Rh2AG279700

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002954
Physical Location & Seq
Reverse (-)
33744489 .. 33746870
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG279700.1

Sequence Viewer

Length: 1299 bp
ATGTCGGTGACAGCAGGTGTTAGTGATACTGTGATTGCAATTAGGGATAAGCTGAGAGGTAAAATTGGACAAACTAAAGTTAAAAGGTATTGGCCCGGAAAAGCTCCCGAGTGGGGAGATGATGCGGATGAAGATGGCGATATTAGGATGTCCGCGGCTGCCTCCCTGGAGAAAGCCTTTCCCACTCAGGATTATTCGGATGTTGGAAGGAAAGATGATCCTAGGCTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGCGAGGATGTTAGGGCTGATCATAGGCGTATCCGGCAAGCTGAAATTGTTTCAACAATTGAAGAGGAAGCTAAGAGGCAGGAAGGGATAGATGCGGAAGAAGAGGATCCTGATGCTTTAGAGGAAAGGAGGAGAAGGATTAAGGAAAGGTTGCGTCAGAGGGAGCAAGAAGAAGCTCCACTCCTCCCCTCGGAAGATGAGGAAGAAGATAAGGAGGAGGAAGAGGAATCAGAGTATTCAACTGACTCAGAAGATGAGCATGGTGGCATGATGATGCTGAAGCCAGTCTTTGTTCCGAAGGCTGAGAGAGATACAATTGCTGAACGTGAGCGAATTGAGGCAGAAGAGTTGGCCCTCGAGGAAGCAAGGAAGAAAAAATTGGAGGAGAGGAAGAGAGAGACGAAGCAGATTGTGGTTGAGGAAATCCGAAAGGATGAAGAGATTCAGAAGGGTATGGAACAGGATGCTAACATTGCTGATATTGATACTGATGATGAACTTAATGAGGCAGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCAGGATCAAGAGGGATAGAGAGGAGAGGGAAGCTATGTTGAAGGAAAAGGAAGAGATAGAAAGAGTAAGAAATATGACAGAGGAAGAGAGGAGGGAATGGGAGAGGAAGAATCCTAAACCTGCACCTCGACCAAAGCAAAAGTGGAGGTTCATGCAGAAATATTACCACAAAGGTGCTTTCTTCCAGTCAGATGCAGATGACCACGCTGCAACTGTTGGAACAGATGGAATTTTCACTCGTGATTTCTCTGCTCCAACTGGAGAAGATAAGATGGATAAAACTATATTGCCGAAGGTCATGCAAGTCAAGCATTTTGGTCGTAGTGGAAGGACAAAATGGACCCATCTTGTCAATGAGGATACTACTGACTGGAACAATCCGTGGACCTACAATGATCCTCTTCGGGCAAAGTATAATGCTAAAATGGCCGGAATGAATGCACCTATAGAAAAACCCAGAGGACGCAAGAAATTGAAGGATCAGAAGTATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

432

Amino Acids

50.9

Weight (kDa)

5.13

Isoelectric Point (pI)

