Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g32910 FvH4_5g32910 |
| malus_domestica | MD15G1399400.v1.1 |
| prunus_persica | Prupe.1G546900_v2.0.a1 Prupe.1G546900_v2.0.a1 Prupe.1G546900_v2.0.a1 Prupe.1G546900_v2.0.a1 |
| pyrus_communis | pycom15g35840 |
| rosa_chinensis | RchiOBHm_Chr7g0224381 RchiOBHm_Chr7g0230881 |
| rosa_laevigata | RLG00000001455 RLG00000002175 |
| rosa_multiflora | Rmu_sc0000483.1_g000007 Rmu_sc0000551.1_g000016 Rmu_sc0006366.1_g000006 |
| rosa_roxburghii | Rroxscaffold_3G00238700 |
| rosa_rugosa | Rorug07G0201000 Rorug07G0262800 |
| rosa_samantha | Rh7AG380700 Rh7AG416100 Rh7BG330800 Rh7BG392100 Rh7CG400100 Rh7CG435100 Rh7DG410600 |
| rosa_wichuraiana | Rw7G031120 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 527, 1020 |
| Acc16I | TGCGCA | 1 cut(s) | 600 |
| Acc36I | ACCTGC | 2 cut(s) | 527, 1020 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 851 |
| AccBSI | CCGCTC | 1 cut(s) | 1089 |
| AccII | CGCG | 3 cut(s) | 1739, 1748, 1849 |
| AciI | CCGC | 8 cut(s) | 432, 440, 1087, 1629, 1847, 1863, 1880, 1968 |
| AclWI | GGATC | 4 cut(s) | 549, 550, 562, 577 |
| AcsI | RAATTY | 2 cut(s) | 5, 1139 |
| AcuI | CTGAAG | 2 cut(s) | 1194, 1364 |
| AcyI | GRCGYC | 1 cut(s) | 1664 |
| AfaI | GTAC | 7 cut(s) | 51, 1001, 1488, 1624, 1736, 1928, 2016 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 272, 851, 1345 |
| AflIII | ACRYGT | 4 cut(s) | 1252, 1477, 1918, 2006 |
| AgsI | TTSAA | 9 cut(s) | 305, 398, 1052, 1201, 1246, 1280, 1726, 1915, 2003 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 710, 742 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 561, 593 |
| AluBI | AGCT | 7 cut(s) | 58, 66, 189, 526, 1037, 1178, 1351 |
| AluI | AGCT | 7 cut(s) | 58, 66, 189, 526, 1037, 1178, 1351 |
| Alw21I | GWGCWC | 1 cut(s) | 40 |
| Alw26I | GTCTC | 3 cut(s) | 77, 871, 1787 |
| AlwI | GGATC | 4 cut(s) | 549, 550, 562, 577 |
| AoxI | GGCC | 1 cut(s) | 115 |
| ApeKI | GCWGC | 4 cut(s) | 63, 66, 773, 1351 |
| ApoI | RAATTY | 2 cut(s) | 5, 1139 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 260, 292 |
| AseI | ATTAAT | 1 cut(s) | 912 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 519 |
| AspLEI | GCGC | 4 cut(s) | 601, 675, 1741, 1750 |
| AspS9I | GGNCC | 4 cut(s) | 115, 268, 429, 1427 |
| AsuHPI | GGTGA | 5 cut(s) | 207, 939, 1001, 1058, 1328 |
| AsuNHI | GCTAGC | 1 cut(s) | 1033 |
| AvaII | GGWCC | 3 cut(s) | 268, 429, 1427 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1284, 1317 |
| BamHI | GGATCC | 1 cut(s) | 554 |
