Rh7AG416100

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006935
Physical Location & Seq
Reverse (-)
55867955 .. 55874927
6973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG416100.1

Sequence Viewer

Length: 966 bp
ATGGAGACGAGTTGCAGAGTGAGGAAGAAGGAAACAAGCAGCTCCCTTCACTTCTTAGAAGGCATTTCGACGCAGACAATATCGAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCGGGACCACGGATTCGTAGCCATTCCGAGAGATTCTATTCAATGCCTCCCAGAATGTAATAGTACCAATAAATTGGGAGCATCTTTCGAGACTCCCTGTGGTCGCAGTTTGGTTAAGCCTGTAGAATGGCTTGACGAATGCATTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCACAAGAGGTGGCCAGCCGAAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTCCTTAATGCTGTTACTGATATCTTAGAAGAGGAGAAACCACGCTTGGTCAGTCGGCTTGGACTTCCAGAAGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATTTTTGATTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATCTCTCTGCTTTTCAGTTGACTAATATAGGAAGTGATCAGACAAAGATTAATTTGAGAGCAACAATGCACAGGAAAGACACTTCACCAATTGTTGAAAATTGTAAATGTTACACGTGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATGCATAATACTCACCACTATCTAAGTTTCTTCCGCTCAGTATTGGAGGCAATTAAGTCTGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAGGCGTTATCCTCTTGCTTCTGAAGCTGCTGTTACCTGCATGTATTATAGTACGAACGATTCACAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

321

Amino Acids

35.69

Weight (kDa)

