Rh3DG291500

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0010577
Physical Location & Seq
Reverse (-)
30120736 .. 30121773
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG291500.1

Sequence Viewer

Length: 951 bp
ATGTGGGGGTGGGGGTGGGGAAAAGCTTCAAGGTGCAAGAAACTACTCAAACAAGTCCGGTCCCGGCTTGAGTCCTTGAAAGAGGAAAGGTCTACTATTTCTACAGAGTTAGGGAAAGATGTTGAGGAGCTAGACTACAAGGCACTGAACCTCAGGGTCGAACAGGAACAGCATATCAGAGACGAATCTTCGGTGTACACTTTGCTCGACCATTTCTGCAAGCTCATCCTCATGCACTTCTTTTATATACGAACGCACAGGCAATGTCGTCAAAGAGACCTAGCTGTAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGCTTAGGACGTGGGGTGATTCCTGAGCTTGAGGAGATCAGGGACTTACTCGCAGAGCGTTATGGGGAGAGTTTTGTTGAAACTGCTGTGAAGTTGTTGCCTGGGAATCTTGTAAACCCTAACTTCCAGAAGAATATTCTCGCTCTCTCCCCAGAAACAGTGGTGCCTAATGATATAGTAAAACATGAACTGGTTAAACCCTCTTTTACTACTTCTTCTCCTGATGAGGCACCAGCAAGATCATCAGCAGATAAAAAGGACGACGAGAATCGGAACAATGAATTACAACTTATCAGCTTACAATCTCCTATGCATGTCCACCCCAAATTGCCTGATTGGGATGATATATTTACTAAGTTCAGTGCTCTCAAGAAGCCTAAGTATTTGCACCCTAAGTTGCCGGAATATGATGATATTACAGCCAAGTTTAATGCTCTCAAGATCCCCATGAATGAGAACCCTCCTAATAACTACCAGTTGCAATTAGAGTACCATGACGATCATGACAATATTGATGATAGAGCCATATTCGACTATTCCAGGCTCTCGATTGAGAGCAGCTGCAATTTGATAAGCCCGTCCCCAATGTTGTTATTAACGCAATCACCCCATATTGAATACCTAGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

316

Amino Acids

36.43

Weight (kDa)

