RchiOBHm_Chr7g0222731

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002954
Physical Location & Seq
Reverse (-)
44390016 .. 44394232
4217 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19938

Sequence Viewer

Length: 1308 bp
ATGTCGGTCACTGCAGGTGTTAGTGACACTGTGATTGCCATTAGGGATAAGCTGAGAGGTAAAATTGGACAGACTAAAGTTAAAAGGTATTGGCCTGGAAAAGCTCCTGAGTGGGGAGATGATGCCGACGAAGATGGCGATATTAGGTTGTCCGCGGCTGCCTCCCTCGAGAAAGCTTTCCCCACTCAGGATTATTCCGATATTGCAAGGAAAGATGATCCTAGGCTGCGTCGTTTGGCTGAGAGCAGGATAGATAATCGCGACGATGTTAGGGCTGATCACAGGCGTATCCGCCAAGCTGAAATTGTTTCAACTATTGAAGAGGAAGCCAGGAGGCAGGAAGGGATAGATGCCGAAGAAGAGGATCCTGATGCTTTAGAGGAAAGGAGAAGAAGGATTAAGGAAAGGTTGCGTGAGAGGGAGCAAGAAGAAGCTGCACTCCTCCCCTCCGAAGATGAGGAAGAGGATAAGGAAGAAGAGGAGGAGGAAGAGGAATCAGAGTATTCAACTGACTCAGAAGATGAGCATGGTGGTATGATGATGCTCAAGCCAGTCTTTGTTCCCAAGGCTGAGAGAGATACCATCGCTGAACGTGAGCGAATTGAGGCAGAAGAACTGGCCCTTGAGGAAGCAAGGAAGAAAAAATTGGAGGAGAGGAAGAGAGAAACAAAGCAGATTGTGGTTGAGGAAATCCGAAAGGATGAAGAGATACAGAAAAGTATGGAACAGGATGCTAACATTGCTGATATTGATACTGATGATGAACTTAACGAGGCAGAGGAGTATGAAGCTTGGAAGGCAAGAGAGATTGCCAGGATCAAGAGGGATAGGGAGGAAAGAGAAGCGATGTTGAAGGAAAAGGAAGAGATTGAAAGAGTAAGAAATATGACAGAGGAAGAGAGGAGGGAATGGGAGAGGAAGAATCCTAAACCTGCACCAAAACCAAAGCAAAAGTGGAGGTTCATGCAGAAGTACCACCACAAAGGTGCTTTCTTCCAGTCAGACGCAGATGACCATGCTGCAACTGTGGGAACAGATGGAATTTTTACTCGTGATTTCTCTGCTCCAACTGGAGAAGATAAAATGGACAAAACTATATTGCCGAAGGTCATGCAAGTCAAGCATTTTGGTCGAAGTGGAAGGACAAAATGGACCCATCTTGTCAATGAGGATACTACTGACTGGAACAATCCGTGGACCTACAATGATCCTCTTCGGGCAAAGTATAATGCTAAAATGGCCGGAATGAATGCACCTATAGAAAAACCCAGAGGAAGCAAGAAATTGAAGGATCGGGAGTATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

435

Amino Acids

51.2

Weight (kDa)

5.03

Isoelectric Point (pI)

