FvH4_5g32910

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006935
Physical Location & Seq
Reverse (-)
23755551 .. 23761214
5664 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32910.t2

Sequence Viewer

Length: 1206 bp
ATGAAATTCGCAGTGAAGGCATGGAGCAGTGGAAGAGCACGGGCAGGAGTGCTCCATCTGGGCAGCTGCCCCAGCCCAATAGAGACCCCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTCCCCCTTTTCATCCCCCCTGACCTTCTCCCGTCTCTCCCTTCCCCTGATTCCCATCTCCTCCATGTTAGCTCCCTCCACTTCTTGGAAGGCATCTCGCCCAATACAATATCGAAAATAGGAGGATTGCATAAACTGGTGGGTTTGCCGGACCACGGATTCGTAGCCATTCCGAGAGACTCTATTCAATCCCTCCCGGAATGTAATAGCACTAATAAATTGGGAGCATCTTTCGACACTCCCGTTGGTCGCAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTCTTCATTGAAGCCTAATGTATGGACCGCTTTGGCGGACGAAGTCCCTGCATGGGTAAATGATAAGAGGAACAAGACCTCGGTTGATCGAACTGTTAGATGGCTTGACGAATGCATTCAGCTAAATCAGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCGCAAGAGGTGGCCAGCCGAAGTGTATCAGGTTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATCTTAGAAGAAGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAGGAGGTCTTGCAGGGTGTTGCTGCAGGAGTTGATATTTTTGACTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATGTCTCTGCTCTTCACTTAAGTAATATAGGAAGTGATCAGAGCAAGATTAATTTAAGAGCAACAATTTACAGGAAAGACACTACACCAATTGTTGAAAATTGTAACTGTTACACATGCCAGAATCATACAAGAGCATACATTAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATACATAATACTCACCACTATCTAGGTTTCTTCCGCTCAGTAAGGGAGGCAATTAAGTCCGGTAATTTTGAGCAATTCAGACAGAAGTTTTCTGAGATTAAGCGTTATCATATGCATCTTGCTTCTGAAGCTGCTGTTGGCTGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

402

Amino Acids

43.94

Weight (kDa)