| Bbv12I | GWGCWC | 1 cut(s) | 40 |
| BbvCI | CCTCAGC | 1 cut(s) | 1497 |
| BbvI | GCAGC | 4 cut(s) | 53, 75, 760, 1338 |
| BceAI | ACGGC | 1 cut(s) | 1856 |
| BclI | TGATCA | 3 cut(s) | 898, 1395, 1609 |
| BcoDI | GTCTC | 3 cut(s) | 77, 871, 1787 |
| BfaI | CTAG | 7 cut(s) | 581, 612, 848, 1034, 1605, 1619, 1682 |
| BfmI | CTRYAG | 2 cut(s) | 384, 774 |
| BfoI | RGCGCY | 1 cut(s) | 676 |
| BfuAI | ACCTGC | 2 cut(s) | 527, 1020 |
| BisI | GCNGC | 4 cut(s) | 64, 67, 774, 1352 |
| BlsI | GCNGC | 4 cut(s) | 65, 68, 775, 1353 |
| Bme18I | GGWCC | 3 cut(s) | 268, 429, 1427 |
| BmgT120I | GGNCC | 4 cut(s) | 115, 268, 429, 1427 |
| BmiI | GGNNCC | 4 cut(s) | 556, 1428, 1887, 1975 |
| BmrI | ACTGGG | 2 cut(s) | 1549, 1706 |
| BmsI | GCATC | 4 cut(s) | 219, 243, 353, 1483 |
| BmtI | GCTAGC | 1 cut(s) | 1037 |
| BmuI | ACTGGG | 2 cut(s) | 1549, 1706 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 851, 883 |
| BpmI | CTGGAG | 1 cut(s) | 38 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1497 |
| BsaAI | YACGTR | 1 cut(s) | 801 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1614 |
| BsaHI | GRCGYC | 1 cut(s) | 1664 |
| BsaI | GGTCTC | 1 cut(s) | 77 |
| BsaJI | CCNNGG | 2 cut(s) | 271, 1430 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 272, 851, 1345 |
| Bse1I | ACTGG | 4 cut(s) | 1544, 1630, 1654, 1701 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1614 |
| BseDI | CCNNGG | 2 cut(s) | 271, 1430 |
| BseGI | GGATG | 5 cut(s) | 448, 673, 1206, 1498, 1533 |
| BseJI | GATNNNNATC | 1 cut(s) | 1614 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 272, 851, 1345 |
| BseMII | CTCAG | 5 cut(s) | 1052, 1104, 1137, 1400, 1488 |
| BseNI | ACTGG | 4 cut(s) | 1544, 1630, 1654, 1701 |
| BseRI | GAGGAG | 5 cut(s) | 89, 167, 767, 1258, 1509 |
| BseXI | GCAGC | 4 cut(s) | 53, 75, 760, 1338 |
| BseYI | CCCAGC | 3 cut(s) | 58, 256, 652 |
| Bsh1236I | CGCG | 3 cut(s) | 1739, 1748, 1849 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1847 |
| BshFI | GGCC | 1 cut(s) | 117 |
| BsiEI | CGRYCG | 1 cut(s) | 1847 |
| BsiHKAI | GWGCWC | 1 cut(s) | 40 |
| BsiSI | CCGG | 1 cut(s) | 266 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 272, 851, 1345 |
| BsmAI | GTCTC | 3 cut(s) | 77, 871, 1787 |
| BsmBI | CGTCTC | 1 cut(s) | 1787 |
| BsmI | GAATGC | 3 cut(s) | 519, 521, 1362 |
| BsnI | GGCC | 1 cut(s) | 117 |
| Bso31I | GGTCTC | 1 cut(s) | 77 |
| Bsp1286I | GDGCHC | 1 cut(s) | 40 |
| Bsp1407I | TGTACA | 1 cut(s) | 1486 |
| BspACI | CCGC | 8 cut(s) | 432, 440, 1087, 1629, 1847, 1863, 1880, 1968 |
