Rh7AG356300

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002954
Physical Location & Seq
Reverse (-)
45638821 .. 45643328
4508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG356300.1

Sequence Viewer

Length: 1197 bp
ATGTCGGTGACTGCAGGTGTTAGTGACACTGTCATTGCCATTAGGGATAAGCTGAGAGGTAAAATTGGACAGACTAAAGTTAAAAGGTATTGGCCTGGAAAAGCTCCTGAGTGGGGAGATGATGCCGACGAAGATGGCGATATTAGGTTGTCCGCTGCTGCCTCCCTCGAGAAAGCCTTCCCCACTCAGGATTATTCCGATATTGCAAGGAAAGATGATCCTAGGCTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGCGACGATGTTAGGGCTGATCACAGGCGTATCCGCCAAGCTGAAATTGTTTCAACTATTGAAGAGGAAGCCAGGAGGCAGGAAGGGATAGATGCCGAAGAAGAGGATCCTGATGCTTTAGAGGAAAGGAGAAGAAGGATTAAGGAAAGGTTGCGTGAGAGGGAGCAAGAAGAAGCTGCACTCCTCCCCTCCGAAGATGAGGAAGAGGATAAGGAAGAAGAGGAGGAGGAAGAGGAATCAGAGTATTCAACTGACTCAGAAGATGAGCATGGTGGTATGATGATGCTCAAGCCAGTCTTTGTTCCCAAGGCTGAGAGAGATACCATTGCTGAACGTGAGCGAATTGAGGCAGAAGAACTGGCCCTTGAGGAAGCAAGGAAGAAAAAATTGGAGGAGAGGAAGAGAGAGACAAAGCAGATTGTGGTTGAGGAAATCCGAAAGGATGAAGAGATACAGAAAAGTATGGAACAGGACGCTAACATTGCTGATATTGATACTGATGATGAACTTAATGAGGCAGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCAGGATCAAGAGGGATAGGGAGGAAAGAGAAGCGATGTTGAAGGAAAAGGAAGAGATTGAAAGAGTAAGAAATATGACAGAGGAAGAGAGGAGGGAATGGGAGAGGAAGAATCCTAAACCTGCACCAAAACCAAAGCAAAAGTGGAGGTTCATGCAGAAGTACCACCACAAAGGTGCTTTCTTCCAGTCAGATGCAGATGACCATGCTGCAACTGTGGGAACAGATGGAATTTTTACTCGTGATTTCTCTGCTCCAACTGGAGAAGATAAAATGGACAAAACTATATTGCCGAAGGTCATGCAAGTCAAGCATTTTGGTCGTAGTGGAAGGACAAAATGGACCCATCTTGTCAATGAGGATACTACTGACTGGAACAATCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

398

Amino Acids

46.83

Weight (kDa)

4.87

Isoelectric Point (pI)

