Prupe.1G546900_v2.0.a1

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0006935
Physical Location & Seq
Reverse (-)
44704863 .. 44710271
5409 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G546900.2

Sequence Viewer

Length: 1188 bp
ATGAAGTTCGCAGTGAAGGCATGGAGCAGCGGAAAAGCGCGGGCAGGGGTGCTCCAGCTGGGCAGCTGCCCGGGCCCAATAGAGACCCCTTCTCTTCTCCTCTCTACTCGAAAGGGCCTGCCCCATTTCATCCCCCCTGACCTTCTTTACTCTCTTCCTTCCCCTGATTCCCATCTCTTCCATGTTAGCTCCCTCCACTTCCTGGAAGGCCTCTCACCACAAACAATATCAAAGATAGGAGGCTTGCATCAGATGCTTGGTTTGCCTGAACACGGGTTCGTCTGCATACCAAGGGACTCTATTCAATGCCTTCCAGAATGTAATAGCAGTAACAAATTTGGAGCATCTTTTGAGACTCCTTGCGGTCGCCGTTTGATTAAACCTGTAGAGTATGTTGAGTTGATTTCTTCCTTGAGACCCAATATATGGACTACTTTGGCTGATGAAGTTCCTACGTGGGTATCTGAGAAGAGGAACAAGACCTCAGTTGATCGAACTATTAGATGGCTTGATGAATGCATTGCGCTAAATCCGGCAGGTGGACCTGTCTTTGGATCCATTGTTGGAGGGTGTTCTGTAAAAGAACGTGAACGATGTGCGCAAGAAGCTGCCCGCAGAAATTTATCAGGTTACTGGATCGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTTCTTAATGCTGTTACTGATATCTTACAAGAGGAGAAGCCACGCTTGATCACGGGACTTGGACTTCCAGAGGAGATCTTGCAGGGGGTTGCTGCAGGCATTGATGTCTTTGATTCTACGTATCTATGCCATCTTACCCTTGGTGGCTTTGCACTTACGTTCCCACTAGATGGAGTTGAGATGAATACATCTGATAATATAGGAAGTGACCAGACAAAGATTAATTTGAGAGCAACAGTTTACAGGAAAGATATATCACCCATTGTTGAAAATTGTAACTGCTATACATGCAAGAATCATACAAAAGCTTACATCAATCACCTGCTCAATGTTCACGAAATGCTGGCTCAGACTCTACTAGAAATACATAATACTCACCATTATATAGGATTCTTCTGCTCAGTAAGGGAGGCAATTAAGTCCGGTAATTTTGAGCAATTCAGACAGAAATTTGTTGAGGGTAGATGTTACCATGTTGCTGCCGAAGCTGCTGCTGCCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

396

Amino Acids

43.5

Weight (kDa)

