Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002630

Physical Location & Seq

Genomic Coordinates Forward (+)

773358 .. 777025

Gene Structure 3668 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1006600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGGAGAATTCTCATCAAGTGTCGGACCACCTGGAGCCGTGGCGCCACCTCCACGGTAAGGTTGTCATGGTCACCGGCGCTTCCTCTGGTCTCGGCCGCGACTTTTGCTTAGACTTGGCTAAAGCCGGATGCAGAATCGTAGCCGCCGCTCGCCGCATCGACCGCCTTCAGTCCGTTTGCGATCAAATCAACCAGCTCTCCATTACCGCTCCGTCTTTTTCTTCTTCTGCTGTTGCTGATAATATTCCTGGAGGTGGCCCGCGGGCCGTGGCTGTTGAGCTGGATGTCAGCGCCGATGGCCCAGCTATTGAGAAGTCTGTGAACAAGGCTTGGGACGCGTTTGGGCGCATCGATGCCCTTGTTAACAATGCCGGTATTAGAGGTAATGTGAATTCTGCATTGGAATTGTCTGAGGAGGAATGGAATCATGTTTTCAGGACAAATTTAACGGGTTCCTGGTTGGTTTCAAAGTTTGTTGCTATACGCATGCGAGATGCTGATCAGGGAGGATCAATCGTCAATATATCTTCCATTGCTGGTATTAATCGTGGACATTTGCCTGGAGGTACAGCATACATTTGTTCAAAGGCTGGCGTAAACACCTTAACAAAGACTATGGCTATGGAGTTGGGGGTGCACAAAATCAGAGTGAACGCAATATCACCTGGACTTTTCAGGTCGGAAATTACGGAGGGTCTTATGCAAAAAAAATGGATACCCAATGTGGCTATGAAAATTGTCCCTTTACGAACATATGGGACTTCGGATCCAGCATTGACATCGCTACTTAGATATTTGGTACATGACTCTTCTGAATATGTGTCTGGCAACATTTACATTGTTGATGCAGGAACCACCTTGCCAGGTGTTCCTATTTTCTCTTCCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004312 GO:0004316 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0030497 GO:0032787 GO:0036094 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0048037 GO:0050661 GO:0050662 GO:0055114 GO:0071704 GO:0072330 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

adh_short adh_short_C2

Protein Analysis

297

Amino Acids

31.69

Weight (kDa)

7.74

Isoelectric Point (pI)