59.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 171 - 392 6.2e-77 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
Acc36I ACCTGC 2 cut(s) 5, 940
AccII CGCG 2 cut(s) 155, 261
AciI CCGC 3 cut(s) 153, 155, 292
AclWI GGATC 6 cut(s) 212, 359, 372, 824, 1202, 1299
AcoI YGGCCR 1 cut(s) 1239
AcsI RAATTY 1 cut(s) 1041
AfaI GTAC 1 cut(s) 974
AfiI CCNNNNNNNGG 2 cut(s) 113, 187
AgsI TTSAA 6 cut(s) 312, 320, 507, 853, 872, 1288
AjnI CCWGG 3 cut(s) 94, 329, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 984
AluBI AGCT 6 cut(s) 52, 104, 176, 299, 434, 791
AluI AGCT 6 cut(s) 52, 104, 176, 299, 434, 791
AlwI GGATC 6 cut(s) 212, 359, 372, 824, 1202, 1299
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 3 cut(s) 92, 618, 1239
ApeKI GCWGC 4 cut(s) 158, 226, 434, 1019
ApoI RAATTY 1 cut(s) 1041
Asp700I GAANNNNTTC 1 cut(s) 176
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 619, 1152, 1197
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 1152, 1197
AvrII CCTAGG 1 cut(s) 221
BaeI ACNNNNGTAYC 2 cut(s) 271, 304
BamHI GGATCC 1 cut(s) 364
BauI CACGAG 1 cut(s) 1050
BbvI GCAGC 4 cut(s) 145, 213, 421, 1006
BccI CCATC 4 cut(s) 128, 590, 1031, 1164
BcgI CGANNNNNNTGC 4 cut(s) 1093, 1112, 1127, 1146
BciT130I CCWGG 3 cut(s) 96, 331, 814
BciVI GTATCC 2 cut(s) 299, 1165
BclI TGATCA 1 cut(s) 277
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 2 cut(s) 12, 1257
BfuAI ACCTGC 2 cut(s) 5, 940
BfuI GTATCC 2 cut(s) 299, 1165
BisI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
BlnI CCTAGG 1 cut(s) 221
BlsI GCNGC 5 cut(s) 157, 160, 228, 436, 1021
Bme1390I CCNGG 3 cut(s) 96, 331, 814
Bme18I GGWCC 2 cut(s) 1152, 1197
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 3 cut(s) 619, 1152, 1197
BmiI GGNNCC 2 cut(s) 366, 1154
BmrFI CCNGG 3 cut(s) 96, 331, 814
BmsI GCATC 5 cut(s) 112, 340, 361, 531, 721
BpmI CTGGAG 1 cut(s) 1092
BpuEI CTTGAG 2 cut(s) 530, 644
BsaJI CCNNGG 4 cut(s) 153, 221, 564, 1193
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 187
Bse1I ACTGG 5 cut(s) 551, 621, 997, 1075, 1187
Bse3DI GCAATG 1 cut(s) 738
BseBI CCWGG 3 cut(s) 96, 331, 814
BseDI CCNNGG 4 cut(s) 153, 221, 564, 1193
BseGI GGATG 2 cut(s) 706, 736
BseLI CCNNNNNNNGG 2 cut(s) 113, 187
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 6 cut(s) 44, 99, 200, 231, 528, 561
BseNI ACTGG 5 cut(s) 551, 621, 997, 1075, 1187
BseRI GAGGAG 6 cut(s) 431, 494, 497, 665, 794, 916
BseXI GCAGC 4 cut(s) 145, 213, 421, 1006
BsgI GTGCAG 2 cut(s) 420, 918
Bsh1236I CGCG 2 cut(s) 155, 261
BshFI GGCC 3 cut(s) 94, 620, 1241
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 1 cut(s) 1242
BslI CCNNNNNNNGG 2 cut(s) 113, 187
BsmI GAATGC 1 cut(s) 1255
BsnI GGCC 3 cut(s) 94, 620, 1241
BsoBI CYCGRG 1 cut(s) 167
Bsp143I GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 3 cut(s) 153, 155, 292
BspANI GGCC 3 cut(s) 94, 620, 1241
BspCNI CTCAG 6 cut(s) 45, 100, 199, 232, 527, 562
BspFNI CGCG 2 cut(s) 155, 261
BspLI GGNNCC 2 cut(s) 366, 1154
BspMAI CTGCAG 1 cut(s) 16
BspMI ACCTGC 2 cut(s) 5, 940
BspPI GGATC 6 cut(s) 212, 359, 372, 824, 1202, 1299