| BspANI | GGCC | 1 cut(s) | 117 |
| BspCNI | CTCAG | 5 cut(s) | 1051, 1103, 1138, 1401, 1489 |
| BspFNI | CGCG | 3 cut(s) | 1739, 1748, 1849 |
| BspHI | TCATGA | 1 cut(s) | 1024 |
| BspLI | GGNNCC | 4 cut(s) | 556, 1428, 1887, 1975 |
| BspMAI | CTGCAG | 1 cut(s) | 778 |
| BspMI | ACCTGC | 2 cut(s) | 527, 1020 |
| BspOI | GCTAGC | 1 cut(s) | 1037 |
| BspPI | GGATC | 4 cut(s) | 549, 550, 562, 577 |
| BspQI | GCTCTTC | 2 cut(s) | 28, 99 |
| BspTNI | GGTCTC | 1 cut(s) | 77 |
| BsrBI | CCGCTC | 1 cut(s) | 1089 |
| BsrGI | TGTACA | 1 cut(s) | 1486 |
| BsrI | ACTGG | 4 cut(s) | 1544, 1630, 1654, 1701 |
| BssECI | CCNNGG | 2 cut(s) | 271, 1430 |
| BssNI | GRCGYC | 1 cut(s) | 1664 |
| BssT1I | CCWWGG | 1 cut(s) | 1430 |
| Bst4CI | ACNGT | 4 cut(s) | 499, 820, 965, 1816 |
| Bst6I | CTCTTC | 7 cut(s) | 28, 99, 159, 1592, 1790, 1916, 2004 |
| BstACI | GRCGYC | 1 cut(s) | 1664 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 253 |
| BstAUI | TGTACA | 1 cut(s) | 1486 |
| BstBAI | YACGTR | 1 cut(s) | 801 |
| BstC8I | GCNNGC | 5 cut(s) | 119, 1035, 1189, 1383, 1752 |
| BstDEI | CTNAG | 9 cut(s) | 185, 202, 1038, 1076, 1090, 1146, 1409, 1497, 1523 |
| BstDSI | CCRYGG | 1 cut(s) | 271 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1343 |
| BstF5I | GGATG | 5 cut(s) | 448, 673, 1206, 1498, 1533 |
| BstFNI | CGCG | 3 cut(s) | 1739, 1748, 1849 |
| BstH2I | RGCGCY | 1 cut(s) | 676 |
| BstHHI | GCGC | 4 cut(s) | 601, 675, 1741, 1750 |
| BstMAI | GTCTC | 3 cut(s) | 77, 871, 1787 |
| BstMCI | CGRYCG | 1 cut(s) | 1847 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 17, 253, 262, 598, 1175, 1184, 1738, 1745, 1747 |
| BstNSI | RCATGY | 4 cut(s) | 981, 1256, 1374, 1385 |
| BstSFI | CTRYAG | 2 cut(s) | 384, 774 |
| BstSNI | TACGTA | 1 cut(s) | 801 |
| BstUI | CGCG | 3 cut(s) | 1739, 1748, 1849 |
| BstV1I | GCAGC | 4 cut(s) | 53, 75, 760, 1338 |
| BstX2I | RGATCY | 3 cut(s) | 542, 554, 569 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 337 |
| BstYI | RGATCY | 3 cut(s) | 542, 554, 569 |
| BsuRI | GGCC | 1 cut(s) | 117 |
| BtgI | CCRYGG | 1 cut(s) | 271 |
| BtgZI | GCGATG | 1 cut(s) | 607 |
| BtsCI | GGATG | 5 cut(s) | 448, 673, 1206, 1498, 1533 |
| BtsI | GCAGTG | 2 cut(s) | 18, 34 |
| BtsIMutI | CAGTG | 3 cut(s) | 18, 34, 739 |
| BveI | ACCTGC | 2 cut(s) | 527, 1020 |
| Cac8I | GCNNGC | 5 cut(s) | 119, 1035, 1189, 1383, 1752 |
| CciI | TCATGA | 1 cut(s) | 1024 |
| CfoI | GCGC | 4 cut(s) | 601, 675, 1741, 1750 |
| Cfr13I | GGNCC | 4 cut(s) | 115, 268, 429, 1427 |
