Rorug07G0201000
ERF Family

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006935
Physical Location & Seq
Reverse (-)
17456734 .. 17461201
4468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0201000.1

Sequence Viewer

Length: 1659 bp
ATGCCCCTCCCTCTCTCCCCTCGGCCCCGCAATCGCAACAAACACAATACCTCTCCGTCCGTTGATCCCCTTCCGCCTTCTCTCACCTTTTTCCGTTCAAACGCCCAAACTCCTCCGGCCCAGAAATCCCAAAGCCCCAATCTTTCTTCCCCTACAAAAATGGACCATCACCACCTCCAACAGCTTCAGCAACAGCAGCTCCTCCAAGCCCAACACCAACACCAACACCAACAACAACACTTCCAACCCCCACACTACCATTACAATACCAATACCCACCAATCTTACTACCCTCCTCAATATCAATACCCACAACAACAACAACAACTACAACAAAATGGTTATCACCCATCAAACTATACCAATCTCTATCCCCAGCAGGCTTATCCCCAGTCGGCAGTGCCTCGACCACCTGAGCTTCCGGTCCCGCCAGCGCTGGCTGTGATGTCTCGTTTGGTGCAATACCCCGGGGCAGTGGGGTCTCAATGGAACACTCCACCAATGGTTGGCCAACCTCCATATAGGGGTATCGGTGAAGTGGGTGGTGAACTATTCAGAGGACATCAAGGAAATTTTGGCTACAGCGGAGCTCACCCATCTTTCATTGGGGGATCACATGGTCGAGGTAGGGGTGGTTCTTGGCTTTTAGTGGAACATGGTGTCCCTTCAACATCTTCTCATCCTCCCCATCAGACATACCAATCTCCGGTAACGCTGACACCTCAGAAGTCGGAATCTGCATCAGCACTGAAAACGGAAGTTCCAAACTACCAAAAACCTCAAGTTGCTCAAAAAGCCACGGTTACTTGGTGTGAAGTTTGTAATGCTTCATGCGCTAATTTGGACGTTCTTAAACAGCACCAAAATGGAAAGCGACATCAAAGGAACCTGAGAAAAATGGAAAGGTTGAAAAATGGTATCAGAACAGTGGCCAAATTGCAGGGCCAACAGCTTTGCACTTCTAATACAAAGCTTGAAGTGCCTCATCAGATTCAGTGTGAACAAGACGATGAAGAGAAAAACACAACAAAGGATATACCATACGAACCTGTTGGCTATGAAAATTCAAGGGAAACTGGAAAACAAATTAAGAACCCCGGACAGCCTGAAATTCCACCAGGTGATGGGTCTGACAATGTGAAGCGTGGTAAGAAGCGCAAGAAAACGAAAACTCCGAAGCAGTCAGTCAAACCTTCCAAATGTAAAGTTGTAATTCCGATAGTTTGTGACTTGTGTAATGTCAAGTGTGACAAGCAGGCTTTTTTCGATCGCCATCTTTCAGGAAAGAAGCATGTTGCCAAGTATAAACGATTTAAAGGCCATGAAGCTATGCATCAACCCGTGGGGCTTCAATTTCTATACCCCCCTAACCCATTGGCTGAAACTATATTCCAACCCCAAGAAGGAAGATATATACCTCCCCATGTGTACCAAGATATACAGGTGGAAACAAGTTCAGATCCCCAATTTCAACAAAATCCAACCACTGAAGGATCTGAATCCATTCTTGGATGTGGAAGCTCTAGTACCATTACAACAGCTCTAGAAACCAACATCACAACAGAACCGGAAATCAGCCAAGAAGCTACCATTGAGGTCCAACATTTGAATGGTATTTCAGAGACTGGTCAGGGGTCCAATACTTTTTCACAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

552

Amino Acids

61.77

Weight (kDa)

