Rh7DG410600

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006935
Physical Location & Seq
Reverse (-)
57705166 .. 57711676
6511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG410600.1

Sequence Viewer

Length: 1140 bp
ATGGAGACGAGTTGCAGAGTGAGGAAGAAGGAAACAAGCAGCAGTGGAAGAGCACGAGCAGGGGTACTCCAGCTGGGCAGCTGCCCGAACCCAATAGAGACCCCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTGCCCCTTTTCATACCCCGTGACCTTCTCCCTTCTCTTCCTTCCCCTGACTCCCATCTCCTCCATGTTAGCTCCCTTCACTTCTTAGAAGGCATCTCGACGCAGACAATATCGAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCGGGACCACGGATTCACTCCCTGTGGTCGCAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTCTTCGTTAAGGCCTAATATATGGAGTGCTTTGGCTGATGAAGTTCCTGCATGGGTGTCTGATAAGAGGAACATGACCTCGGTTGATCGAACAGTAAGATGGCTTGACGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCACAAGAGGTGGCCAGCCGAAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATCTTAGAAGAGGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAAGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATTTTTGATTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATCTCTCTGCTTTTCAGTTGACTAATATAGGAAGTGATCAGACAAAGATTAATTTGAGAGCAACAATGCACAGGAAAGACACTTCACCAATTGTTGAAAATTGTAAATGTTACACGTGCCAGAATCATACAAAAGCGTACATCAATCACCTGTTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATGCATAATACTCACCACTATCTAAGTTTCTTCCGCTTAGTATTGGAGGCAATTAAGTCTGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAAGCGATATCATCTTGCTTCTGAAGCTGCTGTTGCCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

379

Amino Acids

41.94

Weight (kDa)