59.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 168 - 389 2.5e-77 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
AbsI CCTCGAGG 1 cut(s) 614
Acc36I ACCTGC 2 cut(s) 5, 931
AccII CGCG 2 cut(s) 155, 261
AciI CCGC 4 cut(s) 125, 153, 155, 353
AclWI GGATC 6 cut(s) 212, 359, 372, 815, 1193, 1290
AcoI YGGCCR 1 cut(s) 1230
AcsI RAATTY 1 cut(s) 1032
AcuI CTGAAG 1 cut(s) 557
AfiI CCNNNNNNNGG 2 cut(s) 113, 448
AgsI TTSAA 5 cut(s) 312, 320, 498, 844, 1279
AjnI CCWGG 2 cut(s) 165, 803
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AleI CACNNNNGTG 1 cut(s) 975
AluBI AGCT 7 cut(s) 52, 104, 299, 329, 434, 782, 836
AluI AGCT 7 cut(s) 52, 104, 299, 329, 434, 782, 836
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 6 cut(s) 212, 359, 372, 815, 1193, 1290
Ama87I CYCGRG 2 cut(s) 107, 614
AoxI GGCC 3 cut(s) 92, 609, 1230
ApeKI GCWGC 3 cut(s) 158, 226, 1010
ApoI RAATTY 1 cut(s) 1032
Asp700I GAANNNNTTC 1 cut(s) 699
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 4 cut(s) 93, 610, 1143, 1188
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 2 cut(s) 107, 614
AvaII GGWCC 2 cut(s) 1143, 1188
AvrII CCTAGG 1 cut(s) 221
BamHI GGATCC 1 cut(s) 364
BauI CACGAG 1 cut(s) 1041
BbvI GCAGC 3 cut(s) 145, 213, 997
BccI CCATC 4 cut(s) 128, 1022, 1069, 1155
BcgI CGANNNNNNTGC 4 cut(s) 1084, 1103, 1118, 1137
BciT130I CCWGG 2 cut(s) 167, 805
BciVI GTATCC 2 cut(s) 299, 1156
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 1248
BfuAI ACCTGC 2 cut(s) 5, 931
BfuI GTATCC 2 cut(s) 299, 1156
BisI GCNGC 4 cut(s) 156, 159, 227, 1011
BlnI CCTAGG 1 cut(s) 221
BlsI GCNGC 4 cut(s) 157, 160, 228, 1012
Bme1390I CCNGG 3 cut(s) 96, 167, 805
Bme18I GGWCC 2 cut(s) 1143, 1188
BmeT110I CYCGRG 2 cut(s) 107, 614
BmgT120I GGNCC 4 cut(s) 93, 610, 1143, 1188
BmiI GGNNCC 2 cut(s) 366, 1145
BmrFI CCNGG 3 cut(s) 96, 167, 805
BmsI GCATC 6 cut(s) 112, 340, 361, 522, 712, 985
BpmI CTGGAG 2 cut(s) 188, 1083
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 5 cut(s) 153, 165, 221, 447, 1184
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 448
Bse1I ACTGG 4 cut(s) 542, 988, 1066, 1178
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 2 cut(s) 167, 805
BseDI CCNNGG 5 cut(s) 153, 165, 221, 447, 1184
BseGI GGATG 6 cut(s) 133, 153, 205, 271, 697, 727
BseLI CCNNNNNNNGG 2 cut(s) 113, 448
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 5 cut(s) 44, 200, 231, 519, 552
BseNI ACTGG 4 cut(s) 542, 988, 1066, 1178
BseRI GAGGAG 7 cut(s) 403, 431, 488, 656, 785, 839, 907
BseXI GCAGC 3 cut(s) 145, 213, 997
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 2 cut(s) 155, 261
BshFI GGCC 3 cut(s) 94, 611, 1232
BsiHKCI CYCGRG 2 cut(s) 107, 614
BsiSI CCGG 3 cut(s) 96, 292, 1233
BslI CCNNNNNNNGG 2 cut(s) 113, 448
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmI GAATGC 1 cut(s) 1246
BsnI GGCC 3 cut(s) 94, 611, 1232
BsoBI CYCGRG 2 cut(s) 107, 614
Bsp143I GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 4 cut(s) 125, 153, 155, 353
BspANI GGCC 3 cut(s) 94, 611, 1232
BspCNI CTCAG 5 cut(s) 45, 199, 232, 518, 553
BspFNI CGCG 2 cut(s) 155, 261
BspLI GGNNCC 2 cut(s) 366, 1145
BspMI ACCTGC 2 cut(s) 5, 931