9.12

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 270 - 293 7e-07 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 506, 1124
AciI CCGC 4 cut(s) 28, 74, 428, 585
AclWI GGATC 4 cut(s) 59, 619, 1454, 1501
AcoI YGGCCR 2 cut(s) 508, 930
AcsI RAATTY 3 cut(s) 571, 1063, 1110
AcuI CTGAAG 2 cut(s) 170, 1509
AdeI CACNNNGTG 1 cut(s) 1121
AfaI GTAC 2 cut(s) 1430, 1528
AfeI AGCGCT 1 cut(s) 435
AfiI CCNNNNNNNGG 5 cut(s) 506, 524, 706, 1124, 1403
AgsI TTSAA 8 cut(s) 99, 669, 910, 977, 1068, 1352, 1472, 1609
AjnI CCWGG 1 cut(s) 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 1491, 1523
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 3 cut(s) 453, 486, 1616
AlwI GGATC 4 cut(s) 59, 619, 1454, 1501
AlwNI CAGNNNCTG 1 cut(s) 1625
Ama87I CYCGRG 1 cut(s) 467
Aor51HI AGCGCT 1 cut(s) 435
AoxI GGCC 6 cut(s) 23, 117, 508, 930, 943, 1318
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 3 cut(s) 571, 1063, 1110
Asp700I GAANNNNTTC 1 cut(s) 1503
AspLEI GCGC 3 cut(s) 436, 836, 1158
AspS9I GGNCC 7 cut(s) 24, 118, 163, 424, 943, 1597, 1635
AsuC2I CCSGG 3 cut(s) 468, 469, 1098
AsuHPI GGTGA 7 cut(s) 76, 161, 338, 545, 557, 584, 1133
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 4 cut(s) 163, 424, 1597, 1635
BalI TGGCCA 2 cut(s) 510, 932
BanII GRGCYC 1 cut(s) 592
BarI GAAGNNNNNNTAC 2 cut(s) 1510, 1542
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 1 cut(s) 208
BccI CCATC 6 cut(s) 174, 358, 604, 696, 1118, 1281
BciT130I CCWGG 1 cut(s) 1119
BcnI CCSGG 3 cut(s) 468, 469, 1098
BcoDI GTCTC 3 cut(s) 453, 486, 1616
BfaI CTAG 2 cut(s) 1524, 1544
BfmI CTRYAG 1 cut(s) 580
BfoI RGCGCY 1 cut(s) 437
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
Bme1390I CCNGG 4 cut(s) 468, 469, 1098, 1119
Bme18I GGWCC 4 cut(s) 163, 424, 1597, 1635
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 7 cut(s) 24, 118, 163, 424, 943, 1597, 1635
BmiI GGNNCC 4 cut(s) 26, 426, 887, 1636
BmrFI CCNGG 4 cut(s) 468, 469, 1098, 1119
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 2 cut(s) 749, 1342
BmuI ACTGGG 1 cut(s) 385
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 3 cut(s) 468, 469, 1098
BsaBI GATNNNNATC 2 cut(s) 1272, 1498
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 7 cut(s) 20, 466, 467, 468, 798, 1096, 1341
BsaWI WCCGGW 3 cut(s) 421, 706, 1567
BsaXI ACNNNNNCTCC 4 cut(s) 183, 213, 1156, 1186
Bsc4I CCNNNNNNNGG 5 cut(s) 506, 524, 706, 1124, 1403
Bse1I ACTGG 3 cut(s) 391, 1081, 1630
Bse8I GATNNNNATC 2 cut(s) 1272, 1498
BseBI CCWGG 1 cut(s) 1119
BseDI CCNNGG 7 cut(s) 20, 466, 467, 468, 798, 1096, 1341
BseGI GGATG 2 cut(s) 679, 1517
BseJI GATNNNNATC 2 cut(s) 1272, 1498
BseLI CCNNNNNNNGG 5 cut(s) 506, 524, 706, 1124, 1403