6.71

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 17 - 365 9.5e-78 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 467
AccB7I CCANNNNNTGG 1 cut(s) 791
AciI CCGC 2 cut(s) 280, 1030
AclWI GGATC 4 cut(s) 489, 490, 502, 517
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 1 cut(s) 1082
AcuI CTGAAG 1 cut(s) 1137
AcvI CACGTG 1 cut(s) 921
AfaI GTAC 2 cut(s) 66, 944
AfiI CCNNNNNNNGG 1 cut(s) 791
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 5 cut(s) 338, 521, 902, 961, 995
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
AloI GAACNNNNNNTCC 2 cut(s) 501, 533
AluBI AGCT 6 cut(s) 73, 81, 204, 466, 980, 1121
AluI AGCT 6 cut(s) 73, 81, 204, 466, 980, 1121
Alw21I GWGCWC 2 cut(s) 55, 541
Alw26I GTCTC 2 cut(s) 92, 794
Alw44I GTGCAC 1 cut(s) 537
AlwI GGATC 4 cut(s) 489, 490, 502, 517
AoxI GGCC 3 cut(s) 130, 356, 549
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 5 cut(s) 39, 78, 81, 713, 1121
ApoI RAATTY 1 cut(s) 1082
AseI ATTAAT 1 cut(s) 855
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 2 cut(s) 130, 283
AsuHPI GGTGA 3 cut(s) 882, 944, 1001
AsuNHI GCTAGC 1 cut(s) 976
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 541
BalI TGGCCA 1 cut(s) 551
BamHI GGATCC 1 cut(s) 494
BauI CACGAG 1 cut(s) 54
BbrPI CACGTG 1 cut(s) 921
Bbv12I GWGCWC 2 cut(s) 55, 541
BbvI GCAGC 5 cut(s) 51, 68, 90, 700, 1108
BccI CCATC 4 cut(s) 195, 438, 759, 785
BclI TGATCA 1 cut(s) 841
BcoDI GTCTC 2 cut(s) 92, 794
BfaI CTAG 2 cut(s) 788, 977
BfmI CTRYAG 2 cut(s) 324, 714
BfoI RGCGCY 1 cut(s) 616
BfuAI ACCTGC 1 cut(s) 467
BisI GCNGC 5 cut(s) 40, 79, 82, 714, 1122
BlsI GCNGC 5 cut(s) 41, 80, 83, 715, 1123
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 130, 283
BmiI GGNNCC 2 cut(s) 284, 496
BmsI GCATC 2 cut(s) 234, 258
BmtI GCTAGC 1 cut(s) 980
BpmI CTGGAG 1 cut(s) 53
BsaAI YACGTR 2 cut(s) 741, 921
BsaI GGTCTC 2 cut(s) 92, 794
BsaJI CCNNGG 3 cut(s) 286, 423, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 791
BseDI CCNNGG 3 cut(s) 286, 423, 760
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 1 cut(s) 791
BseMII CTCAG 2 cut(s) 995, 1080
BseRI GAGGAG 3 cut(s) 104, 182, 668
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 5 cut(s) 51, 68, 90, 700, 1108
BseYI CCCAGC 2 cut(s) 73, 271
BshFI GGCC 3 cut(s) 132, 358, 551
BsiHKAI GWGCWC 2 cut(s) 55, 541
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 791
BsmAI GTCTC 2 cut(s) 92, 794
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 2 cut(s) 459, 461
BsnI GGCC 3 cut(s) 132, 358, 551
Bso31I GGTCTC 2 cut(s) 92, 794
Bsp1286I GDGCHC 2 cut(s) 55, 541
Bsp143I GATC 5 cut(s) 430, 482, 494, 509, 841
BspACI CCGC 2 cut(s) 280, 1030
BspANI GGCC 3 cut(s) 132, 358, 551
BspCNI CTCAG 2 cut(s) 994, 1081
BspHI TCATGA 1 cut(s) 967
BspLI GGNNCC 2 cut(s) 284, 496
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 1 cut(s) 467
BspOI GCTAGC 1 cut(s) 980
BspPI GGATC 4 cut(s) 489, 490, 502, 517
BspQI GCTCTTC 2 cut(s) 43, 114
BspTNI GGTCTC 2 cut(s) 92, 794
BssECI CCNNGG 3 cut(s) 286, 423, 760
BssMI GATC 5 cut(s) 430, 482, 494, 509, 841
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 760
Bst2BI CACGAG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 5 cut(s) 43, 114, 174, 645, 687
BstAPI GCANNNNNTGC 1 cut(s) 268
BstBAI YACGTR 2 cut(s) 741, 921
BstC8I GCNNGC 4 cut(s) 134, 553, 978, 1132
