Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0002630

Physical Location & Seq

Genomic Coordinates Forward (+)

23432417 .. 23437997

Gene Structure 5581 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g32470.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGGCGAAGACCCAAGTCTCAGACCACCTGGAGCCATGGCACGACCTCCCCGACAAGGTCGTCATGGTCACCGGCGCCTCTTCCGGCCTCGGCCATGACTTCTGTCTCGACTTGGCTAAAGCCGGCTGCCGGATTGTCGCCGCCGCACGCCGCGTCGATCGCCTCCACTCTATCTGTGACGCAATCAACAAACTTTCCACCACCGCGCCGCCTCAGACGAGGGCCTTCGCGGTTGAGCTCGACGTCAGCGCCGACGGCTCTGCTATTCAGAAGTCTGTGCAAAAGGCTTGGGATGCCTTTGGTCGCATCGACGCCCTCATCAACAACGCCGGTGTTAGAGGTACTGTGGCATCTGCATTGGAATTGTCTGAGGAGGAATGGAACAACACGTTAAAGACAAACTTGACAGGCACCTGGTTGGTATCAAAATATGTTTCCCTATGCATGCGTAATGCAGATAAGGGAGGATCAATCATTAATATATCTTCCATTGCTGGTCTTAATCGTGGATATTTGCCTGGAGGTCCAGCATACAATTGCTCAAAGGGTGCCGTAAACACCTTGTCCAAGGTCATGGCTTTGGAGTTGGGGGCTCACAAGATCAGAGTGAATGCAATATCACCTGGGCTGTTCAGATCTGAAATTACAGAAGGTCTTATGCAAAAAGATTGGCTACACAATGTGGCTATGAGAACTGTCCCTTTGAGAACATTTGGCACTTCAGATCCAGCATTGACATCACTAGTTCGTTATTTGATACATGACTCCTCTGAGTATGTCTCGGGAAATATCTACATTGTAGACGCAGGAGCCACTTTGCCAGGGAGGTTTGGGGTTGTGGATGTTGCCCATCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004312 GO:0004316 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0030497 GO:0032787 GO:0036094 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0048037 GO:0050661 GO:0050662 GO:0055114 GO:0071704 GO:0072330 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

adh_short adh_short_C2

Protein Analysis

286

Amino Acids

30.55

Weight (kDa)

7.71

Isoelectric Point (pI)