BspPI GGATC 6 cut(s) 212, 359, 372, 815, 1193, 1290
BsrDI GCAATG 1 cut(s) 729
BsrI ACTGG 4 cut(s) 542, 988, 1066, 1178
BssECI CCNNGG 5 cut(s) 153, 165, 221, 447, 1184
BssMI GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
BssSI CACGAG 1 cut(s) 1041
BssT1I CCWWGG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 1041
Bst2UI CCWGG 2 cut(s) 167, 805
Bst4CI ACNGT 2 cut(s) 31, 1018
Bst6I CTCTTC 9 cut(s) 315, 354, 474, 597, 644, 690, 849, 882, 1209
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 6 cut(s) 53, 186, 240, 330, 505, 561
BstDSI CCRYGG 2 cut(s) 153, 1184
BstF5I GGATG 6 cut(s) 133, 153, 205, 271, 697, 727
BstFNI CGCG 2 cut(s) 155, 261
BstKTI GATC 6 cut(s) 220, 280, 367, 810, 1201, 1285
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
BstMWI GCNNNNNNNGC 5 cut(s) 292, 731, 788, 1111, 1229
BstNI CCWGG 2 cut(s) 167, 805
BstSCI CCNGG 3 cut(s) 94, 165, 803
BstSFI CTRYAG 1 cut(s) 1248
BstUI CGCG 2 cut(s) 155, 261
BstV1I GCAGC 3 cut(s) 145, 213, 997
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 2 cut(s) 299, 1156
BsuRI GGCC 3 cut(s) 94, 611, 1232
BtgI CCRYGG 2 cut(s) 153, 1184
BtsCI GGATG 6 cut(s) 133, 153, 205, 271, 697, 727
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 2 cut(s) 5, 931
Cac8I GCNNGC 1 cut(s) 297
Cfr13I GGNCC 4 cut(s) 93, 610, 1143, 1188
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 3 cut(s) 218, 401, 1275
CviAII CATG 4 cut(s) 518, 526, 955, 1102
DdeI CTNAG 6 cut(s) 53, 186, 240, 330, 505, 561
DpnI GATC 6 cut(s) 219, 279, 366, 809, 1200, 1284
DpnII GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
EaeI YGGCCR 1 cut(s) 1230
Eam1104I CTCTTC 9 cut(s) 315, 354, 474, 597, 644, 690, 849, 882, 1209
EarI CTCTTC 9 cut(s) 315, 354, 474, 597, 644, 690, 849, 882, 1209
Eco130I CCWWGG 1 cut(s) 221
Eco47I GGWCC 2 cut(s) 1143, 1188
Eco57I CTGAAG 1 cut(s) 557
Eco88I CYCGRG 2 cut(s) 107, 614
EcoRII CCWGG 2 cut(s) 165, 803
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 4 cut(s) 521, 529, 958, 1105
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FatI CATG 4 cut(s) 517, 525, 954, 1101
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 156, 159, 227, 1011
FokI GGATG 6 cut(s) 140, 160, 212, 278, 704, 734
Fsp4HI GCNGC 4 cut(s) 156, 159, 227, 1011
FspBI CTAG 1 cut(s) 222
GluI GCNGC 4 cut(s) 156, 159, 227, 1011
GsuI CTGGAG 2 cut(s) 188, 1083
HaeIII GGCC 3 cut(s) 94, 611, 1232
HapII CCGG 3 cut(s) 96, 292, 1233
HgaI GACGC 3 cut(s) 218, 401, 1275
Hin1II CATG 4 cut(s) 521, 529, 958, 1105
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 4 cut(s) 485, 503, 700, 913
HpaII CCGG 3 cut(s) 96, 292, 1233
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 1188
Hpy188III TCNNGA 6 cut(s) 107, 188, 260, 368, 811, 1043
Hpy8I GTNNAC 1 cut(s) 1188
Hpy99I CGWCG 1 cut(s) 234
HpyCH4III ACNGT 2 cut(s) 31, 1018
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 7 cut(s) 38, 926, 958, 998, 1013, 1105, 1244
HpyF10VI GCNNNNNNNGC 5 cut(s) 292, 731, 788, 1111, 1229
HpyF3I CTNAG 6 cut(s) 53, 186, 240, 330, 505, 561
HpySE526I ACGT 1 cut(s) 583
Hsp92II CATG 4 cut(s) 521, 529, 958, 1105
Ksp22I TGATCA 1 cut(s) 277
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
