RchiOBHm_Chr7g0230881

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006935
Physical Location & Seq
Reverse (-)
54505091 .. 54511939
6849 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20687

Sequence Viewer

Length: 1023 bp
ATGTTAGCTCCCTTCACTTGTAACTTAGAAGGCATCTCGACGCAGACAATATCGAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCGGGACCACGGATTCGTAGCCATTCCGAGAGATTCTATTCAATGCCTCCCAGAATGTAATAGTACCAATAAATTGGGAGCATCTTTCGAGACTCCCTGTGGTCGCAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTCTTCGTTAAGGCCTAATATATGGAGTGCTTTGGCTGATGAAGTTCCTGCATGGGTGTCTGATAAGAGGAACAAGACCTCGGTTGATCGAACAGTAAGATGGCTTGACGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCGCAAGAGGTGGCCAACCGAAATGTATCAGGCTATTGGATTGGAATTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATCTTAGAAGAGGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAAGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATTTTTGATTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATCTCTCTGCTTTTCAGTTAACTAATATAGGAAGTGATCAGACAAAGATTAATTTGAGAGCAACAATGTACAGGAAAGACACTTCACCAATTGTTGAAAATTGTAAATGTTACACGTGCCAGAATCATACAAAGGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATGCATAATACTCACCACTATCTAAGTTTCTTCCACTCGGTAAGGGAGGCAATTAAGTCCGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAAGTGTTATCATCTTGCTTCTGAAGCTGCTGTTGCCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

340

Amino Acids

37.71

Weight (kDa)