29.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	19 - 221	4.4e-51	short chain dehydrogenase
KR	PF08659	21 - 168	1.1e-08	KR domain
adh_short_C2	PF13561	25 - 272	2.8e-45	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62610 AT1G62610 AT1G62610 AT1G62610 AT1G63380 AT1G63380 AT1G63380 AT1G63380 AT2G17845 AT3G46170 AT3G55290 AT3G55290 AT3G55310
fragaria_vesca	FvH4_5g32470
malus_domestica	MD12G1006600.v1.1 MD12G1094700.v1.1 MD15G1420800.v1.1 MD15G1420900.v1.1
prunus_persica	Prupe.7G031300_v2.0.a1 Prupe.7G031300_v2.0.a1
pyrus_communis	pycom13g09640 pycom14g08370
rosa_chinensis	RchiOBHm_Chr7g0229021 RchiOBHm_Chr7g0229061
rosa_laevigata	RLG00000001534
rosa_multiflora	Rmu_sc0000271.1_g000029 Rmu_sc0000271.1_g000037 Rmu_sc0009986.1_g000002
rosa_roxburghii	Rroxscaffold_3G00230780
rosa_rugosa	Rorug07G0254900 Rorug07G0255400
rosa_samantha	Rh7AG406300 Rh7AG406400 Rh7BG385600 Rh7BG385700 Rh7CG423900 Rh7CG424100 Rh7DG400700
rosa_wichuraiana	Rw7G033710 Rw7G033730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	59
AatII	GACGTC	1 cut(s)	246
AccB1I	GGYRCC	3 cut(s)	74, 410, 548
AccI	GTMKAC	1 cut(s)	801
AccII	CGCG	3 cut(s)	153, 206, 230
AciI	CCGC	6 cut(s)	141, 144, 151, 204, 209, 230
AclWI	GGATC	2 cut(s)	475, 719
AcoI	YGGCCR	1 cut(s)	91
AcuI	CTGAAG	1 cut(s)	705
AcyI	GRCGYC	3 cut(s)	75, 243, 312
AdeI	CACNNNGTG	1 cut(s)	682
AfaI	GTAC	1 cut(s)	343
AfiI	CCNNNNNNNGG	2 cut(s)	55, 129
AflIII	ACRYGT	1 cut(s)	387
AhlI	ACTAGT	1 cut(s)	742
AjnI	CCWGG	5 cut(s)	27, 413, 517, 622, 820
AluBI	AGCT	1 cut(s)	238
AluI	AGCT	1 cut(s)	238
Alw21I	GWGCWC	1 cut(s)	240
Alw26I	GTCTC	3 cut(s)	22, 110, 784
AlwI	GGATC	2 cut(s)	475, 719
Ama87I	CYCGRG	1 cut(s)	781
AoxI	GGCC	3 cut(s)	85, 91, 222
ApeKI	GCWGC	1 cut(s)	126
AseI	ATTAAT	1 cut(s)	477
AspLEI	GCGC	3 cut(s)	77, 208, 251
AspS9I	GGNCC	2 cut(s)	222, 524
AsuHPI	GGTGA	2 cut(s)	61, 612
AvaI	CYCGRG	1 cut(s)	781
AvaII	GGWCC	1 cut(s)	524
BanI	GGYRCC	3 cut(s)	74, 410, 548
BanII	GRGCYC	2 cut(s)	240, 595
BbsI	GAAGAC	1 cut(s)	14
Bbv12I	GWGCWC	1 cut(s)	240
BbvI	GCAGC	1 cut(s)	113
BceAI	ACGGC	2 cut(s)	271, 536
BcgI	CGANNNNNNTGC	2 cut(s)	242, 276
BciT130I	CCWGG	5 cut(s)	29, 415, 519, 624, 822
BcoDI	GTCTC	3 cut(s)	22, 110, 784
BcuI	ACTAGT	1 cut(s)	742
BfaI	CTAG	1 cut(s)	743
BfoI	RGCGCY	2 cut(s)	78, 252