6.4

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1011
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 2 cut(s) 527, 1011
AccB7I CCANNNNNTGG 3 cut(s) 202, 426, 851
AccII CGCG 1 cut(s) 40
AciI CCGC 4 cut(s) 30, 40, 363, 613
AclWI GGATC 3 cut(s) 549, 562, 644
AcsI RAATTY 3 cut(s) 335, 619, 1130
AfiI CCNNNNNNNGG 6 cut(s) 202, 272, 426, 539, 551, 851
AgsI TTSAA 2 cut(s) 305, 950
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 6 cut(s) 58, 66, 189, 608, 989, 1169
AluI AGCT 6 cut(s) 58, 66, 189, 608, 989, 1169
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 3 cut(s) 77, 347, 409
AlwI GGATC 3 cut(s) 549, 562, 644
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 3 cut(s) 73, 115, 208
ApaI GGGCCC 1 cut(s) 77
ApeKI GCWGC 9 cut(s) 27, 63, 66, 608, 773, 1160, 1169, 1172, 1175
ApoI RAATTY 3 cut(s) 335, 619, 1130
AseI ATTAAT 1 cut(s) 903
AspLEI GCGC 4 cut(s) 40, 526, 601, 675
AspS9I GGNCC 4 cut(s) 73, 74, 115, 542
AsuC2I CCSGG 2 cut(s) 71, 72
AsuHPI GGTGA 4 cut(s) 207, 930, 992, 1049
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 77
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BamHI GGATCC 1 cut(s) 554
BanII GRGCYC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 9 cut(s) 39, 53, 75, 595, 760, 1147, 1156, 1159, 1162
BccI CCATC 4 cut(s) 180, 498, 819, 845
BceAI ACGGC 1 cut(s) 354
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 2 cut(s) 71, 72
BcoDI GTCTC 3 cut(s) 77, 347, 409
BfaI CTAG 2 cut(s) 848, 1040
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 527, 1011
BglII AGATCT 1 cut(s) 756
BisI GCNGC 9 cut(s) 28, 64, 67, 609, 774, 1161, 1170, 1173, 1176
BlsI GCNGC 9 cut(s) 29, 65, 68, 610, 775, 1162, 1171, 1174, 1177
Bme1390I CCNGG 3 cut(s) 71, 72, 203
Bme18I GGWCC 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 73, 74, 115, 542
BmiI GGNNCC 2 cut(s) 75, 556
BmrFI CCNGG 3 cut(s) 71, 72, 203
BmsI GCATC 3 cut(s) 243, 256, 353
BpmI CTGGAG 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 2 cut(s) 71, 72
BsaAI YACGTR 2 cut(s) 456, 801
BsaBI GATNNNNATC 1 cut(s) 171
BsaI GGTCTC 2 cut(s) 77, 409
BsaJI CCNNGG 3 cut(s) 70, 290, 820
BsaWI WCCGGW 1 cut(s) 1103
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 272, 426, 539, 551, 851
Bse1I ACTGG 1 cut(s) 638
Bse3DI GCAATG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 3 cut(s) 70, 290, 820
BseGI GGATG 2 cut(s) 129, 673
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 6 cut(s) 202, 272, 426, 539, 551, 851
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 4 cut(s) 456, 498, 1043, 1095
BseNI ACTGG 1 cut(s) 638
BseRI GAGGAG 3 cut(s) 89, 728, 767
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 9 cut(s) 39, 53, 75, 595, 760, 1147, 1156, 1159, 1162
BseYI CCCAGC 1 cut(s) 58
Bsh1236I CGCG 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 3 cut(s) 75, 117, 210
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 54
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 3 cut(s) 71, 533, 1104
BslFI GGGAC 2 cut(s) 308, 750
BslI CCNNNNNNNGG 6 cut(s) 202, 272, 426, 539, 551, 851
BsmAI GTCTC 3 cut(s) 77, 347, 409
BsmFI GGGAC 2 cut(s) 308, 750
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 3 cut(s) 75, 117, 210
Bso31I GGTCTC 2 cut(s) 77, 409
BsoBI CYCGRG 1 cut(s) 70
Bsp120I GGGCCC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 54, 77
Bsp143I GATC 5 cut(s) 490, 554, 636, 729, 756
BspACI CCGC 4 cut(s) 30, 40, 363, 613
BspANI GGCC 3 cut(s) 75, 117, 210
BspCNI CTCAG 4 cut(s) 457, 497, 1042, 1094
BspFNI CGCG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 75, 556
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 527, 1011
BspPI GGATC 3 cut(s) 549, 562, 644
BspQI GCTCTTC 1 cut(s) 687
BspTNI GGTCTC 2 cut(s) 77, 409
