RLG00000001455

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006935
Physical Location & Seq
Forward (+)
15785680 .. 15791995
6316 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001455

Sequence Viewer

Length: 1200 bp
ATGAAATTCGCAGTGAAGGCATGGAGCAGTGGAAGAGCACGGGCAGGGGTACTCCAGCTGGGCAGCAGCCCGAACCCAATAGAGACCCCTGCTCTTCTCCTCTCTACCCGAAAGGGCCTGCCCCTTTTCATACCCCCTGACCTTCTCCCTTCTCTTCCTTCCCCTGACTCCCATCTCCTCCATGTTAGCTCCCTTCACTTCTTAGAAGGCATCTCGCCGCAGACAATATCAAAAATAGGAGGATTGCATAAGATGCTGGGTTTGCCGGACCACGAATTCGTAGCCATTCCGAGAGATTCTATTCAATGCCTCCCAGAATGTAATAGTACCAATAAATTGGGAGCATCTTTTGAGACTCCTTGTGGTCGCTGTTTGGTAAAGCCTGTAGAGTATGTTGAATTGATTTCTTCGTTAAAGCCTAATATATGGACTGCTTTGGCGGATGAGGTCCCTGCATGGGTGTCTGATAAGAGGAACAAGACTTCGGTTGATCGAACAGTAAGATGGCTTGACGAATGTGTTCAGCTAAATCGGGCAGGTGGATCTGTTTTTGGATCCATTGTTGGAGGATCTTCTGTTCAAGAACGTGAGCGATGTGCACAAGAGGTGGCCAGCCGAAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATGGATGAGCGCCCTGCTCTACTTAATGCTGTTACTGATATCTTACAAGAGGAGAAACCACGCTTGGTCAGTGGGCTTGGACTTCCAGAAGAGGTCTTGCAGGGTGTTGCTGCAGGAATTGATATTTTTGACTCTACGTATATATACCATCTTACCCTTGGAGGCTTTGCACTTACCTTCCCACTAGATGGTGTTGAGACCAATCTCTCTGCTTTTCAGTTAACTAAAATAGGAAGTGATCAGACAAAGATTAATTTGAGAGCAACAATGTACAGGAAAGACACTTCACCAATTGTTGAAAATTGTAAATGTTACACGTGCCAGAATCATACAAAAGCGTACATCAATCACCTGCTCAATGTTCATGAAATGCTAGCTCAGATTCTACTTGAAATACATAATACTCACCACTATCTAAGTTTCTTCCGCTCAGTAAGGAAGGCAATTAAGTCCGGTAATTTTGAGCAATTCAGACTGAAATTTTCTGAGAGTAAGCGTTATCATCTTGCTTCTGAAGCTGCTGTTGCCTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

400

Amino Acids

43.96

Weight (kDa)

