Rh7BG330800

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006935
Physical Location & Seq
Forward (+)
34240783 .. 34250082
9300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG330800.1

Sequence Viewer

Length: 1491 bp
ATGAAATTCGCAGTGAAGGCATGGAGCAGTGGAAGAGCACGGGCAGGGGTACTCCAGCTGGGCAGCTGCCCGAACCCAATAGAGACCCCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTGCCCCTTTTCATACCCCCTGACCTTCTCCCTTCTCTTCCTTCCCCCGACTCCCATCTCCTCCATCTTAGCTCCCTTCACTTCCTAGAAGGCATCTCACCTCAGACAATATCAAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCCGGACCACGGATTCGTAGCCATTCCGAGAGATTCTATTCAATGCCTCCCAGAATGTAATAGCACCAATAAATTGGGAGCATCTTTCGACACTCCCTGTGGTCGCAGTTTGGTTAAGCCTGTAGAGTATGTTGAATTGATTTTTTCGTTAAAGCCTAATATATGGACCGCTTTGGCGGATGAAGTTCCTGCATGGGTGTCTGATAAGAGGAACAAGACCTCTGTTGATCGAACTGTAAGATGGCTCGACGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCTATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCGCAAGAGGTGGCTAGCCGAAGTGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATTTTAGAAGAGGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAGGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATCTTTGACTCTACGTATATATACCATCTTACCGTTGGAGGCTTTGCACTTACCTTCCCACTAGACGGTGTTGAGAATGTCTCTGCTTTTCAGTTAACTAATATAGGAAGTGATCAAACAAAGATTAATTTGAGAGCAACAATTTACAGGAAAGACACTTCACCAATTGCTCAAAACTGTAAATGTTACACATGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATACATAATACTCACCACTATCTAAGTTTCTTCCGCTCAGTAAGGGAGGCAATTAAGTCTGGAAAAGTTAATGCAGCCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATGAGGTGGCAAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAAAAGTGAAGCAGCTCAAGCTCACGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAGTACGTTCCCGTTTCTAATATCCTTAGTTCACTGTGTTTTACGAGAAACAGGAACTGTCTATCTGTTCAAACATAGATGTGGTGTTTTAAGAACGGAGCCTTATCGTGTGGATGTTGACGGCCGCATCTTTTGGAAATTAAAGGGTTACAACAATGGAGAAGATATTGTGCTTCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

496

Amino Acids

54.53

Weight (kDa)