6.13

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 80 - 299 4.9e-62 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 266, 762
Acc36I ACCTGC 3 cut(s) 266, 762, 938
AccB7I CCANNNNNTGG 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 831
AciI CCGC 2 cut(s) 118, 829
AclWI GGATC 4 cut(s) 288, 289, 301, 316
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 1 cut(s) 881
AcuI CTGAAG 1 cut(s) 936
AcvI CACGTG 1 cut(s) 720
AfaI GTAC 3 cut(s) 181, 743, 946
AfiI CCNNNNNNNGG 1 cut(s) 590
AflIII ACRYGT 1 cut(s) 717
AgsI TTSAA 4 cut(s) 158, 320, 701, 794
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 4 cut(s) 42, 265, 779, 920
AluI AGCT 4 cut(s) 42, 265, 779, 920
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 200, 593
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 4 cut(s) 288, 289, 301, 316
AoxI GGCC 1 cut(s) 348
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 3 cut(s) 39, 512, 920
ApoI RAATTY 1 cut(s) 881
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AseI ATTAAT 1 cut(s) 654
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 3 cut(s) 681, 743, 800
AsuNHI GCTAGC 1 cut(s) 775
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 350
BamHI GGATCC 1 cut(s) 293
BbrPI CACGTG 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 3 cut(s) 51, 499, 907
BccI CCATC 2 cut(s) 558, 584
BclI TGATCA 1 cut(s) 640
BcoDI GTCTC 2 cut(s) 200, 593
BfaI CTAG 2 cut(s) 587, 776
BfmI CTRYAG 2 cut(s) 237, 513
BfoI RGCGCY 1 cut(s) 415
BfuAI ACCTGC 3 cut(s) 266, 762, 938
BisI GCNGC 3 cut(s) 40, 513, 921
BlsI GCNGC 3 cut(s) 41, 514, 922
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 122, 295
BmsI GCATC 2 cut(s) 96, 206
BmtI GCTAGC 1 cut(s) 779
BsaAI YACGTR 2 cut(s) 540, 720
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 2 cut(s) 124, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 590
BseDI CCNNGG 2 cut(s) 124, 559
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMII CTCAG 3 cut(s) 794, 846, 879
BseRI GAGGAG 1 cut(s) 467
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 3 cut(s) 51, 499, 907
BseYI CCCAGC 1 cut(s) 109
BshFI GGCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 340
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 2 cut(s) 200, 593
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 2 cut(s) 258, 260
BsnI GGCC 1 cut(s) 350
Bso31I GGTCTC 1 cut(s) 593
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 4 cut(s) 281, 293, 308, 640
BspACI CCGC 2 cut(s) 118, 829
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 3 cut(s) 793, 845, 880
BspHI TCATGA 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 122, 295
BspMAI CTGCAG 1 cut(s) 517
BspMI ACCTGC 3 cut(s) 266, 762, 938
BspOI GCTAGC 1 cut(s) 779
BspPI GGATC 4 cut(s) 288, 289, 301, 316
BspTNI GGTCTC 1 cut(s) 593
BsrBI CCGCTC 1 cut(s) 831
BssECI CCNNGG 2 cut(s) 124, 559
BssMI GATC 4 cut(s) 281, 293, 308, 640
BssT1I CCWWGG 1 cut(s) 559
Bst6I CTCTTC 2 cut(s) 444, 486
BstAPI GCANNNNNTGC 1 cut(s) 106
BstBAI YACGTR 2 cut(s) 540, 720
BstC8I GCNNGC 2 cut(s) 352, 777
BstDEI CTNAG 6 cut(s) 55, 445, 780, 818, 832, 888
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 4 cut(s) 284, 296, 311, 643
BstMAI GTCTC 2 cut(s) 200, 593
BstMBI GATC 4 cut(s) 281, 293, 308, 640
BstMWI GCNNNNNNNGC 3 cut(s) 106, 115, 917
BstNSI RCATGY 1 cut(s) 937
BstSFI CTRYAG 2 cut(s) 237, 513
BstSLI GKGCMC 1 cut(s) 340
BstSNI TACGTA 1 cut(s) 540
BstV1I GCAGC 3 cut(s) 51, 499, 907
BstX2I RGATCY 3 cut(s) 281, 293, 308
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 3 cut(s) 281, 293, 308
BsuRI GGCC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 346
BtsCI GGATG 1 cut(s) 412
BveI ACCTGC 3 cut(s) 266, 762, 938
Cac8I GCNNGC 2 cut(s) 352, 777
CciI TCATGA 1 cut(s) 766
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 1 cut(s) 121
CseI GACGC 1 cut(s) 79
Csp6I GTAC 3 cut(s) 180, 742, 945
CviAII CATG 3 cut(s) 403, 767, 934
CviQI GTAC 3 cut(s) 180, 742, 945
DdeI CTNAG 6 cut(s) 55, 445, 780, 818, 832, 888
DpnI GATC 4 cut(s) 283, 295, 310, 642
DpnII GATC 4 cut(s) 281, 293, 308, 640
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 2 cut(s) 444, 486
EarI CTCTTC 2 cut(s) 444, 486
Eco105I TACGTA 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 559
Eco31I GGTCTC 1 cut(s) 593
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 936
Eco72I CACGTG 1 cut(s) 720