7.19

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 12 - 385 1.3e-89 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 1023
Acc36I ACCTGC 2 cut(s) 527, 1023
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 1 cut(s) 1092
AciI CCGC 3 cut(s) 218, 440, 1090
AclWI GGATC 4 cut(s) 549, 550, 562, 577
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 3 cut(s) 5, 275, 1142
AcuI CTGAAG 1 cut(s) 1197
AcvI CACGTG 1 cut(s) 981
AfaI GTAC 4 cut(s) 51, 328, 935, 1004
AfiI CCNNNNNNNGG 2 cut(s) 457, 851
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 5 cut(s) 305, 398, 581, 962, 1055
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 5 cut(s) 58, 189, 526, 1040, 1181
AluI AGCT 5 cut(s) 58, 189, 526, 1040, 1181
Alw21I GWGCWC 2 cut(s) 40, 601
Alw26I GTCTC 3 cut(s) 77, 347, 854
Alw44I GTGCAC 1 cut(s) 597
AlwI GGATC 4 cut(s) 549, 550, 562, 577
AoxI GGCC 2 cut(s) 115, 609
ApaLI GTGCAC 1 cut(s) 597
ApeKI GCWGC 4 cut(s) 63, 66, 773, 1181
ApoI RAATTY 3 cut(s) 5, 275, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AseI ATTAAT 1 cut(s) 915
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 3 cut(s) 115, 268, 448
AsuHPI GGTGA 3 cut(s) 942, 1004, 1061
AsuNHI GCTAGC 1 cut(s) 1036
AvaII GGWCC 2 cut(s) 268, 448
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 554
BbrPI CACGTG 1 cut(s) 981
Bbv12I GWGCWC 2 cut(s) 40, 601
BbvI GCAGC 4 cut(s) 75, 78, 760, 1168
BccI CCATC 4 cut(s) 180, 498, 819, 845
BclI TGATCA 1 cut(s) 901
BcoDI GTCTC 3 cut(s) 77, 347, 854
BfaI CTAG 2 cut(s) 848, 1037
BfmI CTRYAG 2 cut(s) 384, 774
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 527, 1023
BisI GCNGC 5 cut(s) 64, 67, 218, 774, 1182
BlsI GCNGC 5 cut(s) 65, 68, 219, 775, 1183
Bme18I GGWCC 2 cut(s) 268, 448
BmgT120I GGNCC 3 cut(s) 115, 268, 448
BmiI GGNNCC 2 cut(s) 450, 556
BmsI GCATC 3 cut(s) 219, 243, 353
BmtI GCTAGC 1 cut(s) 1040
BpmI CTGGAG 1 cut(s) 38
BsaAI YACGTR 2 cut(s) 801, 981
BsaI GGTCTC 2 cut(s) 77, 854
BsaJI CCNNGG 1 cut(s) 820
BsaWI WCCGGW 1 cut(s) 1115
Bsc4I CCNNNNNNNGG 2 cut(s) 457, 851
BseDI CCNNGG 1 cut(s) 820
BseGI GGATG 2 cut(s) 448, 673
BseLI CCNNNNNNNGG 2 cut(s) 457, 851
BseMII CTCAG 3 cut(s) 1055, 1107, 1140
BseRI GAGGAG 3 cut(s) 89, 167, 728
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 4 cut(s) 75, 78, 760, 1168
BseYI CCCAGC 2 cut(s) 58, 256
BshFI GGCC 2 cut(s) 117, 611
BsiHKAI GWGCWC 2 cut(s) 40, 601
BsiSI CCGG 2 cut(s) 266, 1116
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 2 cut(s) 457, 851
BsmAI GTCTC 3 cut(s) 77, 347, 854
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 2 cut(s) 117, 611
Bso31I GGTCTC 2 cut(s) 77, 854
Bsp1286I GDGCHC 2 cut(s) 40, 601
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 5 cut(s) 490, 542, 554, 569, 901
BspACI CCGC 3 cut(s) 218, 440, 1090
BspANI GGCC 2 cut(s) 117, 611
BspCNI CTCAG 3 cut(s) 1054, 1106, 1141
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 2 cut(s) 450, 556
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 527, 1023
BspOI GCTAGC 1 cut(s) 1040
BspPI GGATC 4 cut(s) 549, 550, 562, 577
BspQI GCTCTTC 2 cut(s) 28, 99
BspTNI GGTCTC 2 cut(s) 77, 854
BsrBI CCGCTC 1 cut(s) 1092
BsrGI TGTACA 1 cut(s) 933
BssECI CCNNGG 1 cut(s) 820
BssMI GATC 5 cut(s) 490, 542, 554, 569, 901