| CseI | GACGC | 2 cut(s) | 1653, 1863 |
| Csp6I | GTAC | 7 cut(s) | 50, 1000, 1487, 1623, 1735, 1927, 2015 |
| CviQI | GTAC | 7 cut(s) | 50, 1000, 1487, 1623, 1735, 1927, 2015 |
| DdeI | CTNAG | 9 cut(s) | 185, 202, 1038, 1076, 1090, 1146, 1409, 1497, 1523 |
| Eam1104I | CTCTTC | 7 cut(s) | 28, 99, 159, 1592, 1790, 1916, 2004 |
| EarI | CTCTTC | 7 cut(s) | 28, 99, 159, 1592, 1790, 1916, 2004 |
| EciI | GGCGGA | 1 cut(s) | 455 |
| Eco105I | TACGTA | 1 cut(s) | 801 |
| Eco130I | CCWWGG | 1 cut(s) | 1430 |
| Eco31I | GGTCTC | 1 cut(s) | 77 |
| Eco32I | GATATC | 2 cut(s) | 787, 1309 |
| Eco47I | GGWCC | 3 cut(s) | 268, 429, 1427 |
| Eco57I | CTGAAG | 2 cut(s) | 1194, 1364 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1343 |
| EcoO109I | RGGNCCY | 2 cut(s) | 115, 1427 |
| EcoRV | GATATC | 2 cut(s) | 787, 1309 |
| EcoT14I | CCWWGG | 1 cut(s) | 1430 |
| EcoT22I | ATGCAT | 2 cut(s) | 521, 1376 |
| ErhI | CCWWGG | 1 cut(s) | 1430 |
| Esp3I | CGTCTC | 1 cut(s) | 1787 |
| FbaI | TGATCA | 3 cut(s) | 898, 1395, 1609 |
| Fnu4HI | GCNGC | 4 cut(s) | 64, 67, 774, 1352 |
| FokI | GGATG | 5 cut(s) | 455, 680, 1193, 1505, 1540 |
| Fsp4HI | GCNGC | 4 cut(s) | 64, 67, 774, 1352 |
| FspBI | CTAG | 7 cut(s) | 581, 612, 848, 1034, 1605, 1619, 1682 |
| FspI | TGCGCA | 1 cut(s) | 600 |
| GlaI | GCGC | 4 cut(s) | 600, 674, 1740, 1749 |
| GluI | GCNGC | 4 cut(s) | 64, 67, 774, 1352 |
| GsaI | CCCAGC | 3 cut(s) | 62, 260, 656 |
| GsuI | CTGGAG | 1 cut(s) | 38 |
| HaeII | RGCGCY | 1 cut(s) | 676 |
| HaeIII | GGCC | 1 cut(s) | 117 |
| HapII | CCGG | 1 cut(s) | 266 |
| HgaI | GACGC | 2 cut(s) | 1653, 1863 |
| HhaI | GCGC | 4 cut(s) | 601, 675, 1741, 1750 |
| Hin1I | GRCGYC | 1 cut(s) | 1664 |
| Hin6I | GCGC | 4 cut(s) | 599, 673, 1739, 1748 |
| HinP1I | GCGC | 4 cut(s) | 599, 673, 1739, 1748 |
| HincII | GTYRAC | 1 cut(s) | 882 |
| HindII | GTYRAC | 1 cut(s) | 882 |
| HpaI | GTTAAC | 1 cut(s) | 882 |
| HpaII | CCGG | 1 cut(s) | 266 |
| HphI | GGTGA | 5 cut(s) | 207, 939, 1001, 1058, 1328 |
| Hpy166II | GTNNAC | 2 cut(s) | 882, 1585 |
| Hpy188III | TCNNGA | 4 cut(s) | 581, 749, 1025, 1312 |
| Hpy8I | GTNNAC | 2 cut(s) | 882, 1585 |
| Hpy99I | CGWCG | 5 cut(s) | 515, 1854, 1857, 1934, 2022 |
| HpyCH4III | ACNGT | 4 cut(s) | 499, 820, 965, 1816 |
| HpyCH4IV | ACGT | 5 cut(s) | 586, 800, 1477, 1918, 2006 |
| HpyCH4V | TGCA | 9 cut(s) | 247, 455, 519, 763, 776, 833, 1191, 1374, 1508 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 17, 253, 262, 598, 1175, 1184, 1738, 1745, 1747 |
