Rh2DG305600

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002954
Physical Location & Seq
Reverse (-)
37100179 .. 37103836
3658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG305600.1

Sequence Viewer

Length: 1203 bp
ATGTCGGTGACAGCAGGTGTTAGTGATACTGTGATTGCAATTAGGGATAAGCTGAGAGGTAAAATTGGACAAACTAAAGTTAAAAGGTATTGGCCCGGAAAAGCTCCCGAGTGGGGAGATGATGCGGATGAAGATGGCGATATTAGGATGTCCGCGGCTGCCTCCCTGGAGAAAGCCTTTCCCACTCAGGATTATTCGGATGTTGGAAGGAAAGATGATCCTAGGCTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGCGAGGATGTTAGGGCTGATCATAGGCGTATCCGGCAAGCTGAAATTGTTTCAACAATTGAAGAGGAAGCTAAGAGGCAGGAAGGGATAGATGCGGAAGAAGAGGATCCTGATGCTTTAGAGGAAAGGAGGAGAAGGATTAAGGAAAGGTTGCGTCAGAGGGAGCAAGAAGAAGCTCCACTCCTCCCCTCGGAAGATGAGGAAGAAGATAAGGAAGAGGAGGAGGAAGAGGAATCAGAGTATTCAACTGACTCAGAAGATGAGCATGGTGGCATGATGATGCTGAAGCCAGTCTTTGTTCCGAAGGCTGAGAGAGATACAATTGCTGAACGTGAGCGAATTGAGGCAGAAGAGTTGGCCCTCGAGGAAGCAAGGAAGAAAAAATTGGAGGAGAGGAAGAGAGAGACAAAGCAGATTGTGGTTGAGGAAATTCGAAAGGATGAAGAGATTCAGAAGGGTATGGAACAGGACGCTAACATTGCTGATATTGATACTGATGATGAACTTAATGAGGCAGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCAGGATCAAGAGGGATAGAGAGGAGAGGGAAGCTATGTTGAAGGAAAAGGAAGAGATAGAAAGAGTAAGAAATATGACAGAGGAAGAGAGGAGGGAATGGGAGAGGAAGAATCCTAAACCTGCACCTCGACCAAAGCAAAAGTGGAGGTTCATGCAGAAATATTACCACAAAGGTGCTTTCTTCCAGTCAGATGCAGATGACCACGCTGCAACTGTTGGAACAGATGGAATTTTCACTCGTGATTTCTCTGCTCCAACTGGAGAAGATAAGATGGATAAAACTATTTTGCCGAAGGTCATGCAAGTCAAGCATTTTGGTCGTAGTGGAAGGACAAAATGGACCCATCTTGTCAATGAGGATACTACTGACTGGAACAATCCTATTGTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

400

Amino Acids

47.06

Weight (kDa)

4.88

Isoelectric Point (pI)

