Rh7BG392100

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006935
Physical Location & Seq
Reverse (-)
43192325 .. 43198604
6280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG392100.1

Sequence Viewer

Length: 1203 bp
ATGAAATTCGCAGTGAAGGCATGGAGCAGTGGAAGAGCACGCGCAGGGGTACTCCAGCTCGGCAGCTGCCCGAACCCAATAGAGACCCCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTGCCCCTTTTCATACCCCTTGACCTTCTCCCTTCTCTTCCTTCCCCTGACTCCCATCTCCTCCATGTTAGCTCCCTTCACTTCTTAGAAGGCATCTCACCTCAGACAATATCGAAAATAGGAGGATTGCATAAGATGTTGGGTTTGCCGGACCACGGATTCGTAGCCATTCCGAGAGATTCTGTTCAATGCCTCTCAGAATGTAATAGTACCAATAAATTGGGAGCATCTTTCGAGACTCCCTGTGGTCGTAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTCTTCGTTAAAGCCTAATATATGGACCGCTTTGGCGGATGAAGTCCCTGCATGGGTGTCTGATAAGAGGAACAAGACGTCGGTTGATCGAACTGTAAGATGGCTTGATGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCGCAAGAGGTGGCCAGCCGAAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATCTTAGAAGAGGAGGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAAGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATTTTTGATTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATCTCTCTGCTTTTCAGTTAACTAATATAGGAAGTGATCAGACAAAGATTAATTTGAGAGCAACAATGTACAGGAAAGACACTTCACCAATTGTTGAAAATTGTAAATGTTACACGTGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATGCATAATACTCACCACTATCTAAGTTTCTTCCGCTTGGTAAGGGAGGCAATTAAGTCCGGTAATTTTGAGCAATTCAGACTTAAATTTTCTGAGAGTAAGCGTTATCATCTTGCTTCTGAAGCTGCTGTTGCCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

400

Amino Acids

44.05

Weight (kDa)

