2 cut(s) 107, 620
BmgT120I GGNCC 3 cut(s) 93, 616, 1149
BmiI GGNNCC 2 cut(s) 366, 1151
BmrFI CCNGG 3 cut(s) 96, 167, 811
BmsI GCATC 6 cut(s) 112, 340, 361, 528, 991, 1098
BpmI CTGGAG 2 cut(s) 188, 1089
Bpu1102I GCTNAGC 1 cut(s) 585
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 5 cut(s) 153, 165, 221, 447, 1190
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 448
Bse1I ACTGG 4 cut(s) 548, 994, 1072, 1184
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 167, 811
BseDI CCNNGG 5 cut(s) 153, 165, 221, 447, 1190
BseGI GGATG 5 cut(s) 133, 153, 205, 271, 703
BseLI CCNNNNNNNGG 2 cut(s) 113, 448
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 6 cut(s) 44, 200, 231, 525, 558, 576
BseNI ACTGG 4 cut(s) 548, 994, 1072, 1184
BseRI GAGGAG 8 cut(s) 403, 431, 491, 494, 662, 791, 845, 913
BseXI GCAGC 3 cut(s) 145, 213, 1003
Bsh1236I CGCG 2 cut(s) 155, 261
BshFI GGCC 3 cut(s) 94, 617, 1238
BsiHKCI CYCGRG 2 cut(s) 107, 620
BsiSI CCGG 3 cut(s) 96, 292, 1239
BslI CCNNNNNNNGG 2 cut(s) 113, 448
BsmAI GTCTC 1 cut(s) 656
BsmBI CGTCTC 1 cut(s) 656
BsmI GAATGC 1 cut(s) 1252
BsnI GGCC 3 cut(s) 94, 617, 1238
BsoBI CYCGRG 2 cut(s) 107, 620
Bsp143I GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
Bsp1720I GCTNAGC 1 cut(s) 585
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 3 cut(s) 125, 153, 155
BspANI GGCC 3 cut(s) 94, 617, 1238
BspCNI CTCAG 6 cut(s) 45, 199, 232, 524, 559, 577
BspFNI CGCG 2 cut(s) 155, 261
BspLI GGNNCC 2 cut(s) 366, 1151
BspMI ACCTGC 1 cut(s) 5
BspPI GGATC 6 cut(s) 212, 359, 372, 821, 1199, 1296
BsrDI GCAATG 1 cut(s) 735
BsrI ACTGG 4 cut(s) 548, 994, 1072, 1184
BssECI CCNNGG 5 cut(s) 153, 165, 221, 447, 1190
BssMI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
BssSI CACGAG 1 cut(s) 1047
BssT1I CCWWGG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 1047
Bst2UI CCWGG 2 cut(s) 167, 811
Bst4CI ACNGT 2 cut(s) 31, 1024
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 6 cut(s) 53, 186, 240, 511, 567, 585
BstDSI CCRYGG 2 cut(s) 153, 1190
BstF5I GGATG 5 cut(s) 133, 153, 205, 271, 703
BstFNI CGCG 2 cut(s) 155, 261
BstKTI GATC 6 cut(s) 220, 280, 367, 816, 1207, 1291
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
BstMWI GCNNNNNNNGC 5 cut(s) 292, 737, 794, 1117, 1235
BstNI CCWGG 2 cut(s) 167, 811
BstSCI CCNGG 3 cut(s) 94, 165, 809
BstSFI CTRYAG 1 cut(s) 1254
BstUI CGCG 2 cut(s) 155, 261
BstV1I GCAGC 3 cut(s) 145, 213, 1003
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 2 cut(s) 299, 1162
BsuRI GGCC 3 cut(s) 94, 617, 1238
BtgI CCRYGG 2 cut(s) 153, 1190
BtsCI GGATG 5 cut(s) 133, 153, 205, 271, 703
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 297
Cfr13I GGNCC 3 cut(s) 93, 616, 1149
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 3 cut(s) 218, 401, 737
Csp6I GTAC 1 cut(s) 1198
CviAII CATG 4 cut(s) 524, 532, 961, 1013
CviQI GTAC 1 cut(s) 1198
DdeI CTNAG 6 cut(s) 53, 186, 240, 511, 567, 585
DpnI GATC 6 cut(s) 219, 279, 366, 815, 1206, 1290
DpnII GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
EaeI YGGCCR 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 1149
Eco57I CTGAAG 1 cut(s) 563
Eco88I CYCGRG 2 cut(s) 107, 620
EcoRII CCWGG 2 cut(s) 165, 809
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 656
FaeI CATG 4 cut(s) 527, 535, 964, 1016
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FatI CATG 4 cut(s) 523, 531, 960, 1012
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 156, 159, 227, 1017
FokI GGATG 5 cut(s) 140, 160, 212, 278, 710
Fsp4HI GCNGC 4 cut(s) 156, 159, 227, 1017
FspBI CTAG 1 cut(s) 222
GluI GCNGC 4 cut(s) 156, 159, 227, 1017
GsuI CTGGAG 2 cut(s) 188, 1089
HaeIII GGCC 3 cut(s) 94, 617, 1238
HapII CCGG 3 cut(s) 96, 292, 1239
HgaI GACGC 3 cut(s) 218, 401, 737
Hin1II CATG 4 cut(s) 527, 535, 964, 1016
