FvH4_6g05851

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010577
Physical Location & Seq
Reverse (-)
3333223 .. 3334344
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05851.t1

Sequence Viewer

Length: 876 bp
ATGAGAACTTGCGCATCGAGGGCTTATTGCGCTTCAGGCACTTGTGCTTCTTCACTCCGCGAGTGGCTGGAATCGACTCGCTGGAGGCTTGCTTGCTTTGGGATACTTGACACTGCTGCTCTCCTGATTCATAACAGTTTGCTACTTCTATGCAGTTACGGCAAGATCTTGATCGACTACGAGGCTCTCAACCTCAGGATCGAACAAGAACAACACATCAGAGATGAATCATCGGCGTACGATTTGCTCGACTATTTCTTCAAGCTCATCCTCAACGATCGTCAAAGAAACCTAGCTGAAGCAGTTTCATGTCTTGTCTTTGCTTCTTCAAGATTAACATGCGGGGTGATTCCTAAGCTTGGGGAGATCAGGGACTTGTTCGGCGAGCGTTACGAGGAGAGTTTTGTCGACACTGCTGTTAAACTGTTGCCTGGGAATCTTGTAAACCCTACCTTCAAAAAGAATATCATCGCTCTCTGCCCGCCAGACTCAGAGGTGCCTACTGACGTCATAGTAAAACATGAACTGGTCAAACCCTCTTTTACTAGTTCTTCTCCTGATCAGGCACCAGCGAGATCATCAGAACAGAAAAAGGATGACAAGGTCCAATGCCCTAAACATGTCCACCCCAAATTGCCTGATTGGGATGATATATATACCCAGTTCAATGCTCTCAAGAAGCCTAAATATGTGAACCCTAAGTTGCCCGATATTGCAGCCAAGATTAATGCTCTCAATCTCTCGAAGAATGAGAACACTGCTGACAATTACCAGTTGCAACTACAGTACCATGAAGTTCATGGCAGTGTTGATGATGGAGCAATATTCAGTTTTCATGTTTCTGGTCGATTTACTTCACTAGTTCCAATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

292

Amino Acids

32.76

Weight (kDa)