6.35

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 359 7e-75 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1023
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 2 cut(s) 527, 1023
AccB7I CCANNNNNTGG 2 cut(s) 202, 851
AccBSI CCGCTC 1 cut(s) 1092
AciI CCGC 3 cut(s) 432, 440, 1090
AclWI GGATC 4 cut(s) 549, 550, 562, 577
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 1 cut(s) 1203
AfiI CCNNNNNNNGG 4 cut(s) 202, 272, 457, 851
AflII CTTAAG 1 cut(s) 883
AgsI TTSAA 6 cut(s) 305, 398, 415, 581, 962, 1055
AjuI GAANNNNNNNTTGG 3 cut(s) 710, 742, 1176
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 5 cut(s) 66, 189, 526, 1040, 1187
AluI AGCT 5 cut(s) 66, 189, 526, 1040, 1187
Alw21I GWGCWC 2 cut(s) 40, 54
Alw26I GTCTC 5 cut(s) 77, 156, 288, 854, 874
AlwI GGATC 4 cut(s) 549, 550, 562, 577
AoxI GGCC 2 cut(s) 115, 609
ApeKI GCWGC 4 cut(s) 63, 66, 773, 1187
ApoI RAATTY 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 2 cut(s) 915, 1008
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 601, 675
AspS9I GGNCC 3 cut(s) 115, 268, 429
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 2 cut(s) 1004, 1061
AsuNHI GCTAGC 1 cut(s) 1036
AvaII GGWCC 2 cut(s) 268, 429
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 554
Bbv12I GWGCWC 2 cut(s) 40, 54
BbvI GCAGC 4 cut(s) 53, 75, 760, 1174
BccI CCATC 5 cut(s) 63, 180, 498, 819, 845
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 5 cut(s) 77, 156, 288, 854, 874
BfaI CTAG 3 cut(s) 848, 1037, 1079
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfrI CTTAAG 1 cut(s) 883
BfuAI ACCTGC 2 cut(s) 527, 1023
BisI GCNGC 4 cut(s) 64, 67, 774, 1188
BlsI GCNGC 4 cut(s) 65, 68, 775, 1189
Bme1390I CCNGG 1 cut(s) 314
Bme18I GGWCC 2 cut(s) 268, 429
BmgT120I GGNCC 3 cut(s) 115, 268, 429
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 3 cut(s) 219, 353, 1180
BmtI GCTAGC 1 cut(s) 1040
BoxI GACNNNNGTC 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 314
BsaAI YACGTR 1 cut(s) 801
BsaBI GATNNNNATC 1 cut(s) 171
BsaI GGTCTC 2 cut(s) 77, 854
BsaJI CCNNGG 3 cut(s) 271, 483, 820
BsaWI WCCGGW 1 cut(s) 1115
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 272, 457, 851
Bse1I ACTGG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 171
BseDI CCNNGG 3 cut(s) 271, 483, 820
BseGI GGATG 2 cut(s) 129, 673
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 4 cut(s) 202, 272, 457, 851
BseMII CTCAG 3 cut(s) 1055, 1107, 1140
BseNI ACTGG 1 cut(s) 258
BseRI GAGGAG 3 cut(s) 89, 167, 767
BseXI GCAGC 4 cut(s) 53, 75, 760, 1174
BseYI CCCAGC 1 cut(s) 71
BshFI GGCC 2 cut(s) 117, 611
BsiHKAI GWGCWC 2 cut(s) 40, 54
BsiSI CCGG 3 cut(s) 266, 314, 1116
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 4 cut(s) 202, 272, 457, 851
BsmAI GTCTC 5 cut(s) 77, 156, 288, 854, 874
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 2 cut(s) 519, 521
BsnI GGCC 2 cut(s) 117, 611
Bso31I GGTCTC 2 cut(s) 77, 854
Bsp1286I GDGCHC 2 cut(s) 40, 54
Bsp143I GATC 5 cut(s) 490, 542, 554, 569, 901
BspACI CCGC 3 cut(s) 432, 440, 1090
BspANI GGCC 2 cut(s) 117, 611
BspCNI CTCAG 3 cut(s) 1054, 1106, 1141
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 527, 1023
BspOI GCTAGC 1 cut(s) 1040
BspPI GGATC 4 cut(s) 549, 550, 562, 577
BspQI GCTCTTC 3 cut(s) 28, 99, 882
BspTI CTTAAG 1 cut(s) 883
BspTNI GGTCTC 2 cut(s) 77, 854
BsrBI CCGCTC 1 cut(s) 1092
BsrI ACTGG 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 271, 483, 820
