MD15G1399400.v1.1

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006935
Physical Location & Seq
Reverse (-)
50023180 .. 50027201
4022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1399400.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGGGAGAAAATTGGGTCAGAGACTCGCAAAACCCTTGTATTTACCGCCAAAAGAGAGCAAATTCTGCAAAACCCTACAACTCCTCCTGCCGCTCTCCGACTCTAAATCCAAAGCTGTTTGTCTCTGTCGATATGAAATTCGCGGTGAAGGCATGGAGCGGCGGAAGAGCGCGGACCGGGGTTCTCCACTTGGGCAGCTGCCCGACCCCAATAGAGACCCCTTCTCTTCTCCTATCCACCCGAAAGGGCCTGCCCCAGTTCACCCCTCCTGACCTTCTTCCTTCTCTTCCTTTCCCTGATTCCCGTCTCTTCCATGTTAGCTCCCTCCACTTCTTGGAAGTCCCCTCACCTCAAACAATTTCAAAAATAGGAGGACTGCATCAGTTGCTCGGCTTGCCCGAACACGGGTTCGTGTGCATTCCAAGGGACTCCATTCAATGCCTTCCTGAATGTAATAGCAGTAACAAATTTGGAGCATCTTTCGAGACTCCTTCTGGTCGCCATTTGATGAAACCCGTTGAGTATGTCGAGTTGATTACTTGCTTGAAGCCCAATATGTGGACCACTTTGGCAGATGAAGTTCCTGTATGGGTCTCTGAGAAGAGGAACAAGACCTCGGTTGATCGAACTATAAGATGGCTTGATGAATGCATTGAGCTAAATCAGGCAGGTGGACCTGTCTTTGGATCTATTGTTGGTGGGTCTTCTGTAAATGAACGTGAGAGATGTGCCCAAGAGGTGGCTAGCCGAAATGTATCAGGTTATTGGATCGGAGGATTTGGGCTTGGAGAGAACATTTATGAGCGTCCTGCTCTCCTTAATGCTGTTACTGATATCTTACAAGAAGAGAAACCACGCTTGATCAGTGGGCTTGGACTTCCAGAGGAGGTCTTGCAGGGCGTTGCTGCCGGCATTGATGTCTTTGATTCAACGTACATATGCCATCTTACCCTTGGCGGCTTTGCACTTACGTTTCCCCTTGATGGAGTTGAGATGAATGCATCTGGTATTGAGTTGACTAATATTGGAACTGACCAGACAAAGATTAATCTGAGAGCAACAGCTTACAGGAAAGATATGTCACCCATTGTTGCAAATTGTAACTGCTACACGTGCAAGAATCATACAAAGGCGTACATCAATCACCTGCTCAATGTTCGCGAAATGCTGGCTCAGACTCTACTAGAAATACATAATACCCACCATTATCTAGGATTCTTCCGTTCTATACGGGAAGCGATTAAGTCCGGTAATTTTGACCAGTTCAGACAGAAATTCGTTGAGAGTAGATGTTATCATGTTGCTGCTGAAGCTGCTGCTGCTGCCGCCTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

446

Amino Acids

49.26

Weight (kDa)