6.25

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 93 - 162 2e-08 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 510
Acc16I TGCGCA 1 cut(s) 13
AccB1I GGYRCC 2 cut(s) 496, 565
AccI GTMKAC 1 cut(s) 408
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 58, 342, 482
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 2 cut(s) 18, 318
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 2 cut(s) 239, 788
AfiI CCNNNNNNNGG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 4 cut(s) 262, 330, 457, 667
AhlI ACTAGT 2 cut(s) 545, 859
AjnI CCWGG 1 cut(s) 430
AluBI AGCT 3 cut(s) 265, 296, 358
AluI AGCT 3 cut(s) 265, 296, 358
AlwI GGATC 1 cut(s) 206
ApeKI GCWGC 2 cut(s) 116, 716
AseI ATTAAT 1 cut(s) 726
AspLEI GCGC 2 cut(s) 14, 32
AspS9I GGNCC 1 cut(s) 604
AsuHPI GGTGA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 604
AxyI CCTNAGG 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 770, 803
BanI GGYRCC 2 cut(s) 496, 565
BbvI GCAGC 2 cut(s) 103, 728
BccI CCATC 1 cut(s) 809
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 1 cut(s) 432
BciVI GTATCC 1 cut(s) 96
BclI TGATCA 1 cut(s) 559
BcuI ACTAGT 2 cut(s) 545, 859
BfaI CTAG 3 cut(s) 293, 546, 860
BfmI CTRYAG 1 cut(s) 782
BfuI GTATCC 1 cut(s) 96
BglII AGATCT 1 cut(s) 165
BisI GCNGC 2 cut(s) 117, 717
BlsI GCNGC 2 cut(s) 118, 718
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 2 cut(s) 498, 567
BmrFI CCNGG 1 cut(s) 432
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 655
BpmI CTGGAG 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 431
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 531, 661, 772
Bse21I CCTNAGG 1 cut(s) 194
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 3 cut(s) 267, 601, 652
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMII CTCAG 2 cut(s) 208, 504
BseNI ACTGG 3 cut(s) 531, 661, 772
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 2 cut(s) 103, 728
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 280
BshNI GGYRCC 2 cut(s) 496, 565
BsiEI CGRYCG 1 cut(s) 280
BsiWI CGTACG 1 cut(s) 237
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 386
Bsp143I GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
BspACI CCGC 3 cut(s) 58, 342, 482
BspCNI CTCAG 2 cut(s) 207, 503
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 498, 567
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 496, 565
BsrI ACTGG 3 cut(s) 531, 661, 772
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
BssNI GRCGYC 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 3 cut(s) 137, 426, 786
BstACI GRCGYC 1 cut(s) 507
BstC8I GCNNGC 4 cut(s) 90, 94, 386, 482
BstDEI CTNAG 4 cut(s) 194, 354, 490, 699
BstF5I GGATG 3 cut(s) 267, 601, 652
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 2 cut(s) 14, 32
BstKTI GATC 7 cut(s) 168, 174, 201, 280, 369, 562, 578
BstMBI GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 20, 29, 36, 159
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 2 cut(s) 342, 623
BstSCI CCNGG 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 782
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 103, 728
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
Bsu36I CCTNAGG 1 cut(s) 194
BsuI GTATCC 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 454
BtsCI GGATG 3 cut(s) 267, 601, 652
BtsI GCAGTG 4 cut(s) 111, 411, 756, 811
BtsIMutI CAGTG 4 cut(s) 111, 411, 756, 811
Cac8I GCNNGC 4 cut(s) 90, 94, 386, 482
CfoI GCGC 2 cut(s) 14, 32
Cfr13I GGNCC 1 cut(s) 604
Csp6I GTAC 2 cut(s) 238, 787
CviAII CATG 7 cut(s) 309, 339, 521, 620, 791, 800, 836
CviJI RGCY 9 cut(s) 23, 67, 88, 185, 265, 296, 358, 682, 719
CviKI_1 RGCY 9 cut(s) 23, 67, 88, 185, 265, 296, 358, 682, 719
CviQI GTAC 2 cut(s) 238, 787
DdeI CTNAG 4 cut(s) 194, 354, 490, 699
DpnI GATC 7 cut(s) 167, 173, 200, 279, 368, 561, 577
DpnII GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
Eco47I GGWCC 1 cut(s) 604
Eco57I CTGAAG 2 cut(s) 18, 318
Eco81I CCTNAGG 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 430
FaeI CATG 7 cut(s) 312, 342, 524, 623, 794, 803, 839
FaqI GGGAC 1 cut(s) 386
