RLG00000002175

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006935
Physical Location & Seq
Reverse (-)
26769393 .. 26776445
7053 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002175

Sequence Viewer

Length: 1515 bp
ATGAAATTCGCAGTGAAGGCATGGAGCAGTGGAAGAGCACGGGCAGGGGTACTCCAGCTGGGCAGCTGCCCGAACCCAATAGAGACACCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTGCCCCTTTTCATACCCCCTGACCTTCTCCCATCTCTTCCTTCCCCCGACTCCCATCTCCTCCATCTTAGCTCCCTTCACTTCGTAGAAGGCATCTCACCTCAGACAATATCAAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCCGGACCACGGATTTGTAGCCATTCCGAGAGATTCTATTCAATGCCTCCCAGAATGTAATAGCACCAATAAATTGGGAGCATCTTTCGAGACTCCCTTTGGTCGCAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTTTTCGTTAAAGCCTAATATATGGACCGCTTTGGCGGATGAAGTTCCTGCATGGGTGTCTGATAAGAGGAACAAGACCTCTGTTGATCGAACTGTAAGATGGCTTGACGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCTAGAACGTGAGCGATGTGCGCAAGAGGTGGCTAGTCGAAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAGTGCTGTTACTGATATTTTAGAAGAGGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAGGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATCTTTGACTCTACGTATATATACCATCTTACCGTTGGAGGCTTTGCACTTACCTTCCCACTTGATGGTGTTGAGAATGTCTCTGCTTTTCAGTTAACTAATATAGGAAGTGATCAAACAAAGATTAATTTGAGAGCAACAATTTACAGGAAAGACACTTCACCAATTGCTCAAAACTGTAAATGTTACACATGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATACATAATACTCACCACTATCTAAGTTTCTTCCGCTCAGTAAGGGAGGCAATTAAGTTTGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAAGCGTTATCATCTTGCTTCTGAAGCTGCTGTTGCCTGCATTGATGTTTATGTGTCTCTTGCCTCATTGCTTTTTGTGTCTCTACCTCTGATCTCCCTCGCCTTTTTCTCCCTACTGCCCCTCTCTCCTCTTCTCGTTCACCTCCCCAAGTCCCCAACCAGGCCATACGTCGAAGCTATGACAACAAGATTAGTCGCTGCACTCGCTATTCGTGATGTGCGACGTTGGAGTTGGCTAACAATGGTTATGACATACACTTGTGTGATACTTGTCAACACGGGTTCGGACAAGATTGTCCCTCAAAAGTACATGGCTTCCATGAGTTGGGTCTGGCCTATTTCGGGGCGTTCTGTTGATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

505

Amino Acids

55.7

Weight (kDa)