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 1 cut(s) 638
BssECI CCNNGG 3 cut(s) 70, 290, 820
BssMI GATC 5 cut(s) 490, 554, 636, 729, 756
BssT1I CCWWGG 2 cut(s) 290, 820
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 919
Bst6I CTCTTC 5 cut(s) 99, 159, 182, 464, 687
BstAPI GCANNNNNTGC 1 cut(s) 253
BstBAI YACGTR 2 cut(s) 456, 801
BstC8I GCNNGC 7 cut(s) 42, 119, 245, 613, 778, 1026, 1180
BstDEI CTNAG 4 cut(s) 465, 484, 1029, 1081
BstF5I GGATG 2 cut(s) 129, 673
BstFNI CGCG 1 cut(s) 40
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 4 cut(s) 40, 526, 601, 675
BstKTI GATC 5 cut(s) 493, 557, 639, 732, 759
BstMAI GTCTC 3 cut(s) 77, 347, 409
BstMBI GATC 5 cut(s) 490, 554, 636, 729, 756
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 9 cut(s) 17, 72, 253, 262, 605, 969, 1166, 1169, 1175
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 972, 1186
BstSCI CCNGG 3 cut(s) 69, 70, 201
BstSFI CTRYAG 2 cut(s) 384, 774
BstSLI GKGCMC 1 cut(s) 77
BstSNI TACGTA 1 cut(s) 801
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 9 cut(s) 39, 53, 75, 595, 760, 1147, 1156, 1159, 1162
BstX2I RGATCY 2 cut(s) 554, 756
BstYI RGATCY 2 cut(s) 554, 756
BsuRI GGCC 3 cut(s) 75, 117, 210
BtsCI GGATG 2 cut(s) 129, 673
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 2 cut(s) 527, 1011
Cac8I GCNNGC 7 cut(s) 42, 119, 245, 613, 778, 1026, 1180
CfoI GCGC 4 cut(s) 40, 526, 601, 675
Cfr13I GGNCC 4 cut(s) 73, 74, 115, 542
Cfr9I CCCGGG 1 cut(s) 70
CviAII CATG 6 cut(s) 21, 182, 664, 969, 1154, 1183
DdeI CTNAG 4 cut(s) 465, 484, 1029, 1081
DpnI GATC 5 cut(s) 492, 556, 638, 731, 758
DpnII GATC 5 cut(s) 490, 554, 636, 729, 756
Eam1104I CTCTTC 5 cut(s) 99, 159, 182, 464, 687
EarI CTCTTC 5 cut(s) 99, 159, 182, 464, 687
Eco105I TACGTA 1 cut(s) 801
Eco130I CCWWGG 2 cut(s) 290, 820
Eco147I AGGCCT 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 77
Eco31I GGTCTC 2 cut(s) 77, 409
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 1 cut(s) 542
Eco88I CYCGRG 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 2 cut(s) 290, 820
EcoT22I ATGCAT 1 cut(s) 521
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 2 cut(s) 290, 820
FaeI CATG 6 cut(s) 24, 185, 667, 972, 1157, 1186
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 2 cut(s) 308, 750
FatI CATG 6 cut(s) 20, 181, 663, 968, 1153, 1182
FauI CCCGC 2 cut(s) 33, 620
FbaI TGATCA 1 cut(s) 729
Fnu4HI GCNGC 9 cut(s) 28, 64, 67, 609, 774, 1161, 1170, 1173, 1176
FokI GGATG 2 cut(s) 116, 680
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 9 cut(s) 28, 64, 67, 609, 774, 1161, 1170, 1173, 1176
FspBI CTAG 2 cut(s) 848, 1040
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 4 cut(s) 39, 525, 600, 674
GluI GCNGC 9 cut(s) 28, 64, 67, 609, 774, 1161, 1170, 1173, 1176
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 3 cut(s) 75, 117, 210
HapII CCGG 3 cut(s) 71, 533, 1104
HhaI GCGC 4 cut(s) 40, 526, 601, 675
Hin1II CATG 6 cut(s) 24, 185, 667, 972, 1157, 1186
Hin6I GCGC 4 cut(s) 38, 524, 599, 673
HinP1I GCGC 4 cut(s) 38, 524, 599, 673
HindIII AAGCTT 1 cut(s) 987
HinfI GANTC 7 cut(s) 167, 296, 355, 794, 976, 1033, 1071
HpaII CCGG 3 cut(s) 71, 533, 1104
HphI GGTGA 4 cut(s) 207, 930, 992, 1049
Hpy166II GTNNAC 4 cut(s) 542, 590, 922, 1015
Hpy188I TCNGA 6 cut(s) 252, 466, 641, 874, 1032, 1124
Hpy188III TCNNGA 3 cut(s) 314, 749, 1016
Hpy8I GTNNAC 4 cut(s) 542, 590, 922, 1015
HpyAV CCTTC 6 cut(s) 10, 99, 152, 168, 200, 320
HpyCH4III ACNGT 1 cut(s) 919
HpyCH4IV ACGT 4 cut(s) 455, 586, 800, 839
HpyCH4V TGCA 8 cut(s) 247, 285, 519, 763, 776, 833, 972, 1182
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 72, 253, 262, 605, 969, 1166, 1169, 1175
HpyF3I CTNAG 4 cut(s) 465, 484, 1029, 1081
HpySE526I ACGT 4 cut(s) 455, 586, 800, 839