| HpyF3I | CTNAG | 9 cut(s) | 185, 202, 1038, 1076, 1090, 1146, 1409, 1497, 1523 |
| HpySE526I | ACGT | 5 cut(s) | 586, 800, 1477, 1918, 2006 |
| Hsp92I | GRCGYC | 1 cut(s) | 1664 |
| HspAI | GCGC | 4 cut(s) | 599, 673, 1739, 1748 |
| Ksp22I | TGATCA | 3 cut(s) | 898, 1395, 1609 |
| KspAI | GTTAAC | 1 cut(s) | 882 |
| LguI | GCTCTTC | 2 cut(s) | 28, 99 |
| LmnI | GCTCC | 8 cut(s) | 24, 194, 341, 1348, 1378, 1560, 1885, 1973 |
| Lsp1109I | GCAGC | 4 cut(s) | 53, 75, 760, 1338 |
| LweI | GCATC | 4 cut(s) | 219, 243, 353, 1483 |
| MaeI | CTAG | 7 cut(s) | 581, 612, 848, 1034, 1605, 1619, 1682 |
| MaeII | ACGT | 5 cut(s) | 586, 800, 1477, 1918, 2006 |
| MaeIII | GTNAC | 3 cut(s) | 694, 971, 1822 |
| MbiI | CCGCTC | 1 cut(s) | 1089 |
| MboII | GAAGA | 9 cut(s) | 45, 86, 146, 564, 1075, 1609, 1777, 1903, 1991 |
| MfeI | CAATTG | 1 cut(s) | 951 |
| MflI | RGATCY | 3 cut(s) | 542, 554, 569 |
| MhlI | GDGCHC | 1 cut(s) | 40 |
| MlyI | GAGTC | 3 cut(s) | 161, 788, 1414 |
| MmeI | TCCRAC | 3 cut(s) | 544, 802, 1234 |
| Mph1103I | ATGCAT | 2 cut(s) | 521, 1376 |
| MroXI | GAANNNNTTC | 1 cut(s) | 519 |
| MseI | TTAA | 9 cut(s) | 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1476 |
| MspA1I | CMGCKG | 2 cut(s) | 58, 66 |
| MspI | CCGG | 1 cut(s) | 266 |
| MunI | CAATTG | 1 cut(s) | 951 |
| Mva1269I | GAATGC | 3 cut(s) | 519, 521, 1362 |
| MvnI | CGCG | 3 cut(s) | 1739, 1748, 1849 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 17, 253, 262, 598, 1175, 1184, 1738, 1745, 1747 |
| NheI | GCTAGC | 1 cut(s) | 1033 |
| NlaIV | GGNNCC | 4 cut(s) | 556, 1428, 1887, 1975 |
| NsbI | TGCGCA | 1 cut(s) | 600 |
| NsiI | ATGCAT | 2 cut(s) | 521, 1376 |
| NspI | RCATGY | 4 cut(s) | 981, 1256, 1374, 1385 |
| PaeI | GCATGC | 1 cut(s) | 1385 |
| PagI | TCATGA | 1 cut(s) | 1024 |
| PaqCI | CACCTGC | 2 cut(s) | 527, 1020 |
| PciI | ACATGT | 1 cut(s) | 1252 |
| PciSI | GCTCTTC | 2 cut(s) | 28, 99 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1846 |
| PctI | GAATGC | 3 cut(s) | 519, 521, 1362 |
| PdmI | GAANNNNTTC | 1 cut(s) | 519 |
| PfeI | GAWTC | 9 cut(s) | 296, 985, 1042, 1333, 1468, 1643, 1759, 1934, 2022 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 851 |
| PkrI | GCNGC | 4 cut(s) | 65, 68, 775, 1353 |
| PleI | GAGTC | 3 cut(s) | 161, 788, 1413 |
| PpsI | GAGTC | 3 cut(s) | 161, 788, 1413 |
| Ppu21I | YACGTR | 1 cut(s) | 801 |
| PpuMI | RGGWCCY | 1 cut(s) | 1427 |
| PscI | ACATGT | 1 cut(s) | 1252 |
| PshBI | ATTAAT | 1 cut(s) | 912 |
| Psp5II | RGGWCCY | 1 cut(s) | 1427 |
