Rh7CG435100

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006935
Physical Location & Seq
Reverse (-)
56964976 .. 56970235
5260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG435100.1

Sequence Viewer

Length: 681 bp
ATGCATTCAGCTAAATCGGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCACAAGAGGTGGCCAGCCGAAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATCTTAGAAGAGGAGAAACCACGCTTGGTCAGTCGGCTTGGACTTCCAGAAGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATTTTTGATTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATCTCTCTGCTTTTCAGTTGACTAATATAGGAAGTGATCAGACAAAGATTAATTTGAGAGCAACAATGCACAGGAAAGACACTTCACCAATTGTTGAAAATTGTAAATGTTACACGTGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATGCATAATACTCACCACTATCTAAGTTTCTTCCGCTCAGTATTGGAGGCAATTAAGTCTGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAAGCGTTATCCTCTTGCTTCTGAAGCTGCTGTTACCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

226

Amino Acids

25.05

Weight (kDa)

5.98

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 4 - 212 1e-59 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 504
Acc36I ACCTGC 1 cut(s) 504
AccB7I CCANNNNNTGG 1 cut(s) 332
AccBSI CCGCTC 1 cut(s) 573
AciI CCGC 1 cut(s) 571
AclWI GGATC 4 cut(s) 30, 31, 43, 58
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 1 cut(s) 678
AcvI CACGTG 1 cut(s) 462
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 3 cut(s) 62, 443, 536
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AloI GAACNNNNNNTCC 2 cut(s) 42, 74
AluBI AGCT 3 cut(s) 11, 521, 662
AluI AGCT 3 cut(s) 11, 521, 662
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 335
Alw44I GTGCAC 1 cut(s) 78
AlwI GGATC 4 cut(s) 30, 31, 43, 58
AoxI GGCC 1 cut(s) 90
ApaLI GTGCAC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 254, 662
ApoI RAATTY 1 cut(s) 623
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 1 cut(s) 156
AsuHPI GGTGA 3 cut(s) 423, 485, 542
AsuNHI GCTAGC 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 92
BamHI GGATCC 1 cut(s) 35
BbrPI CACGTG 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 2 cut(s) 241, 649
BccI CCATC 2 cut(s) 300, 326
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 2 cut(s) 329, 518
BfmI CTRYAG 1 cut(s) 255
BfoI RGCGCY 1 cut(s) 157
BfuAI ACCTGC 1 cut(s) 504
BisI GCNGC 2 cut(s) 255, 663
BlsI GCNGC 2 cut(s) 256, 664
BmiI GGNNCC 1 cut(s) 37
BmtI GCTAGC 1 cut(s) 521
BsaAI YACGTR 2 cut(s) 282, 462
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMII CTCAG 3 cut(s) 536, 588, 621
BseRI GAGGAG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 2 cut(s) 241, 649
BshFI GGCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 335
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 4 cut(s) 23, 35, 50, 382
BspACI CCGC 1 cut(s) 571
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 3 cut(s) 535, 587, 622
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 1 cut(s) 37
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 504
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 4 cut(s) 30, 31, 43, 58
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 4 cut(s) 23, 35, 50, 382
BssT1I CCWWGG 1 cut(s) 301
Bst6I CTCTTC 2 cut(s) 186, 228
BstBAI YACGTR 2 cut(s) 282, 462
BstC8I GCNNGC 2 cut(s) 94, 519
BstDEI CTNAG 5 cut(s) 187, 522, 560, 574, 630
BstF5I GGATG 1 cut(s) 154
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 4 cut(s) 26, 38, 53, 385
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 4 cut(s) 23, 35, 50, 382
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNSI RCATGY 1 cut(s) 679
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 1 cut(s) 82
BstSNI TACGTA 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 241, 649
BstX2I RGATCY 3 cut(s) 23, 35, 50
BstYI RGATCY 3 cut(s) 23, 35, 50
BsuRI GGCC 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 94, 519
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 156
Csp6I GTAC 1 cut(s) 484
CviAII CATG 3 cut(s) 145, 509, 676
CviJI RGCY 9 cut(s) 11, 92, 96, 111, 133, 220, 309, 521, 662
