Rw7G031120

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006935
Physical Location & Seq
Forward (+)
44320910 .. 44327383
6474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G031120.1

Sequence Viewer

Length: 1197 bp
ATGAAATTCGCAGTGAAGGCATGGAGCAGTGGAAGAGCACGGGCCGGGGTACTCCAGCTGGGCAGCTGCCCGAACCCAATAGAGACCCCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTGCCCCTTTTCATACCCCCTGACCTTCTCCCTTCTCTTCCTTCCCCCGACTCCCATCTCCTCCATCTTAGCTCCCTTCACTTCTTAGAAGGCATCTCACCTCAGACAATATCAAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCCGGACCACGGATTTGTAGCCATTCCGAGAGATTCTATTCAATGCCTCCCTGAATGTAATAGCACCAATAAATTGGGAGCATCTTTCGACACTCCCTGTGGTCGCAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTTTTCGTTAAAGCCTAATATATGGACCGCTTTGGCCGATGAAGTTCCTGCATGGGTGTCTGATAAGAGGAACAAGACCTCTGTTGATCGAACTGTAAGATGGCTCGACGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCTAGAACGTGAGCGATGTGCGCAAGAGGTGGCTAGTCGAAGTGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATTTTAGAAGAGGAGAAACCACGCTTGGTCAGTGGGCTGGGACTTCCAGAGGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATCTTTGACTCTACGTATATATACCATCTTACCGTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGAATGTCTCTGCTTTTCAGTTAACTAATATAGGAAGTGATCAAACAAAGATTAATTTGAGAGCAACAATTTACAGGAAAGACACTTCACCAATTGCTCAAAACTGTAAATGTTACACATGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATACATAATACTCACCACTATCTAAGTTTCTTCCGCTCAGTAAGGGAGGCAATTAAGTCTGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAAGCGTTATCATCTTGCTTCTGAAGCTGTTGTTGCCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

398

Amino Acids

43.76

Weight (kDa)

