Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g32910 FvH4_5g32910 |
| malus_domestica | MD15G1399400.v1.1 |
| prunus_persica | Prupe.1G546900_v2.0.a1 Prupe.1G546900_v2.0.a1 Prupe.1G546900_v2.0.a1 Prupe.1G546900_v2.0.a1 |
| pyrus_communis | pycom15g35840 |
| rosa_chinensis | RchiOBHm_Chr7g0224381 RchiOBHm_Chr7g0230881 |
| rosa_laevigata | RLG00000001455 RLG00000002175 |
| rosa_multiflora | Rmu_sc0000483.1_g000007 Rmu_sc0000551.1_g000016 Rmu_sc0006366.1_g000006 |
| rosa_roxburghii | Rroxscaffold_3G00238700 |
| rosa_rugosa | Rorug07G0201000 Rorug07G0262800 |
| rosa_samantha | Rh7AG380700 Rh7AG416100 Rh7BG330800 Rh7BG392100 Rh7CG400100 Rh7CG435100 Rh7DG410600 |
| rosa_wichuraiana | Rw7G031120 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 828 |
| Acc36I | ACCTGC | 1 cut(s) | 828 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 656 |
| AciI | CCGC | 2 cut(s) | 13, 895 |
| AclWI | GGATC | 5 cut(s) | 257, 258, 270, 285, 499 |
| AcoI | YGGCCR | 1 cut(s) | 317 |
| AcsI | RAATTY | 1 cut(s) | 947 |
| AcuI | CTGAAG | 1 cut(s) | 1002 |
| AcvI | CACGTG | 1 cut(s) | 786 |
| AfaI | GTAC | 2 cut(s) | 76, 809 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 656 |
| AflIII | ACRYGT | 1 cut(s) | 783 |
| AgsI | TTSAA | 5 cut(s) | 53, 146, 289, 767, 860 |
| AjnI | CCWGG | 1 cut(s) | 533 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 269, 301 |
| AluBI | AGCT | 2 cut(s) | 845, 986 |
| AluI | AGCT | 2 cut(s) | 845, 986 |
| Alw21I | GWGCWC | 1 cut(s) | 309 |
| Alw26I | GTCTC | 3 cut(s) | 95, 511, 659 |
| Alw44I | GTGCAC | 1 cut(s) | 305 |
| AlwI | GGATC | 5 cut(s) | 257, 258, 270, 285, 499 |
| AoxI | GGCC | 2 cut(s) | 164, 317 |
| ApaLI | GTGCAC | 1 cut(s) | 305 |
| ApeKI | GCWGC | 2 cut(s) | 578, 986 |
| ApoI | RAATTY | 1 cut(s) | 947 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 8, 40 |
| AseI | ATTAAT | 1 cut(s) | 720 |
| AspLEI | GCGC | 1 cut(s) | 423 |
| AspS9I | GGNCC | 1 cut(s) | 16 |
| AsuHPI | GGTGA | 3 cut(s) | 747, 809, 866 |
| AsuNHI | GCTAGC | 1 cut(s) | 841 |
| AvaII | GGWCC | 1 cut(s) | 16 |
| BaeGI | GKGCMC | 1 cut(s) | 309 |
| BalI | TGGCCA | 1 cut(s) | 319 |
| BamHI | GGATCC | 1 cut(s) | 262 |
| BbrPI | CACGTG | 1 cut(s) | 786 |
| Bbv12I | GWGCWC | 1 cut(s) | 309 |
| BbvI | GCAGC | 2 cut(s) | 565, 973 |
| BccI | CCATC | 3 cut(s) | 469, 624, 650 |
| BceAI | ACGGC | 1 cut(s) | 559 |
| BciT130I | CCWGG | 1 cut(s) | 535 |