Hsp92II CATG 6 cut(s) 24, 185, 667, 972, 1157, 1186
HspAI GCGC 4 cut(s) 38, 524, 599, 673
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 5 cut(s) 490, 554, 636, 729, 756
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 4 cut(s) 24, 57, 194, 341
Lsp1109I GCAGC 9 cut(s) 39, 53, 75, 595, 760, 1147, 1156, 1159, 1162
LweI GCATC 3 cut(s) 243, 256, 353
MaeI CTAG 2 cut(s) 848, 1040
MaeII ACGT 4 cut(s) 455, 586, 800, 839
MaeIII GTNAC 6 cut(s) 329, 629, 694, 887, 956, 1148
MalI GATC 5 cut(s) 492, 556, 638, 731, 758
MboI GATC 5 cut(s) 490, 554, 636, 729, 756
MboII GAAGA 7 cut(s) 86, 146, 169, 399, 481, 674, 1066
MflI RGATCY 2 cut(s) 554, 756
MhlI GDGCHC 2 cut(s) 54, 77
MluCI AATT 8 cut(s) 335, 619, 904, 952, 1095, 1108, 1118, 1130
MlyI GAGTC 3 cut(s) 290, 349, 1027
MmeI TCCRAC 1 cut(s) 544
Mph1103I ATGCAT 1 cut(s) 521
MseI TTAA 4 cut(s) 378, 687, 903, 1098
MspA1I CMGCKG 3 cut(s) 30, 58, 66
MspI CCGG 3 cut(s) 71, 533, 1104
MspR9I CCNGG 3 cut(s) 71, 72, 203
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 9 cut(s) 17, 72, 253, 262, 605, 969, 1166, 1169, 1175
NciI CCSGG 2 cut(s) 71, 72
NdeII GATC 5 cut(s) 490, 554, 636, 729, 756
NlaIII CATG 6 cut(s) 24, 185, 667, 972, 1157, 1186
NlaIV GGNNCC 2 cut(s) 75, 556
NmuCI GTSAC 1 cut(s) 887
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 2 cut(s) 972, 1186
PaqCI CACCTGC 2 cut(s) 527, 1011
PceI AGGCCT 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 687
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 4 cut(s) 167, 794, 976, 1071
PflMI CCANNNNNTGG 3 cut(s) 202, 426, 851
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 9 cut(s) 29, 65, 68, 610, 775, 1162, 1171, 1174, 1177
PleI GAGTC 3 cut(s) 290, 349, 1027
PpsI GAGTC 3 cut(s) 290, 349, 1027
Ppu21I YACGTR 2 cut(s) 456, 801
PshBI ATTAAT 1 cut(s) 903
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 75, 556
PspOMI GGGCCC 1 cut(s) 73
PspPI GGNCC 4 cut(s) 73, 74, 115, 542
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 2 cut(s) 554, 756
PvuII CAGCTG 2 cut(s) 58, 66
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 4 cut(s) 378, 687, 903, 1098
SatI GCNGC 9 cut(s) 28, 64, 67, 609, 774, 1161, 1170, 1173, 1176
Sau3AI GATC 5 cut(s) 490, 554, 636, 729, 756
Sau96I GGNCC 4 cut(s) 73, 74, 115, 542
SchI GAGTC 3 cut(s) 290, 349, 1027
ScrFI CCNGG 3 cut(s) 71, 72, 203
SduI GDGCHC 2 cut(s) 54, 77
SfaNI GCATC 3 cut(s) 243, 256, 353
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 1 cut(s) 542
SmaI CCCGGG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
SnaBI TACGTA 1 cut(s) 801
SrfI GCCCGGGC 1 cut(s) 72
Sse9I AATT 8 cut(s) 335, 619, 904, 952, 1095, 1108, 1118, 1130
SseBI AGGCCT 1 cut(s) 210
SsiI CCGC 4 cut(s) 30, 40, 363, 613
SspMI CTAG 2 cut(s) 848, 1040
StuI AGGCCT 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 69, 70, 201
StyI CCWWGG 2 cut(s) 290, 820
TaaI ACNGT 1 cut(s) 919
TaiI ACGT 4 cut(s) 458, 589, 803, 842
TaqI TCGA 2 cut(s) 109, 493
TasI AATT 8 cut(s) 335, 619, 904, 952, 1095, 1108, 1118, 1130
TfiI GAWTC 4 cut(s) 167, 794, 976, 1071
Tru1I TTAA 4 cut(s) 378, 687, 903, 1098
Tru9I TTAA 4 cut(s) 378, 687, 903, 1098
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 887
TseI GCWGC 9 cut(s) 27, 63, 66, 608, 773, 1160, 1169, 1172, 1175
Tsp45I GTSAC 1 cut(s) 887
TspDTI ATGAA 5 cut(s) 17, 118, 459, 528, 878
TspMI CCCGGG 1 cut(s) 70
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 3 cut(s) 202, 426, 851
VpaK11BI GGWCC 1 cut(s) 542
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 3 cut(s) 335, 619, 1130
XceI RCATGY 2 cut(s) 972, 1186
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmaI CCCGGG 1 cut(s) 70
XspI CTAG 2 cut(s) 848, 1040
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.