BseMII CTCAG 3 cut(s) 405, 737, 881
BseNI ACTGG 3 cut(s) 391, 1081, 1630
BseRI GAGGAG 3 cut(s) 102, 191, 285
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 1270
BshFI GGCC 6 cut(s) 25, 119, 510, 932, 945, 1320
BsiEI CGRYCG 1 cut(s) 1270
BsiHKAI GWGCWC 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 6 cut(s) 116, 422, 468, 707, 1098, 1568
BslFI GGGAC 2 cut(s) 410, 647
BslI CCNNNNNNNGG 5 cut(s) 506, 524, 706, 1124, 1403
BsmAI GTCTC 3 cut(s) 453, 486, 1616
BsmFI GGGAC 2 cut(s) 410, 647
BsnI GGCC 6 cut(s) 25, 119, 510, 932, 945, 1320
Bso31I GGTCTC 1 cut(s) 486
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 5 cut(s) 64, 611, 1267, 1459, 1493
BspACI CCGC 4 cut(s) 28, 74, 428, 585
BspANI GGCC 6 cut(s) 25, 119, 510, 932, 945, 1320
BspCNI CTCAG 3 cut(s) 406, 736, 882
BspLI GGNNCC 4 cut(s) 26, 426, 887, 1636
BspPI GGATC 4 cut(s) 59, 619, 1454, 1501
BspTNI GGTCTC 1 cut(s) 486
BsrI ACTGG 3 cut(s) 391, 1081, 1630
BssECI CCNNGG 7 cut(s) 20, 466, 467, 468, 798, 1096, 1341
BssMI GATC 5 cut(s) 64, 611, 1267, 1459, 1493
Bst2UI CCWGG 1 cut(s) 1119
Bst4CI ACNGT 2 cut(s) 802, 928
Bst6I CTCTTC 1 cut(s) 1008
BstC8I GCNNGC 4 cut(s) 381, 432, 438, 1257
BstDEI CTNAG 3 cut(s) 414, 723, 890
BstDSI CCRYGG 2 cut(s) 798, 1341
BstF5I GGATG 2 cut(s) 679, 1517
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 3 cut(s) 436, 836, 1158
BstKTI GATC 5 cut(s) 67, 614, 1270, 1462, 1496
BstMAI GTCTC 3 cut(s) 453, 486, 1616
BstMBI GATC 5 cut(s) 64, 611, 1267, 1459, 1493
BstMCI CGRYCG 1 cut(s) 1270
BstMWI GCNNNNNNNGC 4 cut(s) 196, 794, 833, 979
BstNI CCWGG 1 cut(s) 1119
BstNSI RCATGY 1 cut(s) 1295
BstSCI CCNGG 4 cut(s) 466, 467, 1096, 1117
BstSFI CTRYAG 1 cut(s) 580
BstV1I GCAGC 1 cut(s) 208
BstX2I RGATCY 2 cut(s) 1459, 1493
BstYI RGATCY 2 cut(s) 1459, 1493
BsuRI GGCC 6 cut(s) 25, 119, 510, 932, 945, 1320
BtgI CCRYGG 2 cut(s) 798, 1341
BtsCI GGATG 2 cut(s) 679, 1517
BtsI GCAGTG 2 cut(s) 405, 480
BtsIMutI CAGTG 6 cut(s) 405, 480, 746, 933, 1001, 1485
Cac8I GCNNGC 4 cut(s) 381, 432, 438, 1257
CaiI CAGNNNCTG 1 cut(s) 1625
CfoI GCGC 3 cut(s) 436, 836, 1158
Cfr13I GGNCC 7 cut(s) 24, 118, 163, 424, 943, 1597, 1635
Cfr9I CCCGGG 1 cut(s) 467
CsiI ACCWGGT 1 cut(s) 1117
Csp6I GTAC 2 cut(s) 1429, 1527
CviAII CATG 6 cut(s) 617, 656, 831, 1292, 1322, 1424
CviQI GTAC 2 cut(s) 1429, 1527
DdeI CTNAG 3 cut(s) 414, 723, 890
DpnI GATC 5 cut(s) 66, 613, 1269, 1461, 1495
DpnII GATC 5 cut(s) 64, 611, 1267, 1459, 1493
DraI TTTAAA 1 cut(s) 1315
DraIII CACNNNGTG 1 cut(s) 1121
EaeI YGGCCR 2 cut(s) 508, 930
Eam1104I CTCTTC 1 cut(s) 1008
EarI CTCTTC 1 cut(s) 1008
EciI GGCGGA 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 592
Eco31I GGTCTC 1 cut(s) 486