BstDEI CTNAG 6 cut(s) 217, 646, 981, 1019, 1033, 1089
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 613
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 5 cut(s) 433, 485, 497, 512, 844
BstMAI GTCTC 2 cut(s) 92, 794
BstMBI GATC 5 cut(s) 430, 482, 494, 509, 841
BstMWI GCNNNNNNNGC 4 cut(s) 268, 277, 1118, 1127
BstNSI RCATGY 1 cut(s) 1138
BstSFI CTRYAG 2 cut(s) 324, 714
BstSLI GKGCMC 1 cut(s) 541
BstSNI TACGTA 1 cut(s) 741
BstV1I GCAGC 5 cut(s) 51, 68, 90, 700, 1108
BstX2I RGATCY 3 cut(s) 482, 494, 509
BstYI RGATCY 3 cut(s) 482, 494, 509
BsuRI GGCC 3 cut(s) 132, 358, 551
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 547
BtsCI GGATG 1 cut(s) 613
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 679
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 4 cut(s) 134, 553, 978, 1132
CciI TCATGA 1 cut(s) 967
CfoI GCGC 1 cut(s) 615
Cfr13I GGNCC 2 cut(s) 130, 283
CseI GACGC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 65, 943
CviAII CATG 6 cut(s) 197, 396, 418, 604, 968, 1135
CviQI GTAC 2 cut(s) 65, 943
DdeI CTNAG 6 cut(s) 217, 646, 981, 1019, 1033, 1089
DpnI GATC 5 cut(s) 432, 484, 496, 511, 843
DpnII GATC 5 cut(s) 430, 482, 494, 509, 841
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 5 cut(s) 43, 114, 174, 645, 687
EarI CTCTTC 5 cut(s) 43, 114, 174, 645, 687
Eco105I TACGTA 1 cut(s) 741
Eco130I CCWWGG 1 cut(s) 760
Eco147I AGGCCT 1 cut(s) 358
Eco31I GGTCTC 2 cut(s) 92, 794
Eco32I GATATC 2 cut(s) 643, 1103
Eco47I GGWCC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 1137
Eco72I CACGTG 1 cut(s) 921
EcoO109I RGGNCCY 1 cut(s) 130
EcoRV GATATC 2 cut(s) 643, 1103
EcoT14I CCWWGG 1 cut(s) 760
EcoT22I ATGCAT 2 cut(s) 461, 1002
ErhI CCWWGG 1 cut(s) 760
FaeI CATG 6 cut(s) 200, 399, 421, 607, 971, 1138
FaqI GGGAC 1 cut(s) 296
FatI CATG 6 cut(s) 196, 395, 417, 603, 967, 1134
FauI CCCGC 1 cut(s) 273
FbaI TGATCA 1 cut(s) 841
Fnu4HI GCNGC 5 cut(s) 40, 79, 82, 714, 1122
FokI GGATG 1 cut(s) 620
Fsp4HI GCNGC 5 cut(s) 40, 79, 82, 714, 1122
FspBI CTAG 2 cut(s) 788, 977
GlaI GCGC 1 cut(s) 614
GluI GCNGC 5 cut(s) 40, 79, 82, 714, 1122
GsaI CCCAGC 2 cut(s) 77, 275
GsuI CTGGAG 1 cut(s) 53
HaeII RGCGCY 1 cut(s) 616
HaeIII GGCC 3 cut(s) 132, 358, 551
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 615
Hin1II CATG 6 cut(s) 200, 399, 421, 607, 971, 1138
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HincII GTYRAC 1 cut(s) 825
HindII GTYRAC 1 cut(s) 825
HinfI GANTC 5 cut(s) 182, 291, 734, 928, 985
HphI GGTGA 3 cut(s) 882, 944, 1001
Hpy166II GTNNAC 2 cut(s) 539, 825
Hpy188I TCNGA 6 cut(s) 406, 846, 984, 1076, 1090, 1117
Hpy188III TCNNGA 4 cut(s) 229, 521, 689, 968
Hpy8I GTNNAC 2 cut(s) 539, 825
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 7 cut(s) 22, 167, 174, 183, 215, 218, 790
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4IV ACGT 3 cut(s) 526, 740, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 277, 1118, 1127
HpyF3I CTNAG 6 cut(s) 217, 646, 981, 1019, 1033, 1089
HpySE526I ACGT 3 cut(s) 526, 740, 920
Hsp92II CATG 6 cut(s) 200, 399, 421, 607, 971, 1138
HspAI GCGC 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 5 cut(s) 430, 482, 494, 509, 841
LguI GCTCTTC 2 cut(s) 43, 114
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 5 cut(s) 51, 68, 90, 700, 1108