6.36

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 386 2.2e-87 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1026
AatII GACGTC 1 cut(s) 485
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 2 cut(s) 527, 1026
AccB7I CCANNNNNTGG 1 cut(s) 854
AccII CGCG 1 cut(s) 42
AciI CCGC 3 cut(s) 432, 440, 1093
AclWI GGATC 4 cut(s) 549, 550, 562, 577
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 2 cut(s) 5, 1145
AcuI CTGAAG 1 cut(s) 1200
AcvI CACGTG 1 cut(s) 984
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 4 cut(s) 51, 328, 938, 1007
AfiI CCNNNNNNNGG 3 cut(s) 272, 457, 854
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 5 cut(s) 305, 398, 581, 965, 1058
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 6 cut(s) 58, 66, 189, 526, 1043, 1184
AluI AGCT 6 cut(s) 58, 66, 189, 526, 1043, 1184
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 3 cut(s) 77, 347, 857
AlwI GGATC 4 cut(s) 549, 550, 562, 577
AoxI GGCC 2 cut(s) 115, 609
ApeKI GCWGC 4 cut(s) 63, 66, 776, 1184
ApoI RAATTY 2 cut(s) 5, 1145
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 1 cut(s) 918
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 3 cut(s) 44, 601, 675
AspS9I GGNCC 3 cut(s) 115, 268, 429
AsuHPI GGTGA 4 cut(s) 207, 945, 1007, 1064
AsuNHI GCTAGC 1 cut(s) 1039
AvaII GGWCC 2 cut(s) 268, 429
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 554
BbrPI CACGTG 1 cut(s) 984
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 4 cut(s) 53, 75, 763, 1171
BccI CCATC 4 cut(s) 180, 498, 822, 848
BclI TGATCA 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 77, 347, 857
BfaI CTAG 2 cut(s) 851, 1040
BfmI CTRYAG 2 cut(s) 384, 777
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 527, 1026
BisI GCNGC 4 cut(s) 64, 67, 777, 1185
BlsI GCNGC 4 cut(s) 65, 68, 778, 1186
Bme18I GGWCC 2 cut(s) 268, 429
BmgT120I GGNCC 3 cut(s) 115, 268, 429
BmiI GGNNCC 1 cut(s) 556
BmsI GCATC 2 cut(s) 219, 353
BmtI GCTAGC 1 cut(s) 1043
BpmI CTGGAG 1 cut(s) 38
BsaAI YACGTR 2 cut(s) 804, 984
BsaHI GRCGYC 1 cut(s) 482
BsaI GGTCTC 2 cut(s) 77, 857
BsaJI CCNNGG 2 cut(s) 271, 823
BsaWI WCCGGW 1 cut(s) 1118
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 457, 854
BseDI CCNNGG 2 cut(s) 271, 823
BseGI GGATG 2 cut(s) 448, 673
BseLI CCNNNNNNNGG 3 cut(s) 272, 457, 854
BseMII CTCAG 4 cut(s) 233, 327, 1058, 1143
BseRI GAGGAG 4 cut(s) 89, 167, 728, 731
BseXI GCAGC 4 cut(s) 53, 75, 763, 1171
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 2 cut(s) 117, 611
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 2 cut(s) 266, 1119
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 3 cut(s) 272, 457, 854
BsmAI GTCTC 3 cut(s) 77, 347, 857
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 2 cut(s) 519, 521
BsnI GGCC 2 cut(s) 117, 611
Bso31I GGTCTC 2 cut(s) 77, 857
Bsp1286I GDGCHC 1 cut(s) 40
Bsp1407I TGTACA 1 cut(s) 936
Bsp143I GATC 5 cut(s) 490, 542, 554, 569, 904
BspACI CCGC 3 cut(s) 432, 440, 1093
BspANI GGCC 2 cut(s) 117, 611
BspCNI CTCAG 4 cut(s) 232, 326, 1057, 1144
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 781
BspMI ACCTGC 2 cut(s) 527, 1026
BspOI GCTAGC 1 cut(s) 1043
BspPI GGATC 4 cut(s) 549, 550, 562, 577
BspQI GCTCTTC 2 cut(s) 28, 99
BspTNI GGTCTC 2 cut(s) 77, 857
BsrGI TGTACA 1 cut(s) 936
BssECI CCNNGG 2 cut(s) 271, 823
BssMI GATC 5 cut(s) 490, 542, 554, 569, 904
BssNI GRCGYC 1 cut(s) 482
BssT1I CCWWGG 1 cut(s) 823
Bst4CI ACNGT 1 cut(s) 499