7.24

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 379 2.4e-85 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1020
Acc16I TGCGCA 1 cut(s) 600
Acc36I ACCTGC 2 cut(s) 527, 1020
AccBSI CCGCTC 1 cut(s) 1089
AciI CCGC 5 cut(s) 432, 440, 1087, 1156, 1432
AclWI GGATC 3 cut(s) 550, 562, 577
AcoI YGGCCR 1 cut(s) 1429
AcsI RAATTY 1 cut(s) 5
AfaI GTAC 3 cut(s) 51, 1001, 1312
AfiI CCNNNNNNNGG 2 cut(s) 272, 851
AgsI TTSAA 7 cut(s) 305, 398, 581, 1052, 1286, 1378, 1484
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 9 cut(s) 58, 66, 189, 526, 1037, 1183, 1232, 1262, 1268
AluI AGCT 9 cut(s) 58, 66, 189, 526, 1037, 1183, 1232, 1262, 1268
Alw21I GWGCWC 2 cut(s) 40, 1207
Alw26I GTCTC 2 cut(s) 77, 871
AlwI GGATC 3 cut(s) 550, 562, 577
AlwNI CAGNNNCTG 1 cut(s) 1364
AoxI GGCC 2 cut(s) 115, 1429
ApeKI GCWGC 5 cut(s) 63, 66, 773, 1127, 1259
ApoI RAATTY 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 601, 675
AspS9I GGNCC 3 cut(s) 115, 268, 429
AsuHPI GGTGA 4 cut(s) 207, 939, 1001, 1058
AsuNHI GCTAGC 2 cut(s) 611, 1033
AvaII GGWCC 2 cut(s) 268, 429
Bbv12I GWGCWC 2 cut(s) 40, 1207
BbvI GCAGC 5 cut(s) 53, 75, 760, 1139, 1271
BccI CCATC 4 cut(s) 180, 189, 498, 819
BceAI ACGGC 1 cut(s) 1444
BclI TGATCA 1 cut(s) 898
BcoDI GTCTC 2 cut(s) 77, 871
BfaI CTAG 4 cut(s) 203, 612, 848, 1034
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 527, 1020
BisI GCNGC 6 cut(s) 64, 67, 774, 1128, 1260, 1432
BlsI GCNGC 6 cut(s) 65, 68, 775, 1129, 1261, 1433
Bme18I GGWCC 2 cut(s) 268, 429
BmgT120I GGNCC 3 cut(s) 115, 268, 429
BmiI GGNNCC 1 cut(s) 1407
BmsI GCATC 4 cut(s) 219, 243, 353, 1443
BmtI GCTAGC 2 cut(s) 615, 1037
BplI GAGNNNNNCTC 2 cut(s) 851, 883
BpmI CTGGAG 1 cut(s) 38
BpuEI CTTGAG 2 cut(s) 1136, 1248
BsaAI YACGTR 1 cut(s) 801
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 851
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 3 cut(s) 448, 673, 1426
BseLI CCNNNNNNNGG 2 cut(s) 272, 851
BseMII CTCAG 4 cut(s) 233, 1052, 1104, 1266
BseRI GAGGAG 6 cut(s) 89, 167, 728, 767, 1139, 1197
BseX3I CGGCCG 1 cut(s) 1429
BseXI GCAGC 5 cut(s) 53, 75, 760, 1139, 1271
BseYI CCCAGC 2 cut(s) 58, 256
Bsh1285I CGRYCG 1 cut(s) 1432
BshFI GGCC 2 cut(s) 117, 1431
BsiEI CGRYCG 1 cut(s) 1432
BsiHKAI GWGCWC 2 cut(s) 40, 1207
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 272, 851
BsmAI GTCTC 2 cut(s) 77, 871
BsmI GAATGC 2 cut(s) 519, 521
BsnI GGCC 2 cut(s) 117, 1431
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 40, 1207
Bsp143I GATC 5 cut(s) 490, 542, 554, 569, 898
BspACI CCGC 5 cut(s) 432, 440, 1087, 1156, 1432
BspANI GGCC 2 cut(s) 117, 1431
BspCNI CTCAG 4 cut(s) 232, 1051, 1103, 1267
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 1 cut(s) 1407
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 527, 1020
BspOI GCTAGC 2 cut(s) 615, 1037
BspPI GGATC 3 cut(s) 550, 562, 577
BspQI GCTCTTC 2 cut(s) 28, 99
BspTNI GGTCTC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 1089
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 5 cut(s) 490, 542, 554, 569, 898
Bst4CI ACNGT 6 cut(s) 499, 820, 854, 965, 1343, 1366
Bst6I CTCTTC 4 cut(s) 28, 99, 159, 705
BstAPI GCANNNNNTGC 1 cut(s) 253
BstBAI YACGTR 1 cut(s) 801
BstC8I GCNNGC 3 cut(s) 119, 613, 1035
BstDEI CTNAG 7 cut(s) 185, 219, 1038, 1076, 1090, 1275, 1333