61.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 171 - 392 5.1e-77 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
Acc36I ACCTGC 2 cut(s) 5, 940
AccII CGCG 1 cut(s) 261
AciI CCGC 2 cut(s) 153, 292
AclWI GGATC 4 cut(s) 212, 359, 372, 824
AcsI RAATTY 1 cut(s) 1041
AfaI GTAC 1 cut(s) 974
AfiI CCNNNNNNNGG 2 cut(s) 113, 187
AgsI TTSAA 5 cut(s) 312, 320, 507, 853, 872
AjnI CCWGG 3 cut(s) 94, 329, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 984
AluBI AGCT 5 cut(s) 52, 104, 299, 434, 791
AluI AGCT 5 cut(s) 52, 104, 299, 434, 791
Alw26I GTCTC 1 cut(s) 659
AlwI GGATC 4 cut(s) 212, 359, 372, 824
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 2 cut(s) 92, 618
ApeKI GCWGC 5 cut(s) 155, 158, 226, 434, 1019
ApoI RAATTY 1 cut(s) 1041
Asp700I GAANNNNTTC 1 cut(s) 176
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 619, 1152
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 1152
AvrII CCTAGG 1 cut(s) 221
BaeI ACNNNNGTAYC 2 cut(s) 271, 304
BamHI GGATCC 1 cut(s) 364
BauI CACGAG 1 cut(s) 1050
BbvI GCAGC 5 cut(s) 142, 145, 213, 421, 1006
BccI CCATC 3 cut(s) 128, 1031, 1164
BcgI CGANNNNNNTGC 4 cut(s) 1093, 1112, 1127, 1146
BciT130I CCWGG 3 cut(s) 96, 331, 814
BciVI GTATCC 2 cut(s) 299, 1165
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 659
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 12
BfuAI ACCTGC 2 cut(s) 5, 940
BfuI GTATCC 2 cut(s) 299, 1165
BisI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
BlnI CCTAGG 1 cut(s) 221
BlsI GCNGC 5 cut(s) 157, 160, 228, 436, 1021
Bme1390I CCNGG 3 cut(s) 96, 331, 814
Bme18I GGWCC 1 cut(s) 1152
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 619, 1152
BmiI GGNNCC 2 cut(s) 366, 1154
BmrFI CCNGG 3 cut(s) 96, 331, 814
BmsI GCATC 5 cut(s) 112, 340, 361, 531, 994
BpmI CTGGAG 1 cut(s) 1092
BpuEI CTTGAG 2 cut(s) 530, 644
BsaJI CCNNGG 2 cut(s) 221, 564
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 187
Bse1I ACTGG 5 cut(s) 551, 621, 997, 1075, 1187
Bse3DI GCAATG 3 cut(s) 33, 582, 738
BseBI CCWGG 3 cut(s) 96, 331, 814
BseDI CCNNGG 2 cut(s) 221, 564
BseGI GGATG 1 cut(s) 706
BseLI CCNNNNNNNGG 2 cut(s) 113, 187
BseMI GCAATG 3 cut(s) 33, 582, 738
BseMII CTCAG 6 cut(s) 44, 99, 200, 231, 528, 561
BseNI ACTGG 5 cut(s) 551, 621, 997, 1075, 1187
BseRI GAGGAG 6 cut(s) 431, 494, 497, 665, 794, 916
BseXI GCAGC 5 cut(s) 142, 145, 213, 421, 1006
BsgI GTGCAG 2 cut(s) 420, 918
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 2 cut(s) 94, 620
BsiHKCI CYCGRG 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 113, 187
BsmAI GTCTC 1 cut(s) 659
BsnI GGCC 2 cut(s) 94, 620
BsoBI CYCGRG 1 cut(s) 167
Bsp143I GATC 4 cut(s) 217, 277, 364, 816
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 2 cut(s) 153, 292
BspANI GGCC 2 cut(s) 94, 620
BspCNI CTCAG 6 cut(s) 45, 100, 199, 232, 527, 562
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 366, 1154
BspMAI CTGCAG 1 cut(s) 16
BspMI ACCTGC 2 cut(s) 5, 940
BspPI GGATC 4 cut(s) 212, 359, 372, 824
BsrDI GCAATG 3 cut(s) 33, 582, 738
BsrI ACTGG 5 cut(s) 551, 621, 997, 1075, 1187
BssECI CCNNGG 2 cut(s) 221, 564
BssMI GATC 4 cut(s) 217, 277, 364, 816
BssSI CACGAG 1 cut(s) 1050
BssT1I CCWWGG 2 cut(s) 221, 564
Bst2BI CACGAG 1 cut(s) 1050
Bst2UI CCWGG 3 cut(s) 96, 331, 814
Bst4CI ACNGT 2 cut(s) 31, 1027
Bst6I CTCTTC 9 cut(s) 315, 354, 456, 471, 483, 653, 699, 858, 891
BstDEI CTNAG 6 cut(s) 53, 108, 186, 240, 514, 570
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 1 cut(s) 261
BstKTI GATC 4 cut(s) 220, 280, 367, 819
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 4 cut(s) 217, 277, 364, 816
BstMWI GCNNNNNNNGC 3 cut(s) 740, 797, 1120
BstNI CCWGG 3 cut(s) 96, 331, 814
BstSCI CCNGG 3 cut(s) 94, 329, 812
BstSFI CTRYAG 1 cut(s) 12
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 5 cut(s) 142, 145, 213, 421, 1006
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 2 cut(s) 299, 1165
BsuRI GGCC 2 cut(s) 94, 620
BtgZI GCGATG 1 cut(s) 860
BtsCI GGATG 1 cut(s) 706
BtsIMutI CAGTG 1 cut(s) 27
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 2 cut(s) 5, 940
Cfr13I GGNCC 2 cut(s) 619, 1152
CseI GACGC 2 cut(s) 218, 740
Csp6I GTAC 1 cut(s) 973
CviAII CATG 4 cut(s) 527, 964, 1016, 1111
CviQI GTAC 1 cut(s) 973
DdeI CTNAG 6 cut(s) 53, 108, 186, 240, 514, 570
DpnI GATC 4 cut(s) 219, 279, 366, 818
DpnII GATC 4 cut(s) 217, 277, 364, 816