8.0

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 384 8.3e-87 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 97, 527, 1020
AasI GACNNNNNNGTC 1 cut(s) 1445
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 3 cut(s) 97, 527, 1020
AccB7I CCANNNNNTGG 2 cut(s) 851, 1476
AccBSI CCGCTC 1 cut(s) 1089
AciI CCGC 3 cut(s) 432, 440, 1087
AclWI GGATC 5 cut(s) 549, 550, 562, 577, 1502
AcsI RAATTY 2 cut(s) 5, 1139
AcuI CTGAAG 1 cut(s) 1194
AfaI GTAC 3 cut(s) 51, 1001, 1460
AfiI CCNNNNNNNGG 5 cut(s) 272, 851, 1311, 1476, 1493
AgsI TTSAA 3 cut(s) 305, 398, 1052
AjnI CCWGG 1 cut(s) 1310
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AleI CACNNNNGTG 1 cut(s) 1412
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 7 cut(s) 58, 66, 189, 526, 1037, 1178, 1328
AluI AGCT 7 cut(s) 58, 66, 189, 526, 1037, 1178, 1328
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 5 cut(s) 77, 347, 871, 1212, 1236
AlwI GGATC 5 cut(s) 549, 550, 562, 577, 1502
AoxI GGCC 3 cut(s) 115, 1313, 1484
ApeKI GCWGC 5 cut(s) 63, 66, 773, 1178, 1349
ApoI RAATTY 2 cut(s) 5, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 601, 675
AspS9I GGNCC 3 cut(s) 115, 268, 429
AsuHPI GGTGA 5 cut(s) 207, 939, 1001, 1058, 1283
AsuNHI GCTAGC 1 cut(s) 1033
AvaII GGWCC 2 cut(s) 268, 429
BamHI GGATCC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 5 cut(s) 53, 75, 760, 1165, 1336
BccI CCATC 6 cut(s) 157, 180, 189, 498, 819, 845
BciT130I CCWGG 1 cut(s) 1312
BclI TGATCA 1 cut(s) 898
BcoDI GTCTC 5 cut(s) 77, 347, 871, 1212, 1236
BfaI CTAG 3 cut(s) 581, 612, 1034
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 3 cut(s) 97, 527, 1020
BisI GCNGC 5 cut(s) 64, 67, 774, 1179, 1350
BlsI GCNGC 5 cut(s) 65, 68, 775, 1180, 1351
Bme1390I CCNGG 1 cut(s) 1312
Bme18I GGWCC 2 cut(s) 268, 429
BmgT120I GGNCC 3 cut(s) 115, 268, 429
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 1 cut(s) 1312
BmsI GCATC 3 cut(s) 219, 243, 353
BmtI GCTAGC 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 851, 883
BpmI CTGGAG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 801
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 5 cut(s) 272, 851, 1311, 1476, 1493
Bse3DI GCAATG 1 cut(s) 1217
BseBI CCWGG 1 cut(s) 1312
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 2 cut(s) 448, 673
BseLI CCNNNNNNNGG 5 cut(s) 272, 851, 1311, 1476, 1493
BseMI GCAATG 1 cut(s) 1217
BseMII CTCAG 4 cut(s) 233, 1052, 1104, 1137
BseRI GAGGAG 5 cut(s) 89, 167, 728, 767, 1269
BseXI GCAGC 5 cut(s) 53, 75, 760, 1165, 1336
BseYI CCCAGC 2 cut(s) 58, 256
BsgI GTGCAG 1 cut(s) 1335
BshFI GGCC 3 cut(s) 117, 1315, 1486
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 2 cut(s) 1288, 1433
BslI CCNNNNNNNGG 5 cut(s) 272, 851, 1311, 1476, 1493
BsmAI GTCTC 5 cut(s) 77, 347, 871, 1212, 1236
BsmFI GGGAC 2 cut(s) 1288, 1433
BsmI GAATGC 2 cut(s) 519, 521
BsnI GGCC 3 cut(s) 117, 1315, 1486
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
BspACI CCGC 3 cut(s) 432, 440, 1087
BspANI GGCC 3 cut(s) 117, 1315, 1486
BspCNI CTCAG 4 cut(s) 232, 1051, 1103, 1138
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 3 cut(s) 97, 527, 1020
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 5 cut(s) 549, 550, 562, 577, 1502
BspQI GCTCTTC 2 cut(s) 28, 99
BsrBI CCGCTC 1 cut(s) 1089
BsrDI GCAATG 1 cut(s) 1217
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
Bst2UI CCWGG 1 cut(s) 1312
Bst4CI ACNGT 3 cut(s) 499, 820, 965
Bst6I CTCTTC 5 cut(s) 28, 99, 159, 705, 1287
BstAPI GCANNNNNTGC 1 cut(s) 253
BstBAI YACGTR 1 cut(s) 801