BssT1I CCWWGG 1 cut(s) 820
Bst4CI ACNGT 1 cut(s) 499
Bst6I CTCTTC 4 cut(s) 28, 99, 159, 747
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 933
BstBAI YACGTR 2 cut(s) 801, 981
BstC8I GCNNGC 4 cut(s) 119, 613, 1038, 1192
BstDEI CTNAG 5 cut(s) 202, 1041, 1079, 1093, 1149
BstF5I GGATG 2 cut(s) 448, 673
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 5 cut(s) 493, 545, 557, 572, 904
BstMAI GTCTC 3 cut(s) 77, 347, 854
BstMBI GATC 5 cut(s) 490, 542, 554, 569, 901
BstMWI GCNNNNNNNGC 5 cut(s) 17, 253, 262, 1178, 1187
BstNSI RCATGY 1 cut(s) 1198
BstSFI CTRYAG 2 cut(s) 384, 774
BstSLI GKGCMC 1 cut(s) 601
BstSNI TACGTA 1 cut(s) 801
BstV1I GCAGC 4 cut(s) 75, 78, 760, 1168
BstX2I RGATCY 3 cut(s) 542, 554, 569
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 542, 554, 569
BsuRI GGCC 2 cut(s) 117, 611
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 448, 673
BtsI GCAGTG 2 cut(s) 18, 34
BtsIMutI CAGTG 3 cut(s) 18, 34, 739
BveI ACCTGC 2 cut(s) 527, 1023
Cac8I GCNNGC 4 cut(s) 119, 613, 1038, 1192
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 1 cut(s) 675
Cfr13I GGNCC 3 cut(s) 115, 268, 448
Csp6I GTAC 4 cut(s) 50, 327, 934, 1003
CviAII CATG 6 cut(s) 21, 182, 456, 664, 1028, 1195
CviQI GTAC 4 cut(s) 50, 327, 934, 1003
DdeI CTNAG 5 cut(s) 202, 1041, 1079, 1093, 1149
DpnI GATC 5 cut(s) 492, 544, 556, 571, 903
DpnII GATC 5 cut(s) 490, 542, 554, 569, 901
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 4 cut(s) 28, 99, 159, 747
EarI CTCTTC 4 cut(s) 28, 99, 159, 747
EciI GGCGGA 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 801
Eco130I CCWWGG 1 cut(s) 820
Eco31I GGTCTC 2 cut(s) 77, 854
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 268, 448
Eco57I CTGAAG 1 cut(s) 1197
Eco72I CACGTG 1 cut(s) 981
EcoO109I RGGNCCY 2 cut(s) 115, 448
EcoRI GAATTC 1 cut(s) 275
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 820
ErhI CCWWGG 1 cut(s) 820
FaeI CATG 6 cut(s) 24, 185, 459, 667, 1031, 1198
FaqI GGGAC 1 cut(s) 434
FatI CATG 6 cut(s) 20, 181, 455, 663, 1027, 1194
FbaI TGATCA 1 cut(s) 901
Fnu4HI GCNGC 5 cut(s) 64, 67, 218, 774, 1182
FokI GGATG 2 cut(s) 455, 680
Fsp4HI GCNGC 5 cut(s) 64, 67, 218, 774, 1182
FspBI CTAG 2 cut(s) 848, 1037
GlaI GCGC 1 cut(s) 674
GluI GCNGC 5 cut(s) 64, 67, 218, 774, 1182
GsaI CCCAGC 2 cut(s) 62, 260
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 2 cut(s) 117, 611
HapII CCGG 2 cut(s) 266, 1116
HhaI GCGC 1 cut(s) 675
Hin1II CATG 6 cut(s) 24, 185, 459, 667, 1031, 1198
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HinfI GANTC 6 cut(s) 167, 296, 355, 794, 988, 1045
HpaI GTTAAC 1 cut(s) 885
HpaII CCGG 2 cut(s) 266, 1116
HphI GGTGA 3 cut(s) 942, 1004, 1061
Hpy166II GTNNAC 2 cut(s) 599, 885
Hpy188I TCNGA 7 cut(s) 291, 466, 906, 1044, 1136, 1150, 1177
Hpy188III TCNNGA 3 cut(s) 581, 749, 1028
Hpy8I GTNNAC 2 cut(s) 599, 885
HpyAV CCTTC 8 cut(s) 10, 152, 159, 168, 200, 203, 850, 1096
HpyCH4III ACNGT 1 cut(s) 499
HpyCH4IV ACGT 3 cut(s) 586, 800, 980
HpyCH4V TGCA 7 cut(s) 247, 455, 599, 763, 776, 833, 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 253, 262, 1178, 1187
HpyF3I CTNAG 5 cut(s) 202, 1041, 1079, 1093, 1149
HpySE526I ACGT 3 cut(s) 586, 800, 980
Hsp92II CATG 6 cut(s) 24, 185, 459, 667, 1031, 1198
