MD01G1001200.v1.1

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002954
Physical Location & Seq
Forward (+)
380513 .. 388436
7924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1001200.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGTCAGTAACAGCAGGTGTAAGTGATACTGTGATTGCAGTGAGGGATAAGCTCAGAGGTAAAATTGGGCAAACTAAGGTTAAAAGGTATTGGCCTGGAAAAGCTCCTGAGTGGGCAGATGATGCCGACGAGGATGGGGAGTTTAGGATGTCCAAGGTGCCCGGTTTTCCCACTCACGATTCTGACATTGTTAGGAAAGATGATCCTAGACTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGTGAGGATGTTAGAGCTGATCATAGGCGTATCCGGCAAGCTGAGATTGTTTCAACAATTGAAGAGGAAGCCAAGAGGCAGGAAGGGTTGGAAGCAGAGGAAGAGGACGCAGATGCCCAGGAAGAAAGAAGAAGGAAGATTAGGGAGAGGTTGCTTCAGAGGGAGCAAGAAGAACTCCTTCCCTCAGAAGATGAGGAAGATGTACAGGAAGAGGAGGAAGAGGAATCTGAGTATGATACTGACTCGGACGAAGAGCTTACTGGTATGGTGATGCTAAAGCCTGTCTTTGTTCCCAAGTCGGAGAGAGATACTATTGCTGAGCGTGAGCGACTTGAGGCTGAAGAATGGGCCCTTGAGGAATCTAGGAAGAAGAAATTGGCGGAACGGAAGAGAGAGACAAAGCAGATTGTGGTTGAGGAGATTCGGAAGGATGAAGAGATTCAGAAGGGTATGGAACTGGAAGCAAATATTGAAGATATTGATACTGATGATGAAGTAAACGAGGCAGAAGAGTATGATGCTTGGAAGGCCCGAGAGATTGCCAGGATCAAAAGGGATAGGGAAGATCGGGAAGCAATGTTGAAGGAAAAAGAAGAGATTGAAAGGGTCAGAAACATGACAGAAGAAGAGAGGAGGGAGTGGGAGAGAAAGAATCCAAGACCTGCACCACGACCAAAGCAGAAGTGGAGGTTCATGCAGAAATATTACCACAAAGGTGCTTTCTTCCAGTCAGATGCTGATGACTATGCAGCAACGGCTGGAACTGATGGAATTTACACACGTGATGTCTCTGCCCCAACTGGAGAAGATAAGATGGACAAAACAATATTACCCAAGGTCATGCAGGTCAAACATTTTGGTCGTAGTGGAAGGACGAAGTGGACCCATCTTGTCAATGAGGATACCACTGATTGGAACAATCCGTGGACATATAATGATCCTCTTCGGGCAAAGTATAATGCTAAAATGGCAGGAATGAATGTGCCTATTGCAAAACCCAAAGGAAGCAAGAAATTGAAGGATTGGGAGTCTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

427

Amino Acids

50.3

Weight (kDa)

5.01

Isoelectric Point (pI)