| BclI | TGATCA | 1 cut(s) | 706 |
| BcoDI | GTCTC | 3 cut(s) | 95, 511, 659 |
| BfaI | CTAG | 2 cut(s) | 653, 842 |
| BfmI | CTRYAG | 3 cut(s) | 132, 343, 579 |
| BfoI | RGCGCY | 1 cut(s) | 424 |
| BfuAI | ACCTGC | 1 cut(s) | 828 |
| BisI | GCNGC | 2 cut(s) | 579, 987 |
| BlsI | GCNGC | 2 cut(s) | 580, 988 |
| Bme1390I | CCNGG | 1 cut(s) | 535 |
| Bme18I | GGWCC | 1 cut(s) | 16 |
| BmgT120I | GGNCC | 1 cut(s) | 16 |
| BmiI | GGNNCC | 2 cut(s) | 17, 264 |
| BmrFI | CCNGG | 1 cut(s) | 535 |
| BmsI | GCATC | 2 cut(s) | 101, 365 |
| BmtI | GCTAGC | 1 cut(s) | 845 |
| BsaAI | YACGTR | 2 cut(s) | 606, 786 |
| BsaI | GGTCTC | 1 cut(s) | 659 |
| BsaJI | CCNNGG | 2 cut(s) | 19, 231 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 464, 494 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 656 |
| BseBI | CCWGG | 1 cut(s) | 535 |
| BseDI | CCNNGG | 2 cut(s) | 19, 231 |
| BseGI | GGATG | 1 cut(s) | 421 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 656 |
| BseMII | CTCAG | 2 cut(s) | 860, 945 |
| BseSI | GKGCMC | 1 cut(s) | 309 |
| BseXI | GCAGC | 2 cut(s) | 565, 973 |
| BseYI | CCCAGC | 2 cut(s) | 4, 508 |
| BshFI | GGCC | 2 cut(s) | 166, 319 |
| BsiHKAI | GWGCWC | 1 cut(s) | 309 |
| BslFI | GGGAC | 1 cut(s) | 29 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 656 |
| BsmAI | GTCTC | 3 cut(s) | 95, 511, 659 |
| BsmFI | GGGAC | 1 cut(s) | 29 |
| BsnI | GGCC | 2 cut(s) | 166, 319 |
| Bso31I | GGTCTC | 1 cut(s) | 659 |
| Bsp1286I | GDGCHC | 1 cut(s) | 309 |
| Bsp143I | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| BspACI | CCGC | 2 cut(s) | 13, 895 |
| BspANI | GGCC | 2 cut(s) | 166, 319 |
| BspCNI | CTCAG | 2 cut(s) | 859, 946 |
| BspHI | TCATGA | 1 cut(s) | 832 |
| BspLI | GGNNCC | 2 cut(s) | 17, 264 |
| BspMAI | CTGCAG | 1 cut(s) | 583 |
| BspMI | ACCTGC | 1 cut(s) | 828 |
| BspOI | GCTAGC | 1 cut(s) | 845 |
| BspPI | GGATC | 5 cut(s) | 257, 258, 270, 285, 499 |
| BspTNI | GGTCTC | 1 cut(s) | 659 |
| BssECI | CCNNGG | 2 cut(s) | 19, 231 |
| BssMI | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| Bst2UI | CCWGG | 1 cut(s) | 535 |
| Bst4CI | ACNGT | 3 cut(s) | 247, 347, 625 |
| Bst6I | CTCTTC | 1 cut(s) | 552 |
| BstBAI | YACGTR | 2 cut(s) | 606, 786 |
| BstC8I | GCNNGC | 3 cut(s) | 321, 843, 997 |
| BstDEI | CTNAG | 4 cut(s) | 846, 884, 898, 954 |
| BstDSI | CCRYGG | 1 cut(s) | 19 |