Eco47I GGWCC 4 cut(s) 163, 424, 1597, 1635
Eco47III AGCGCT 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 2 cut(s) 170, 1509
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 1117
EcoT22I ATGCAT 1 cut(s) 1335
EcoT38I GRGCYC 1 cut(s) 592
FaeI CATG 6 cut(s) 620, 659, 834, 1295, 1325, 1427
FaqI GGGAC 2 cut(s) 410, 647
FatI CATG 6 cut(s) 616, 655, 830, 1291, 1321, 1423
FauI CCCGC 2 cut(s) 35, 435
Fnu4HI GCNGC 1 cut(s) 197
FokI GGATG 2 cut(s) 666, 1524
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 2 cut(s) 1524, 1544
GlaI GCGC 3 cut(s) 435, 835, 1157
GluI GCNGC 1 cut(s) 197
GsaI CCCAGC 1 cut(s) 379
HaeII RGCGCY 1 cut(s) 437
HaeIII GGCC 6 cut(s) 25, 119, 510, 932, 945, 1320
HapII CCGG 6 cut(s) 116, 422, 468, 707, 1098, 1568
HhaI GCGC 3 cut(s) 436, 836, 1158
Hin1II CATG 6 cut(s) 620, 659, 834, 1295, 1325, 1427
Hin6I GCGC 3 cut(s) 434, 834, 1156
HinP1I GCGC 3 cut(s) 434, 834, 1156
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 3 cut(s) 734, 991, 1499
HpaII CCGG 6 cut(s) 116, 422, 468, 707, 1098, 1568
HphI GGTGA 7 cut(s) 76, 161, 338, 545, 557, 584, 1133
Hpy166II GTNNAC 3 cut(s) 548, 1001, 1429
Hpy188III TCNNGA 2 cut(s) 1281, 1544
Hpy8I GTNNAC 3 cut(s) 548, 1001, 1429
HpyAV CCTTC 6 cut(s) 80, 87, 675, 1203, 1397, 1484
HpyCH4III ACNGT 2 cut(s) 802, 928
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 5 cut(s) 460, 740, 940, 957, 1333
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 794, 833, 979
HpyF3I CTNAG 3 cut(s) 414, 723, 890
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 6 cut(s) 620, 659, 834, 1295, 1325, 1427
HspAI GCGC 3 cut(s) 434, 834, 1156
Kzo9I GATC 5 cut(s) 64, 611, 1267, 1459, 1493
LmnI GCTCC 2 cut(s) 204, 587
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 2 cut(s) 749, 1342
MabI ACCWGGT 1 cut(s) 1117
MaeI CTAG 2 cut(s) 1524, 1544
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 4 cut(s) 709, 802, 1226, 1247
MalI GATC 5 cut(s) 66, 613, 1269, 1461, 1495
MboI GATC 5 cut(s) 64, 611, 1267, 1459, 1493
MboII GAAGA 4 cut(s) 138, 666, 1025, 1419
MflI RGATCY 2 cut(s) 1459, 1493
MhlI GDGCHC 1 cut(s) 592
MlsI TGGCCA 2 cut(s) 510, 932
MluCI AATT 9 cut(s) 571, 838, 935, 1063, 1086, 1110, 1212, 1352, 1466
MluNI TGGCCA 2 cut(s) 510, 932
MmeI TCCRAC 6 cut(s) 202, 268, 711, 1417, 1505, 1624
Mox20I TGGCCA 2 cut(s) 510, 932
Mph1103I ATGCAT 1 cut(s) 1335
MroXI GAANNNNTTC 1 cut(s) 1503
MscI TGGCCA 2 cut(s) 510, 932
MseI TTAA 3 cut(s) 852, 1089, 1314
MslI CAYNNNNRTG 2 cut(s) 864, 1608
Msp20I TGGCCA 2 cut(s) 510, 932
MspA1I CMGCKG 1 cut(s) 585
MspI CCGG 6 cut(s) 116, 422, 468, 707, 1098, 1568
MspR9I CCNGG 4 cut(s) 468, 469, 1098, 1119
MvaI CCWGG 1 cut(s) 1119
MwoI GCNNNNNNNGC 4 cut(s) 196, 794, 833, 979