5.9

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 14 - 326 1.2e-81 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 350, 846
Acc16I TGCGCA 1 cut(s) 423
Acc36I ACCTGC 2 cut(s) 350, 846
AccB7I CCANNNNNTGG 1 cut(s) 674
AciI CCGC 1 cut(s) 88
AclWI GGATC 4 cut(s) 372, 373, 385, 400
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 2 cut(s) 465, 965
AcuI CTGAAG 1 cut(s) 1020
AcvI CACGTG 1 cut(s) 804
AfaI GTAC 3 cut(s) 151, 758, 827
AfiI CCNNNNNNNGG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 801
AgsI TTSAA 5 cut(s) 128, 221, 404, 785, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AloI GAACNNNNNNTCC 2 cut(s) 384, 416
AluBI AGCT 4 cut(s) 8, 349, 863, 1004
AluI AGCT 4 cut(s) 8, 349, 863, 1004
Alw26I GTCTC 2 cut(s) 170, 677
AlwI GGATC 4 cut(s) 372, 373, 385, 400
AoxI GGCC 2 cut(s) 239, 432
ApeKI GCWGC 2 cut(s) 596, 1004
ApoI RAATTY 2 cut(s) 465, 965
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
AseI ATTAAT 1 cut(s) 738
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 2 cut(s) 424, 498
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 3 cut(s) 765, 827, 884
AsuNHI GCTAGC 1 cut(s) 859
AvaII GGWCC 1 cut(s) 91
BalI TGGCCA 1 cut(s) 434
BamHI GGATCC 1 cut(s) 377
BbrPI CACGTG 1 cut(s) 804
BbvI GCAGC 2 cut(s) 583, 991
BccI CCATC 3 cut(s) 321, 642, 668
BclI TGATCA 1 cut(s) 724
BcoDI GTCTC 2 cut(s) 170, 677
BfaI CTAG 2 cut(s) 671, 860
BfmI CTRYAG 2 cut(s) 207, 597
BfoI RGCGCY 1 cut(s) 499
BfuAI ACCTGC 2 cut(s) 350, 846
BisI GCNGC 2 cut(s) 597, 1005
BlsI GCNGC 2 cut(s) 598, 1006
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 92, 379
BmsI GCATC 3 cut(s) 42, 66, 176
BmtI GCTAGC 1 cut(s) 863
BsaAI YACGTR 2 cut(s) 624, 804
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 3 cut(s) 94, 306, 643
BsaWI WCCGGW 1 cut(s) 938
Bsc4I CCNNNNNNNGG 1 cut(s) 674
BseDI CCNNGG 3 cut(s) 94, 306, 643
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMII CTCAG 2 cut(s) 878, 963
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 2 cut(s) 583, 991
BseYI CCCAGC 1 cut(s) 79
BshFI GGCC 2 cut(s) 241, 434
BsiSI CCGG 1 cut(s) 939
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 2 cut(s) 170, 677
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 2 cut(s) 342, 344
BsnI GGCC 2 cut(s) 241, 434
Bso31I GGTCTC 1 cut(s) 677
Bsp1407I TGTACA 1 cut(s) 756
Bsp143I GATC 5 cut(s) 313, 365, 377, 392, 724
BspACI CCGC 1 cut(s) 88
BspANI GGCC 2 cut(s) 241, 434
BspCNI CTCAG 2 cut(s) 877, 964
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 2 cut(s) 92, 379
BspMAI CTGCAG 1 cut(s) 601
BspMI ACCTGC 2 cut(s) 350, 846
BspOI GCTAGC 1 cut(s) 863
BspPI GGATC 4 cut(s) 372, 373, 385, 400
BspTNI GGTCTC 1 cut(s) 677
BsrGI TGTACA 1 cut(s) 756
BssECI CCNNGG 3 cut(s) 94, 306, 643
BssMI GATC 5 cut(s) 313, 365, 377, 392, 724
BssT1I CCWWGG 1 cut(s) 643
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 2 cut(s) 528, 570
BstAPI GCANNNNNTGC 1 cut(s) 76
BstAUI TGTACA 1 cut(s) 756
BstBAI YACGTR 2 cut(s) 624, 804
BstC8I GCNNGC 2 cut(s) 861, 1015
BstDEI CTNAG 5 cut(s) 25, 529, 864, 902, 972
BstDSI CCRYGG 1 cut(s) 94
BstF5I GGATG 1 cut(s) 496
BstH2I RGCGCY 1 cut(s) 499
BstHHI GCGC 2 cut(s) 424, 498
BstKTI GATC 5 cut(s) 316, 368, 380, 395, 727
BstMAI GTCTC 2 cut(s) 170, 677
BstMBI GATC 5 cut(s) 313, 365, 377, 392, 724
BstMWI GCNNNNNNNGC 5 cut(s) 76, 85, 421, 1001, 1010
BstNSI RCATGY 1 cut(s) 1021
BstSFI CTRYAG 2 cut(s) 207, 597
BstSNI TACGTA 1 cut(s) 624
BstV1I GCAGC 2 cut(s) 583, 991
BstX2I RGATCY 3 cut(s) 365, 377, 392
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 3 cut(s) 365, 377, 392
BsuRI GGCC 2 cut(s) 241, 434
BtgI CCRYGG 1 cut(s) 94
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 562
BveI ACCTGC 2 cut(s) 350, 846
Cac8I GCNNGC 2 cut(s) 861, 1015
CciI TCATGA 1 cut(s) 850
CfoI GCGC 2 cut(s) 424, 498
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 1 cut(s) 49
Csp6I GTAC 3 cut(s) 150, 757, 826
CviAII CATG 4 cut(s) 279, 487, 851, 1018
CviQI GTAC 3 cut(s) 150, 757, 826
DdeI CTNAG 5 cut(s) 25, 529, 864, 902, 972
DpnI GATC 5 cut(s) 315, 367, 379, 394, 726
DpnII GATC 5 cut(s) 313, 365, 377, 392, 724
EaeI YGGCCR 1 cut(s) 432
Eam1104I CTCTTC 2 cut(s) 528, 570
EarI CTCTTC 2 cut(s) 528, 570
Eco105I TACGTA 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 643
Eco147I AGGCCT 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 677
Eco32I GATATC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 1020
Eco72I CACGTG 1 cut(s) 804