LmnI GCTCC 4 cut(s) 109, 421, 439, 1060
Lsp1109I GCAGC 3 cut(s) 145, 213, 997
LweI GCATC 6 cut(s) 112, 340, 361, 522, 712, 985
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 1 cut(s) 7
MalI GATC 6 cut(s) 219, 279, 366, 809, 1200, 1284
MboI GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
MfeI CAATTG 2 cut(s) 315, 573
MflI RGATCY 1 cut(s) 364
MluCI AATT 9 cut(s) 39, 63, 303, 315, 573, 591, 635, 1032, 1274
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 3 cut(s) 184, 1000, 1082
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 3 cut(s) 81, 399, 759
MslI CAYNNNNRTG 2 cut(s) 530, 975
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 96, 292, 1233
MspR9I CCNGG 3 cut(s) 96, 167, 805
MunI CAATTG 2 cut(s) 315, 573
Mva1269I GAATGC 1 cut(s) 1246
MvaI CCWGG 2 cut(s) 167, 805
MvnI CGCG 2 cut(s) 155, 261
MwoI GCNNNNNNNGC 5 cut(s) 292, 731, 788, 1111, 1229
NciI CCSGG 1 cut(s) 96
NdeII GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
NlaIII CATG 4 cut(s) 521, 529, 958, 1105
NlaIV GGNNCC 2 cut(s) 366, 1145
NmuCI GTSAC 1 cut(s) 7
NruI TCGCGA 1 cut(s) 261
OliI CACNNNNGTG 1 cut(s) 975
PaeR7I CTCGAG 1 cut(s) 614
PaqCI CACCTGC 1 cut(s) 5
PctI GAATGC 1 cut(s) 1246
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 3 cut(s) 485, 700, 913
PkrI GCNGC 4 cut(s) 157, 160, 228, 1012
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
Psp6I CCWGG 2 cut(s) 165, 803
PspGI CCWGG 2 cut(s) 165, 803
PspN4I GGNNCC 2 cut(s) 366, 1145
PspPI GGNCC 4 cut(s) 93, 610, 1143, 1188
PspXI VCTCGAGB 1 cut(s) 614
PsuI RGATCY 1 cut(s) 364
RruI TCGCGA 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 530, 975
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 3 cut(s) 81, 399, 759
SatI GCNGC 4 cut(s) 156, 159, 227, 1011
Sau3AI GATC 6 cut(s) 217, 277, 364, 807, 1198, 1282
Sau96I GGNCC 4 cut(s) 93, 610, 1143, 1188
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 3 cut(s) 96, 167, 805
SfaNI GCATC 6 cut(s) 112, 340, 361, 522, 712, 985
SfcI CTRYAG 1 cut(s) 1248
Sfr274I CTCGAG 1 cut(s) 614
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 2 cut(s) 1143, 1188
SlaI CTCGAG 1 cut(s) 614
SmiMI CAYNNNNRTG 2 cut(s) 530, 975
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 9 cut(s) 39, 63, 303, 315, 573, 591, 635, 1032, 1274
SsiI CCGC 4 cut(s) 125, 153, 155, 353
SspI AATATT 1 cut(s) 965
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 94, 165, 803
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 2 cut(s) 31, 1018
TaiI ACGT 1 cut(s) 586
TaqI TCGA 2 cut(s) 615, 931
TasI AATT 9 cut(s) 39, 63, 303, 315, 573, 591, 635, 1032, 1274
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 3 cut(s) 485, 700, 913
Tru1I TTAA 3 cut(s) 81, 399, 759
Tru9I TTAA 3 cut(s) 81, 399, 759
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 3 cut(s) 158, 226, 1010
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 6 cut(s) 144, 708, 768, 792, 943, 1253
TspGWI ACGGA 1 cut(s) 1173
VpaK11BI GGWCC 2 cut(s) 1143, 1188
XapI RAATTY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 942
XhoI CTCGAG 1 cut(s) 614
XmaJI CCTAGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.