| BstF5I | GGATG | 1 cut(s) | 421 |
| BstH2I | RGCGCY | 1 cut(s) | 424 |
| BstHHI | GCGC | 1 cut(s) | 423 |
| BstKTI | GATC | 6 cut(s) | 241, 253, 265, 280, 507, 709 |
| BstMAI | GTCTC | 3 cut(s) | 95, 511, 659 |
| BstMBI | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 10, 983, 992 |
| BstNI | CCWGG | 1 cut(s) | 535 |
| BstNSI | RCATGY | 1 cut(s) | 1003 |
| BstSCI | CCNGG | 1 cut(s) | 533 |
| BstSFI | CTRYAG | 3 cut(s) | 132, 343, 579 |
| BstSLI | GKGCMC | 1 cut(s) | 309 |
| BstSNI | TACGTA | 1 cut(s) | 606 |
| BstV1I | GCAGC | 2 cut(s) | 565, 973 |
| BstX2I | RGATCY | 4 cut(s) | 250, 262, 277, 504 |
| BstYI | RGATCY | 4 cut(s) | 250, 262, 277, 504 |
| BsuRI | GGCC | 2 cut(s) | 166, 319 |
| BtgI | CCRYGG | 1 cut(s) | 19 |
| BtgZI | GCGATG | 1 cut(s) | 315 |
| BtsCI | GGATG | 1 cut(s) | 421 |
| BtsIMutI | CAGTG | 1 cut(s) | 252 |
| BveI | ACCTGC | 1 cut(s) | 828 |
| Cac8I | GCNNGC | 3 cut(s) | 321, 843, 997 |
| CciI | TCATGA | 1 cut(s) | 832 |
| CfoI | GCGC | 1 cut(s) | 423 |
| Cfr13I | GGNCC | 1 cut(s) | 16 |
| Csp6I | GTAC | 2 cut(s) | 75, 808 |
| CviAII | CATG | 4 cut(s) | 204, 412, 833, 1000 |
| CviQI | GTAC | 2 cut(s) | 75, 808 |
| DdeI | CTNAG | 4 cut(s) | 846, 884, 898, 954 |
| DpnI | GATC | 6 cut(s) | 240, 252, 264, 279, 506, 708 |
| DpnII | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| EaeI | YGGCCR | 1 cut(s) | 317 |
| Eam1104I | CTCTTC | 1 cut(s) | 552 |
| EarI | CTCTTC | 1 cut(s) | 552 |
| Eco105I | TACGTA | 1 cut(s) | 606 |
| Eco147I | AGGCCT | 1 cut(s) | 166 |
| Eco31I | GGTCTC | 1 cut(s) | 659 |
| Eco32I | GATATC | 1 cut(s) | 592 |
| Eco47I | GGWCC | 1 cut(s) | 16 |
| Eco57I | CTGAAG | 1 cut(s) | 1002 |
| Eco72I | CACGTG | 1 cut(s) | 786 |
| EcoRII | CCWGG | 1 cut(s) | 533 |
| EcoRV | GATATC | 1 cut(s) | 592 |
| EcoT22I | ATGCAT | 1 cut(s) | 867 |
| FaeI | CATG | 4 cut(s) | 207, 415, 836, 1003 |
| FaqI | GGGAC | 1 cut(s) | 29 |
| FatI | CATG | 4 cut(s) | 203, 411, 832, 999 |
| FauI | CCCGC | 1 cut(s) | 6 |
| FbaI | TGATCA | 1 cut(s) | 706 |
| Fnu4HI | GCNGC | 2 cut(s) | 579, 987 |
| FokI | GGATG | 1 cut(s) | 428 |
| Fsp4HI | GCNGC | 2 cut(s) | 579, 987 |
| FspBI | CTAG | 2 cut(s) | 653, 842 |
| GlaI | GCGC | 1 cut(s) | 422 |
| GluI | GCNGC | 2 cut(s) | 579, 987 |
| GsaI | CCCAGC | 2 cut(s) | 8, 512 |
| HaeII | RGCGCY | 1 cut(s) | 424 |
| HaeIII | GGCC | 2 cut(s) | 166, 319 |
| HhaI | GCGC | 1 cut(s) | 423 |