BglII	AGATCT	1 cut(s)	635
BisI	GCNGC	5 cut(s)	127, 141, 144, 151, 209
BlsI	GCNGC	5 cut(s)	128, 142, 145, 152, 210
Bme1390I	CCNGG	5 cut(s)	29, 415, 519, 624, 822
Bme18I	GGWCC	1 cut(s)	524
BmeT110I	CYCGRG	1 cut(s)	781
BmgT120I	GGNCC	2 cut(s)	222, 524
BmiI	GGNNCC	5 cut(s)	33, 76, 412, 550, 811
BmrFI	CCNGG	5 cut(s)	29, 415, 519, 624, 822
BmsI	GCATC	3 cut(s)	283, 315, 359
BoxI	GACNNNNGTC	2 cut(s)	14, 102
BpiI	GAAGAC	1 cut(s)	14
BplI	GAGNNNNNCTC	2 cut(s)	764, 796
BpmI	CTGGAG	2 cut(s)	50, 540
BsaHI	GRCGYC	3 cut(s)	75, 243, 312
BsaJI	CCNNGG	5 cut(s)	35, 88, 567, 623, 821
Bsc4I	CCNNNNNNNGG	2 cut(s)	55, 129
Bse118I	RCCGGY	3 cut(s)	71, 122, 329
Bse3DI	GCAATG	1 cut(s)	489
BseBI	CCWGG	5 cut(s)	29, 415, 519, 624, 822
BseDI	CCNNGG	5 cut(s)	35, 88, 567, 623, 821
BseGI	GGATG	2 cut(s)	298, 847
BseLI	CCNNNNNNNGG	2 cut(s)	55, 129
BseMI	GCAATG	1 cut(s)	489
BseMII	CTCAG	4 cut(s)	33, 227, 360, 762
BseRI	GAGGAG	2 cut(s)	386, 757
BseXI	GCAGC	1 cut(s)	113
Bsh1236I	CGCG	3 cut(s)	153, 206, 230
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	3 cut(s)	87, 93, 224
BshNI	GGYRCC	3 cut(s)	74, 410, 548
BsiEI	CGRYCG	1 cut(s)	160
BsiHKAI	GWGCWC	1 cut(s)	240
BsiHKCI	CYCGRG	1 cut(s)	781
BsiSI	CCGG	5 cut(s)	72, 84, 123, 130, 330
BslFI	GGGAC	1 cut(s)	683
BslI	CCNNNNNNNGG	2 cut(s)	55, 129
BsmAI	GTCTC	3 cut(s)	22, 110, 784
BsmFI	GGGAC	1 cut(s)	683
BsmI	GAATGC	1 cut(s)	616
BsnI	GGCC	3 cut(s)	87, 93, 224
BsoBI	CYCGRG	1 cut(s)	781
Bsp1286I	GDGCHC	2 cut(s)	240, 595
Bsp143I	GATC	5 cut(s)	157, 467, 600, 635, 724
Bsp19I	CCATGG	1 cut(s)	35
BspACI	CCGC	6 cut(s)	141, 144, 151, 204, 209, 230
BspANI	GGCC	3 cut(s)	87, 93, 224
BspCNI	CTCAG	4 cut(s)	32, 226, 361, 763
BspFNI	CGCG	3 cut(s)	153, 206, 230
BspLI	GGNNCC	5 cut(s)	33, 76, 412, 550, 811
BspPI	GGATC	2 cut(s)	475, 719
BspT107I	GGYRCC	3 cut(s)	74, 410, 548
BsrDI	GCAATG	1 cut(s)	489
BsrFI	RCCGGY	3 cut(s)	71, 122, 329
BssAI	RCCGGY	3 cut(s)	71, 122, 329
BssECI	CCNNGG	5 cut(s)	35, 88, 567, 623, 821
BssMI	GATC	5 cut(s)	157, 467, 600, 635, 724
BssNI	GRCGYC	3 cut(s)	75, 243, 312
BssT1I	CCWWGG	2 cut(s)	35, 567
Bst2UI	CCWGG	5 cut(s)	29, 415, 519, 624, 822
Bst4CI	ACNGT	2 cut(s)	346, 697
Bst6I	CTCTTC	1 cut(s)	85
BstACI	GRCGYC	3 cut(s)	75, 243, 312
BstC8I	GCNNGC	3 cut(s)	124, 148, 446
BstDEI	CTNAG	4 cut(s)	19, 213, 369, 771