HspAI GCGC 1 cut(s) 673
Ksp22I TGATCA 1 cut(s) 901
KspAI GTTAAC 1 cut(s) 885
Kzo9I GATC 5 cut(s) 490, 542, 554, 569, 901
LguI GCTCTTC 2 cut(s) 28, 99
LmnI GCTCC 3 cut(s) 24, 194, 341
Lsp1109I GCAGC 4 cut(s) 75, 78, 760, 1168
LweI GCATC 3 cut(s) 219, 243, 353
MaeI CTAG 2 cut(s) 848, 1037
MaeII ACGT 3 cut(s) 586, 800, 980
MaeIII GTNAC 2 cut(s) 694, 974
MalI GATC 5 cut(s) 492, 544, 556, 571, 903
MbiI CCGCTC 1 cut(s) 1092
MboI GATC 5 cut(s) 490, 542, 554, 569, 901
MboII GAAGA 7 cut(s) 45, 86, 146, 399, 564, 764, 1078
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 3 cut(s) 542, 554, 569
MhlI GDGCHC 2 cut(s) 40, 601
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 3 cut(s) 161, 349, 788
MmeI TCCRAC 1 cut(s) 544
Mox20I TGGCCA 1 cut(s) 611
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 611
MseI TTAA 5 cut(s) 413, 687, 884, 915, 1110
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 2 cut(s) 266, 1116
MunI CAATTG 1 cut(s) 954
MwoI GCNNNNNNNGC 5 cut(s) 17, 253, 262, 1178, 1187
NdeII GATC 5 cut(s) 490, 542, 554, 569, 901
NheI GCTAGC 1 cut(s) 1036
NlaIII CATG 6 cut(s) 24, 185, 459, 667, 1031, 1198
NlaIV GGNNCC 2 cut(s) 450, 556
NspI RCATGY 1 cut(s) 1198
PagI TCATGA 1 cut(s) 1027
PaqCI CACCTGC 2 cut(s) 527, 1023
PciSI GCTCTTC 2 cut(s) 28, 99
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 3 cut(s) 296, 988, 1045
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 5 cut(s) 65, 68, 219, 775, 1183
PleI GAGTC 3 cut(s) 161, 349, 788
PmaCI CACGTG 1 cut(s) 981
PmlI CACGTG 1 cut(s) 981
PpsI GAGTC 3 cut(s) 161, 349, 788
Ppu21I YACGTR 2 cut(s) 801, 981
PpuMI RGGWCCY 1 cut(s) 448
PshBI ATTAAT 1 cut(s) 915
Psp5II RGGWCCY 1 cut(s) 448
PspCI CACGTG 1 cut(s) 981
PspFI CCCAGC 2 cut(s) 58, 256
PspN4I GGNNCC 2 cut(s) 450, 556
PspPI GGNCC 3 cut(s) 115, 268, 448
PspPPI RGGWCCY 1 cut(s) 448
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 3 cut(s) 542, 554, 569
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 4 cut(s) 51, 328, 935, 1004
RsaNI GTAC 4 cut(s) 50, 327, 934, 1003
SapI GCTCTTC 2 cut(s) 28, 99
SaqAI TTAA 5 cut(s) 413, 687, 884, 915, 1110
SatI GCNGC 5 cut(s) 64, 67, 218, 774, 1182
Sau3AI GATC 5 cut(s) 490, 542, 554, 569, 901
Sau96I GGNCC 3 cut(s) 115, 268, 448
SchI GAGTC 3 cut(s) 161, 349, 788
SduI GDGCHC 2 cut(s) 40, 601
SfaNI GCATC 3 cut(s) 219, 243, 353
SfcI CTRYAG 2 cut(s) 384, 774
SinI GGWCC 2 cut(s) 268, 448
SnaBI TACGTA 1 cut(s) 801
SsiI CCGC 3 cut(s) 218, 440, 1090
SspMI CTAG 2 cut(s) 848, 1037
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 1 cut(s) 499
TaiI ACGT 3 cut(s) 589, 803, 983
TaqI TCGA 1 cut(s) 493
TatI WGTACW 1 cut(s) 933
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 3 cut(s) 296, 988, 1045
Tru1I TTAA 5 cut(s) 413, 687, 884, 915, 1110
Tru9I TTAA 5 cut(s) 413, 687, 884, 915, 1110
TscAI CASTG 3 cut(s) 18, 34, 739
TseI GCWGC 4 cut(s) 63, 66, 773, 1181
TspDTI ATGAA 4 cut(s) 17, 118, 1016, 1044
TspRI CASTG 3 cut(s) 18, 34, 739
Van91I CCANNNNNTGG 1 cut(s) 851
VneI GTGCAC 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 268, 448
VspI ATTAAT 1 cut(s) 915
XapI RAATTY 3 cut(s) 5, 275, 1142
XceI RCATGY 1 cut(s) 1198
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 848, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.