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 3 cut(s) 448, 673, 1426
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 601, 675
BstKTI GATC 5 cut(s) 493, 545, 557, 572, 901
BstMAI GTCTC 2 cut(s) 77, 871
BstMBI GATC 5 cut(s) 490, 542, 554, 569, 898
BstMCI CGRYCG 1 cut(s) 1432
BstMWI GCNNNNNNNGC 6 cut(s) 17, 253, 262, 598, 1162, 1265
BstNSI RCATGY 1 cut(s) 981
BstSFI CTRYAG 2 cut(s) 384, 774
BstSNI TACGTA 1 cut(s) 801
BstV1I GCAGC 5 cut(s) 53, 75, 760, 1139, 1271
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 542, 554, 569
BstZI CGGCCG 1 cut(s) 1429
BsuRI GGCC 2 cut(s) 117, 1431
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 3 cut(s) 448, 673, 1426
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 4 cut(s) 18, 34, 739, 1339
BveI ACCTGC 2 cut(s) 527, 1020
Cac8I GCNNGC 3 cut(s) 119, 613, 1035
CaiI CAGNNNCTG 1 cut(s) 1364
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 2 cut(s) 601, 675
Cfr13I GGNCC 3 cut(s) 115, 268, 429
Csp6I GTAC 3 cut(s) 50, 1000, 1311
CviAII CATG 6 cut(s) 21, 456, 664, 978, 1025, 1226
CviQI GTAC 3 cut(s) 50, 1000, 1311
DdeI CTNAG 7 cut(s) 185, 219, 1038, 1076, 1090, 1275, 1333
DpnI GATC 5 cut(s) 492, 544, 556, 571, 900
DpnII GATC 5 cut(s) 490, 542, 554, 569, 898
EaeI YGGCCR 1 cut(s) 1429
EagI CGGCCG 1 cut(s) 1429
Eam1104I CTCTTC 4 cut(s) 28, 99, 159, 705
EarI CTCTTC 4 cut(s) 28, 99, 159, 705
EciI GGCGGA 1 cut(s) 455
EclXI CGGCCG 1 cut(s) 1429
Eco105I TACGTA 1 cut(s) 801
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 2 cut(s) 268, 429
Eco52I CGGCCG 1 cut(s) 1429
EcoO109I RGGNCCY 1 cut(s) 115
EcoRV GATATC 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 521
FaeI CATG 6 cut(s) 24, 459, 667, 981, 1028, 1229
FatI CATG 6 cut(s) 20, 455, 663, 977, 1024, 1225
FbaI TGATCA 1 cut(s) 898
Fnu4HI GCNGC 6 cut(s) 64, 67, 774, 1128, 1260, 1432
FokI GGATG 3 cut(s) 455, 680, 1433
Fsp4HI GCNGC 6 cut(s) 64, 67, 774, 1128, 1260, 1432
FspBI CTAG 4 cut(s) 203, 612, 848, 1034
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 600, 674
GluI GCNGC 6 cut(s) 64, 67, 774, 1128, 1260, 1432
GsaI CCCAGC 2 cut(s) 62, 260
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 2 cut(s) 117, 1431
HapII CCGG 1 cut(s) 266
HhaI GCGC 2 cut(s) 601, 675
Hin1II CATG 6 cut(s) 24, 459, 667, 981, 1028, 1229
Hin6I GCGC 2 cut(s) 599, 673
HinP1I GCGC 2 cut(s) 599, 673
HincII GTYRAC 2 cut(s) 882, 1426
HindII GTYRAC 2 cut(s) 882, 1426
HindIII AAGCTT 1 cut(s) 1181
HinfI GANTC 6 cut(s) 167, 276, 296, 794, 985, 1042
HpaI GTTAAC 1 cut(s) 882
HpaII CCGG 1 cut(s) 266
HphI GGTGA 4 cut(s) 207, 939, 1001, 1058
Hpy166II GTNNAC 3 cut(s) 882, 1339, 1426
Hpy188I TCNGA 5 cut(s) 222, 291, 466, 1041, 1222
Hpy188III TCNNGA 4 cut(s) 581, 749, 1025, 1113
Hpy8I GTNNAC 3 cut(s) 882, 1339, 1426
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 7 cut(s) 10, 152, 159, 168, 200, 203, 850
HpyCH4III ACNGT 6 cut(s) 499, 820, 854, 965, 1343, 1366
HpyCH4IV ACGT 3 cut(s) 586, 800, 1313
HpyCH4V TGCA 9 cut(s) 247, 455, 519, 763, 776, 833, 1127, 1165, 1193
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 253, 262, 598, 1162, 1265
HpyF3I CTNAG 7 cut(s) 185, 219, 1038, 1076, 1090, 1275, 1333
HpySE526I ACGT 3 cut(s) 586, 800, 1313
Hsp92II CATG 6 cut(s) 24, 459, 667, 981, 1028, 1229
HspAI GCGC 2 cut(s) 599, 673
Ksp22I TGATCA 1 cut(s) 898