60.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 162 - 383 7.4e-76 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
Acc36I ACCTGC 3 cut(s) 5, 913, 1078
AccB1I GGYRCC 1 cut(s) 157
AccB7I CCANNNNNTGG 1 cut(s) 1155
AciI CCGC 1 cut(s) 623
AclWI GGATC 3 cut(s) 197, 797, 1175
AcsI RAATTY 1 cut(s) 1014
AcuI CTGAAG 2 cut(s) 383, 603
AcvI CACGTG 1 cut(s) 1025
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 1 cut(s) 1155
AflIII ACRYGT 1 cut(s) 1022
AgsI TTSAA 6 cut(s) 297, 305, 716, 826, 845, 1261
AjnI CCWGG 3 cut(s) 94, 360, 785
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AleI CACNNNNGTG 1 cut(s) 957
AluBI AGCT 5 cut(s) 52, 104, 260, 284, 499
AluI AGCT 5 cut(s) 52, 104, 260, 284, 499
Alw26I GTCTC 2 cut(s) 632, 1036
AlwI GGATC 3 cut(s) 197, 797, 1175
AlwNI CAGNNNCTG 1 cut(s) 980
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 3 cut(s) 92, 591, 771
ApaI GGGCCC 1 cut(s) 595
ApeKI GCWGC 1 cut(s) 992
ApoI RAATTY 1 cut(s) 1014
Asp700I GAANNNNTTC 2 cut(s) 420, 681
AspS9I GGNCC 4 cut(s) 591, 592, 772, 1125
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 1 cut(s) 1125
BaeGI GKGCMC 2 cut(s) 162, 595
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 595
BbrPI CACGTG 1 cut(s) 1025
BbvI GCAGC 1 cut(s) 1004
BccI CCATC 4 cut(s) 128, 1004, 1051, 1137
BceAI ACGGC 1 cut(s) 1014
BciT130I CCWGG 3 cut(s) 96, 362, 787
BciVI GTATCC 2 cut(s) 284, 1138
BclI TGATCA 1 cut(s) 262
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 632, 1036
BfaI CTAG 2 cut(s) 207, 606
BfuAI ACCTGC 3 cut(s) 5, 913, 1078
BfuI GTATCC 2 cut(s) 284, 1138
BisI GCNGC 1 cut(s) 993
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 994
Bme1390I CCNGG 4 cut(s) 96, 162, 362, 787
Bme18I GGWCC 1 cut(s) 1125
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 4 cut(s) 591, 592, 772, 1125
BmiI GGNNCC 3 cut(s) 159, 593, 1127
BmrFI CCNGG 4 cut(s) 96, 162, 362, 787
BmsI GCATC 5 cut(s) 112, 346, 504, 751, 967
BpmI CTGGAG 1 cut(s) 1065
Bpu1102I GCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 596, 617
BpuMI CCSGG 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 1025
BsaJI CCNNGG 4 cut(s) 153, 360, 1077, 1166
BsaXI ACNNNNNCTCC 1 cut(s) 1262
Bsc4I CCNNNNNNNGG 1 cut(s) 1155
Bse1I ACTGG 4 cut(s) 508, 705, 970, 1048
Bse3DI GCAATG 1 cut(s) 825
BseBI CCWGG 3 cut(s) 96, 362, 787
BseDI CCNNGG 4 cut(s) 153, 360, 1077, 1166
BseGI GGATG 4 cut(s) 139, 153, 256, 679
BseLI CCNNNNNNNGG 1 cut(s) 1155
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 7 cut(s) 67, 99, 216, 276, 441, 462, 552
BseNI ACTGG 4 cut(s) 508, 705, 970, 1048
BseRI GAGGAG 3 cut(s) 470, 674, 889
BseSI GKGCMC 2 cut(s) 162, 595
BseXI GCAGC 1 cut(s) 1004
BsgI GTGCAG 1 cut(s) 891
BshFI GGCC 3 cut(s) 94, 593, 773
BshNI GGYRCC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 2 cut(s) 162, 277
BslI CCNNNNNNNGG 1 cut(s) 1155
BsmAI GTCTC 2 cut(s) 632, 1036
BsnI GGCC 3 cut(s) 94, 593, 773
BsoBI CYCGRG 1 cut(s) 774
Bsp120I GGGCCC 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 162, 595
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 5 cut(s) 202, 262, 789, 808, 1180
Bsp1720I GCTNAGC 1 cut(s) 561
BspACI CCGC 1 cut(s) 623
BspANI GGCC 3 cut(s) 94, 593, 773