35.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	20 - 67	3.9e-09	short chain dehydrogenase
KR	PF08659	22 - 201	2e-08	KR domain
adh_short	PF00106	77 - 236	1.5e-38	short chain dehydrogenase
adh_short_C2	PF13561	86 - 286	3.6e-40	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62610 AT1G62610 AT1G62610 AT1G62610 AT1G63380 AT1G63380 AT1G63380 AT1G63380 AT2G17845 AT3G46170 AT3G55290 AT3G55290 AT3G55310
fragaria_vesca	FvH4_5g32470
malus_domestica	MD12G1006600.v1.1 MD12G1094700.v1.1 MD15G1420800.v1.1 MD15G1420900.v1.1
prunus_persica	Prupe.7G031300_v2.0.a1 Prupe.7G031300_v2.0.a1
pyrus_communis	pycom13g09640 pycom14g08370
rosa_chinensis	RchiOBHm_Chr7g0229021 RchiOBHm_Chr7g0229061
rosa_laevigata	RLG00000001534
rosa_multiflora	Rmu_sc0000271.1_g000029 Rmu_sc0000271.1_g000037 Rmu_sc0009986.1_g000002
rosa_roxburghii	Rroxscaffold_3G00230780
rosa_rugosa	Rorug07G0254900 Rorug07G0255400
rosa_samantha	Rh7AG406300 Rh7AG406400 Rh7BG385600 Rh7BG385700 Rh7CG423900 Rh7CG424100 Rh7DG400700
rosa_wichuraiana	Rw7G033710 Rw7G033730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	42
AccBSI	CCGCTC	2 cut(s)	149, 209
AccII	CGCG	3 cut(s)	99, 262, 338
AciI	CCGC	8 cut(s)	97, 144, 147, 154, 163, 207, 260, 262
AclWI	GGATC	3 cut(s)	517, 761, 774
AcoI	YGGCCR	1 cut(s)	94
AcsI	RAATTY	3 cut(s)	7, 391, 442
AcuI	CTGAAG	1 cut(s)	152
AcyI	GRCGYC	1 cut(s)	43
AfaI	GTAC	2 cut(s)	568, 801
AfiI	CCNNNNNNNGG	2 cut(s)	58, 254
AflIII	ACRYGT	1 cut(s)	336
AgsI	TTSAA	2 cut(s)	468, 585
AjnI	CCWGG	6 cut(s)	30, 247, 455, 559, 664, 862
AjuI	GAANNNNNNNTTGG	2 cut(s)	383, 415
AluBI	AGCT	3 cut(s)	196, 280, 305
AluI	AGCT	3 cut(s)	196, 280, 305
Alw21I	GWGCWC	1 cut(s)	639
Alw26I	GTCTC	1 cut(s)	95
Alw44I	GTGCAC	1 cut(s)	635
AlwI	GGATC	3 cut(s)	517, 761, 774
AoxI	GGCC	4 cut(s)	94, 256, 264, 298
ApaLI	GTGCAC	1 cut(s)	635
ApoI	RAATTY	3 cut(s)	7, 391, 442
AseI	ATTAAT	1 cut(s)	543
AspLEI	GCGC	4 cut(s)	45, 80, 293, 348
AspS9I	GGNCC	4 cut(s)	25, 257, 264, 299
AsuHPI	GGTGA	2 cut(s)	64, 654
AvaII	GGWCC	1 cut(s)	25
BaeGI	GKGCMC	1 cut(s)	639
BamHI	GGATCC	1 cut(s)	766
BanI	GGYRCC	1 cut(s)	42
Bbv12I	GWGCWC	1 cut(s)	639
BccI	CCATC	1 cut(s)	290
BceAI	ACGGC	2 cut(s)	22, 251