6.67

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 384 1.8e-87 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1020
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 2 cut(s) 527, 1020
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 1 cut(s) 1089
AciI CCGC 2 cut(s) 432, 1087
AclWI GGATC 4 cut(s) 549, 550, 562, 577
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 2 cut(s) 5, 1139
AcuI CTGAAG 1 cut(s) 1194
AfaI GTAC 2 cut(s) 51, 1001
AfiI CCNNNNNNNGG 2 cut(s) 272, 851
AgsI TTSAA 3 cut(s) 305, 398, 1052
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 6 cut(s) 58, 66, 189, 526, 1037, 1178
AluI AGCT 6 cut(s) 58, 66, 189, 526, 1037, 1178
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 77, 871
AlwI GGATC 4 cut(s) 549, 550, 562, 577
AoxI GGCC 3 cut(s) 42, 115, 438
ApeKI GCWGC 3 cut(s) 63, 66, 773
ApoI RAATTY 2 cut(s) 5, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 601, 675
AspS9I GGNCC 4 cut(s) 42, 115, 268, 429
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 4 cut(s) 207, 939, 1001, 1058
AsuNHI GCTAGC 1 cut(s) 1033
AvaII GGWCC 2 cut(s) 268, 429
BamHI GGATCC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 3 cut(s) 53, 75, 760
BccI CCATC 5 cut(s) 180, 189, 498, 819, 845
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 77, 871
BfaI CTAG 4 cut(s) 581, 612, 848, 1034
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 527, 1020
BisI GCNGC 3 cut(s) 64, 67, 774
BlsI GCNGC 3 cut(s) 65, 68, 775
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 268, 429
BmgT120I GGNCC 4 cut(s) 42, 115, 268, 429
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 1 cut(s) 46
BmsI GCATC 3 cut(s) 219, 243, 353
BmtI GCTAGC 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 851, 883
BpmI CTGGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 46
BsaAI YACGTR 1 cut(s) 801
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 45, 271
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 851
BseDI CCNNGG 2 cut(s) 45, 271
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 2 cut(s) 272, 851
BseMII CTCAG 4 cut(s) 233, 1052, 1104, 1137
BseRI GAGGAG 4 cut(s) 89, 167, 728, 767
BseXI GCAGC 3 cut(s) 53, 75, 760
BseYI CCCAGC 3 cut(s) 58, 256, 739
BshFI GGCC 3 cut(s) 44, 117, 440
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 2 cut(s) 45, 266
BslFI GGGAC 1 cut(s) 756
BslI CCNNNNNNNGG 2 cut(s) 272, 851
BsmAI GTCTC 2 cut(s) 77, 871
BsmFI GGGAC 1 cut(s) 756
BsmI GAATGC 2 cut(s) 519, 521
BsnI GGCC 3 cut(s) 44, 117, 440
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 5 cut(s) 490, 542, 554, 569, 898
BspACI CCGC 2 cut(s) 432, 1087
BspANI GGCC 3 cut(s) 44, 117, 440
BspCNI CTCAG 4 cut(s) 232, 1051, 1103, 1138
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 527, 1020
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 4 cut(s) 549, 550, 562, 577
BspQI GCTCTTC 2 cut(s) 28, 99
BspTNI GGTCTC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 1089
BssECI CCNNGG 2 cut(s) 45, 271
BssMI GATC 5 cut(s) 490, 542, 554, 569, 898
Bst4CI ACNGT 3 cut(s) 499, 820, 965
Bst6I CTCTTC 4 cut(s) 28, 99, 159, 705
BstAPI GCANNNNNTGC 1 cut(s) 253
BstBAI YACGTR 1 cut(s) 801
BstC8I GCNNGC 3 cut(s) 119, 1035, 1189
BstDEI CTNAG 7 cut(s) 185, 202, 219, 1038, 1076, 1090, 1146
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 1 cut(s) 673
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 601, 675
BstKTI GATC 5 cut(s) 493, 545, 557, 572, 901
BstMAI GTCTC 2 cut(s) 77, 871
BstMBI GATC 5 cut(s) 490, 542, 554, 569, 898
BstMWI GCNNNNNNNGC 6 cut(s) 17, 253, 262, 598, 1175, 1184
BstNSI RCATGY 2 cut(s) 981, 1195
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 2 cut(s) 384, 774
BstSNI TACGTA 1 cut(s) 801
BstV1I GCAGC 3 cut(s) 53, 75, 760
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 542, 554, 569
BsuRI GGCC 3 cut(s) 44, 117, 440
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 3 cut(s) 18, 34, 739
BveI ACCTGC 2 cut(s) 527, 1020
Cac8I GCNNGC 3 cut(s) 119, 1035, 1189
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 2 cut(s) 601, 675
Cfr13I GGNCC 4 cut(s) 42, 115, 268, 429
Csp6I GTAC 2 cut(s) 50, 1000
CviAII CATG 6 cut(s) 21, 456, 664, 978, 1025, 1192
CviQI GTAC 2 cut(s) 50, 1000
DdeI CTNAG 7 cut(s) 185, 202, 219, 1038, 1076, 1090, 1146
DpnI GATC 5 cut(s) 492, 544, 556, 571, 900
DpnII GATC 5 cut(s) 490, 542, 554, 569, 898
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 4 cut(s) 28, 99, 159, 705
EarI CTCTTC 4 cut(s) 28, 99, 159, 705
Eco105I TACGTA 1 cut(s) 801
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 2 cut(s) 268, 429
Eco57I CTGAAG 1 cut(s) 1194
EcoO109I RGGNCCY 1 cut(s) 115