Bst6I CTCTTC 5 cut(s) 28, 99, 159, 705, 750
BstACI GRCGYC 1 cut(s) 482
BstAUI TGTACA 1 cut(s) 936
BstBAI YACGTR 2 cut(s) 804, 984
BstC8I GCNNGC 5 cut(s) 40, 119, 613, 1041, 1195
BstDEI CTNAG 7 cut(s) 202, 219, 313, 706, 1044, 1082, 1152
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 448, 673
BstFNI CGCG 1 cut(s) 42
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 3 cut(s) 44, 601, 675
BstKTI GATC 5 cut(s) 493, 545, 557, 572, 907
BstMAI GTCTC 3 cut(s) 77, 347, 857
BstMBI GATC 5 cut(s) 490, 542, 554, 569, 904
BstMWI GCNNNNNNNGC 4 cut(s) 17, 598, 1181, 1190
BstNSI RCATGY 1 cut(s) 1201
BstSFI CTRYAG 2 cut(s) 384, 777
BstSNI TACGTA 1 cut(s) 804
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 4 cut(s) 53, 75, 763, 1171
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 542, 554, 569
BsuRI GGCC 2 cut(s) 117, 611
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 448, 673
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 3 cut(s) 18, 34, 742
BveI ACCTGC 2 cut(s) 527, 1026
Cac8I GCNNGC 5 cut(s) 40, 119, 613, 1041, 1195
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 3 cut(s) 44, 601, 675
Cfr13I GGNCC 3 cut(s) 115, 268, 429
Csp6I GTAC 4 cut(s) 50, 327, 937, 1006
CviAII CATG 6 cut(s) 21, 182, 456, 664, 1031, 1198
CviQI GTAC 4 cut(s) 50, 327, 937, 1006
DdeI CTNAG 7 cut(s) 202, 219, 313, 706, 1044, 1082, 1152
DpnI GATC 5 cut(s) 492, 544, 556, 571, 906
DpnII GATC 5 cut(s) 490, 542, 554, 569, 904
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 5 cut(s) 28, 99, 159, 705, 750
EarI CTCTTC 5 cut(s) 28, 99, 159, 705, 750
EciI GGCGGA 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 804
Eco130I CCWWGG 1 cut(s) 823
Eco31I GGTCTC 2 cut(s) 77, 857
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 268, 429
Eco57I CTGAAG 1 cut(s) 1200
Eco72I CACGTG 1 cut(s) 984
EcoO109I RGGNCCY 1 cut(s) 115
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 823
EcoT22I ATGCAT 2 cut(s) 521, 1065
ErhI CCWWGG 1 cut(s) 823
FaeI CATG 6 cut(s) 24, 185, 459, 667, 1034, 1201
FaqI GGGAC 1 cut(s) 434
FatI CATG 6 cut(s) 20, 181, 455, 663, 1030, 1197
FbaI TGATCA 1 cut(s) 904
Fnu4HI GCNGC 4 cut(s) 64, 67, 777, 1185
FokI GGATG 2 cut(s) 455, 680
Fsp4HI GCNGC 4 cut(s) 64, 67, 777, 1185
FspBI CTAG 2 cut(s) 851, 1040
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 3 cut(s) 43, 600, 674
GluI GCNGC 4 cut(s) 64, 67, 777, 1185
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 2 cut(s) 117, 611
HapII CCGG 2 cut(s) 266, 1119
HhaI GCGC 3 cut(s) 44, 601, 675
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 6 cut(s) 24, 185, 459, 667, 1034, 1201
Hin6I GCGC 3 cut(s) 42, 599, 673
HinP1I GCGC 3 cut(s) 42, 599, 673
HincII GTYRAC 1 cut(s) 888
HindII GTYRAC 1 cut(s) 888
HinfI GANTC 7 cut(s) 167, 276, 296, 355, 797, 991, 1048
HpaI GTTAAC 1 cut(s) 888
HpaII CCGG 2 cut(s) 266, 1119
HphI GGTGA 4 cut(s) 207, 945, 1007, 1064
Hpy166II GTNNAC 1 cut(s) 888
Hpy188I TCNGA 9 cut(s) 222, 291, 316, 466, 909, 1047, 1139, 1153, 1180
Hpy188III TCNNGA 4 cut(s) 352, 581, 752, 1031
Hpy8I GTNNAC 1 cut(s) 888
Hpy99I CGWCG 1 cut(s) 487
HpyAV CCTTC 7 cut(s) 10, 152, 159, 168, 200, 203, 853
HpyCH4III ACNGT 1 cut(s) 499
HpyCH4IV ACGT 4 cut(s) 482, 586, 803, 983
HpyCH4V TGCA 8 cut(s) 247, 455, 519, 766, 779, 836, 1063, 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 598, 1181, 1190
HpyF3I CTNAG 7 cut(s) 202, 219, 313, 706, 1044, 1082, 1152
HpySE526I ACGT 4 cut(s) 482, 586, 803, 983