BstDSI	CCRYGG	1 cut(s)	35
BstEII	GGTNACC	1 cut(s)	67
BstF5I	GGATG	2 cut(s)	298, 847
BstFNI	CGCG	3 cut(s)	153, 206, 230
BstH2I	RGCGCY	2 cut(s)	78, 252
BstHHI	GCGC	3 cut(s)	77, 208, 251
BstKTI	GATC	5 cut(s)	160, 470, 603, 638, 727
BstMAI	GTCTC	3 cut(s)	22, 110, 784
BstMBI	GATC	5 cut(s)	157, 467, 600, 635, 724
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	3 cut(s)	159, 255, 293
BstNI	CCWGG	5 cut(s)	29, 415, 519, 624, 822
BstNSI	RCATGY	1 cut(s)	448
BstPAI	GACNNNNGTC	2 cut(s)	14, 102
BstPI	GGTNACC	1 cut(s)	67
BstSCI	CCNGG	5 cut(s)	27, 413, 517, 622, 820
BstUI	CGCG	3 cut(s)	153, 206, 230
BstV1I	GCAGC	1 cut(s)	113
BstV2I	GAAGAC	1 cut(s)	14
BstX2I	RGATCY	2 cut(s)	635, 724
BstXI	CCANNNNNNTGG	1 cut(s)	574
BstYI	RGATCY	2 cut(s)	635, 724
BsuRI	GGCC	3 cut(s)	87, 93, 224
BtgI	CCRYGG	1 cut(s)	35
BtsCI	GGATG	2 cut(s)	298, 847
BtsIMutI	CAGTG	1 cut(s)	853
Cac8I	GCNNGC	3 cut(s)	124, 148, 446
CfoI	GCGC	3 cut(s)	77, 208, 251
Cfr10I	RCCGGY	3 cut(s)	71, 122, 329
Cfr13I	GGNCC	2 cut(s)	222, 524
CseI	GACGC	4 cut(s)	142, 188, 320, 812
CsiI	ACCWGGT	1 cut(s)	413
Csp6I	GTAC	1 cut(s)	342
CviAII	CATG	6 cut(s)	36, 64, 95, 445, 574, 761
CviQI	GTAC	1 cut(s)	342
DdeI	CTNAG	4 cut(s)	19, 213, 369, 771
DinI	GGCGCC	1 cut(s)	76
DpnI	GATC	5 cut(s)	159, 469, 602, 637, 726
DpnII	GATC	5 cut(s)	157, 467, 600, 635, 724
DraIII	CACNNNGTG	1 cut(s)	682
DrdI	GACNNNNNNGTC	1 cut(s)	59
DseDI	GACNNNNNNGTC	1 cut(s)	59
EaeI	YGGCCR	1 cut(s)	91
Eam1104I	CTCTTC	1 cut(s)	85
EarI	CTCTTC	1 cut(s)	85
Ecl136II	GAGCTC	1 cut(s)	238
Eco130I	CCWWGG	2 cut(s)	35, 567
Eco24I	GRGCYC	2 cut(s)	240, 595
Eco47I	GGWCC	1 cut(s)	524
Eco53kI	GAGCTC	1 cut(s)	238
Eco57I	CTGAAG	1 cut(s)	705
Eco88I	CYCGRG	1 cut(s)	781
Eco91I	GGTNACC	1 cut(s)	67
EcoICRI	GAGCTC	1 cut(s)	238
EcoO109I	RGGNCCY	1 cut(s)	222
EcoO65I	GGTNACC	1 cut(s)	67
EcoRII	CCWGG	5 cut(s)	27, 413, 517, 622, 820
EcoT14I	CCWWGG	2 cut(s)	35, 567
EcoT22I	ATGCAT	1 cut(s)	446
EcoT38I	GRGCYC	2 cut(s)	240, 595
EgeI	GGCGCC	1 cut(s)	76
EheI	GGCGCC	1 cut(s)	76
ErhI	CCWWGG	2 cut(s)	35, 567
FaeI	CATG	6 cut(s)	39, 67, 98, 448, 577, 764
FalI	AAGNNNNNCTT	2 cut(s)	386, 418
FaqI	GGGAC	1 cut(s)	683
FatI	CATG	6 cut(s)	35, 63, 94, 444, 573, 760
FblI	GTMKAC	1 cut(s)	801
Fnu4HI	GCNGC	5 cut(s)	127, 141, 144, 151, 209