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 559
EcoT22I ATGCAT 2 cut(s) 260, 801
ErhI CCWWGG 1 cut(s) 559
FaeI CATG 3 cut(s) 406, 770, 937
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 402, 766, 933
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 640
Fnu4HI GCNGC 3 cut(s) 40, 513, 921
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 3 cut(s) 40, 513, 921
FspBI CTAG 2 cut(s) 587, 776
GlaI GCGC 1 cut(s) 413
GluI GCNGC 3 cut(s) 40, 513, 921
GsaI CCCAGC 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 1 cut(s) 350
HgaI GACGC 1 cut(s) 79
HhaI GCGC 1 cut(s) 414
Hin1II CATG 3 cut(s) 406, 770, 937
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HincII GTYRAC 1 cut(s) 624
HindII GTYRAC 1 cut(s) 624
HinfI GANTC 7 cut(s) 129, 149, 208, 533, 727, 784, 953
HphI GGTGA 3 cut(s) 681, 743, 800
Hpy166II GTNNAC 2 cut(s) 338, 624
Hpy188I TCNGA 6 cut(s) 144, 645, 783, 875, 889, 916
Hpy188III TCNNGA 4 cut(s) 205, 320, 488, 767
Hpy8I GTNNAC 2 cut(s) 338, 624
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 22, 53, 56, 589
HpyCH4IV ACGT 3 cut(s) 325, 539, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 115, 917
HpyF3I CTNAG 6 cut(s) 55, 445, 780, 818, 832, 888
HpySE526I ACGT 3 cut(s) 325, 539, 719
Hsp92II CATG 3 cut(s) 406, 770, 937
HspAI GCGC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 4 cut(s) 281, 293, 308, 640
LmnI GCTCC 2 cut(s) 47, 194
Lsp1109I GCAGC 3 cut(s) 51, 499, 907
LweI GCATC 2 cut(s) 96, 206
MaeI CTAG 2 cut(s) 587, 776
MaeII ACGT 3 cut(s) 325, 539, 719
MaeIII GTNAC 3 cut(s) 433, 713, 925
MalI GATC 4 cut(s) 283, 295, 310, 642
MbiI CCGCTC 1 cut(s) 831
MboI GATC 4 cut(s) 281, 293, 308, 640
MboII GAAGA 5 cut(s) 37, 303, 461, 503, 817
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 3 cut(s) 281, 293, 308
MhlI GDGCHC 1 cut(s) 340
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 9 cut(s) 188, 519, 655, 693, 703, 846, 859, 869, 881
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 283
Mox20I TGGCCA 1 cut(s) 350
Mph1103I ATGCAT 2 cut(s) 260, 801
MroXI GAANNNNTTC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 350
MseI TTAA 4 cut(s) 231, 426, 654, 849
MslI CAYNNNNRTG 1 cut(s) 961
Msp20I TGGCCA 1 cut(s) 350
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 2 cut(s) 258, 260
MwoI GCNNNNNNNGC 3 cut(s) 106, 115, 917
NdeII GATC 4 cut(s) 281, 293, 308, 640
NheI GCTAGC 1 cut(s) 775
NlaIII CATG 3 cut(s) 406, 770, 937
NlaIV GGNNCC 2 cut(s) 122, 295
NsiI ATGCAT 2 cut(s) 260, 801
NspI RCATGY 1 cut(s) 937
PagI TCATGA 1 cut(s) 766
PaqCI CACCTGC 2 cut(s) 266, 762
PctI GAATGC 2 cut(s) 258, 260
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 6 cut(s) 129, 149, 533, 727, 784, 953
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 3 cut(s) 41, 514, 922
PleI GAGTC 1 cut(s) 202
PmaCI CACGTG 1 cut(s) 720
PmlI CACGTG 1 cut(s) 720
PpsI GAGTC 1 cut(s) 202
Ppu21I YACGTR 2 cut(s) 540, 720
PshBI ATTAAT 1 cut(s) 654
PspCI CACGTG 1 cut(s) 720
PspFI CCCAGC 1 cut(s) 109
PspN4I GGNNCC 2 cut(s) 122, 295
PspPI GGNCC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 517
PsuI RGATCY 3 cut(s) 281, 293, 308
RsaI GTAC 3 cut(s) 181, 743, 946
RsaNI GTAC 3 cut(s) 180, 742, 945
RseI CAYNNNNRTG 1 cut(s) 961
SaqAI TTAA 4 cut(s) 231, 426, 654, 849
SatI GCNGC 3 cut(s) 40, 513, 921
Sau3AI GATC 4 cut(s) 281, 293, 308, 640
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 202
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 2 cut(s) 96, 206
SfcI CTRYAG 2 cut(s) 237, 513
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 961
SnaBI TACGTA 1 cut(s) 540
Sse9I AATT 9 cut(s) 188, 519, 655, 693, 703, 846, 859, 869, 881
SsiI CCGC 2 cut(s) 118, 829
SspMI CTAG 2 cut(s) 587, 776
StyI CCWWGG 1 cut(s) 559
TaiI ACGT 3 cut(s) 328, 542, 722
TaqI TCGA 3 cut(s) 68, 83, 204
TasI AATT 9 cut(s) 188, 519, 655, 693, 703, 846, 859, 869, 881
TfiI GAWTC 6 cut(s) 129, 149, 533, 727, 784, 953
Tru1I TTAA 4 cut(s) 231, 426, 654, 849
Tru9I TTAA 4 cut(s) 231, 426, 654, 849
TseI GCWGC 3 cut(s) 39, 512, 920
TspDTI ATGAA 2 cut(s) 755, 783
TspGWI ACGGA 1 cut(s) 141
Van91I CCANNNNNTGG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 1 cut(s) 121
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 1 cut(s) 881
XceI RCATGY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 2 cut(s) 587, 776
Zsp2I ATGCAT 2 cut(s) 260, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.