BssMI GATC 5 cut(s) 490, 542, 554, 569, 901
BssT1I CCWWGG 1 cut(s) 820
Bst4CI ACNGT 2 cut(s) 499, 974
Bst6I CTCTTC 4 cut(s) 28, 99, 708, 882
BstAFI CTTAAG 1 cut(s) 883
BstBAI YACGTR 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 613, 1038
BstDEI CTNAG 4 cut(s) 706, 1041, 1093, 1149
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 129, 673
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 601, 675
BstKTI GATC 5 cut(s) 493, 545, 557, 572, 904
BstMAI GTCTC 5 cut(s) 77, 156, 288, 854, 874
BstMBI GATC 5 cut(s) 490, 542, 554, 569, 901
BstMWI GCNNNNNNNGC 4 cut(s) 17, 72, 598, 1184
BstNSI RCATGY 1 cut(s) 984
BstPAI GACNNNNGTC 1 cut(s) 866
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 2 cut(s) 384, 774
BstSNI TACGTA 1 cut(s) 801
BstV1I GCAGC 4 cut(s) 53, 75, 760, 1174
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstYI RGATCY 3 cut(s) 542, 554, 569
BsuRI GGCC 2 cut(s) 117, 611
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 129, 673
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 3 cut(s) 18, 34, 739
BveI ACCTGC 2 cut(s) 527, 1023
Cac8I GCNNGC 2 cut(s) 613, 1038
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 2 cut(s) 601, 675
Cfr13I GGNCC 3 cut(s) 115, 268, 429
CviAII CATG 6 cut(s) 21, 182, 456, 664, 981, 1028
DdeI CTNAG 4 cut(s) 706, 1041, 1093, 1149
DpnI GATC 5 cut(s) 492, 544, 556, 571, 903
DpnII GATC 5 cut(s) 490, 542, 554, 569, 901
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 4 cut(s) 28, 99, 708, 882
EarI CTCTTC 4 cut(s) 28, 99, 708, 882
EciI GGCGGA 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 801
Eco130I CCWWGG 1 cut(s) 820
Eco31I GGTCTC 2 cut(s) 77, 854
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 268, 429
Eco57I CTGAAG 1 cut(s) 1203
EcoO109I RGGNCCY 1 cut(s) 115
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 820
EcoT22I ATGCAT 2 cut(s) 521, 1173
ErhI CCWWGG 1 cut(s) 820
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 6 cut(s) 24, 185, 459, 667, 984, 1031
FaqI GGGAC 1 cut(s) 434
FatI CATG 6 cut(s) 20, 181, 455, 663, 980, 1027
FauNDI CATATG 1 cut(s) 1167
FbaI TGATCA 1 cut(s) 901
Fnu4HI GCNGC 4 cut(s) 64, 67, 774, 1188
FokI GGATG 2 cut(s) 116, 680
Fsp4HI GCNGC 4 cut(s) 64, 67, 774, 1188
FspBI CTAG 3 cut(s) 848, 1037, 1079
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 600, 674
GluI GCNGC 4 cut(s) 64, 67, 774, 1188
GsaI CCCAGC 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 2 cut(s) 117, 611
HapII CCGG 3 cut(s) 266, 314, 1116
HhaI GCGC 2 cut(s) 601, 675
Hin1II CATG 6 cut(s) 24, 185, 459, 667, 984, 1031
Hin6I GCGC 2 cut(s) 599, 673
HinP1I GCGC 2 cut(s) 599, 673
HinfI GANTC 6 cut(s) 167, 276, 296, 794, 988, 1045
HpaII CCGG 3 cut(s) 266, 314, 1116
HphI GGTGA 2 cut(s) 1004, 1061
Hpy188I TCNGA 6 cut(s) 291, 906, 1044, 1136, 1150, 1183
Hpy188III TCNNGA 3 cut(s) 581, 749, 1028
HpyAV CCTTC 5 cut(s) 10, 152, 168, 200, 850
HpyCH4III ACNGT 2 cut(s) 499, 974
HpyCH4IV ACGT 2 cut(s) 586, 800
HpyCH4V TGCA 7 cut(s) 247, 455, 519, 763, 776, 833, 1171
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 72, 598, 1184
HpyF3I CTNAG 4 cut(s) 706, 1041, 1093, 1149
HpySE526I ACGT 2 cut(s) 586, 800
Hsp92II CATG 6 cut(s) 24, 185, 459, 667, 984, 1031
HspAI GCGC 2 cut(s) 599, 673
Ksp22I TGATCA 1 cut(s) 901
Kzo9I GATC 5 cut(s) 490, 542, 554, 569, 901
LguI GCTCTTC 3 cut(s) 28, 99, 882
LmnI GCTCC 4 cut(s) 24, 57, 194, 341
Lsp1109I GCAGC 4 cut(s) 53, 75, 760, 1174