Eam1104I CTCTTC 9 cut(s) 315, 354, 456, 471, 483, 653, 699, 858, 891
EarI CTCTTC 9 cut(s) 315, 354, 456, 471, 483, 653, 699, 858, 891
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 221, 564
Eco47I GGWCC 1 cut(s) 1152
Eco88I CYCGRG 1 cut(s) 167
EcoRII CCWGG 3 cut(s) 94, 329, 812
EcoT14I CCWWGG 2 cut(s) 221, 564
ErhI CCWWGG 2 cut(s) 221, 564
FaeI CATG 4 cut(s) 530, 967, 1019, 1114
FaiI YATR 9 cut(s) 528, 536, 722, 786, 887, 965, 1017, 1097, 1112
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FatI CATG 4 cut(s) 526, 963, 1015, 1110
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
FokI GGATG 1 cut(s) 713
Fsp4HI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
FspBI CTAG 1 cut(s) 222
GluI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
GsuI CTGGAG 1 cut(s) 1092
HaeIII GGCC 2 cut(s) 94, 620
HgaI GACGC 2 cut(s) 218, 740
Hin1II CATG 4 cut(s) 530, 967, 1019, 1114
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 3 cut(s) 494, 512, 922
HphI GGTGA 1 cut(s) 19
Hpy188I TCNGA 6 cut(s) 199, 451, 499, 517, 695, 1003
Hpy188III TCNNGA 7 cut(s) 107, 169, 188, 260, 368, 820, 1052
Hpy99I CGWCG 3 cut(s) 131, 234, 266
HpyAV CCTTC 7 cut(s) 187, 335, 387, 790, 847, 1099, 1134
HpyCH4III ACNGT 2 cut(s) 31, 1027
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 8 cut(s) 14, 206, 437, 935, 967, 1007, 1022, 1114
HpyF10VI GCNNNNNNNGC 3 cut(s) 740, 797, 1120
HpyF3I CTNAG 6 cut(s) 53, 108, 186, 240, 514, 570
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 4 cut(s) 530, 967, 1019, 1114
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 4 cut(s) 217, 277, 364, 816
LmnI GCTCC 3 cut(s) 109, 421, 1069
Lsp1109I GCAGC 5 cut(s) 142, 145, 213, 421, 1006
LweI GCATC 5 cut(s) 112, 340, 361, 531, 994
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 2 cut(s) 7, 23
MalI GATC 4 cut(s) 219, 279, 366, 818
MboI GATC 4 cut(s) 217, 277, 364, 816
MflI RGATCY 1 cut(s) 364
MluCI AATT 5 cut(s) 63, 303, 600, 644, 1041
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 1091
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 3 cut(s) 81, 399, 768
MslI CAYNNNNRTG 1 cut(s) 984
MspA1I CMGCKG 1 cut(s) 155
MspR9I CCNGG 3 cut(s) 96, 331, 814
MvaI CCWGG 3 cut(s) 96, 331, 814
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 3 cut(s) 740, 797, 1120
NdeII GATC 4 cut(s) 217, 277, 364, 816
NlaIII CATG 4 cut(s) 530, 967, 1019, 1114
NlaIV GGNNCC 2 cut(s) 366, 1154
NmuCI GTSAC 2 cut(s) 7, 23
NruI TCGCGA 1 cut(s) 261
OliI CACNNNNGTG 1 cut(s) 984
PaeR7I CTCGAG 1 cut(s) 167
PaqCI CACCTGC 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 494, 922
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 5 cut(s) 157, 160, 228, 436, 1021
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 3 cut(s) 94, 329, 812
PspGI CCWGG 3 cut(s) 94, 329, 812
PspN4I GGNNCC 2 cut(s) 366, 1154
PspPI GGNCC 2 cut(s) 619, 1152
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 364
PsyI GACNNNGTC 1 cut(s) 29
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 1 cut(s) 974
RsaNI GTAC 1 cut(s) 973
RseI CAYNNNNRTG 1 cut(s) 984
SaqAI TTAA 3 cut(s) 81, 399, 768
SatI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
Sau3AI GATC 4 cut(s) 217, 277, 364, 816
Sau96I GGNCC 2 cut(s) 619, 1152
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 3 cut(s) 96, 331, 814
SfaNI GCATC 5 cut(s) 112, 340, 361, 531, 994
SfcI CTRYAG 1 cut(s) 12
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 1152
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 3 cut(s) 167, 545, 623
SmoI CTYRAG 3 cut(s) 167, 545, 623
Sse9I AATT 5 cut(s) 63, 303, 600, 644, 1041
SsiI CCGC 2 cut(s) 153, 292
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 94, 329, 812
StyI CCWWGG 2 cut(s) 221, 564
TaaI ACNGT 2 cut(s) 31, 1027
TaiI ACGT 1 cut(s) 595
TaqI TCGA 1 cut(s) 168
TasI AATT 5 cut(s) 63, 303, 600, 644, 1041
TfiI GAWTC 2 cut(s) 494, 922
Tru1I TTAA 3 cut(s) 81, 399, 768
Tru9I TTAA 3 cut(s) 81, 399, 768
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 2 cut(s) 7, 23
TseI GCWGC 5 cut(s) 155, 158, 226, 434, 1019
Tsp45I GTSAC 2 cut(s) 7, 23
TspDTI ATGAA 4 cut(s) 717, 777, 801, 952
TspGWI ACGGA 1 cut(s) 1182
TspRI CASTG 1 cut(s) 34
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 1152
XapI RAATTY 1 cut(s) 1041
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XhoI CTCGAG 1 cut(s) 167
XmaJI CCTAGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.