FokI	GGATG	2 cut(s)	305, 854
FriOI	GRGCYC	2 cut(s)	240, 595
Fsp4HI	GCNGC	5 cut(s)	127, 141, 144, 151, 209
FspBI	CTAG	1 cut(s)	743
GlaI	GCGC	3 cut(s)	76, 207, 250
GluI	GCNGC	5 cut(s)	127, 141, 144, 151, 209
GsuI	CTGGAG	2 cut(s)	50, 540
HaeII	RGCGCY	2 cut(s)	78, 252
HaeIII	GGCC	3 cut(s)	87, 93, 224
HapII	CCGG	5 cut(s)	72, 84, 123, 130, 330
HgaI	GACGC	4 cut(s)	142, 188, 320, 812
HhaI	GCGC	3 cut(s)	77, 208, 251
Hin1I	GRCGYC	3 cut(s)	75, 243, 312
Hin1II	CATG	6 cut(s)	39, 67, 98, 448, 577, 764
Hin6I	GCGC	3 cut(s)	75, 206, 249
HinP1I	GCGC	3 cut(s)	75, 206, 249
HinfI	GANTC	1 cut(s)	764
HpaII	CCGG	5 cut(s)	72, 84, 123, 130, 330
HphI	GGTGA	2 cut(s)	61, 612
Hpy166II	GTNNAC	2 cut(s)	556, 802
Hpy188I	TCNGA	9 cut(s)	22, 216, 270, 370, 605, 635, 640, 724, 772
Hpy188III	TCNNGA	2 cut(s)	107, 783
Hpy8I	GTNNAC	2 cut(s)	556, 802
Hpy99I	CGWCG	4 cut(s)	158, 245, 257, 314
HpyAV	CCTTC	2 cut(s)	235, 644
HpyCH4III	ACNGT	2 cut(s)	346, 697
HpyCH4IV	ACGT	2 cut(s)	243, 389
HpyCH4V	TGCA	6 cut(s)	280, 356, 444, 455, 614, 661
HpyF10VI	GCNNNNNNNGC	3 cut(s)	159, 255, 293
HpyF3I	CTNAG	4 cut(s)	19, 213, 369, 771
HpySE526I	ACGT	2 cut(s)	243, 389
Hsp92I	GRCGYC	3 cut(s)	75, 243, 312
Hsp92II	CATG	6 cut(s)	39, 67, 98, 448, 577, 764
HspAI	GCGC	3 cut(s)	75, 206, 249
KasI	GGCGCC	1 cut(s)	74
KroI	GCCGGC	1 cut(s)	122
KroNI	GCCGGC	1 cut(s)	124
Kzo9I	GATC	5 cut(s)	157, 467, 600, 635, 724
LmnI	GCTCC	2 cut(s)	31, 809
Lsp1109I	GCAGC	1 cut(s)	113
LweI	GCATC	3 cut(s)	283, 315, 359
MabI	ACCWGGT	1 cut(s)	413
MaeI	CTAG	1 cut(s)	743
MaeII	ACGT	2 cut(s)	243, 389
MaeIII	GTNAC	2 cut(s)	67, 176
MalI	GATC	5 cut(s)	159, 469, 602, 637, 726
MboI	GATC	5 cut(s)	157, 467, 600, 635, 724
MboII	GAAGA	3 cut(s)	19, 72, 477
MfeI	CAATTG	1 cut(s)	535
MflI	RGATCY	2 cut(s)	635, 724
MhlI	GDGCHC	2 cut(s)	240, 595
MluCI	AATT	3 cut(s)	362, 535, 642
Mly113I	GGCGCC	1 cut(s)	75
MlyI	GAGTC	1 cut(s)	758
Mph1103I	ATGCAT	1 cut(s)	446
MroNI	GCCGGC	1 cut(s)	122
MseI	TTAA	3 cut(s)	392, 477, 501
MspI	CCGG	5 cut(s)	72, 84, 123, 130, 330
MspR9I	CCNGG	5 cut(s)	29, 415, 519, 624, 822
MunI	CAATTG	1 cut(s)	535
Mva1269I	GAATGC	1 cut(s)	616
MvaI	CCWGG	5 cut(s)	29, 415, 519, 624, 822
MvnI	CGCG	3 cut(s)	153, 206, 230
MwoI	GCNNNNNNNGC	3 cut(s)	159, 255, 293
NaeI	GCCGGC	1 cut(s)	124
NarI	GGCGCC	1 cut(s)	75
NcoI	CCATGG	1 cut(s)	35