BspCNI CTCAG 7 cut(s) 66, 100, 217, 277, 440, 463, 553
BspLI GGNNCC 3 cut(s) 159, 593, 1127
BspMI ACCTGC 3 cut(s) 5, 913, 1078
BspPI GGATC 3 cut(s) 197, 797, 1175
BspQI GCTCTTC 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 825
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 4 cut(s) 508, 705, 970, 1048
BssECI CCNNGG 4 cut(s) 153, 360, 1077, 1166
BssMI GATC 5 cut(s) 202, 262, 789, 808, 1180
BssT1I CCWWGG 2 cut(s) 153, 1077
Bst2UI CCWGG 3 cut(s) 96, 362, 787
Bst4CI ACNGT 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 122
BstAUI TGTACA 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 1025
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 8 cut(s) 53, 75, 108, 225, 285, 427, 471, 561
BstDSI CCRYGG 1 cut(s) 1166
BstF5I GGATG 4 cut(s) 139, 153, 256, 679
BstKTI GATC 5 cut(s) 205, 265, 792, 811, 1183
BstMAI GTCTC 2 cut(s) 632, 1036
BstMBI GATC 5 cut(s) 202, 262, 789, 808, 1180
BstMWI GCNNNNNNNGC 5 cut(s) 122, 277, 770, 998, 1211
BstNI CCWGG 3 cut(s) 96, 362, 787
BstSCI CCNGG 4 cut(s) 94, 160, 360, 785
BstSLI GKGCMC 2 cut(s) 162, 595
BstV1I GCAGC 1 cut(s) 1004
BsuI GTATCC 2 cut(s) 284, 1138
BsuRI GGCC 3 cut(s) 94, 593, 773
BtgI CCRYGG 1 cut(s) 1166
BtsCI GGATG 4 cut(s) 139, 153, 256, 679
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 1149
BveI ACCTGC 3 cut(s) 5, 913, 1078
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 980
Cfr13I GGNCC 4 cut(s) 591, 592, 772, 1125
CseI GACGC 2 cut(s) 203, 359
Csp6I GTAC 1 cut(s) 446
CviAII CATG 3 cut(s) 859, 937, 1084
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 8 cut(s) 53, 75, 108, 225, 285, 427, 471, 561
DpnI GATC 5 cut(s) 204, 264, 791, 810, 1182
DpnII GATC 5 cut(s) 202, 262, 789, 808, 1180
EciI GGCGGA 1 cut(s) 638
Eco130I CCWWGG 2 cut(s) 153, 1077
Eco24I GRGCYC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 1125
Eco57I CTGAAG 2 cut(s) 383, 603
Eco72I CACGTG 1 cut(s) 1025
Eco88I CYCGRG 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 592
EcoRII CCWGG 3 cut(s) 94, 360, 785
EcoT14I CCWWGG 2 cut(s) 153, 1077
EcoT38I GRGCYC 1 cut(s) 595
ErhI CCWWGG 2 cut(s) 153, 1077
FaeI CATG 3 cut(s) 862, 940, 1087
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 512, 544
FatI CATG 3 cut(s) 858, 936, 1083
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 993
FokI GGATG 4 cut(s) 146, 160, 263, 686
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 1 cut(s) 993
FspBI CTAG 2 cut(s) 207, 606
GluI GCNGC 1 cut(s) 993
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 3 cut(s) 94, 593, 773
HapII CCGG 2 cut(s) 162, 277
HgaI GACGC 2 cut(s) 203, 359
Hin1II CATG 3 cut(s) 862, 940, 1087
HinfI GANTC 8 cut(s) 179, 467, 485, 602, 664, 682, 895, 1271
HpaII CCGG 2 cut(s) 162, 277
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 3 cut(s) 742, 1125, 1170
Hpy188III TCNNGA 4 cut(s) 107, 176, 245, 812
Hpy8I GTNNAC 3 cut(s) 742, 1125, 1170
Hpy99I CGWCG 2 cut(s) 131, 219
HpyAV CCTTC 9 cut(s) 320, 369, 431, 664, 682, 763, 820, 1107, 1255
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 1024
HpyCH4V TGCA 6 cut(s) 38, 908, 940, 992, 1087, 1235
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 277, 770, 998, 1211