BcgI	CGANNNNNNTGC	2 cut(s)	762, 796
BciT130I	CCWGG	6 cut(s)	32, 249, 457, 561, 666, 864
BciVI	GTATCC	1 cut(s)	708
BclI	TGATCA	1 cut(s)	499
BcoDI	GTCTC	1 cut(s)	95
BfoI	RGCGCY	3 cut(s)	46, 81, 294
BfuI	GTATCC	1 cut(s)	708
BisI	GCNGC	4 cut(s)	97, 144, 147, 154
BlsI	GCNGC	4 cut(s)	98, 145, 148, 155
Bme1390I	CCNGG	6 cut(s)	32, 249, 457, 561, 666, 864
Bme18I	GGWCC	1 cut(s)	25
BmgT120I	GGNCC	4 cut(s)	25, 257, 264, 299
BmiI	GGNNCC	5 cut(s)	36, 44, 454, 768, 853
BmrFI	CCNGG	6 cut(s)	32, 249, 457, 561, 666, 864
BmsI	GCATC	6 cut(s)	119, 165, 343, 357, 484, 835
BpmI	CTGGAG	3 cut(s)	53, 270, 582
Bsa29I	ATCGAT	1 cut(s)	351
BsaBI	GATNNNNATC	1 cut(s)	498
BsaHI	GRCGYC	1 cut(s)	43
BsaI	GGTCTC	1 cut(s)	95
BsaJI	CCNNGG	4 cut(s)	38, 52, 260, 267
BsaXI	ACNNNNNCTCC	2 cut(s)	182, 212
Bsc4I	CCNNNNNNNGG	2 cut(s)	58, 254
Bse118I	RCCGGY	2 cut(s)	74, 371
Bse3DI	GCAATG	1 cut(s)	531
Bse8I	GATNNNNATC	1 cut(s)	498
BseBI	CCWGG	6 cut(s)	32, 249, 457, 561, 666, 864
BseCI	ATCGAT	1 cut(s)	351
BseDI	CCNNGG	4 cut(s)	38, 52, 260, 267
BseGI	GGATG	2 cut(s)	134, 289
BseJI	GATNNNNATC	1 cut(s)	498
BseLI	CCNNNNNNNGG	2 cut(s)	58, 254
BseMI	GCAATG	1 cut(s)	531
BseMII	CTCAG	1 cut(s)	402
BseRI	GAGGAG	1 cut(s)	428
BseSI	GKGCMC	1 cut(s)	639
BseX3I	CGGCCG	1 cut(s)	94
BseYI	CCCAGC	1 cut(s)	301
Bsh1236I	CGCG	3 cut(s)	99, 262, 338
Bsh1285I	CGRYCG	2 cut(s)	97, 163
BshFI	GGCC	4 cut(s)	96, 258, 266, 300
BshNI	GGYRCC	1 cut(s)	42
BshVI	ATCGAT	1 cut(s)	351
BsiEI	CGRYCG	2 cut(s)	97, 163
BsiHKAI	GWGCWC	1 cut(s)	639
BsiSI	CCGG	3 cut(s)	75, 126, 372
BslFI	GGGAC	3 cut(s)	347, 725, 772
BslI	CCNNNNNNNGG	2 cut(s)	58, 254
BsmAI	GTCTC	1 cut(s)	95
BsmFI	GGGAC	3 cut(s)	347, 725, 772
BsnI	GGCC	4 cut(s)	96, 258, 266, 300
Bso31I	GGTCTC	1 cut(s)	95
Bsp1286I	GDGCHC	1 cut(s)	639
Bsp143I	GATC	4 cut(s)	181, 499, 509, 766
BspACI	CCGC	8 cut(s)	97, 144, 147, 154, 163, 207, 260, 262
BspANI	GGCC	4 cut(s)	96, 258, 266, 300
BspCNI	CTCAG	1 cut(s)	403
BspDI	ATCGAT	1 cut(s)	351
BspFNI	CGCG	3 cut(s)	99, 262, 338
BspLI	GGNNCC	5 cut(s)	36, 44, 454, 768, 853
BspPI	GGATC	3 cut(s)	517, 761, 774
BspT107I	GGYRCC	1 cut(s)	42
BspTNI	GGTCTC	1 cut(s)	95
BsrBI	CCGCTC	2 cut(s)	149, 209
BsrDI	GCAATG	1 cut(s)	531
BsrFI	RCCGGY	2 cut(s)	74, 371