CviKI_1 RGCY 9 cut(s) 11, 92, 96, 111, 133, 220, 309, 521, 662
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 5 cut(s) 187, 522, 560, 574, 630
DpnI GATC 4 cut(s) 25, 37, 52, 384
DpnII GATC 4 cut(s) 23, 35, 50, 382
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 186, 228
EarI CTCTTC 2 cut(s) 186, 228
Eco105I TACGTA 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 301
Eco31I GGTCTC 1 cut(s) 335
Eco32I GATATC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 678
Eco72I CACGTG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 2 cut(s) 6, 543
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 3 cut(s) 148, 512, 679
FaiI YATR 9 cut(s) 146, 285, 287, 289, 374, 474, 510, 543, 677
FatI CATG 3 cut(s) 144, 508, 675
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 255, 663
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 2 cut(s) 255, 663
FspBI CTAG 2 cut(s) 329, 518
GlaI GCGC 1 cut(s) 155
GluI GCNGC 2 cut(s) 255, 663
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 1 cut(s) 92
HhaI GCGC 1 cut(s) 156
Hin1II CATG 3 cut(s) 148, 512, 679
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HinfI GANTC 3 cut(s) 275, 469, 526
HphI GGTGA 3 cut(s) 423, 485, 542
Hpy166II GTNNAC 2 cut(s) 80, 366
Hpy188I TCNGA 5 cut(s) 387, 525, 617, 631, 658
Hpy188III TCNNGA 3 cut(s) 62, 230, 509
Hpy8I GTNNAC 2 cut(s) 80, 366
HpyAV CCTTC 1 cut(s) 331
HpyCH4IV ACGT 3 cut(s) 67, 281, 461
HpyCH4V TGCA 8 cut(s) 4, 80, 244, 257, 314, 415, 541, 675
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 5 cut(s) 187, 522, 560, 574, 630
HpySE526I ACGT 3 cut(s) 67, 281, 461
Hsp92II CATG 3 cut(s) 148, 512, 679
HspAI GCGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 4 cut(s) 23, 35, 50, 382
Lsp1109I GCAGC 2 cut(s) 241, 649
MaeI CTAG 2 cut(s) 329, 518
MaeII ACGT 3 cut(s) 67, 281, 461
MaeIII GTNAC 3 cut(s) 175, 455, 667
MalI GATC 4 cut(s) 25, 37, 52, 384
MbiI CCGCTC 1 cut(s) 573
MboI GATC 4 cut(s) 23, 35, 50, 382
MboII GAAGA 4 cut(s) 45, 203, 245, 559
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 3 cut(s) 23, 35, 50
MhlI GDGCHC 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 8 cut(s) 261, 397, 435, 445, 588, 601, 611, 623
MluNI TGGCCA 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 25
MnlI CCTC 8 cut(s) 41, 79, 116, 187, 229, 299, 577, 657
Mox20I TGGCCA 1 cut(s) 92
Mph1103I ATGCAT 2 cut(s) 6, 543
MscI TGGCCA 1 cut(s) 92
MseI TTAA 3 cut(s) 168, 396, 591
Msp20I TGGCCA 1 cut(s) 92
MunI CAATTG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 4
MwoI GCNNNNNNNGC 1 cut(s) 659
NdeII GATC 4 cut(s) 23, 35, 50, 382
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 3 cut(s) 148, 512, 679
NlaIV GGNNCC 1 cut(s) 37
NsiI ATGCAT 2 cut(s) 6, 543
NspI RCATGY 1 cut(s) 679
PagI TCATGA 1 cut(s) 508
PaqCI CACCTGC 1 cut(s) 504
PctI GAATGC 1 cut(s) 4
PfeI GAWTC 3 cut(s) 275, 469, 526
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 2 cut(s) 256, 664
PmaCI CACGTG 1 cut(s) 462
PmlI CACGTG 1 cut(s) 462
Ppu21I YACGTR 2 cut(s) 282, 462
PshBI ATTAAT 1 cut(s) 396
PspCI CACGTG 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 3 cut(s) 23, 35, 50
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
SaqAI TTAA 3 cut(s) 168, 396, 591
SatI GCNGC 2 cut(s) 255, 663
Sau3AI GATC 4 cut(s) 23, 35, 50, 382
SduI GDGCHC 1 cut(s) 82
SfcI CTRYAG 1 cut(s) 255
SnaBI TACGTA 1 cut(s) 282
Sse9I AATT 8 cut(s) 261, 397, 435, 445, 588, 601, 611, 623
SsiI CCGC 1 cut(s) 571
SspMI CTAG 2 cut(s) 329, 518
StyI CCWWGG 1 cut(s) 301
TaiI ACGT 3 cut(s) 70, 284, 464
TasI AATT 8 cut(s) 261, 397, 435, 445, 588, 601, 611, 623
TfiI GAWTC 3 cut(s) 275, 469, 526
Tru1I TTAA 3 cut(s) 168, 396, 591
Tru9I TTAA 3 cut(s) 168, 396, 591
TseI GCWGC 2 cut(s) 254, 662
TspDTI ATGAA 2 cut(s) 497, 525
Van91I CCANNNNNTGG 1 cut(s) 332
VneI GTGCAC 1 cut(s) 78
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 1 cut(s) 623
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XspI CTAG 2 cut(s) 329, 518
Zsp2I ATGCAT 2 cut(s) 6, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.