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 643
EcoT22I ATGCAT 2 cut(s) 344, 885
ErhI CCWWGG 1 cut(s) 643
FaeI CATG 4 cut(s) 282, 490, 854, 1021
FaqI GGGAC 1 cut(s) 104
FatI CATG 4 cut(s) 278, 486, 850, 1017
FauI CCCGC 1 cut(s) 81
FbaI TGATCA 1 cut(s) 724
Fnu4HI GCNGC 2 cut(s) 597, 1005
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 2 cut(s) 597, 1005
FspBI CTAG 2 cut(s) 671, 860
FspI TGCGCA 1 cut(s) 423
GlaI GCGC 2 cut(s) 423, 497
GluI GCNGC 2 cut(s) 597, 1005
GsaI CCCAGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 499
HaeIII GGCC 2 cut(s) 241, 434
HapII CCGG 1 cut(s) 939
HgaI GACGC 1 cut(s) 49
HhaI GCGC 2 cut(s) 424, 498
Hin1II CATG 4 cut(s) 282, 490, 854, 1021
Hin6I GCGC 2 cut(s) 422, 496
HinP1I GCGC 2 cut(s) 422, 496
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 6 cut(s) 99, 119, 178, 617, 811, 868
HpaI GTTAAC 1 cut(s) 708
HpaII CCGG 1 cut(s) 939
HphI GGTGA 3 cut(s) 765, 827, 884
Hpy166II GTNNAC 1 cut(s) 708
Hpy188I TCNGA 7 cut(s) 114, 289, 729, 867, 959, 973, 1000
Hpy188III TCNNGA 5 cut(s) 37, 175, 404, 572, 851
Hpy8I GTNNAC 1 cut(s) 708
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 3 cut(s) 22, 23, 673
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 3 cut(s) 409, 623, 803
HpyCH4V TGCA 8 cut(s) 70, 278, 342, 586, 599, 656, 883, 1017
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 85, 421, 1001, 1010
HpyF3I CTNAG 5 cut(s) 25, 529, 864, 902, 972
HpySE526I ACGT 3 cut(s) 409, 623, 803
Hsp92II CATG 4 cut(s) 282, 490, 854, 1021
HspAI GCGC 2 cut(s) 422, 496
Ksp22I TGATCA 1 cut(s) 724
KspAI GTTAAC 1 cut(s) 708
Kzo9I GATC 5 cut(s) 313, 365, 377, 392, 724
LmnI GCTCC 2 cut(s) 13, 164
Lsp1109I GCAGC 2 cut(s) 583, 991
LweI GCATC 3 cut(s) 42, 66, 176
MaeI CTAG 2 cut(s) 671, 860
MaeII ACGT 3 cut(s) 409, 623, 803
MaeIII GTNAC 3 cut(s) 20, 517, 797
MalI GATC 5 cut(s) 315, 367, 379, 394, 726
MboI GATC 5 cut(s) 313, 365, 377, 392, 724
MboII GAAGA 5 cut(s) 222, 387, 545, 587, 901
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 3 cut(s) 365, 377, 392
MlsI TGGCCA 1 cut(s) 434
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 367
Mox20I TGGCCA 1 cut(s) 434
Mph1103I ATGCAT 2 cut(s) 344, 885
MroXI GAANNNNTTC 1 cut(s) 342
MscI TGGCCA 1 cut(s) 434
MseI TTAA 6 cut(s) 201, 236, 510, 707, 738, 933
Msp20I TGGCCA 1 cut(s) 434
MspI CCGG 1 cut(s) 939
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 2 cut(s) 342, 344
MwoI GCNNNNNNNGC 5 cut(s) 76, 85, 421, 1001, 1010
NdeII GATC 5 cut(s) 313, 365, 377, 392, 724
NheI GCTAGC 1 cut(s) 859
NlaIII CATG 4 cut(s) 282, 490, 854, 1021
NlaIV GGNNCC 2 cut(s) 92, 379
NsbI TGCGCA 1 cut(s) 423
NsiI ATGCAT 2 cut(s) 344, 885
NspI RCATGY 1 cut(s) 1021
PagI TCATGA 1 cut(s) 850
PaqCI CACCTGC 2 cut(s) 350, 846
PceI AGGCCT 1 cut(s) 241
PctI GAATGC 2 cut(s) 342, 344
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 5 cut(s) 99, 119, 617, 811, 868
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 2 cut(s) 598, 1006
PleI GAGTC 1 cut(s) 172
PmaCI CACGTG 1 cut(s) 804
PmlI CACGTG 1 cut(s) 804
PpsI GAGTC 1 cut(s) 172
Ppu21I YACGTR 2 cut(s) 624, 804
PshBI ATTAAT 1 cut(s) 738
PspCI CACGTG 1 cut(s) 804
PspFI CCCAGC 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 92, 379
PspPI GGNCC 1 cut(s) 91
PstI CTGCAG 1 cut(s) 601
PsuI RGATCY 3 cut(s) 365, 377, 392
RsaI GTAC 3 cut(s) 151, 758, 827
RsaNI GTAC 3 cut(s) 150, 757, 826
SaqAI TTAA 6 cut(s) 201, 236, 510, 707, 738, 933
SatI GCNGC 2 cut(s) 597, 1005
Sau3AI GATC 5 cut(s) 313, 365, 377, 392, 724
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 42, 66, 176
SfcI CTRYAG 2 cut(s) 207, 597
SinI GGWCC 1 cut(s) 91
SnaBI TACGTA 1 cut(s) 624
SseBI AGGCCT 1 cut(s) 241
SsiI CCGC 1 cut(s) 88
SspMI CTAG 2 cut(s) 671, 860
StuI AGGCCT 1 cut(s) 241
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 3 cut(s) 412, 626, 806
TaqI TCGA 4 cut(s) 38, 53, 174, 316
TatI WGTACW 1 cut(s) 756
TfiI GAWTC 5 cut(s) 99, 119, 617, 811, 868
Tru1I TTAA 6 cut(s) 201, 236, 510, 707, 738, 933
Tru9I TTAA 6 cut(s) 201, 236, 510, 707, 738, 933
TscAI CASTG 1 cut(s) 562
TseI GCWGC 2 cut(s) 596, 1004
TspDTI ATGAA 3 cut(s) 282, 839, 867
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 562
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 91
VspI ATTAAT 1 cut(s) 738
XapI RAATTY 2 cut(s) 465, 965
XceI RCATGY 1 cut(s) 1021
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 2 cut(s) 671, 860
Zsp2I ATGCAT 2 cut(s) 344, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.