FatI CATG 7 cut(s) 308, 338, 520, 619, 790, 799, 835
FauI CCCGC 2 cut(s) 335, 489
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 117, 717
FokI GGATG 3 cut(s) 254, 608, 659
Fsp4HI GCNGC 2 cut(s) 117, 717
FspBI CTAG 3 cut(s) 293, 546, 860
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 2 cut(s) 13, 31
GluI GCNGC 2 cut(s) 117, 717
GsuI CTGGAG 1 cut(s) 103
HhaI GCGC 2 cut(s) 14, 32
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 7 cut(s) 312, 342, 524, 623, 794, 803, 839
Hin6I GCGC 2 cut(s) 12, 30
HinP1I GCGC 2 cut(s) 12, 30
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 7 cut(s) 71, 76, 127, 227, 349, 436, 488
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 4 cut(s) 409, 445, 625, 694
Hpy188I TCNGA 3 cut(s) 221, 493, 583
Hpy188III TCNNGA 7 cut(s) 124, 169, 196, 330, 557, 676, 742
Hpy8I GTNNAC 4 cut(s) 409, 445, 625, 694
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 3 cut(s) 137, 426, 786
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 3 cut(s) 153, 716, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 29, 36, 159
HpyF3I CTNAG 4 cut(s) 194, 354, 490, 699
HpySE526I ACGT 1 cut(s) 507
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 7 cut(s) 312, 342, 524, 623, 794, 803, 839
HspAI GCGC 2 cut(s) 12, 30
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
LmnI GCTCC 1 cut(s) 818
Lsp1109I GCAGC 2 cut(s) 103, 728
LweI GCATC 1 cut(s) 23
MaeI CTAG 3 cut(s) 293, 546, 860
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 2 cut(s) 155, 389
MalI GATC 7 cut(s) 167, 173, 200, 279, 368, 561, 577
MboI GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
MboII GAAGA 5 cut(s) 42, 250, 318, 543, 757
MflI RGATCY 1 cut(s) 165
MluCI AATT 3 cut(s) 632, 766, 867
MlyI GAGTC 2 cut(s) 70, 482
MnlI CCTC 8 cut(s) 12, 78, 175, 203, 281, 388, 487, 547
MseI TTAA 3 cut(s) 335, 420, 726
MslI CAYNNNNRTG 1 cut(s) 804
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 4 cut(s) 20, 29, 36, 159
NdeII GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
NlaIII CATG 7 cut(s) 312, 342, 524, 623, 794, 803, 839
NlaIV GGNNCC 2 cut(s) 498, 567
NsbI TGCGCA 1 cut(s) 13
NspI RCATGY 2 cut(s) 342, 623
PciI ACATGT 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 5 cut(s) 71, 127, 227, 349, 436
Pfl23II CGTACG 1 cut(s) 237
PflFI GACNNNGTC 1 cut(s) 602
PkrI GCNGC 2 cut(s) 118, 718
Ple19I CGATCG 1 cut(s) 280
PleI GAGTC 2 cut(s) 70, 482
PpsI GAGTC 2 cut(s) 70, 482
PscI ACATGT 1 cut(s) 619
PshBI ATTAAT 1 cut(s) 726
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 498, 567
PspPI GGNCC 1 cut(s) 604
PsuI RGATCY 1 cut(s) 165
PsyI GACNNNGTC 1 cut(s) 602
PvuI CGATCG 1 cut(s) 280
RsaI GTAC 2 cut(s) 239, 788
RsaNI GTAC 2 cut(s) 238, 787
RseI CAYNNNNRTG 1 cut(s) 804
SalI GTCGAC 1 cut(s) 407
SaqAI TTAA 3 cut(s) 335, 420, 726
SatI GCNGC 2 cut(s) 117, 717
Sau3AI GATC 7 cut(s) 165, 171, 198, 277, 366, 559, 575
Sau96I GGNCC 1 cut(s) 604
SchI GAGTC 2 cut(s) 70, 482
ScrFI CCNGG 1 cut(s) 432
SetI ASST 9 cut(s) 195, 267, 294, 298, 360, 455, 498, 510, 606
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 1 cut(s) 782
SinI GGWCC 1 cut(s) 604
SmiMI CAYNNNNRTG 1 cut(s) 804
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SpeI ACTAGT 2 cut(s) 545, 859
Sse9I AATT 3 cut(s) 632, 766, 867
SsiI CCGC 3 cut(s) 58, 342, 482
SspI AATATT 1 cut(s) 825
SspMI CTAG 3 cut(s) 293, 546, 860
StyD4I CCNGG 1 cut(s) 430
TaaI ACNGT 3 cut(s) 137, 426, 786
TaiI ACGT 1 cut(s) 510
TaqI TCGA 8 cut(s) 17, 74, 174, 201, 249, 408, 743, 847
TasI AATT 3 cut(s) 632, 766, 867
TfiI GAWTC 5 cut(s) 71, 127, 227, 349, 436
Tru1I TTAA 3 cut(s) 335, 420, 726
Tru9I TTAA 3 cut(s) 335, 420, 726
TscAI CASTG 4 cut(s) 118, 418, 763, 811
TseI GCWGC 2 cut(s) 116, 716
TspDTI ATGAA 7 cut(s) 119, 240, 297, 537, 788, 807, 824
TspRI CASTG 4 cut(s) 118, 418, 763, 811
Tth111I GACNNNGTC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 604
VspI ATTAAT 1 cut(s) 726
XceI RCATGY 2 cut(s) 342, 623
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 3 cut(s) 293, 546, 860
ZraI GACGTC 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.