BsrDI GCAATG 1 cut(s) 738
BsrI ACTGG 5 cut(s) 551, 621, 997, 1075, 1187
BssECI CCNNGG 4 cut(s) 153, 221, 564, 1193
BssMI GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
BssSI CACGAG 1 cut(s) 1050
BssT1I CCWWGG 2 cut(s) 221, 564
Bst2BI CACGAG 1 cut(s) 1050
Bst2UI CCWGG 3 cut(s) 96, 331, 814
Bst4CI ACNGT 2 cut(s) 31, 1027
BstDEI CTNAG 6 cut(s) 53, 108, 186, 240, 514, 570
BstDSI CCRYGG 2 cut(s) 153, 1193
BstF5I GGATG 2 cut(s) 706, 736
BstFNI CGCG 2 cut(s) 155, 261
BstKTI GATC 6 cut(s) 220, 280, 367, 819, 1210, 1294
BstMBI GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
BstMWI GCNNNNNNNGC 4 cut(s) 740, 797, 1120, 1238
BstNI CCWGG 3 cut(s) 96, 331, 814
BstSCI CCNGG 3 cut(s) 94, 329, 812
BstSFI CTRYAG 2 cut(s) 12, 1257
BstUI CGCG 2 cut(s) 155, 261
BstV1I GCAGC 4 cut(s) 145, 213, 421, 1006
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 2 cut(s) 299, 1165
BsuRI GGCC 3 cut(s) 94, 620, 1241
BtgI CCRYGG 2 cut(s) 153, 1193
BtgZI GCGATG 2 cut(s) 568, 860
BtsCI GGATG 2 cut(s) 706, 736
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 9, 27
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 2 cut(s) 5, 940
Cfr13I GGNCC 3 cut(s) 619, 1152, 1197
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 2 cut(s) 218, 1013
Csp6I GTAC 1 cut(s) 973
CviAII CATG 4 cut(s) 527, 964, 1016, 1111
CviQI GTAC 1 cut(s) 973
DdeI CTNAG 6 cut(s) 53, 108, 186, 240, 514, 570
DpnI GATC 6 cut(s) 219, 279, 366, 818, 1209, 1293
DpnII GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
EaeI YGGCCR 1 cut(s) 1239
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 221, 564
Eco47I GGWCC 2 cut(s) 1152, 1197
Eco88I CYCGRG 1 cut(s) 167
EcoRII CCWGG 3 cut(s) 94, 329, 812
EcoT14I CCWWGG 2 cut(s) 221, 564
ErhI CCWWGG 2 cut(s) 221, 564
FaeI CATG 4 cut(s) 530, 967, 1019, 1114
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FatI CATG 4 cut(s) 526, 963, 1015, 1110
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
FokI GGATG 2 cut(s) 713, 743
Fsp4HI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
FspBI CTAG 1 cut(s) 222
GluI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
GsuI CTGGAG 1 cut(s) 1092
HaeIII GGCC 3 cut(s) 94, 620, 1241
HapII CCGG 1 cut(s) 1242
HgaI GACGC 2 cut(s) 218, 1013
Hin1II CATG 4 cut(s) 530, 967, 1019, 1114
HindIII AAGCTT 2 cut(s) 174, 789
HinfI GANTC 3 cut(s) 494, 512, 922
HpaII CCGG 1 cut(s) 1242
Hpy166II GTNNAC 1 cut(s) 1197
Hpy188I TCNGA 6 cut(s) 199, 451, 499, 517, 695, 1003
Hpy188III TCNNGA 8 cut(s) 107, 169, 188, 260, 368, 820, 1052, 1295
Hpy8I GTNNAC 1 cut(s) 1197
Hpy99I CGWCG 3 cut(s) 131, 234, 266
HpyAV CCTTC 7 cut(s) 335, 387, 790, 847, 1099, 1134, 1282
HpyCH4III ACNGT 2 cut(s) 31, 1027
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 8 cut(s) 14, 206, 437, 935, 967, 1022, 1114, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 740, 797, 1120, 1238
HpyF3I CTNAG 6 cut(s) 53, 108, 186, 240, 514, 570
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 4 cut(s) 530, 967, 1019, 1114
Ksp22I TGATCA 1 cut(s) 277