BssAI	RCCGGY	2 cut(s)	74, 371
BssECI	CCNNGG	4 cut(s)	38, 52, 260, 267
BssMI	GATC	4 cut(s)	181, 499, 509, 766
BssNI	GRCGYC	1 cut(s)	43
Bst2UI	CCWGG	6 cut(s)	32, 249, 457, 561, 666, 864
Bst4CI	ACNGT	1 cut(s)	56
Bst6I	CTCTTC	2 cut(s)	814, 886
BstACI	GRCGYC	1 cut(s)	43
BstC8I	GCNNGC	5 cut(s)	151, 260, 264, 488, 592
BstDEI	CTNAG	3 cut(s)	109, 411, 788
BstDSI	CCRYGG	4 cut(s)	38, 52, 260, 267
BstEII	GGTNACC	1 cut(s)	70
BstF5I	GGATG	2 cut(s)	134, 289
BstFNI	CGCG	3 cut(s)	99, 262, 338
BstH2I	RGCGCY	3 cut(s)	46, 81, 294
BstHHI	GCGC	4 cut(s)	45, 80, 293, 348
BstKTI	GATC	4 cut(s)	184, 502, 512, 769
BstMAI	GTCTC	1 cut(s)	95
BstMBI	GATC	4 cut(s)	181, 499, 509, 766
BstMCI	CGRYCG	2 cut(s)	97, 163
BstMWI	GCNNNNNNNGC	4 cut(s)	105, 162, 297, 335
BstNI	CCWGG	6 cut(s)	32, 249, 457, 561, 666, 864
BstNSI	RCATGY	1 cut(s)	490
BstPI	GGTNACC	1 cut(s)	70
BstSCI	CCNGG	6 cut(s)	30, 247, 455, 559, 664, 862
BstSLI	GKGCMC	1 cut(s)	639
BstUI	CGCG	3 cut(s)	99, 262, 338
BstX2I	RGATCY	1 cut(s)	766
BstYI	RGATCY	1 cut(s)	766
BstZI	CGGCCG	1 cut(s)	94
Bsu15I	ATCGAT	1 cut(s)	351
BsuI	GTATCC	1 cut(s)	708
BsuRI	GGCC	4 cut(s)	96, 258, 266, 300
BsuTUI	ATCGAT	1 cut(s)	351
BtgI	CCRYGG	4 cut(s)	38, 52, 260, 267
BtgZI	GCGATG	1 cut(s)	765
BtsCI	GGATG	2 cut(s)	134, 289
Cac8I	GCNNGC	5 cut(s)	151, 260, 264, 488, 592
CfoI	GCGC	4 cut(s)	45, 80, 293, 348
Cfr10I	RCCGGY	2 cut(s)	74, 371
Cfr13I	GGNCC	4 cut(s)	25, 257, 264, 299
Cfr42I	CCGCGG	1 cut(s)	263
ClaI	ATCGAT	1 cut(s)	351
CseI	GACGC	1 cut(s)	344
Csp6I	GTAC	2 cut(s)	567, 800
CviAII	CATG	4 cut(s)	67, 428, 487, 803
CviQI	GTAC	2 cut(s)	567, 800
DdeI	CTNAG	3 cut(s)	109, 411, 788
DinI	GGCGCC	1 cut(s)	44
DpnI	GATC	4 cut(s)	183, 501, 511, 768
DpnII	GATC	4 cut(s)	181, 499, 509, 766
EaeI	YGGCCR	1 cut(s)	94
EagI	CGGCCG	1 cut(s)	94
Eam1104I	CTCTTC	2 cut(s)	814, 886
EarI	CTCTTC	2 cut(s)	814, 886
EclXI	CGGCCG	1 cut(s)	94
Eco31I	GGTCTC	1 cut(s)	95
Eco47I	GGWCC	1 cut(s)	25
Eco52I	CGGCCG	1 cut(s)	94
Eco57I	CTGAAG	1 cut(s)	152
Eco91I	GGTNACC	1 cut(s)	70
EcoO65I	GGTNACC	1 cut(s)	70
EcoRI	GAATTC	2 cut(s)	7, 391
EcoRII	CCWGG	6 cut(s)	30, 247, 455, 559, 664, 862
EgeI	GGCGCC	1 cut(s)	44
EheI	GGCGCC	1 cut(s)	44