KspAI GTTAAC 1 cut(s) 882
Kzo9I GATC 5 cut(s) 490, 542, 554, 569, 898
LguI GCTCTTC 2 cut(s) 28, 99
LmnI GCTCC 5 cut(s) 24, 194, 341, 1210, 1405
Lsp1109I GCAGC 5 cut(s) 53, 75, 760, 1139, 1271
LweI GCATC 4 cut(s) 219, 243, 353, 1443
MaeI CTAG 4 cut(s) 203, 612, 848, 1034
MaeII ACGT 3 cut(s) 586, 800, 1313
MaeIII GTNAC 3 cut(s) 694, 971, 1454
MalI GATC 5 cut(s) 492, 544, 556, 571, 900
MbiI CCGCTC 1 cut(s) 1089
MboI GATC 5 cut(s) 490, 542, 554, 569, 898
MboII GAAGA 7 cut(s) 45, 86, 146, 564, 722, 1075, 1481
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 2 cut(s) 40, 1207
MlyI GAGTC 2 cut(s) 161, 788
MmeI TCCRAC 2 cut(s) 544, 802
Mph1103I ATGCAT 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1122, 1397, 1448
MslI CAYNNNNRTG 1 cut(s) 1386
MspA1I CMGCKG 2 cut(s) 58, 66
MspI CCGG 1 cut(s) 266
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 2 cut(s) 519, 521
MwoI GCNNNNNNNGC 6 cut(s) 17, 253, 262, 598, 1162, 1265
NdeII GATC 5 cut(s) 490, 542, 554, 569, 898
NheI GCTAGC 2 cut(s) 611, 1033
NlaIII CATG 6 cut(s) 24, 459, 667, 981, 1028, 1229
NlaIV GGNNCC 1 cut(s) 1407
NsbI TGCGCA 1 cut(s) 600
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 1 cut(s) 981
PagI TCATGA 1 cut(s) 1024
PaqCI CACCTGC 2 cut(s) 527, 1020
PciSI GCTCTTC 2 cut(s) 28, 99
PctI GAATGC 2 cut(s) 519, 521
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 4 cut(s) 276, 296, 985, 1042
PkrI GCNGC 6 cut(s) 65, 68, 775, 1129, 1261, 1433
PleI GAGTC 2 cut(s) 161, 788
PpsI GAGTC 2 cut(s) 161, 788
Ppu21I YACGTR 1 cut(s) 801
PshBI ATTAAT 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 58, 256
PspN4I GGNNCC 1 cut(s) 1407
PspPI GGNCC 3 cut(s) 115, 268, 429
PstI CTGCAG 1 cut(s) 778
PstNI CAGNNNCTG 1 cut(s) 1364
PsuI RGATCY 3 cut(s) 542, 554, 569
PvuII CAGCTG 2 cut(s) 58, 66
RsaI GTAC 3 cut(s) 51, 1001, 1312
RsaNI GTAC 3 cut(s) 50, 1000, 1311
RseI CAYNNNNRTG 1 cut(s) 1386
SapI GCTCTTC 2 cut(s) 28, 99
SaqAI TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1122, 1397, 1448
SatI GCNGC 6 cut(s) 64, 67, 774, 1128, 1260, 1432
Sau3AI GATC 5 cut(s) 490, 542, 554, 569, 898
Sau96I GGNCC 3 cut(s) 115, 268, 429
SchI GAGTC 2 cut(s) 161, 788
SduI GDGCHC 2 cut(s) 40, 1207
SfaNI GCATC 4 cut(s) 219, 243, 353, 1443
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 2 cut(s) 268, 429
SmiMI CAYNNNNRTG 1 cut(s) 1386
SmlI CTYRAG 2 cut(s) 1151, 1263
SmoI CTYRAG 2 cut(s) 1151, 1263
SnaBI TACGTA 1 cut(s) 801
SsiI CCGC 5 cut(s) 432, 440, 1087, 1156, 1432
SspMI CTAG 4 cut(s) 203, 612, 848, 1034
TaaI ACNGT 6 cut(s) 499, 820, 854, 965, 1343, 1366
TaiI ACGT 3 cut(s) 589, 803, 1316
TaqI TCGA 3 cut(s) 351, 493, 510
TauI GCSGC 1 cut(s) 1434
TfiI GAWTC 4 cut(s) 276, 296, 985, 1042
Tru1I TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1122, 1397, 1448
Tru9I TTAA 9 cut(s) 378, 413, 687, 881, 912, 1107, 1122, 1397, 1448
TscAI CASTG 4 cut(s) 18, 34, 739, 1346
TseI GCWGC 5 cut(s) 63, 66, 773, 1127, 1259
TspDTI ATGAA 6 cut(s) 17, 118, 459, 1013, 1041, 1242
TspGWI ACGGA 2 cut(s) 288, 1418
TspRI CASTG 4 cut(s) 18, 34, 739, 1346
VpaK11BI GGWCC 2 cut(s) 268, 429
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 1 cut(s) 5
XceI RCATGY 1 cut(s) 981
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 4 cut(s) 203, 612, 848, 1034
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.