62.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 170 - 391 2.2e-77 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
AbsI CCTCGAGG 1 cut(s) 620
Acc36I ACCTGC 2 cut(s) 5, 937
AccII CGCG 2 cut(s) 155, 261
AciI CCGC 4 cut(s) 125, 153, 155, 353
AclWI GGATC 4 cut(s) 212, 359, 372, 821
AcsI RAATTY 2 cut(s) 687, 1038
AcuI CTGAAG 1 cut(s) 563
AfiI CCNNNNNNNGG 2 cut(s) 113, 448
AgsI TTSAA 4 cut(s) 312, 320, 504, 850
AjnI CCWGG 2 cut(s) 165, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AleI CACNNNNGTG 1 cut(s) 981
AluBI AGCT 7 cut(s) 52, 104, 299, 329, 434, 788, 842
AluI AGCT 7 cut(s) 52, 104, 299, 329, 434, 788, 842
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 4 cut(s) 212, 359, 372, 821
Ama87I CYCGRG 2 cut(s) 107, 620
AoxI GGCC 2 cut(s) 92, 615
ApeKI GCWGC 3 cut(s) 158, 226, 1016
ApoI RAATTY 2 cut(s) 687, 1038
Asp700I GAANNNNTTC 1 cut(s) 705
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 93, 616, 1149
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 19
AsuII TTCGAA 1 cut(s) 691
AvaI CYCGRG 2 cut(s) 107, 620
AvaII GGWCC 1 cut(s) 1149
AvrII CCTAGG 1 cut(s) 221
BamHI GGATCC 1 cut(s) 364
BauI CACGAG 1 cut(s) 1047
BbvI GCAGC 3 cut(s) 145, 213, 1003
BccI CCATC 4 cut(s) 128, 1028, 1075, 1161
BcgI CGANNNNNNTGC 4 cut(s) 1090, 1109, 1124, 1143
BciT130I CCWGG 2 cut(s) 167, 811
BciVI GTATCC 2 cut(s) 299, 1162
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 1 cut(s) 222
BfuAI ACCTGC 2 cut(s) 5, 937
BfuI GTATCC 2 cut(s) 299, 1162
BisI GCNGC 4 cut(s) 156, 159, 227, 1017
BlnI CCTAGG 1 cut(s) 221
BlsI GCNGC 4 cut(s) 157, 160, 228, 1018
Bme1390I CCNGG 3 cut(s) 96, 167, 811
Bme18I GGWCC 1 cut(s) 1149
BmeT110I CYCGRG 2 cut(s) 107, 620
BmgT120I GGNCC 3 cut(s) 93, 616, 1149
BmiI GGNNCC 2 cut(s) 366, 1151
BmrFI CCNGG 3 cut(s) 96, 167, 811
BmsI GCATC 5 cut(s) 112, 340, 361, 528, 991
BpmI CTGGAG 2 cut(s) 188, 1089
Bpu14I TTCGAA 1 cut(s) 691
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 153, 165, 221, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 448
Bse1I ACTGG 4 cut(s) 548, 994, 1072, 1184
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 167, 811
BseDI CCNNGG 4 cut(s) 153, 165, 221, 447
BseGI GGATG 5 cut(s) 133, 153, 205, 271, 703
BseLI CCNNNNNNNGG 2 cut(s) 113, 448
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 5 cut(s) 44, 200, 231, 525, 558
BseNI ACTGG 4 cut(s) 548, 994, 1072, 1184
BseRI GAGGAG 8 cut(s) 403, 431, 491, 494, 662, 791, 845, 913
BseXI GCAGC 3 cut(s) 145, 213, 1003
BsgI GTGCAG 1 cut(s) 915
Bsh1236I CGCG 2 cut(s) 155, 261
BshFI GGCC 2 cut(s) 94, 617
BsiHKCI CYCGRG 2 cut(s) 107, 620
BsiSI CCGG 2 cut(s) 96, 292
BslI CCNNNNNNNGG 2 cut(s) 113, 448
BsmAI GTCTC 1 cut(s) 656
BsnI GGCC 2 cut(s) 94, 617
BsoBI CYCGRG 2 cut(s) 107, 620
Bsp119I TTCGAA 1 cut(s) 691
Bsp143I GATC 4 cut(s) 217, 277, 364, 813
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 4 cut(s) 125, 153, 155, 353
BspANI GGCC 2 cut(s) 94, 617
BspCNI CTCAG 5 cut(s) 45, 199, 232, 524, 559
BspFNI CGCG 2 cut(s) 155, 261
BspLI GGNNCC 2 cut(s) 366, 1151
BspMI ACCTGC 2 cut(s) 5, 937
BspPI GGATC 4 cut(s) 212, 359, 372, 821