FaeI	CATG	4 cut(s)	70, 431, 490, 806
FaqI	GGGAC	3 cut(s)	347, 725, 772
FatI	CATG	4 cut(s)	66, 427, 486, 802
FauI	CCCGC	2 cut(s)	255, 267
FauNDI	CATATG	1 cut(s)	754
FbaI	TGATCA	1 cut(s)	499
Fnu4HI	GCNGC	4 cut(s)	97, 144, 147, 154
FokI	GGATG	2 cut(s)	141, 296
Fsp4HI	GCNGC	4 cut(s)	97, 144, 147, 154
GlaI	GCGC	4 cut(s)	44, 79, 292, 347
GluI	GCNGC	4 cut(s)	97, 144, 147, 154
GsaI	CCCAGC	1 cut(s)	305
GsuI	CTGGAG	3 cut(s)	53, 270, 582
HaeII	RGCGCY	3 cut(s)	46, 81, 294
HaeIII	GGCC	4 cut(s)	96, 258, 266, 300
HapII	CCGG	3 cut(s)	75, 126, 372
HgaI	GACGC	1 cut(s)	344
HhaI	GCGC	4 cut(s)	45, 80, 293, 348
Hin1I	GRCGYC	1 cut(s)	43
Hin1II	CATG	4 cut(s)	70, 431, 490, 806
Hin6I	GCGC	4 cut(s)	43, 78, 291, 346
HinP1I	GCGC	4 cut(s)	43, 78, 291, 346
HincII	GTYRAC	1 cut(s)	364
HindII	GTYRAC	1 cut(s)	364
HinfI	GANTC	3 cut(s)	135, 424, 806
HpaI	GTTAAC	1 cut(s)	364
HpaII	CCGG	3 cut(s)	75, 126, 372
HphI	GGTGA	2 cut(s)	64, 654
Hpy166II	GTNNAC	6 cut(s)	322, 364, 551, 598, 637, 652
Hpy188I	TCNGA	7 cut(s)	25, 412, 647, 682, 766, 814, 890
Hpy188III	TCNNGA	1 cut(s)	436
Hpy8I	GTNNAC	6 cut(s)	322, 364, 551, 598, 637, 652
HpyAV	CCTTC	1 cut(s)	176
HpyCH4III	ACNGT	1 cut(s)	56
HpyCH4V	TGCA	5 cut(s)	132, 398, 637, 703, 848
HpyF10VI	GCNNNNNNNGC	4 cut(s)	105, 162, 297, 335
HpyF3I	CTNAG	3 cut(s)	109, 411, 788
Hsp92I	GRCGYC	1 cut(s)	43
Hsp92II	CATG	4 cut(s)	70, 431, 490, 806
HspAI	GCGC	4 cut(s)	43, 78, 291, 346
KasI	GGCGCC	1 cut(s)	42
Ksp22I	TGATCA	1 cut(s)	499
KspAI	GTTAAC	1 cut(s)	364
KspI	CCGCGG	1 cut(s)	263
Kzo9I	GATC	4 cut(s)	181, 499, 509, 766
LmnI	GCTCC	2 cut(s)	34, 214
LweI	GCATC	6 cut(s)	119, 165, 343, 357, 484, 835
MaeIII	GTNAC	1 cut(s)	70
MalI	GATC	4 cut(s)	183, 501, 511, 768
MbiI	CCGCTC	2 cut(s)	149, 209
MboI	GATC	4 cut(s)	181, 499, 509, 766
MboII	GAAGA	5 cut(s)	213, 216, 519, 801, 873
MflI	RGATCY	1 cut(s)	766
MhlI	GDGCHC	1 cut(s)	639
MluCI	AATT	6 cut(s)	7, 391, 404, 442, 684, 735
MluI	ACGCGT	1 cut(s)	336
Mly113I	GGCGCC	1 cut(s)	43
MlyI	GAGTC	1 cut(s)	800
MmeI	TCCRAC	1 cut(s)	660
MnlI	CCTC	9 cut(s)	59, 94, 245, 374, 406, 409, 500, 557, 685
MseI	TTAA	4 cut(s)	363, 446, 543, 605
MspA1I	CMGCKG	1 cut(s)	262
MspI	CCGG	3 cut(s)	75, 126, 372
MspR9I	CCNGG	6 cut(s)	32, 249, 457, 561, 666, 864