BstC8I GCNNGC 3 cut(s) 119, 1035, 1189
BstDEI CTNAG 7 cut(s) 185, 219, 686, 1038, 1076, 1090, 1146
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 448, 673
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 601, 675
BstKTI GATC 7 cut(s) 493, 545, 557, 572, 901, 1245, 1510
BstMAI GTCTC 5 cut(s) 77, 347, 871, 1212, 1236
BstMBI GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
BstMWI GCNNNNNNNGC 7 cut(s) 17, 253, 262, 598, 1175, 1184, 1355
BstNI CCWGG 1 cut(s) 1312
BstNSI RCATGY 1 cut(s) 981
BstSCI CCNGG 1 cut(s) 1310
BstSFI CTRYAG 2 cut(s) 384, 774
BstSNI TACGTA 1 cut(s) 801
BstV1I GCAGC 5 cut(s) 53, 75, 760, 1165, 1336
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 542, 554, 569
BsuRI GGCC 3 cut(s) 117, 1315, 1486
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 448, 673
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 3 cut(s) 18, 34, 739
BveI ACCTGC 3 cut(s) 97, 527, 1020
Cac8I GCNNGC 3 cut(s) 119, 1035, 1189
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 2 cut(s) 601, 675
Cfr13I GGNCC 3 cut(s) 115, 268, 429
Csp6I GTAC 3 cut(s) 50, 1000, 1459
CviAII CATG 8 cut(s) 21, 456, 664, 978, 1025, 1462, 1471, 1512
CviQI GTAC 3 cut(s) 50, 1000, 1459
DdeI CTNAG 7 cut(s) 185, 219, 686, 1038, 1076, 1090, 1146
DpnI GATC 7 cut(s) 492, 544, 556, 571, 900, 1244, 1509
DpnII GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
DrdI GACNNNNNNGTC 1 cut(s) 1445
DseDI GACNNNNNNGTC 1 cut(s) 1445
Eam1104I CTCTTC 5 cut(s) 28, 99, 159, 705, 1287
EarI CTCTTC 5 cut(s) 28, 99, 159, 705, 1287
EciI GGCGGA 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 801
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 2 cut(s) 268, 429
Eco57I CTGAAG 1 cut(s) 1194
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 1310
EcoRV GATATC 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 521
FaeI CATG 8 cut(s) 24, 459, 667, 981, 1028, 1465, 1474, 1515
FaqI GGGAC 2 cut(s) 1288, 1433
FatI CATG 8 cut(s) 20, 455, 663, 977, 1024, 1461, 1470, 1511
FbaI TGATCA 1 cut(s) 898
Fnu4HI GCNGC 5 cut(s) 64, 67, 774, 1179, 1350
FokI GGATG 2 cut(s) 455, 680
Fsp4HI GCNGC 5 cut(s) 64, 67, 774, 1179, 1350
FspBI CTAG 3 cut(s) 581, 612, 1034
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 600, 674
GluI GCNGC 5 cut(s) 64, 67, 774, 1179, 1350
GsaI CCCAGC 2 cut(s) 62, 260
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 3 cut(s) 117, 1315, 1486
HapII CCGG 1 cut(s) 266
HhaI GCGC 2 cut(s) 601, 675
Hin1II CATG 8 cut(s) 24, 459, 667, 981, 1028, 1465, 1474, 1515
Hin6I GCGC 2 cut(s) 599, 673
HinP1I GCGC 2 cut(s) 599, 673
HincII GTYRAC 2 cut(s) 882, 1426
HindII GTYRAC 2 cut(s) 882, 1426
HinfI GANTC 6 cut(s) 167, 296, 355, 794, 985, 1042
HpaI GTTAAC 1 cut(s) 882
HpaII CCGG 1 cut(s) 266
HphI GGTGA 5 cut(s) 207, 939, 1001, 1058, 1283
Hpy166II GTNNAC 3 cut(s) 882, 1291, 1426
Hpy188I TCNGA 9 cut(s) 222, 291, 466, 1041, 1133, 1147, 1174, 1242, 1438
Hpy188III TCNNGA 5 cut(s) 352, 581, 749, 1025, 1364
Hpy8I GTNNAC 3 cut(s) 882, 1291, 1426
Hpy99I CGWCG 2 cut(s) 1325, 1377
HpyAV CCTTC 6 cut(s) 10, 152, 168, 200, 203, 850
HpyCH4III ACNGT 3 cut(s) 499, 820, 965
HpyCH4IV ACGT 4 cut(s) 586, 800, 1320, 1375
HpyCH4V TGCA 8 cut(s) 247, 455, 519, 763, 776, 833, 1191, 1352
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 253, 262, 598, 1175, 1184, 1355
HpyF3I CTNAG 7 cut(s) 185, 219, 686, 1038, 1076, 1090, 1146
HpySE526I ACGT 4 cut(s) 586, 800, 1320, 1375
Hsp92II CATG 8 cut(s) 24, 459, 667, 981, 1028, 1465, 1474, 1515
HspAI GCGC 2 cut(s) 599, 673
Ksp22I TGATCA 1 cut(s) 898