5.8

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 13 - 158 1.4e-19 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 481, 547
AccI GTMKAC 1 cut(s) 92
AclWI GGATC 1 cut(s) 754
AfaI GTAC 2 cut(s) 197, 809
AgsI TTSAA 6 cut(s) 30, 79, 297, 318, 398, 935
AjiI CACGTC 1 cut(s) 329
AjnI CCWGG 2 cut(s) 418, 857
AluBI AGCT 9 cut(s) 26, 130, 223, 284, 321, 346, 615, 879, 944
AluI AGCT 9 cut(s) 26, 130, 223, 284, 321, 346, 615, 879, 944
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 2 cut(s) 174, 270
AlwI GGATC 1 cut(s) 754
ApeKI GCWGC 2 cut(s) 876, 879
Asp700I GAANNNNTTC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 60
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 2 cut(s) 346, 915
AvaII GGWCC 1 cut(s) 60
AxyI CCTNAGG 1 cut(s) 152
BanI GGYRCC 2 cut(s) 481, 547
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 2 cut(s) 866, 888
BciT130I CCWGG 2 cut(s) 420, 859
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 174, 270
BfaI CTAG 3 cut(s) 131, 281, 941
BfmI CTRYAG 2 cut(s) 102, 285
BisI GCNGC 2 cut(s) 877, 880
BlsI GCNGC 2 cut(s) 878, 881
Bme1390I CCNGG 3 cut(s) 64, 420, 859
Bme18I GGWCC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 3 cut(s) 62, 483, 549
BmrFI CCNGG 3 cut(s) 64, 420, 859
Bpu10I CCTNAGC 2 cut(s) 322, 342
BpuEI CTTGAG 4 cut(s) 89, 368, 671, 740
BpuMI CCSGG 1 cut(s) 64
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bse1I ACTGG 2 cut(s) 513, 793
Bse21I CCTNAGG 1 cut(s) 152
Bse3DI GCAATG 1 cut(s) 269
BseBI CCWGG 2 cut(s) 420, 859
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 2 cut(s) 225, 664
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 166, 333
BseNI ACTGG 2 cut(s) 513, 793
BseRI GAGGAG 2 cut(s) 140, 365
BseXI GCAGC 2 cut(s) 866, 888
BshNI GGYRCC 2 cut(s) 481, 547
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 3 cut(s) 58, 64, 719
BslFI GGGAC 3 cut(s) 46, 374, 883
BsmAI GTCTC 2 cut(s) 174, 270
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 3 cut(s) 46, 374, 883
Bso31I GGTCTC 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 685
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 4 cut(s) 354, 557, 759, 817
BspCNI CTCAG 2 cut(s) 165, 334
BspHI TCATGA 1 cut(s) 820
BspLI GGNNCC 3 cut(s) 62, 483, 549
BspPI GGATC 1 cut(s) 754
BspT107I GGYRCC 2 cut(s) 481, 547
BspTNI GGTCTC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 2 cut(s) 513, 793
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 4 cut(s) 354, 557, 759, 817
Bst2UI CCWGG 2 cut(s) 420, 859
Bst4CI ACNGT 1 cut(s) 478
BstAUI TGTACA 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 6 cut(s) 152, 322, 342, 672, 696, 711
BstF5I GGATG 2 cut(s) 225, 664
BstKTI GATC 4 cut(s) 357, 560, 762, 820
BstMAI GTCTC 2 cut(s) 174, 270
BstMBI GATC 4 cut(s) 354, 557, 759, 817
BstNI CCWGG 2 cut(s) 420, 859
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 3 cut(s) 62, 418, 857
BstSFI CTRYAG 2 cut(s) 102, 285
BstV1I GCAGC 2 cut(s) 866, 888
BstX2I RGATCY 1 cut(s) 759
BstYI RGATCY 1 cut(s) 759
Bsu36I CCTNAGG 1 cut(s) 152
BtrI CACGTC 1 cut(s) 329
BtsCI GGATG 2 cut(s) 225, 664
BtsIMutI CAGTG 3 cut(s) 143, 483, 685
Cac8I GCNNGC 1 cut(s) 221
CciI TCATGA 1 cut(s) 820
Cfr13I GGNCC 1 cut(s) 60
Csp6I GTAC 2 cut(s) 196, 808
CviAII CATG 6 cut(s) 232, 503, 632, 766, 812, 821
CviQI GTAC 2 cut(s) 196, 808
DdeI CTNAG 6 cut(s) 152, 322, 342, 672, 696, 711
DpnI GATC 4 cut(s) 356, 559, 761, 819
DpnII GATC 4 cut(s) 354, 557, 759, 817
Eco31I GGTCTC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 60
Eco81I CCTNAGG 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 418, 857