BspT104I TTCGAA 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 735
BsrI ACTGG 4 cut(s) 548, 994, 1072, 1184
BssECI CCNNGG 4 cut(s) 153, 165, 221, 447
BssMI GATC 4 cut(s) 217, 277, 364, 813
BssSI CACGAG 1 cut(s) 1047
BssT1I CCWWGG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 1047
Bst2UI CCWGG 2 cut(s) 167, 811
Bst4CI ACNGT 2 cut(s) 31, 1024
Bst6I CTCTTC 9 cut(s) 315, 354, 468, 480, 603, 650, 696, 855, 888
BstBI TTCGAA 1 cut(s) 691
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 6 cut(s) 53, 186, 240, 330, 511, 567
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 5 cut(s) 133, 153, 205, 271, 703
BstFNI CGCG 2 cut(s) 155, 261
BstKTI GATC 4 cut(s) 220, 280, 367, 816
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 4 cut(s) 217, 277, 364, 813
BstMWI GCNNNNNNNGC 4 cut(s) 292, 737, 794, 1117
BstNI CCWGG 2 cut(s) 167, 811
BstSCI CCNGG 3 cut(s) 94, 165, 809
BstUI CGCG 2 cut(s) 155, 261
BstV1I GCAGC 3 cut(s) 145, 213, 1003
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 2 cut(s) 299, 1162
BsuRI GGCC 2 cut(s) 94, 617
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 5 cut(s) 133, 153, 205, 271, 703
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 2 cut(s) 5, 937
Cac8I GCNNGC 1 cut(s) 297
Cfr13I GGNCC 3 cut(s) 93, 616, 1149
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 3 cut(s) 218, 401, 737
CviAII CATG 4 cut(s) 524, 532, 961, 1108
DdeI CTNAG 6 cut(s) 53, 186, 240, 330, 511, 567
DpnI GATC 4 cut(s) 219, 279, 366, 815
DpnII GATC 4 cut(s) 217, 277, 364, 813
Eam1104I CTCTTC 9 cut(s) 315, 354, 468, 480, 603, 650, 696, 855, 888
EarI CTCTTC 9 cut(s) 315, 354, 468, 480, 603, 650, 696, 855, 888
Eco130I CCWWGG 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 1149
Eco57I CTGAAG 1 cut(s) 563
Eco88I CYCGRG 2 cut(s) 107, 620
EcoRII CCWGG 2 cut(s) 165, 809
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
FaeI CATG 4 cut(s) 527, 535, 964, 1111
FaiI YATR 9 cut(s) 282, 525, 533, 719, 783, 845, 884, 962, 1109
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FatI CATG 4 cut(s) 523, 531, 960, 1107
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 156, 159, 227, 1017
FokI GGATG 5 cut(s) 140, 160, 212, 278, 710
Fsp4HI GCNGC 4 cut(s) 156, 159, 227, 1017
FspBI CTAG 1 cut(s) 222
GluI GCNGC 4 cut(s) 156, 159, 227, 1017
GsuI CTGGAG 2 cut(s) 188, 1089
HaeIII GGCC 2 cut(s) 94, 617
HapII CCGG 2 cut(s) 96, 292
HgaI GACGC 3 cut(s) 218, 401, 737
Hin1II CATG 4 cut(s) 527, 535, 964, 1111
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 4 cut(s) 491, 509, 706, 919
HpaII CCGG 2 cut(s) 96, 292
HphI GGTGA 1 cut(s) 19
Hpy188I TCNGA 8 cut(s) 199, 417, 451, 496, 514, 561, 711, 1000
Hpy188III TCNNGA 6 cut(s) 107, 188, 260, 368, 817, 1049
Hpy99I CGWCG 1 cut(s) 234
HpyAV CCTTC 9 cut(s) 201, 335, 387, 556, 706, 787, 844, 1096, 1131
HpyCH4III ACNGT 2 cut(s) 31, 1024
HpyCH4IV ACGT 1 cut(s) 589
HpyCH4V TGCA 6 cut(s) 38, 932, 964, 1004, 1019, 1111
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 737, 794, 1117
HpyF3I CTNAG 6 cut(s) 53, 186, 240, 330, 511, 567
HpySE526I ACGT 1 cut(s) 589
Hsp92II CATG 4 cut(s) 527, 535, 964, 1111
Ksp22I TGATCA 1 cut(s) 277