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 6 cut(s) 24, 185, 459, 667, 1034, 1201
HspAI GCGC 3 cut(s) 42, 599, 673
Ksp22I TGATCA 1 cut(s) 904
KspAI GTTAAC 1 cut(s) 888
Kzo9I GATC 5 cut(s) 490, 542, 554, 569, 904
LguI GCTCTTC 2 cut(s) 28, 99
LmnI GCTCC 3 cut(s) 24, 194, 341
Lsp1109I GCAGC 4 cut(s) 53, 75, 763, 1171
LweI GCATC 2 cut(s) 219, 353
MaeI CTAG 2 cut(s) 851, 1040
MaeII ACGT 4 cut(s) 482, 586, 803, 983
MaeIII GTNAC 2 cut(s) 694, 977
MalI GATC 5 cut(s) 492, 544, 556, 571, 906
MboI GATC 5 cut(s) 490, 542, 554, 569, 904
MboII GAAGA 8 cut(s) 45, 86, 146, 399, 564, 722, 767, 1081
MfeI CAATTG 1 cut(s) 957
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 2 cut(s) 161, 349
MmeI TCCRAC 1 cut(s) 544
Mox20I TGGCCA 1 cut(s) 611
Mph1103I ATGCAT 2 cut(s) 521, 1065
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 611
MseI TTAA 7 cut(s) 378, 413, 687, 887, 918, 1113, 1143
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 2 cut(s) 266, 1119
MunI CAATTG 1 cut(s) 957
Mva1269I GAATGC 2 cut(s) 519, 521
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 17, 598, 1181, 1190
NdeII GATC 5 cut(s) 490, 542, 554, 569, 904
NheI GCTAGC 1 cut(s) 1039
NlaIII CATG 6 cut(s) 24, 185, 459, 667, 1034, 1201
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 1 cut(s) 39
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 2 cut(s) 521, 1065
NspI RCATGY 1 cut(s) 1201
PagI TCATGA 1 cut(s) 1030
PaqCI CACCTGC 2 cut(s) 527, 1026
PciSI GCTCTTC 2 cut(s) 28, 99
PctI GAATGC 2 cut(s) 519, 521
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 5 cut(s) 276, 296, 797, 991, 1048
PflMI CCANNNNNTGG 1 cut(s) 854
PkrI GCNGC 4 cut(s) 65, 68, 778, 1186
PleI GAGTC 2 cut(s) 161, 349
PmaCI CACGTG 1 cut(s) 984
PmlI CACGTG 1 cut(s) 984
PpsI GAGTC 2 cut(s) 161, 349
Ppu21I YACGTR 2 cut(s) 804, 984
PshBI ATTAAT 1 cut(s) 918
PspCI CACGTG 1 cut(s) 984
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 3 cut(s) 115, 268, 429
PstI CTGCAG 1 cut(s) 781
PsuI RGATCY 3 cut(s) 542, 554, 569
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 4 cut(s) 51, 328, 938, 1007
RsaNI GTAC 4 cut(s) 50, 327, 937, 1006
SapI GCTCTTC 2 cut(s) 28, 99
SaqAI TTAA 7 cut(s) 378, 413, 687, 887, 918, 1113, 1143
SatI GCNGC 4 cut(s) 64, 67, 777, 1185
Sau3AI GATC 5 cut(s) 490, 542, 554, 569, 904
Sau96I GGNCC 3 cut(s) 115, 268, 429
SchI GAGTC 2 cut(s) 161, 349
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 2 cut(s) 219, 353
SfcI CTRYAG 2 cut(s) 384, 777
SinI GGWCC 2 cut(s) 268, 429
SnaBI TACGTA 1 cut(s) 804
SsiI CCGC 3 cut(s) 432, 440, 1093
SspMI CTAG 2 cut(s) 851, 1040
StyI CCWWGG 1 cut(s) 823
TaaI ACNGT 1 cut(s) 499
TaiI ACGT 4 cut(s) 485, 589, 806, 986
TaqI TCGA 3 cut(s) 230, 351, 493
TatI WGTACW 1 cut(s) 936
TfiI GAWTC 5 cut(s) 276, 296, 797, 991, 1048
Tru1I TTAA 7 cut(s) 378, 413, 687, 887, 918, 1113, 1143
Tru9I TTAA 7 cut(s) 378, 413, 687, 887, 918, 1113, 1143
TscAI CASTG 3 cut(s) 18, 34, 742
TseI GCWGC 4 cut(s) 63, 66, 776, 1184
TspDTI ATGAA 6 cut(s) 17, 118, 459, 528, 1019, 1047
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 3 cut(s) 18, 34, 742
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 2 cut(s) 268, 429
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 2 cut(s) 5, 1145
XceI RCATGY 1 cut(s) 1201
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 851, 1040
ZraI GACGTC 1 cut(s) 483
Zsp2I ATGCAT 2 cut(s) 521, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.