7.55

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 56 - 430 5.4e-88 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 659, 1155
Acc36I ACCTGC 2 cut(s) 659, 1155
AccB7I CCANNNNNTGG 3 cut(s) 334, 558, 739
AccBSI CCGCTC 2 cut(s) 93, 159
AccII CGCG 3 cut(s) 143, 172, 1161
AciI CCGC 8 cut(s) 46, 91, 143, 159, 162, 172, 957, 1326
AclWI GGATC 2 cut(s) 694, 776
AcsI RAATTY 4 cut(s) 61, 137, 467, 1274
AcuI CTGAAG 1 cut(s) 1329
AcvI CACGTG 1 cut(s) 1113
AfaI GTAC 2 cut(s) 935, 1136
AfiI CCNNNNNNNGG 7 cut(s) 334, 404, 405, 558, 683, 739, 983
AflIII ACRYGT 1 cut(s) 1110
AgsI TTSAA 4 cut(s) 363, 437, 547, 930
AluBI AGCT 6 cut(s) 115, 198, 321, 658, 1064, 1313
AluI AGCT 6 cut(s) 115, 198, 321, 658, 1064, 1313
Alw26I GTCTC 6 cut(s) 15, 127, 209, 311, 479, 598
AlwI GGATC 2 cut(s) 694, 776
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 8 cut(s) 195, 198, 905, 1304, 1313, 1316, 1319, 1322
ApoI RAATTY 4 cut(s) 61, 137, 467, 1274
AseI ATTAAT 1 cut(s) 1047
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 4 cut(s) 174, 247, 561, 674
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 5 cut(s) 157, 253, 339, 1074, 1136
AsuNHI GCTAGC 1 cut(s) 743
AvaII GGWCC 3 cut(s) 174, 561, 674
BaeGI GKGCMC 1 cut(s) 733
BbrPI CACGTG 1 cut(s) 1113
BbsI GAAGAC 1 cut(s) 696
BbvI GCAGC 8 cut(s) 185, 207, 892, 1291, 1300, 1303, 1306, 1309
BccI CCATC 3 cut(s) 630, 951, 977
BclI TGATCA 1 cut(s) 861
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 6 cut(s) 15, 127, 209, 311, 479, 598
BfaI CTAG 3 cut(s) 744, 1184, 1211
BfuAI ACCTGC 2 cut(s) 659, 1155
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 3 cut(s) 174, 561, 674
BmgT120I GGNCC 4 cut(s) 174, 247, 561, 674
BmrFI CCNGG 1 cut(s) 178
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 3 cut(s) 388, 485, 1010
BmtI GCTAGC 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 696
BpuMI CCSGG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 1113
BsaI GGTCTC 2 cut(s) 209, 598
BsaJI CCNNGG 4 cut(s) 177, 422, 615, 952
BsaWI WCCGGW 1 cut(s) 1247
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 7 cut(s) 334, 404, 405, 558, 683, 739, 983
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 2 cut(s) 256, 1261
BseDI CCNNGG 4 cut(s) 177, 422, 615, 952
BseLI CCNNNNNNNGG 7 cut(s) 334, 404, 405, 558, 683, 739, 983
BseMII CTCAG 3 cut(s) 588, 1043, 1187
BseNI ACTGG 2 cut(s) 256, 1261
BseRI GAGGAG 2 cut(s) 73, 899
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 8 cut(s) 185, 207, 892, 1291, 1300, 1303, 1306, 1309
Bsh1236I CGCG 3 cut(s) 143, 172, 1161
BshFI GGCC 1 cut(s) 249
BsiSI CCGG 3 cut(s) 177, 909, 1248
BslFI GGGAC 2 cut(s) 326, 440
BslI CCNNNNNNNGG 7 cut(s) 334, 404, 405, 558, 683, 739, 983
BsmAI GTCTC 6 cut(s) 15, 127, 209, 311, 479, 598
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 2 cut(s) 326, 440
BsmI GAATGC 3 cut(s) 417, 653, 1003
BsnI GGCC 1 cut(s) 249
Bso31I GGTCTC 2 cut(s) 209, 598
Bsp1286I GDGCHC 1 cut(s) 733
Bsp143I GATC 4 cut(s) 622, 686, 768, 861
Bsp68I TCGCGA 1 cut(s) 1161
BspACI CCGC 8 cut(s) 46, 91, 143, 159, 162, 172, 957, 1326
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 3 cut(s) 589, 1044, 1186
BspFNI CGCG 3 cut(s) 143, 172, 1161
BspMI ACCTGC 2 cut(s) 659, 1155
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 2 cut(s) 694, 776
BspQI GCTCTTC 1 cut(s) 160