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
LmnI GCTCC 3 cut(s) 109, 421, 1069
Lsp1109I GCAGC 4 cut(s) 145, 213, 421, 1006
LweI GCATC 5 cut(s) 112, 340, 361, 531, 721
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 2 cut(s) 7, 23
MalI GATC 6 cut(s) 219, 279, 366, 818, 1209, 1293
MboI GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
MflI RGATCY 1 cut(s) 364
MluCI AATT 6 cut(s) 63, 303, 600, 644, 1041, 1283
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 1091
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 3 cut(s) 81, 399, 768
MslI CAYNNNNRTG 1 cut(s) 984
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 1242
MspR9I CCNGG 3 cut(s) 96, 331, 814
Mva1269I GAATGC 1 cut(s) 1255
MvaI CCWGG 3 cut(s) 96, 331, 814
MvnI CGCG 2 cut(s) 155, 261
MwoI GCNNNNNNNGC 4 cut(s) 740, 797, 1120, 1238
NdeII GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
NlaIII CATG 4 cut(s) 530, 967, 1019, 1114
NlaIV GGNNCC 2 cut(s) 366, 1154
NmuCI GTSAC 2 cut(s) 7, 23
NruI TCGCGA 1 cut(s) 261
OliI CACNNNNGTG 1 cut(s) 984
PaeR7I CTCGAG 1 cut(s) 167
PaqCI CACCTGC 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 1255
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 494, 922
PkrI GCNGC 5 cut(s) 157, 160, 228, 436, 1021
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 3 cut(s) 94, 329, 812
PspGI CCWGG 3 cut(s) 94, 329, 812
PspN4I GGNNCC 2 cut(s) 366, 1154
PspPI GGNCC 3 cut(s) 619, 1152, 1197
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 364
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 1 cut(s) 974
RsaNI GTAC 1 cut(s) 973
RseI CAYNNNNRTG 1 cut(s) 984
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 3 cut(s) 81, 399, 768
SatI GCNGC 5 cut(s) 156, 159, 227, 435, 1020
Sau3AI GATC 6 cut(s) 217, 277, 364, 816, 1207, 1291
Sau96I GGNCC 3 cut(s) 619, 1152, 1197
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 3 cut(s) 96, 331, 814
SfaNI GCATC 5 cut(s) 112, 340, 361, 531, 721
SfcI CTRYAG 2 cut(s) 12, 1257
Sfr274I CTCGAG 1 cut(s) 167
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 2 cut(s) 1152, 1197
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 3 cut(s) 167, 545, 623
SmoI CTYRAG 3 cut(s) 167, 545, 623
Sse9I AATT 6 cut(s) 63, 303, 600, 644, 1041, 1283
SsiI CCGC 3 cut(s) 153, 155, 292
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 94, 329, 812
StyI CCWWGG 2 cut(s) 221, 564
TaaI ACNGT 2 cut(s) 31, 1027
TaiI ACGT 1 cut(s) 595
TaqI TCGA 2 cut(s) 168, 1132
TasI AATT 6 cut(s) 63, 303, 600, 644, 1041, 1283
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 494, 922
Tru1I TTAA 3 cut(s) 81, 399, 768
Tru9I TTAA 3 cut(s) 81, 399, 768
TscAI CASTG 2 cut(s) 16, 34
TseFI GTSAC 2 cut(s) 7, 23
TseI GCWGC 4 cut(s) 158, 226, 434, 1019
Tsp45I GTSAC 2 cut(s) 7, 23
TspDTI ATGAA 5 cut(s) 717, 777, 801, 952, 1262
TspGWI ACGGA 1 cut(s) 1182
TspRI CASTG 2 cut(s) 16, 34
VpaK11BI GGWCC 2 cut(s) 1152, 1197
XapI RAATTY 1 cut(s) 1041
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XhoI CTCGAG 1 cut(s) 167
XmaJI CCTAGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.