NciI CCSGG 3 cut(s) 468, 469, 1098
NdeII GATC 5 cut(s) 64, 611, 1267, 1459, 1493
NlaIII CATG 6 cut(s) 620, 659, 834, 1295, 1325, 1427
NlaIV GGNNCC 4 cut(s) 26, 426, 887, 1636
NmuCI GTSAC 2 cut(s) 1226, 1247
NsiI ATGCAT 1 cut(s) 1335
NspI RCATGY 1 cut(s) 1295
PcsI WCGNNNNNNNCGW 1 cut(s) 1172
PdmI GAANNNNTTC 1 cut(s) 1503
PfeI GAWTC 3 cut(s) 734, 991, 1499
PflMI CCANNNNNTGG 2 cut(s) 506, 1124
PkrI GCNGC 1 cut(s) 198
Ple19I CGATCG 1 cut(s) 1270
Psp124BI GAGCTC 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 1117
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 1 cut(s) 1117
PspN4I GGNNCC 4 cut(s) 26, 426, 887, 1636
PspPI GGNCC 7 cut(s) 24, 118, 163, 424, 943, 1597, 1635
PsrI GAACNNNNNNTAC 2 cut(s) 619, 651
PstNI CAGNNNCTG 1 cut(s) 1625
PsuI RGATCY 2 cut(s) 1459, 1493
PvuI CGATCG 1 cut(s) 1270
RsaI GTAC 2 cut(s) 1430, 1528
RsaNI GTAC 2 cut(s) 1429, 1527
RseI CAYNNNNRTG 2 cut(s) 864, 1608
SacI GAGCTC 1 cut(s) 592
SaqAI TTAA 3 cut(s) 852, 1089, 1314
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 5 cut(s) 64, 611, 1267, 1459, 1493
Sau96I GGNCC 7 cut(s) 24, 118, 163, 424, 943, 1597, 1635
ScrFI CCNGG 4 cut(s) 468, 469, 1098, 1119
SduI GDGCHC 1 cut(s) 592
SexAI ACCWGGT 1 cut(s) 1117
SfaNI GCATC 2 cut(s) 749, 1342
SfcI CTRYAG 1 cut(s) 580
SinI GGWCC 4 cut(s) 163, 424, 1597, 1635
SmaI CCCGGG 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 864, 1608
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
Sse9I AATT 9 cut(s) 571, 838, 935, 1063, 1086, 1110, 1212, 1352, 1466
SsiI CCGC 4 cut(s) 28, 74, 428, 585
SspMI CTAG 2 cut(s) 1524, 1544
SstI GAGCTC 1 cut(s) 592
StyD4I CCNGG 4 cut(s) 466, 467, 1096, 1117
TaaI ACNGT 2 cut(s) 802, 928
TaiI ACGT 1 cut(s) 849
TaqI TCGA 3 cut(s) 406, 622, 1266
TasI AATT 9 cut(s) 571, 838, 935, 1063, 1086, 1110, 1212, 1352, 1466
TfiI GAWTC 3 cut(s) 734, 991, 1499
Tru1I TTAA 3 cut(s) 852, 1089, 1314
Tru9I TTAA 3 cut(s) 852, 1089, 1314
TscAI CASTG 6 cut(s) 405, 480, 753, 933, 1001, 1492
TseFI GTSAC 2 cut(s) 1226, 1247
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 2 cut(s) 1226, 1247
TspDTI ATGAA 5 cut(s) 592, 819, 1026, 1074, 1338
TspGWI ACGGA 4 cut(s) 45, 49, 83, 770
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 6 cut(s) 405, 480, 753, 933, 1001, 1492
Van91I CCANNNNNTGG 2 cut(s) 506, 1124
VpaK11BI GGWCC 4 cut(s) 163, 424, 1597, 1635
XapI RAATTY 3 cut(s) 571, 1063, 1110
XbaI TCTAGA 1 cut(s) 1543
XceI RCATGY 1 cut(s) 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1607
XmaI CCCGGG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 1503
XspI CTAG 2 cut(s) 1524, 1544
Zsp2I ATGCAT 1 cut(s) 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.