| PspFI | CCCAGC | 3 cut(s) | 58, 256, 652 |
| PspN4I | GGNNCC | 4 cut(s) | 556, 1428, 1887, 1975 |
| PspPI | GGNCC | 4 cut(s) | 115, 268, 429, 1427 |
| PspPPI | RGGWCCY | 1 cut(s) | 1427 |
| PstI | CTGCAG | 1 cut(s) | 778 |
| PsuI | RGATCY | 3 cut(s) | 542, 554, 569 |
| PvuII | CAGCTG | 2 cut(s) | 58, 66 |
| RsaI | GTAC | 7 cut(s) | 51, 1001, 1488, 1624, 1736, 1928, 2016 |
| RsaNI | GTAC | 7 cut(s) | 50, 1000, 1487, 1623, 1735, 1927, 2015 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1476 |
| SapI | GCTCTTC | 2 cut(s) | 28, 99 |
| SaqAI | TTAA | 9 cut(s) | 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637 |
| SatI | GCNGC | 4 cut(s) | 64, 67, 774, 1352 |
| Sau96I | GGNCC | 4 cut(s) | 115, 268, 429, 1427 |
| SchI | GAGTC | 3 cut(s) | 161, 788, 1414 |
| SduI | GDGCHC | 1 cut(s) | 40 |
| SfaNI | GCATC | 4 cut(s) | 219, 243, 353, 1483 |
| SfcI | CTRYAG | 2 cut(s) | 384, 774 |
| SinI | GGWCC | 3 cut(s) | 268, 429, 1427 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1476 |
| SnaBI | TACGTA | 1 cut(s) | 801 |
| SphI | GCATGC | 1 cut(s) | 1385 |
| SsiI | CCGC | 8 cut(s) | 432, 440, 1087, 1629, 1847, 1863, 1880, 1968 |
| SspI | AATATT | 1 cut(s) | 1197 |
| SspMI | CTAG | 7 cut(s) | 581, 612, 848, 1034, 1605, 1619, 1682 |
| StyI | CCWWGG | 1 cut(s) | 1430 |
| TaaI | ACNGT | 4 cut(s) | 499, 820, 965, 1816 |
| TaiI | ACGT | 5 cut(s) | 589, 803, 1480, 1921, 2009 |
| TaqI | TCGA | 9 cut(s) | 351, 493, 510, 616, 1803, 1891, 1937, 1979, 2025 |
| TatI | WGTACW | 1 cut(s) | 1486 |
| TfiI | GAWTC | 9 cut(s) | 296, 985, 1042, 1333, 1468, 1643, 1759, 1934, 2022 |
| Tru1I | TTAA | 9 cut(s) | 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637 |
| Tru9I | TTAA | 9 cut(s) | 378, 413, 687, 881, 912, 1107, 1569, 1578, 1637 |
| TscAI | CASTG | 3 cut(s) | 18, 34, 739 |
| TseI | GCWGC | 4 cut(s) | 63, 66, 773, 1351 |
| TspDTI | ATGAA | 7 cut(s) | 17, 118, 459, 1013, 1041, 1460, 1544 |
| TspGWI | ACGGA | 1 cut(s) | 288 |
| TspRI | CASTG | 3 cut(s) | 18, 34, 739 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 851 |
| VpaK11BI | GGWCC | 3 cut(s) | 268, 429, 1427 |
| VspI | ATTAAT | 1 cut(s) | 912 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1343 |
| XapI | RAATTY | 2 cut(s) | 5, 1139 |
| XbaI | TCTAGA | 1 cut(s) | 580 |
| XceI | RCATGY | 4 cut(s) | 981, 1256, 1374, 1385 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 818 |
| XmnI | GAANNNNTTC | 1 cut(s) | 519 |
| XspI | CTAG | 7 cut(s) | 581, 612, 848, 1034, 1605, 1619, 1682 |
| Zsp2I | ATGCAT | 2 cut(s) | 521, 1376 |