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 4 cut(s) 217, 277, 364, 813
LmnI GCTCC 4 cut(s) 109, 421, 439, 1066
Lsp1109I GCAGC 3 cut(s) 145, 213, 1003
LweI GCATC 5 cut(s) 112, 340, 361, 528, 991
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 589
MaeIII GTNAC 1 cut(s) 7
MalI GATC 4 cut(s) 219, 279, 366, 815
MboI GATC 4 cut(s) 217, 277, 364, 813
MfeI CAATTG 2 cut(s) 315, 579
MflI RGATCY 1 cut(s) 364
MluCI AATT 9 cut(s) 39, 63, 303, 315, 579, 597, 641, 687, 1038
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 3 cut(s) 184, 1006, 1088
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 3 cut(s) 81, 399, 765
MslI CAYNNNNRTG 2 cut(s) 536, 981
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 2 cut(s) 96, 292
MspR9I CCNGG 3 cut(s) 96, 167, 811
MunI CAATTG 2 cut(s) 315, 579
MvaI CCWGG 2 cut(s) 167, 811
MvnI CGCG 2 cut(s) 155, 261
MwoI GCNNNNNNNGC 4 cut(s) 292, 737, 794, 1117
NciI CCSGG 1 cut(s) 96
NdeII GATC 4 cut(s) 217, 277, 364, 813
NlaIII CATG 4 cut(s) 527, 535, 964, 1111
NlaIV GGNNCC 2 cut(s) 366, 1151
NmuCI GTSAC 1 cut(s) 7
NruI TCGCGA 1 cut(s) 261
NspV TTCGAA 1 cut(s) 691
OliI CACNNNNGTG 1 cut(s) 981
PaeR7I CTCGAG 1 cut(s) 620
PaqCI CACCTGC 1 cut(s) 5
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 3 cut(s) 491, 706, 919
PkrI GCNGC 4 cut(s) 157, 160, 228, 1018
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 165, 809
PspGI CCWGG 2 cut(s) 165, 809
PspN4I GGNNCC 2 cut(s) 366, 1151
PspPI GGNCC 3 cut(s) 93, 616, 1149
PspXI VCTCGAGB 1 cut(s) 620
PsuI RGATCY 1 cut(s) 364
RruI TCGCGA 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 536, 981
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 3 cut(s) 81, 399, 765
SatI GCNGC 4 cut(s) 156, 159, 227, 1017
Sau3AI GATC 4 cut(s) 217, 277, 364, 813
Sau96I GGNCC 3 cut(s) 93, 616, 1149
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 96, 167, 811
SfaNI GCATC 5 cut(s) 112, 340, 361, 528, 991
Sfr274I CTCGAG 1 cut(s) 620
Sfr303I CCGCGG 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 691
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 1 cut(s) 1149
SlaI CTCGAG 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 536, 981
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 9 cut(s) 39, 63, 303, 315, 579, 597, 641, 687, 1038
SsiI CCGC 4 cut(s) 125, 153, 155, 353
SspI AATATT 1 cut(s) 971
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 94, 165, 809
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 2 cut(s) 31, 1024
TaiI ACGT 1 cut(s) 592
TaqI TCGA 3 cut(s) 621, 691, 937
TasI AATT 9 cut(s) 39, 63, 303, 315, 579, 597, 641, 687, 1038
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 3 cut(s) 491, 706, 919
Tru1I TTAA 3 cut(s) 81, 399, 765
Tru9I TTAA 3 cut(s) 81, 399, 765
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 3 cut(s) 158, 226, 1016
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 5 cut(s) 144, 714, 774, 798, 949
VpaK11BI GGWCC 1 cut(s) 1149
XapI RAATTY 2 cut(s) 687, 1038
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XhoI CTCGAG 1 cut(s) 620
XmaJI CCTAGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.