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 4 cut(s) 491, 509, 706, 919
HpaII CCGG 3 cut(s) 96, 292, 1239
HphI GGTGA 2 cut(s) 19, 1117
Hpy166II GTNNAC 1 cut(s) 1194
Hpy188III TCNNGA 6 cut(s) 107, 188, 260, 368, 817, 1049
Hpy8I GTNNAC 1 cut(s) 1194
Hpy99I CGWCG 1 cut(s) 234
HpyCH4III ACNGT 2 cut(s) 31, 1024
HpyCH4IV ACGT 1 cut(s) 1196
HpyCH4V TGCA 6 cut(s) 353, 964, 1004, 1019, 1111, 1250
HpyF10VI GCNNNNNNNGC 5 cut(s) 292, 737, 794, 1117, 1235
HpyF3I CTNAG 6 cut(s) 53, 186, 240, 511, 567, 585
HpySE526I ACGT 1 cut(s) 1196
Hsp92II CATG 4 cut(s) 527, 535, 964, 1016
Ksp22I TGATCA 1 cut(s) 277
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
LmnI GCTCC 3 cut(s) 109, 421, 1066
Lsp1109I GCAGC 3 cut(s) 145, 213, 1003
LweI GCATC 6 cut(s) 112, 340, 361, 528, 991, 1098
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 1196
MaeIII GTNAC 1 cut(s) 7
MalI GATC 6 cut(s) 219, 279, 366, 815, 1206, 1290
MboI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
MfeI CAATTG 2 cut(s) 315, 579
MflI RGATCY 1 cut(s) 364
MluCI AATT 8 cut(s) 63, 303, 315, 579, 597, 641, 1038, 1280
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 3 cut(s) 184, 1006, 1088
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 3 cut(s) 81, 399, 765
MslI CAYNNNNRTG 2 cut(s) 536, 981
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 96, 292, 1239
MspR9I CCNGG 3 cut(s) 96, 167, 811
MunI CAATTG 2 cut(s) 315, 579
Mva1269I GAATGC 1 cut(s) 1252
MvaI CCWGG 2 cut(s) 167, 811
MvnI CGCG 2 cut(s) 155, 261
MwoI GCNNNNNNNGC 5 cut(s) 292, 737, 794, 1117, 1235
NciI CCSGG 1 cut(s) 96
NdeII GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
NlaIII CATG 4 cut(s) 527, 535, 964, 1016
NlaIV GGNNCC 2 cut(s) 366, 1151
NmuCI GTSAC 1 cut(s) 7
NruI TCGCGA 1 cut(s) 261
OliI CACNNNNGTG 1 cut(s) 981
PaeR7I CTCGAG 1 cut(s) 620
PaqCI CACCTGC 1 cut(s) 5
PctI GAATGC 1 cut(s) 1252
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 3 cut(s) 491, 706, 919
PkrI GCNGC 4 cut(s) 157, 160, 228, 1018
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 165, 809
PspGI CCWGG 2 cut(s) 165, 809
PspN4I GGNNCC 2 cut(s) 366, 1151
PspPI GGNCC 3 cut(s) 93, 616, 1149
PspXI VCTCGAGB 1 cut(s) 620
PsuI RGATCY 1 cut(s) 364
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 1 cut(s) 1199
RsaNI GTAC 1 cut(s) 1198
RseI CAYNNNNRTG 2 cut(s) 536, 981
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 3 cut(s) 81, 399, 765
SatI GCNGC 4 cut(s) 156, 159, 227, 1017
Sau3AI GATC 6 cut(s) 217, 277, 364, 813, 1204, 1288
Sau96I GGNCC 3 cut(s) 93, 616, 1149
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 96, 167, 811
SfaNI GCATC 6 cut(s) 112, 340, 361, 528, 991, 1098
SfcI CTRYAG 1 cut(s) 1254
Sfr274I CTCGAG 1 cut(s) 620
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 1 cut(s) 1149
SlaI CTCGAG 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 536, 981
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 8 cut(s) 63, 303, 315, 579, 597, 641, 1038, 1280
SsiI CCGC 3 cut(s) 125, 153, 155
SspI AATATT 1 cut(s) 971
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 94, 165, 809
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 2 cut(s) 31, 1024
TaiI ACGT 1 cut(s) 1199
TaqI TCGA 2 cut(s) 621, 937
TasI AATT 8 cut(s) 63, 303, 315, 579, 597, 641, 1038, 1280
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 3 cut(s) 491, 706, 919
Tru1I TTAA 3 cut(s) 81, 399, 765
Tru9I TTAA 3 cut(s) 81, 399, 765
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 3 cut(s) 158, 226, 1016
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 6 cut(s) 144, 714, 774, 798, 949, 1259
VpaK11BI GGWCC 1 cut(s) 1149
XapI RAATTY 1 cut(s) 1038
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XhoI CTCGAG 1 cut(s) 620
XmaJI CCTAGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.