NdeII	GATC	5 cut(s)	157, 467, 600, 635, 724
NgoMIV	GCCGGC	1 cut(s)	122
NlaIII	CATG	6 cut(s)	39, 67, 98, 448, 577, 764
NlaIV	GGNNCC	5 cut(s)	33, 76, 412, 550, 811
NmeAIII	GCCGAG	1 cut(s)	69
NmuCI	GTSAC	2 cut(s)	67, 176
NsiI	ATGCAT	1 cut(s)	446
NspI	RCATGY	1 cut(s)	448
PaeI	GCATGC	1 cut(s)	448
PcsI	WCGNNNNNNNCGW	2 cut(s)	48, 249
PctI	GAATGC	1 cut(s)	616
PdiI	GCCGGC	1 cut(s)	124
PflFI	GACNNNGTC	1 cut(s)	56
PkrI	GCNGC	5 cut(s)	128, 142, 145, 152, 210
Ple19I	CGATCG	1 cut(s)	160
PleI	GAGTC	1 cut(s)	758
PluTI	GGCGCC	1 cut(s)	78
PpsI	GAGTC	1 cut(s)	758
PshAI	GACNNNNGTC	2 cut(s)	14, 102
PshBI	ATTAAT	1 cut(s)	477
Psp124BI	GAGCTC	1 cut(s)	240
Psp6I	CCWGG	5 cut(s)	27, 413, 517, 622, 820
PspEI	GGTNACC	1 cut(s)	67
PspGI	CCWGG	5 cut(s)	27, 413, 517, 622, 820
PspN4I	GGNNCC	5 cut(s)	33, 76, 412, 550, 811
PspPI	GGNCC	2 cut(s)	222, 524
PsuI	RGATCY	2 cut(s)	635, 724
PsyI	GACNNNGTC	1 cut(s)	56
PvuI	CGATCG	1 cut(s)	160
RsaI	GTAC	1 cut(s)	343
RsaNI	GTAC	1 cut(s)	342
SacI	GAGCTC	1 cut(s)	240
SaqAI	TTAA	3 cut(s)	392, 477, 501
SatI	GCNGC	5 cut(s)	127, 141, 144, 151, 209
Sau3AI	GATC	5 cut(s)	157, 467, 600, 635, 724
Sau96I	GGNCC	2 cut(s)	222, 524
SchI	GAGTC	1 cut(s)	758
ScrFI	CCNGG	5 cut(s)	29, 415, 519, 624, 822
SduI	GDGCHC	2 cut(s)	240, 595
SexAI	ACCWGGT	1 cut(s)	413
SfaNI	GCATC	3 cut(s)	283, 315, 359
SfoI	GGCGCC	1 cut(s)	76
SgrAI	CRCCGGYG	2 cut(s)	71, 329
SinI	GGWCC	1 cut(s)	524
SpeI	ACTAGT	1 cut(s)	742
SphI	GCATGC	1 cut(s)	448
Sse9I	AATT	3 cut(s)	362, 535, 642
SsiI	CCGC	6 cut(s)	141, 144, 151, 204, 209, 230
SspDI	GGCGCC	1 cut(s)	74
SspMI	CTAG	1 cut(s)	743
SstI	GAGCTC	1 cut(s)	240
StyD4I	CCNGG	5 cut(s)	27, 413, 517, 622, 820
StyI	CCWWGG	2 cut(s)	35, 567
TaaI	ACNGT	2 cut(s)	346, 697
TaiI	ACGT	2 cut(s)	246, 392
TaqI	TCGA	4 cut(s)	108, 156, 240, 309
TasI	AATT	3 cut(s)	362, 535, 642
TauI	GCSGC	4 cut(s)	143, 146, 153, 211
Tru1I	TTAA	3 cut(s)	392, 477, 501
Tru9I	TTAA	3 cut(s)	392, 477, 501
TseFI	GTSAC	2 cut(s)	67, 176
TseI	GCWGC	1 cut(s)	126
Tsp45I	GTSAC	2 cut(s)	67, 176
Tth111I	GACNNNGTC	1 cut(s)	56
VpaK11BI	GGWCC	1 cut(s)	524
VspI	ATTAAT	1 cut(s)	477
XceI	RCATGY	1 cut(s)	448
XmiI	GTMKAC	1 cut(s)	801
XspI	CTAG	1 cut(s)	743
ZraI	GACGTC	1 cut(s)	244
Zsp2I	ATGCAT	1 cut(s)	446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.