LweI GCATC 3 cut(s) 219, 353, 1180
MaeI CTAG 3 cut(s) 848, 1037, 1079
MaeII ACGT 2 cut(s) 586, 800
MaeIII GTNAC 3 cut(s) 694, 968, 974
MalI GATC 5 cut(s) 492, 544, 556, 571, 903
MbiI CCGCTC 1 cut(s) 1092
MboI GATC 5 cut(s) 490, 542, 554, 569, 901
MboII GAAGA 8 cut(s) 45, 86, 399, 564, 722, 725, 869, 1078
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 2 cut(s) 40, 54
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 2 cut(s) 290, 788
MmeI TCCRAC 1 cut(s) 544
Mox20I TGGCCA 1 cut(s) 611
Mph1103I ATGCAT 2 cut(s) 521, 1173
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 611
MseI TTAA 8 cut(s) 378, 687, 884, 915, 920, 1008, 1110, 1155
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 66
MspCI CTTAAG 1 cut(s) 883
MspI CCGG 3 cut(s) 266, 314, 1116
MspR9I CCNGG 1 cut(s) 314
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 2 cut(s) 519, 521
MwoI GCNNNNNNNGC 4 cut(s) 17, 72, 598, 1184
NciI CCSGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 1167
NdeII GATC 5 cut(s) 490, 542, 554, 569, 901
NheI GCTAGC 1 cut(s) 1036
NlaIII CATG 6 cut(s) 24, 185, 459, 667, 984, 1031
NlaIV GGNNCC 1 cut(s) 556
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 2 cut(s) 521, 1173
NspI RCATGY 1 cut(s) 984
PagI TCATGA 1 cut(s) 1027
PaqCI CACCTGC 2 cut(s) 527, 1023
PciSI GCTCTTC 3 cut(s) 28, 99, 882
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 2 cut(s) 519, 521
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 4 cut(s) 167, 276, 988, 1045
PflFI GACNNNGTC 1 cut(s) 446
PflMI CCANNNNNTGG 2 cut(s) 202, 851
PfoI TCCNGGA 1 cut(s) 312
PkrI GCNGC 4 cut(s) 65, 68, 775, 1189
PleI GAGTC 2 cut(s) 290, 788
PpsI GAGTC 2 cut(s) 290, 788
Ppu21I YACGTR 1 cut(s) 801
PshAI GACNNNNGTC 1 cut(s) 866
PshBI ATTAAT 2 cut(s) 915, 1008
PspFI CCCAGC 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 3 cut(s) 115, 268, 429
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 3 cut(s) 542, 554, 569
PsyI GACNNNGTC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 66
SapI GCTCTTC 3 cut(s) 28, 99, 882
SaqAI TTAA 8 cut(s) 378, 687, 884, 915, 920, 1008, 1110, 1155
SatI GCNGC 4 cut(s) 64, 67, 774, 1188
Sau3AI GATC 5 cut(s) 490, 542, 554, 569, 901
Sau96I GGNCC 3 cut(s) 115, 268, 429
SchI GAGTC 2 cut(s) 290, 788
ScrFI CCNGG 1 cut(s) 314
SduI GDGCHC 2 cut(s) 40, 54
SfaNI GCATC 3 cut(s) 219, 353, 1180
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 2 cut(s) 268, 429
SmlI CTYRAG 1 cut(s) 883
SmoI CTYRAG 1 cut(s) 883
SnaBI TACGTA 1 cut(s) 801
SsiI CCGC 3 cut(s) 432, 440, 1090
SspMI CTAG 3 cut(s) 848, 1037, 1079
StyD4I CCNGG 1 cut(s) 312
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 2 cut(s) 499, 974
TaiI ACGT 2 cut(s) 589, 803
TaqI TCGA 3 cut(s) 230, 351, 493
TfiI GAWTC 4 cut(s) 167, 276, 988, 1045
Tru1I TTAA 8 cut(s) 378, 687, 884, 915, 920, 1008, 1110, 1155
Tru9I TTAA 8 cut(s) 378, 687, 884, 915, 920, 1008, 1110, 1155
TscAI CASTG 3 cut(s) 18, 34, 739
TseI GCWGC 4 cut(s) 63, 66, 773, 1187
TspDTI ATGAA 5 cut(s) 17, 118, 399, 1016, 1044
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 3 cut(s) 18, 34, 739
Tth111I GACNNNGTC 1 cut(s) 446
Van91I CCANNNNNTGG 2 cut(s) 202, 851
Vha464I CTTAAG 1 cut(s) 883
VpaK11BI GGWCC 2 cut(s) 268, 429
VspI ATTAAT 2 cut(s) 915, 1008
XapI RAATTY 1 cut(s) 5
XceI RCATGY 1 cut(s) 984
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 3 cut(s) 848, 1037, 1079
Zsp2I ATGCAT 2 cut(s) 521, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.