BspTNI GGTCTC 2 cut(s) 209, 598
BsrBI CCGCTC 2 cut(s) 93, 159
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 2 cut(s) 256, 1261
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 4 cut(s) 177, 422, 615, 952
BssMI GATC 4 cut(s) 622, 686, 768, 861
BssT1I CCWWGG 2 cut(s) 422, 952
Bst6I CTCTTC 6 cut(s) 160, 231, 291, 314, 596, 840
BstAPI GCANNNNNTGC 2 cut(s) 65, 385
BstBAI YACGTR 1 cut(s) 1113
BstC8I GCNNGC 5 cut(s) 251, 395, 745, 910, 1170
BstDEI CTNAG 3 cut(s) 597, 1052, 1173
BstFNI CGCG 3 cut(s) 143, 172, 1161
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 4 cut(s) 625, 689, 771, 864
BstMAI GTCTC 6 cut(s) 15, 127, 209, 311, 479, 598
BstMBI GATC 4 cut(s) 622, 686, 768, 861
BstSCI CCNGG 1 cut(s) 176
BstSLI GKGCMC 1 cut(s) 733
BstUI CGCG 3 cut(s) 143, 172, 1161
BstV1I GCAGC 8 cut(s) 185, 207, 892, 1291, 1300, 1303, 1306, 1309
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuRI GGCC 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 871
BtuMI TCGCGA 1 cut(s) 1161
BveI ACCTGC 2 cut(s) 659, 1155
Cac8I GCNNGC 5 cut(s) 251, 395, 745, 910, 1170
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 4 cut(s) 174, 247, 561, 674
CpoI CGGWCCG 1 cut(s) 174
CseI GACGC 1 cut(s) 794
Csp6I GTAC 2 cut(s) 934, 1135
CspI CGGWCCG 1 cut(s) 174
CviAII CATG 4 cut(s) 153, 314, 1298, 1333
CviQI GTAC 2 cut(s) 934, 1135
DdeI CTNAG 3 cut(s) 597, 1052, 1173
DpnI GATC 4 cut(s) 624, 688, 770, 863
DpnII GATC 4 cut(s) 622, 686, 768, 861
Eam1104I CTCTTC 6 cut(s) 160, 231, 291, 314, 596, 840
EarI CTCTTC 6 cut(s) 160, 231, 291, 314, 596, 840
EciI GGCGGA 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 422, 952
Eco31I GGTCTC 2 cut(s) 209, 598
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 3 cut(s) 174, 561, 674
Eco57I CTGAAG 1 cut(s) 1329
Eco72I CACGTG 1 cut(s) 1113
EcoO109I RGGNCCY 1 cut(s) 247
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 2 cut(s) 422, 952
EcoT22I ATGCAT 2 cut(s) 653, 1003
ErhI CCWWGG 2 cut(s) 422, 952
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 4 cut(s) 156, 317, 1301, 1336
FaqI GGGAC 2 cut(s) 326, 440
FatI CATG 4 cut(s) 152, 313, 1297, 1332
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 861
FspBI CTAG 3 cut(s) 744, 1184, 1211
GlaI GCGC 1 cut(s) 171
HaeIII GGCC 1 cut(s) 249
HapII CCGG 3 cut(s) 177, 909, 1248
HgaI GACGC 1 cut(s) 794
HhaI GCGC 1 cut(s) 172
Hin1II CATG 4 cut(s) 156, 317, 1301, 1336
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HincII GTYRAC 1 cut(s) 1017
HindII GTYRAC 1 cut(s) 1017
HinfI GANTC 9 cut(s) 23, 100, 299, 428, 487, 926, 1120, 1177, 1215
HpaII CCGG 3 cut(s) 177, 909, 1248
HphI GGTGA 5 cut(s) 157, 253, 339, 1074, 1136
Hpy166II GTNNAC 4 cut(s) 261, 561, 674, 1017
Hpy188I TCNGA 7 cut(s) 20, 99, 598, 773, 1053, 1176, 1268
Hpy188III TCNNGA 5 cut(s) 269, 446, 484, 881, 1160
Hpy8I GTNNAC 4 cut(s) 261, 561, 674, 1017
HpyAV CCTTC 6 cut(s) 142, 231, 284, 291, 452, 501
HpyCH4IV ACGT 4 cut(s) 718, 932, 971, 1112
HpyCH4V TGCA 9 cut(s) 68, 379, 417, 651, 895, 965, 1001, 1094, 1116
HpyF3I CTNAG 3 cut(s) 597, 1052, 1173
HpySE526I ACGT 4 cut(s) 718, 932, 971, 1112
Hsp92II CATG 4 cut(s) 156, 317, 1301, 1336
HspAI GCGC 1 cut(s) 170
KroI GCCGGC 1 cut(s) 908
KroNI GCCGGC 1 cut(s) 910
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 4 cut(s) 622, 686, 768, 861
LguI GCTCTTC 1 cut(s) 160
LmnI GCTCC 3 cut(s) 156, 326, 473