LweI GCATC 2 cut(s) 234, 258
MaeI CTAG 2 cut(s) 788, 977
MaeII ACGT 3 cut(s) 526, 740, 920
MaeIII GTNAC 3 cut(s) 152, 634, 914
MalI GATC 5 cut(s) 432, 484, 496, 511, 843
MboI GATC 5 cut(s) 430, 482, 494, 509, 841
MboII GAAGA 9 cut(s) 37, 60, 101, 161, 339, 504, 662, 704, 1018
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 3 cut(s) 482, 494, 509
MhlI GDGCHC 2 cut(s) 55, 541
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 9 cut(s) 338, 720, 856, 894, 904, 1047, 1060, 1070, 1082
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 484
Mox20I TGGCCA 1 cut(s) 551
Mph1103I ATGCAT 2 cut(s) 461, 1002
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 551
MseI TTAA 5 cut(s) 318, 353, 627, 855, 1050
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 2 cut(s) 73, 81
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 2 cut(s) 459, 461
MwoI GCNNNNNNNGC 4 cut(s) 268, 277, 1118, 1127
NdeII GATC 5 cut(s) 430, 482, 494, 509, 841
NheI GCTAGC 1 cut(s) 976
NlaIII CATG 6 cut(s) 200, 399, 421, 607, 971, 1138
NlaIV GGNNCC 2 cut(s) 284, 496
NmuCI GTSAC 1 cut(s) 152
NsiI ATGCAT 2 cut(s) 461, 1002
NspI RCATGY 1 cut(s) 1138
PagI TCATGA 1 cut(s) 967
PaqCI CACCTGC 1 cut(s) 467
PceI AGGCCT 1 cut(s) 358
PciSI GCTCTTC 2 cut(s) 43, 114
PctI GAATGC 2 cut(s) 459, 461
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 4 cut(s) 291, 734, 928, 985
PflMI CCANNNNNTGG 1 cut(s) 791
PkrI GCNGC 5 cut(s) 41, 80, 83, 715, 1123
PleI GAGTC 1 cut(s) 176
PmaCI CACGTG 1 cut(s) 921
PmlI CACGTG 1 cut(s) 921
PpsI GAGTC 1 cut(s) 176
Ppu21I YACGTR 2 cut(s) 741, 921
PshBI ATTAAT 1 cut(s) 855
PspCI CACGTG 1 cut(s) 921
PspFI CCCAGC 2 cut(s) 73, 271
PspN4I GGNNCC 2 cut(s) 284, 496
PspPI GGNCC 2 cut(s) 130, 283
PstI CTGCAG 1 cut(s) 718
PsuI RGATCY 3 cut(s) 482, 494, 509
PvuII CAGCTG 2 cut(s) 73, 81
RsaI GTAC 2 cut(s) 66, 944
RsaNI GTAC 2 cut(s) 65, 943
SapI GCTCTTC 2 cut(s) 43, 114
SaqAI TTAA 5 cut(s) 318, 353, 627, 855, 1050
SatI GCNGC 5 cut(s) 40, 79, 82, 714, 1122
Sau3AI GATC 5 cut(s) 430, 482, 494, 509, 841
Sau96I GGNCC 2 cut(s) 130, 283
SchI GAGTC 1 cut(s) 176
SduI GDGCHC 2 cut(s) 55, 541
SfaNI GCATC 2 cut(s) 234, 258
SfcI CTRYAG 2 cut(s) 324, 714
SinI GGWCC 1 cut(s) 283
SnaBI TACGTA 1 cut(s) 741
Sse9I AATT 9 cut(s) 338, 720, 856, 894, 904, 1047, 1060, 1070, 1082
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 2 cut(s) 280, 1030
SspMI CTAG 2 cut(s) 788, 977
StuI AGGCCT 1 cut(s) 358
StyI CCWWGG 1 cut(s) 760
TaaI ACNGT 1 cut(s) 439
TaiI ACGT 3 cut(s) 529, 743, 923
TaqI TCGA 3 cut(s) 230, 245, 433
TasI AATT 9 cut(s) 338, 720, 856, 894, 904, 1047, 1060, 1070, 1082
TfiI GAWTC 4 cut(s) 291, 734, 928, 985
Tru1I TTAA 5 cut(s) 318, 353, 627, 855, 1050
Tru9I TTAA 5 cut(s) 318, 353, 627, 855, 1050
TscAI CASTG 2 cut(s) 49, 679
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 5 cut(s) 39, 78, 81, 713, 1121
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 4 cut(s) 133, 399, 956, 984
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 2 cut(s) 49, 679
Van91I CCANNNNNTGG 1 cut(s) 791
VneI GTGCAC 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 283
VspI ATTAAT 1 cut(s) 855
XapI RAATTY 1 cut(s) 1082
XceI RCATGY 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 2 cut(s) 788, 977
Zsp2I ATGCAT 2 cut(s) 461, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.