EcoRV GATATC 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 521
FaeI CATG 6 cut(s) 24, 459, 667, 981, 1028, 1195
FaqI GGGAC 1 cut(s) 756
FatI CATG 6 cut(s) 20, 455, 663, 977, 1024, 1191
FbaI TGATCA 1 cut(s) 898
Fnu4HI GCNGC 3 cut(s) 64, 67, 774
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 3 cut(s) 64, 67, 774
FspBI CTAG 4 cut(s) 581, 612, 848, 1034
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 600, 674
GluI GCNGC 3 cut(s) 64, 67, 774
GsaI CCCAGC 3 cut(s) 62, 260, 743
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 3 cut(s) 44, 117, 440
HapII CCGG 2 cut(s) 45, 266
HhaI GCGC 2 cut(s) 601, 675
Hin1II CATG 6 cut(s) 24, 459, 667, 981, 1028, 1195
Hin6I GCGC 2 cut(s) 599, 673
HinP1I GCGC 2 cut(s) 599, 673
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HinfI GANTC 5 cut(s) 167, 296, 794, 985, 1042
HpaI GTTAAC 1 cut(s) 882
HpaII CCGG 2 cut(s) 45, 266
HphI GGTGA 4 cut(s) 207, 939, 1001, 1058
Hpy166II GTNNAC 1 cut(s) 882
Hpy188I TCNGA 7 cut(s) 222, 291, 466, 1041, 1133, 1147, 1174
Hpy188III TCNNGA 3 cut(s) 581, 749, 1025
Hpy8I GTNNAC 1 cut(s) 882
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 7 cut(s) 10, 152, 159, 168, 200, 203, 850
HpyCH4III ACNGT 3 cut(s) 499, 820, 965
HpyCH4IV ACGT 2 cut(s) 586, 800
HpyCH4V TGCA 7 cut(s) 247, 455, 519, 763, 776, 833, 1191
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 253, 262, 598, 1175, 1184
HpyF3I CTNAG 7 cut(s) 185, 202, 219, 1038, 1076, 1090, 1146
HpySE526I ACGT 2 cut(s) 586, 800
Hsp92II CATG 6 cut(s) 24, 459, 667, 981, 1028, 1195
HspAI GCGC 2 cut(s) 599, 673
Ksp22I TGATCA 1 cut(s) 898
KspAI GTTAAC 1 cut(s) 882
Kzo9I GATC 5 cut(s) 490, 542, 554, 569, 898
LguI GCTCTTC 2 cut(s) 28, 99
LmnI GCTCC 3 cut(s) 24, 194, 341
Lsp1109I GCAGC 3 cut(s) 53, 75, 760
LweI GCATC 3 cut(s) 219, 243, 353
MaeI CTAG 4 cut(s) 581, 612, 848, 1034
MaeII ACGT 2 cut(s) 586, 800
MaeIII GTNAC 2 cut(s) 694, 971
MalI GATC 5 cut(s) 492, 544, 556, 571, 900
MbiI CCGCTC 1 cut(s) 1089
MboI GATC 5 cut(s) 490, 542, 554, 569, 898
MboII GAAGA 6 cut(s) 45, 86, 146, 564, 722, 1075
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 1 cut(s) 40
MlyI GAGTC 2 cut(s) 161, 788
MmeI TCCRAC 2 cut(s) 544, 802
Mph1103I ATGCAT 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 6 cut(s) 378, 413, 687, 881, 912, 1107
MspA1I CMGCKG 2 cut(s) 58, 66
MspI CCGG 2 cut(s) 45, 266
MspR9I CCNGG 1 cut(s) 46
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 2 cut(s) 519, 521
MwoI GCNNNNNNNGC 6 cut(s) 17, 253, 262, 598, 1175, 1184
NciI CCSGG 1 cut(s) 46
NdeII GATC 5 cut(s) 490, 542, 554, 569, 898
NheI GCTAGC 1 cut(s) 1033
NlaIII CATG 6 cut(s) 24, 459, 667, 981, 1028, 1195
NlaIV GGNNCC 1 cut(s) 556
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 2 cut(s) 981, 1195
PagI TCATGA 1 cut(s) 1024
PaqCI CACCTGC 2 cut(s) 527, 1020
PciSI GCTCTTC 2 cut(s) 28, 99
PctI GAATGC 2 cut(s) 519, 521
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 3 cut(s) 296, 985, 1042
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 3 cut(s) 65, 68, 775
PleI GAGTC 2 cut(s) 161, 788
PpsI GAGTC 2 cut(s) 161, 788
Ppu21I YACGTR 1 cut(s) 801
PshBI ATTAAT 1 cut(s) 912
PspFI CCCAGC 3 cut(s) 58, 256, 739
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 4 cut(s) 42, 115, 268, 429
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 3 cut(s) 542, 554, 569
PvuII CAGCTG 2 cut(s) 58, 66
RsaI GTAC 2 cut(s) 51, 1001
RsaNI GTAC 2 cut(s) 50, 1000
SapI GCTCTTC 2 cut(s) 28, 99
SaqAI TTAA 6 cut(s) 378, 413, 687, 881, 912, 1107
SatI GCNGC 3 cut(s) 64, 67, 774
Sau3AI GATC 5 cut(s) 490, 542, 554, 569, 898
Sau96I GGNCC 4 cut(s) 42, 115, 268, 429
SchI GAGTC 2 cut(s) 161, 788
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 3 cut(s) 219, 243, 353
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 2 cut(s) 268, 429
SnaBI TACGTA 1 cut(s) 801
SsiI CCGC 2 cut(s) 432, 1087
SspMI CTAG 4 cut(s) 581, 612, 848, 1034
StyD4I CCNGG 1 cut(s) 44
TaaI ACNGT 3 cut(s) 499, 820, 965
TaiI ACGT 2 cut(s) 589, 803
TaqI TCGA 4 cut(s) 351, 493, 510, 616
TfiI GAWTC 3 cut(s) 296, 985, 1042
Tru1I TTAA 6 cut(s) 378, 413, 687, 881, 912, 1107
Tru9I TTAA 6 cut(s) 378, 413, 687, 881, 912, 1107
TscAI CASTG 3 cut(s) 18, 34, 739
TseI GCWGC 3 cut(s) 63, 66, 773
TspDTI ATGAA 5 cut(s) 17, 118, 459, 1013, 1041
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 3 cut(s) 18, 34, 739
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 268, 429
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 2 cut(s) 5, 1139
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 2 cut(s) 981, 1195
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 4 cut(s) 581, 612, 848, 1034
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.