HpyF3I CTNAG 8 cut(s) 53, 75, 108, 225, 285, 427, 471, 561
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 3 cut(s) 862, 940, 1087
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 5 cut(s) 202, 262, 789, 808, 1180
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 2 cut(s) 109, 406
Lsp1109I GCAGC 1 cut(s) 1004
LweI GCATC 5 cut(s) 112, 346, 504, 751, 967
MaeI CTAG 2 cut(s) 207, 606
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 1 cut(s) 7
MalI GATC 5 cut(s) 204, 264, 791, 810, 1182
MboI GATC 5 cut(s) 202, 262, 789, 808, 1180
MfeI CAATTG 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 162, 595
MluCI AATT 5 cut(s) 63, 300, 617, 1014, 1256
MlyI GAGTC 2 cut(s) 479, 1280
MmeI TCCRAC 2 cut(s) 312, 522
MroXI GAANNNNTTC 2 cut(s) 420, 681
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 957
MspI CCGG 2 cut(s) 162, 277
MspR9I CCNGG 4 cut(s) 96, 162, 362, 787
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 3 cut(s) 96, 362, 787
MwoI GCNNNNNNNGC 5 cut(s) 122, 277, 770, 998, 1211
NciI CCSGG 1 cut(s) 162
NdeII GATC 5 cut(s) 202, 262, 789, 808, 1180
NlaIII CATG 3 cut(s) 862, 940, 1087
NlaIV GGNNCC 3 cut(s) 159, 593, 1127
OliI CACNNNNGTG 1 cut(s) 957
PaqCI CACCTGC 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 489
PdmI GAANNNNTTC 2 cut(s) 420, 681
PfeI GAWTC 6 cut(s) 179, 467, 602, 664, 682, 895
PflFI GACNNNGTC 1 cut(s) 213
PflMI CCANNNNNTGG 1 cut(s) 1155
PkrI GCNGC 1 cut(s) 994
PleI GAGTC 2 cut(s) 479, 1279
PmaCI CACGTG 1 cut(s) 1025
PmlI CACGTG 1 cut(s) 1025
PpsI GAGTC 2 cut(s) 479, 1279
Ppu21I YACGTR 1 cut(s) 1025
Psp6I CCWGG 3 cut(s) 94, 360, 785
PspCI CACGTG 1 cut(s) 1025
PspGI CCWGG 3 cut(s) 94, 360, 785
PspN4I GGNNCC 3 cut(s) 159, 593, 1127
PspOMI GGGCCC 1 cut(s) 591
PspPI GGNCC 4 cut(s) 591, 592, 772, 1125
PstNI CAGNNNCTG 1 cut(s) 980
PsyI GACNNNGTC 1 cut(s) 213
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 957
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 1 cut(s) 993
Sau3AI GATC 5 cut(s) 202, 262, 789, 808, 1180
Sau96I GGNCC 4 cut(s) 591, 592, 772, 1125
SchI GAGTC 2 cut(s) 479, 1280
ScrFI CCNGG 4 cut(s) 96, 162, 362, 787
SduI GDGCHC 2 cut(s) 162, 595
SfaNI GCATC 5 cut(s) 112, 346, 504, 751, 967
SinI GGWCC 1 cut(s) 1125
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 2 cut(s) 575, 596
SmoI CTYRAG 2 cut(s) 575, 596
Sse9I AATT 5 cut(s) 63, 300, 617, 1014, 1256
SsiI CCGC 1 cut(s) 623
SspI AATATT 3 cut(s) 712, 947, 1071
SspMI CTAG 2 cut(s) 207, 606
StyD4I CCNGG 4 cut(s) 94, 160, 360, 785
StyI CCWWGG 2 cut(s) 153, 1077
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 1027
TasI AATT 5 cut(s) 63, 300, 617, 1014, 1256
TatI WGTACW 1 cut(s) 445
TfiI GAWTC 6 cut(s) 179, 467, 602, 664, 682, 895
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 2 cut(s) 45, 1156
TseI GCWGC 1 cut(s) 992
TspDTI ATGAA 4 cut(s) 690, 750, 925, 1235
TspGWI ACGGA 2 cut(s) 643, 1155
TspRI CASTG 2 cut(s) 45, 1156
Tth111I GACNNNGTC 1 cut(s) 213
Van91I CCANNNNNTGG 1 cut(s) 1155
VpaK11BI GGWCC 1 cut(s) 1125
XapI RAATTY 1 cut(s) 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 924
XmnI GAANNNNTTC 2 cut(s) 420, 681
XspI CTAG 2 cut(s) 207, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.