MvaI	CCWGG	6 cut(s)	32, 249, 457, 561, 666, 864
MvnI	CGCG	3 cut(s)	99, 262, 338
MwoI	GCNNNNNNNGC	4 cut(s)	105, 162, 297, 335
NarI	GGCGCC	1 cut(s)	43
NdeI	CATATG	1 cut(s)	754
NdeII	GATC	4 cut(s)	181, 499, 509, 766
NlaIII	CATG	4 cut(s)	70, 431, 490, 806
NlaIV	GGNNCC	5 cut(s)	36, 44, 454, 768, 853
NmeAIII	GCCGAG	1 cut(s)	72
NmuCI	GTSAC	1 cut(s)	70
NspI	RCATGY	1 cut(s)	490
PaeI	GCATGC	1 cut(s)	490
PfeI	GAWTC	2 cut(s)	135, 424
PfoI	TCCNGGA	1 cut(s)	247
PkrI	GCNGC	4 cut(s)	98, 145, 148, 155
PleI	GAGTC	1 cut(s)	800
PluTI	GGCGCC	1 cut(s)	46
PpsI	GAGTC	1 cut(s)	800
PshBI	ATTAAT	1 cut(s)	543
Psp6I	CCWGG	6 cut(s)	30, 247, 455, 559, 664, 862
PspEI	GGTNACC	1 cut(s)	70
PspFI	CCCAGC	1 cut(s)	301
PspGI	CCWGG	6 cut(s)	30, 247, 455, 559, 664, 862
PspN4I	GGNNCC	5 cut(s)	36, 44, 454, 768, 853
PspPI	GGNCC	4 cut(s)	25, 257, 264, 299
PsuI	RGATCY	1 cut(s)	766
RsaI	GTAC	2 cut(s)	568, 801
RsaNI	GTAC	2 cut(s)	567, 800
SacII	CCGCGG	1 cut(s)	263
SaqAI	TTAA	4 cut(s)	363, 446, 543, 605
SatI	GCNGC	4 cut(s)	97, 144, 147, 154
Sau3AI	GATC	4 cut(s)	181, 499, 509, 766
Sau96I	GGNCC	4 cut(s)	25, 257, 264, 299
SchI	GAGTC	1 cut(s)	800
ScrFI	CCNGG	6 cut(s)	32, 249, 457, 561, 666, 864
SduI	GDGCHC	1 cut(s)	639
SfaNI	GCATC	6 cut(s)	119, 165, 343, 357, 484, 835
SfoI	GGCGCC	1 cut(s)	44
Sfr303I	CCGCGG	1 cut(s)	263
SgrAI	CRCCGGYG	1 cut(s)	74
SgrBI	CCGCGG	1 cut(s)	263
SinI	GGWCC	1 cut(s)	25
SphI	GCATGC	1 cut(s)	490
Sse9I	AATT	6 cut(s)	7, 391, 404, 442, 684, 735
SsiI	CCGC	8 cut(s)	97, 144, 147, 154, 163, 207, 260, 262
SspDI	GGCGCC	1 cut(s)	42
SspI	AATATT	1 cut(s)	244
StyD4I	CCNGG	6 cut(s)	30, 247, 455, 559, 664, 862
TaaI	ACNGT	1 cut(s)	56
TaqI	TCGA	2 cut(s)	159, 351
TasI	AATT	6 cut(s)	7, 391, 404, 442, 684, 735
TauI	GCSGC	4 cut(s)	99, 146, 149, 156
TfiI	GAWTC	2 cut(s)	135, 424
Tru1I	TTAA	4 cut(s)	363, 446, 543, 605
Tru9I	TTAA	4 cut(s)	363, 446, 543, 605
TseFI	GTSAC	1 cut(s)	70
Tsp45I	GTSAC	1 cut(s)	70
TspDTI	ATGAA	1 cut(s)	746
TspGWI	ACGGA	3 cut(s)	163, 201, 704
VneI	GTGCAC	1 cut(s)	635
VpaK11BI	GGWCC	1 cut(s)	25
VspI	ATTAAT	1 cut(s)	543
XapI	RAATTY	3 cut(s)	7, 391, 442
XceI	RCATGY	1 cut(s)	490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.