| Hin1II | CATG | 4 cut(s) | 207, 415, 836, 1003 |
| Hin6I | GCGC | 1 cut(s) | 421 |
| HinP1I | GCGC | 1 cut(s) | 421 |
| HincII | GTYRAC | 2 cut(s) | 340, 690 |
| HindII | GTYRAC | 2 cut(s) | 340, 690 |
| HinfI | GANTC | 7 cut(s) | 24, 44, 103, 496, 599, 793, 850 |
| HpaI | GTTAAC | 1 cut(s) | 340 |
| HphI | GGTGA | 3 cut(s) | 747, 809, 866 |
| Hpy166II | GTNNAC | 3 cut(s) | 307, 340, 690 |
| Hpy188III | TCNNGA | 3 cut(s) | 100, 289, 833 |
| Hpy8I | GTNNAC | 3 cut(s) | 307, 340, 690 |
| HpyAV | CCTTC | 1 cut(s) | 655 |
| HpyCH4III | ACNGT | 3 cut(s) | 247, 347, 625 |
| HpyCH4IV | ACGT | 3 cut(s) | 294, 605, 785 |
| HpyCH4V | TGCA | 9 cut(s) | 92, 203, 307, 568, 581, 638, 739, 865, 999 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 10, 983, 992 |
| HpyF3I | CTNAG | 4 cut(s) | 846, 884, 898, 954 |
| HpySE526I | ACGT | 3 cut(s) | 294, 605, 785 |
| Hsp92II | CATG | 4 cut(s) | 207, 415, 836, 1003 |
| HspAI | GCGC | 1 cut(s) | 421 |
| Ksp22I | TGATCA | 1 cut(s) | 706 |
| KspAI | GTTAAC | 1 cut(s) | 340 |
| Kzo9I | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| Lsp1109I | GCAGC | 2 cut(s) | 565, 973 |
| LweI | GCATC | 2 cut(s) | 101, 365 |
| MaeI | CTAG | 2 cut(s) | 653, 842 |
| MaeII | ACGT | 3 cut(s) | 294, 605, 785 |
| MaeIII | GTNAC | 2 cut(s) | 442, 779 |
| MalI | GATC | 6 cut(s) | 240, 252, 264, 279, 506, 708 |
| MboI | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| MboII | GAAGA | 6 cut(s) | 147, 272, 354, 514, 569, 883 |
| MfeI | CAATTG | 1 cut(s) | 759 |
| MflI | RGATCY | 4 cut(s) | 250, 262, 277, 504 |
| MhlI | GDGCHC | 1 cut(s) | 309 |
| MlsI | TGGCCA | 1 cut(s) | 319 |
| MluNI | TGGCCA | 1 cut(s) | 319 |
| MlyI | GAGTC | 3 cut(s) | 97, 505, 593 |
| MmeI | TCCRAC | 3 cut(s) | 252, 479, 607 |
| Mox20I | TGGCCA | 1 cut(s) | 319 |
| Mph1103I | ATGCAT | 1 cut(s) | 867 |
| MscI | TGGCCA | 1 cut(s) | 319 |
| MseI | TTAA | 7 cut(s) | 126, 161, 339, 435, 527, 720, 915 |
| Msp20I | TGGCCA | 1 cut(s) | 319 |
| MspR9I | CCNGG | 1 cut(s) | 535 |
| MunI | CAATTG | 1 cut(s) | 759 |
| MvaI | CCWGG | 1 cut(s) | 535 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 10, 983, 992 |
| NdeII | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| NheI | GCTAGC | 1 cut(s) | 841 |
| NlaIII | CATG | 4 cut(s) | 207, 415, 836, 1003 |
| NlaIV | GGNNCC | 2 cut(s) | 17, 264 |
| NsiI | ATGCAT | 1 cut(s) | 867 |
| NspI | RCATGY | 1 cut(s) | 1003 |