KspAI GTTAAC 1 cut(s) 882
Kzo9I GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
LguI GCTCTTC 2 cut(s) 28, 99
LmnI GCTCC 3 cut(s) 24, 194, 341
Lsp1109I GCAGC 5 cut(s) 53, 75, 760, 1165, 1336
LweI GCATC 3 cut(s) 219, 243, 353
MaeI CTAG 3 cut(s) 581, 612, 1034
MaeII ACGT 4 cut(s) 586, 800, 1320, 1375
MaeIII GTNAC 2 cut(s) 694, 971
MalI GATC 7 cut(s) 492, 544, 556, 571, 900, 1244, 1509
MbiI CCGCTC 1 cut(s) 1089
MboI GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
MboII GAAGA 7 cut(s) 45, 86, 146, 564, 722, 1075, 1274
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 1 cut(s) 40
MlyI GAGTC 3 cut(s) 161, 349, 788
MmeI TCCRAC 3 cut(s) 544, 802, 1358
Mph1103I ATGCAT 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 5 cut(s) 378, 413, 881, 912, 1107
MslI CAYNNNNRTG 1 cut(s) 1412
MspA1I CMGCKG 2 cut(s) 58, 66
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 1312
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 2 cut(s) 519, 521
MvaI CCWGG 1 cut(s) 1312
MwoI GCNNNNNNNGC 7 cut(s) 17, 253, 262, 598, 1175, 1184, 1355
NdeII GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
NheI GCTAGC 1 cut(s) 1033
NlaIII CATG 8 cut(s) 24, 459, 667, 981, 1028, 1465, 1474, 1515
NlaIV GGNNCC 1 cut(s) 556
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 1 cut(s) 981
OliI CACNNNNGTG 1 cut(s) 1412
PagI TCATGA 1 cut(s) 1024
PaqCI CACCTGC 3 cut(s) 97, 527, 1020
PciSI GCTCTTC 2 cut(s) 28, 99
PcsI WCGNNNNNNNCGW 1 cut(s) 1369
PctI GAATGC 2 cut(s) 519, 521
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 3 cut(s) 296, 985, 1042
PflMI CCANNNNNTGG 2 cut(s) 851, 1476
PkrI GCNGC 5 cut(s) 65, 68, 775, 1180, 1351
PleI GAGTC 3 cut(s) 161, 349, 788
PpsI GAGTC 3 cut(s) 161, 349, 788
Ppu21I YACGTR 1 cut(s) 801
PshBI ATTAAT 1 cut(s) 912
Psp6I CCWGG 1 cut(s) 1310
PspFI CCCAGC 2 cut(s) 58, 256
PspGI CCWGG 1 cut(s) 1310
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 3 cut(s) 115, 268, 429
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 3 cut(s) 542, 554, 569
PvuII CAGCTG 2 cut(s) 58, 66
RsaI GTAC 3 cut(s) 51, 1001, 1460
RsaNI GTAC 3 cut(s) 50, 1000, 1459
RseI CAYNNNNRTG 1 cut(s) 1412
SapI GCTCTTC 2 cut(s) 28, 99
SaqAI TTAA 5 cut(s) 378, 413, 881, 912, 1107
SatI GCNGC 5 cut(s) 64, 67, 774, 1179, 1350
Sau3AI GATC 7 cut(s) 490, 542, 554, 569, 898, 1242, 1507
Sau96I GGNCC 3 cut(s) 115, 268, 429
SchI GAGTC 3 cut(s) 161, 349, 788
ScrFI CCNGG 1 cut(s) 1312
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 3 cut(s) 219, 243, 353
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 2 cut(s) 268, 429
SmiMI CAYNNNNRTG 1 cut(s) 1412
SnaBI TACGTA 1 cut(s) 801
SsiI CCGC 3 cut(s) 432, 440, 1087
SspMI CTAG 3 cut(s) 581, 612, 1034
StyD4I CCNGG 1 cut(s) 1310
TaaI ACNGT 3 cut(s) 499, 820, 965
TaiI ACGT 4 cut(s) 589, 803, 1323, 1378
TaqI TCGA 4 cut(s) 351, 493, 616, 1323
TatI WGTACW 1 cut(s) 1458
TfiI GAWTC 3 cut(s) 296, 985, 1042
Tru1I TTAA 5 cut(s) 378, 413, 881, 912, 1107
Tru9I TTAA 5 cut(s) 378, 413, 881, 912, 1107
TscAI CASTG 3 cut(s) 18, 34, 739
TseI GCWGC 5 cut(s) 63, 66, 773, 1178, 1349
TspDTI ATGAA 5 cut(s) 17, 118, 459, 1013, 1041
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 3 cut(s) 18, 34, 739
Van91I CCANNNNNTGG 2 cut(s) 851, 1476
VpaK11BI GGWCC 2 cut(s) 268, 429
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 2 cut(s) 5, 1139
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 1 cut(s) 981
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 3 cut(s) 581, 612, 1034
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.