EcoT22I ATGCAT 1 cut(s) 633
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 6 cut(s) 235, 506, 635, 769, 815, 824
FaqI GGGAC 3 cut(s) 46, 374, 883
FatI CATG 6 cut(s) 231, 502, 631, 765, 811, 820
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 2 cut(s) 877, 880
FokI GGATG 2 cut(s) 212, 671
Fsp4HI GCNGC 2 cut(s) 877, 880
FspBI CTAG 3 cut(s) 131, 281, 941
GluI GCNGC 2 cut(s) 877, 880
HapII CCGG 3 cut(s) 58, 64, 719
Hin1II CATG 6 cut(s) 235, 506, 635, 769, 815, 824
HindIII AAGCTT 2 cut(s) 24, 319
HinfI GANTC 5 cut(s) 71, 185, 337, 424, 586
HpaII CCGG 3 cut(s) 58, 64, 719
HphI GGTGA 2 cut(s) 346, 915
Hpy166II GTNNAC 5 cut(s) 93, 196, 198, 433, 637
Hpy188I TCNGA 2 cut(s) 179, 591
Hpy188III TCNNGA 7 cut(s) 341, 445, 539, 688, 757, 821, 865
Hpy8I GTNNAC 5 cut(s) 93, 196, 198, 433, 637
Hpy99I CGWCG 1 cut(s) 584
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 7 cut(s) 36, 219, 235, 631, 706, 799, 882
HpyF3I CTNAG 6 cut(s) 152, 322, 342, 672, 696, 711
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 6 cut(s) 235, 506, 635, 769, 815, 824
Kzo9I GATC 4 cut(s) 354, 557, 759, 817
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 2 cut(s) 866, 888
MaeI CTAG 3 cut(s) 131, 281, 941
MaeII ACGT 1 cut(s) 328
MalI GATC 4 cut(s) 356, 559, 761, 819
MboI GATC 4 cut(s) 354, 557, 759, 817
MboII GAAGA 4 cut(s) 180, 306, 460, 525
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 4 cut(s) 599, 644, 800, 883
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 8 cut(s) 76, 118, 161, 239, 343, 529, 538, 789
Mph1103I ATGCAT 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 3 cut(s) 513, 747, 914
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 879
MspI CCGG 3 cut(s) 58, 64, 719
MspR9I CCNGG 3 cut(s) 64, 420, 859
MvaI CCWGG 2 cut(s) 420, 859
NciI CCSGG 1 cut(s) 64
NdeII GATC 4 cut(s) 354, 557, 759, 817
NlaIII CATG 6 cut(s) 235, 506, 635, 769, 815, 824
NlaIV GGNNCC 3 cut(s) 62, 483, 549
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 635
PagI TCATGA 1 cut(s) 820
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 185, 337, 424, 586
PkrI GCNGC 2 cut(s) 878, 881
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 418, 857
PspGI CCWGG 2 cut(s) 418, 857
PspN4I GGNNCC 3 cut(s) 62, 483, 549
PspPI GGNCC 1 cut(s) 60
PsuI RGATCY 1 cut(s) 759
PvuII CAGCTG 1 cut(s) 879
RsaI GTAC 2 cut(s) 197, 809
RsaNI GTAC 2 cut(s) 196, 808
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 3 cut(s) 513, 747, 914
SatI GCNGC 2 cut(s) 877, 880
Sau3AI GATC 4 cut(s) 354, 557, 759, 817
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 3 cut(s) 64, 420, 859
SduI GDGCHC 1 cut(s) 685
SfcI CTRYAG 2 cut(s) 102, 285
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 4 cut(s) 68, 347, 686, 755
SmoI CTYRAG 4 cut(s) 68, 347, 686, 755
Sse9I AATT 4 cut(s) 599, 644, 800, 883
SspI AATATT 2 cut(s) 454, 829
SspMI CTAG 3 cut(s) 131, 281, 941
StyD4I CCNGG 3 cut(s) 62, 418, 857
TaaI ACNGT 1 cut(s) 478
TaiI ACGT 1 cut(s) 331
TaqI TCGA 4 cut(s) 159, 207, 849, 866
TasI AATT 4 cut(s) 599, 644, 800, 883
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 4 cut(s) 185, 337, 424, 586
Tru1I TTAA 3 cut(s) 513, 747, 914
Tru9I TTAA 3 cut(s) 513, 747, 914
TscAI CASTG 3 cut(s) 150, 483, 685
TseI GCWGC 2 cut(s) 876, 879
TspDTI ATGAA 3 cut(s) 519, 612, 782
TspRI CASTG 3 cut(s) 150, 483, 685
VpaK11BI GGWCC 1 cut(s) 60
XceI RCATGY 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 3 cut(s) 131, 281, 941
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.