Lsp1109I GCAGC 8 cut(s) 185, 207, 892, 1291, 1300, 1303, 1306, 1309
LweI GCATC 3 cut(s) 388, 485, 1010
MaeI CTAG 3 cut(s) 744, 1184, 1211
MaeII ACGT 4 cut(s) 718, 932, 971, 1112
MaeIII GTNAC 4 cut(s) 461, 826, 1080, 1100
MalI GATC 4 cut(s) 624, 688, 770, 863
MbiI CCGCTC 2 cut(s) 93, 159
MboI GATC 4 cut(s) 622, 686, 768, 861
MboII GAAGA 9 cut(s) 177, 218, 269, 278, 301, 613, 696, 857, 1210
MflI RGATCY 1 cut(s) 686
MhlI GDGCHC 1 cut(s) 733
MluCI AATT 8 cut(s) 10, 61, 137, 357, 467, 1096, 1252, 1274
MlyI GAGTC 5 cut(s) 17, 94, 422, 481, 1171
MmeI TCCRAC 1 cut(s) 122
Mph1103I ATGCAT 2 cut(s) 653, 1003
MroNI GCCGGC 1 cut(s) 908
MseI TTAA 3 cut(s) 819, 1047, 1242
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 3 cut(s) 177, 909, 1248
MspR9I CCNGG 1 cut(s) 178
Mva1269I GAATGC 3 cut(s) 417, 653, 1003
MvnI CGCG 3 cut(s) 143, 172, 1161
NaeI GCCGGC 1 cut(s) 910
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 938
NdeII GATC 4 cut(s) 622, 686, 768, 861
NgoMIV GCCGGC 1 cut(s) 908
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 4 cut(s) 156, 317, 1301, 1336
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 1080
NruI TCGCGA 1 cut(s) 1161
NsiI ATGCAT 2 cut(s) 653, 1003
PaqCI CACCTGC 2 cut(s) 659, 1155
PciSI GCTCTTC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 3 cut(s) 417, 653, 1003
PdiI GCCGGC 1 cut(s) 910
PfeI GAWTC 4 cut(s) 299, 926, 1120, 1215
PflMI CCANNNNNTGG 3 cut(s) 334, 558, 739
PleI GAGTC 5 cut(s) 17, 94, 422, 481, 1171
PmaCI CACGTG 1 cut(s) 1113
PmlI CACGTG 1 cut(s) 1113
PpsI GAGTC 5 cut(s) 17, 94, 422, 481, 1171
Ppu21I YACGTR 1 cut(s) 1113
PshBI ATTAAT 1 cut(s) 1047
PspCI CACGTG 1 cut(s) 1113
PspPI GGNCC 4 cut(s) 174, 247, 561, 674
PsuI RGATCY 1 cut(s) 686
PvuII CAGCTG 1 cut(s) 198
RruI TCGCGA 1 cut(s) 1161
RsaI GTAC 2 cut(s) 935, 1136
RsaNI GTAC 2 cut(s) 934, 1135
Rsr2I CGGWCCG 1 cut(s) 174
RsrII CGGWCCG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 160
SaqAI TTAA 3 cut(s) 819, 1047, 1242
Sau3AI GATC 4 cut(s) 622, 686, 768, 861
Sau96I GGNCC 4 cut(s) 174, 247, 561, 674
SchI GAGTC 5 cut(s) 17, 94, 422, 481, 1171
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 1 cut(s) 733
SfaNI GCATC 3 cut(s) 388, 485, 1010
SinI GGWCC 3 cut(s) 174, 561, 674
Sse9I AATT 8 cut(s) 10, 61, 137, 357, 467, 1096, 1252, 1274
SsiI CCGC 8 cut(s) 46, 91, 143, 159, 162, 172, 957, 1326
SspI AATATT 1 cut(s) 1024
SspMI CTAG 3 cut(s) 744, 1184, 1211
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 2 cut(s) 422, 952
TaiI ACGT 4 cut(s) 721, 935, 974, 1115
TaqI TCGA 4 cut(s) 129, 483, 528, 625
TasI AATT 8 cut(s) 10, 61, 137, 357, 467, 1096, 1252, 1274
TauI GCSGC 4 cut(s) 93, 162, 960, 1328
TfiI GAWTC 4 cut(s) 299, 926, 1120, 1215
Tru1I TTAA 3 cut(s) 819, 1047, 1242
Tru9I TTAA 3 cut(s) 819, 1047, 1242
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 1 cut(s) 1080
TseI GCWGC 8 cut(s) 195, 198, 905, 1304, 1313, 1316, 1319, 1322
Tsp45I GTSAC 1 cut(s) 1080
TspDTI ATGAA 7 cut(s) 149, 524, 591, 660, 729, 1010, 1321
TspGWI ACGGA 1 cut(s) 1211
TspRI CASTG 1 cut(s) 871
Van91I CCANNNNNTGG 3 cut(s) 334, 558, 739
VpaK11BI GGWCC 3 cut(s) 174, 561, 674
VspI ATTAAT 1 cut(s) 1047
XapI RAATTY 4 cut(s) 61, 137, 467, 1274
XcmI CCANNNNNNNNNTGG 1 cut(s) 950
XspI CTAG 3 cut(s) 744, 1184, 1211
Zsp2I ATGCAT 2 cut(s) 653, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.