| PagI | TCATGA | 1 cut(s) | 832 |
| PaqCI | CACCTGC | 1 cut(s) | 828 |
| PceI | AGGCCT | 1 cut(s) | 166 |
| PfeI | GAWTC | 4 cut(s) | 24, 44, 793, 850 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 656 |
| PkrI | GCNGC | 2 cut(s) | 580, 988 |
| PleI | GAGTC | 3 cut(s) | 97, 504, 593 |
| PmaCI | CACGTG | 1 cut(s) | 786 |
| PmlI | CACGTG | 1 cut(s) | 786 |
| PpsI | GAGTC | 3 cut(s) | 97, 504, 593 |
| Ppu21I | YACGTR | 2 cut(s) | 606, 786 |
| PshBI | ATTAAT | 1 cut(s) | 720 |
| Psp6I | CCWGG | 1 cut(s) | 533 |
| PspCI | CACGTG | 1 cut(s) | 786 |
| PspFI | CCCAGC | 2 cut(s) | 4, 508 |
| PspGI | CCWGG | 1 cut(s) | 533 |
| PspN4I | GGNNCC | 2 cut(s) | 17, 264 |
| PspPI | GGNCC | 1 cut(s) | 16 |
| PstI | CTGCAG | 1 cut(s) | 583 |
| PsuI | RGATCY | 4 cut(s) | 250, 262, 277, 504 |
| RsaI | GTAC | 2 cut(s) | 76, 809 |
| RsaNI | GTAC | 2 cut(s) | 75, 808 |
| SaqAI | TTAA | 7 cut(s) | 126, 161, 339, 435, 527, 720, 915 |
| SatI | GCNGC | 2 cut(s) | 579, 987 |
| Sau3AI | GATC | 6 cut(s) | 238, 250, 262, 277, 504, 706 |
| Sau96I | GGNCC | 1 cut(s) | 16 |
| SchI | GAGTC | 3 cut(s) | 97, 505, 593 |
| ScrFI | CCNGG | 1 cut(s) | 535 |
| SduI | GDGCHC | 1 cut(s) | 309 |
| SfaNI | GCATC | 2 cut(s) | 101, 365 |
| SfcI | CTRYAG | 3 cut(s) | 132, 343, 579 |
| SinI | GGWCC | 1 cut(s) | 16 |
| SnaBI | TACGTA | 1 cut(s) | 606 |
| SseBI | AGGCCT | 1 cut(s) | 166 |
| SsiI | CCGC | 2 cut(s) | 13, 895 |
| SspMI | CTAG | 2 cut(s) | 653, 842 |
| StuI | AGGCCT | 1 cut(s) | 166 |
| StyD4I | CCNGG | 1 cut(s) | 533 |
| TaaI | ACNGT | 3 cut(s) | 247, 347, 625 |
| TaiI | ACGT | 3 cut(s) | 297, 608, 788 |
| TaqI | TCGA | 2 cut(s) | 99, 241 |
| TfiI | GAWTC | 4 cut(s) | 24, 44, 793, 850 |
| Tru1I | TTAA | 7 cut(s) | 126, 161, 339, 435, 527, 720, 915 |
| Tru9I | TTAA | 7 cut(s) | 126, 161, 339, 435, 527, 720, 915 |
| TscAI | CASTG | 1 cut(s) | 252 |
| TseI | GCWGC | 2 cut(s) | 578, 986 |
| TspDTI | ATGAA | 3 cut(s) | 207, 821, 849 |
| TspGWI | ACGGA | 1 cut(s) | 36 |
| TspRI | CASTG | 1 cut(s) | 252 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 656 |
| VneI | GTGCAC | 1 cut(s) | 305 |
| VpaK11BI | GGWCC | 1 cut(s) | 16 |
| VspI | ATTAAT | 1 cut(s) | 720 |
| XapI | RAATTY | 1 cut(s) | 947 |
| XceI | RCATGY | 1 cut(s) | 1003 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 623 |
| XspI | CTAG | 2 cut(s) | 653, 842 |
| Zsp2I | ATGCAT | 1 cut(s) | 867 |