Rh3BG298200

Regulator of Vps4 activity in the MVB pathway protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010577
Physical Location & Seq
Reverse (-)
29924755 .. 29925749
995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG298200.1

Sequence Viewer

Length: 720 bp
ATGCACTTCTTTTATATACGAACGCACAGGCAATGTCGTCAAAGAGACCTAGCTGTAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGCTTAGGACGTGGGGTGATTCCTGAGCTTGAGGAGATCAGGGACTTACTCGGAGAGCGTTACGGGGAGAGTTTTGTTGAAACTGCTGTGAAGTTGTTGCCTGGGAATCTTGTAAACCCTAACTTCCAGAAGAATATTCTCGCTCTCTCCCCAGAAACAGTGGTGCCTAATGATATAGTAAAACATGAACTGGTTAAACCCTCTTTTACTACTTCTTCTCCTGATGAGGCACCAGAAAGATCATCAGCAGAGAAAAAGGATGACGAGAATCGGAACAATGAATTACAACTTATCAGCTTACAATCTCCTATGCATGTCCACCCCAAATTGCCTGATTGGGATGATATATTTACTAAGTTCAGTGCTCTCAAGAAGCCTAAGTATTTGCACCCTAAGTTGCCGGAATATGATGATATTACAGCCAAGTTTAATGCTCTCAAGATCCCCATGAATGAGAACCCTCCTAATAACTACCAGTTGCAATTAGAGTACCATGATGATCATGACAATATTGATGATAGAGCCATATTCGACTATTCCAGGCTCTCGATTGAGAGCAGCTGCAATTTGATAAGCCCGTCCCCAATGTTGTTATTAACGCAATCACCCCATATTGAACACCTAGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

239

Amino Acids

27.36

Weight (kDa)

5.45

Isoelectric Point (pI)

60.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 3 - 82 5.3e-11 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 250, 316
AclWI GGATC 1 cut(s) 523
AfaI GTAC 1 cut(s) 578
AgsI TTSAA 4 cut(s) 66, 87, 167, 704
AjiI CACGTC 1 cut(s) 98
AjnI CCWGG 2 cut(s) 187, 626
AluBI AGCT 6 cut(s) 53, 90, 115, 384, 648, 713
AluI AGCT 6 cut(s) 53, 90, 115, 384, 648, 713
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 645, 648
AsuHPI GGTGA 2 cut(s) 115, 684
BanI GGYRCC 2 cut(s) 250, 316
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 2 cut(s) 635, 657
BciT130I CCWGG 2 cut(s) 189, 628
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 2 cut(s) 50, 710
BfmI CTRYAG 1 cut(s) 54
BisI GCNGC 2 cut(s) 646, 649
BlsI GCNGC 2 cut(s) 647, 650
Bme1390I CCNGG 2 cut(s) 189, 628
BmgBI CACGTC 1 cut(s) 98
BmiI GGNNCC 2 cut(s) 252, 318
BmrFI CCNGG 2 cut(s) 189, 628
Bpu10I CCTNAGC 2 cut(s) 91, 111
BpuEI CTTGAG 3 cut(s) 137, 440, 509
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 188
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bse1I ACTGG 2 cut(s) 282, 562
Bse3DI GCAATG 1 cut(s) 38
BseBI CCWGG 2 cut(s) 189, 628
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 2 cut(s) 352, 433
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 2 cut(s) 282, 562
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 2 cut(s) 635, 657
BshNI GGYRCC 2 cut(s) 250, 316
BsiHKAI GWGCWC 1 cut(s) 454
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 2 cut(s) 143, 652
BsmAI GTCTC 1 cut(s) 39
BsmFI GGGAC 2 cut(s) 143, 652
Bso31I GGTCTC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 4 cut(s) 123, 326, 528, 586
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 252, 318
BspPI GGATC 1 cut(s) 523
BspT107I GGYRCC 2 cut(s) 250, 316
BspTNI GGTCTC 1 cut(s) 39
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 2 cut(s) 282, 562
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 4 cut(s) 123, 326, 528, 586
Bst2UI CCWGG 2 cut(s) 189, 628
Bst4CI ACNGT 1 cut(s) 247
BstDEI CTNAG 5 cut(s) 91, 111, 441, 465, 480
BstF5I GGATG 2 cut(s) 352, 433
BstKTI GATC 4 cut(s) 126, 329, 531, 589
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 4 cut(s) 123, 326, 528, 586
BstNI CCWGG 2 cut(s) 189, 628
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 187, 626
BstSFI CTRYAG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 635, 657
BstX2I RGATCY 1 cut(s) 528
BstYI RGATCY 1 cut(s) 528
BtrI CACGTC 1 cut(s) 98
BtsCI GGATG 2 cut(s) 352, 433
BtsIMutI CAGTG 2 cut(s) 252, 454
CciI TCATGA 1 cut(s) 589
Csp6I GTAC 1 cut(s) 577
CviAII CATG 5 cut(s) 272, 401, 535, 581, 590
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 5 cut(s) 91, 111, 441, 465, 480
DpnI GATC 4 cut(s) 125, 328, 530, 588
DpnII GATC 4 cut(s) 123, 326, 528, 586
Eco31I GGTCTC 1 cut(s) 39
EcoRII CCWGG 2 cut(s) 187, 626
EcoT22I ATGCAT 1 cut(s) 402
FaeI CATG 5 cut(s) 275, 404, 538, 584, 593
FaqI GGGAC 2 cut(s) 143, 652
FatI CATG 5 cut(s) 271, 400, 534, 580, 589
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 2 cut(s) 646, 649
FokI GGATG 2 cut(s) 359, 440
Fsp4HI GCNGC 2 cut(s) 646, 649
FspBI CTAG 2 cut(s) 50, 710
GluI GCNGC 2 cut(s) 646, 649
HapII CCGG 1 cut(s) 488
Hin1II CATG 5 cut(s) 275, 404, 538, 584, 593
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 3 cut(s) 106, 193, 355
HpaII CCGG 1 cut(s) 488
HphI GGTGA 2 cut(s) 115, 684
Hpy166II GTNNAC 2 cut(s) 202, 406
Hpy188I TCNGA 2 cut(s) 140, 360
Hpy188III TCNNGA 7 cut(s) 110, 214, 308, 457, 526, 590, 634
Hpy8I GTNNAC 2 cut(s) 202, 406
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 5 cut(s) 4, 400, 475, 568, 651
HpyF3I CTNAG 5 cut(s) 91, 111, 441, 465, 480
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 5 cut(s) 275, 404, 538, 584, 593
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 4 cut(s) 123, 326, 528, 586
Lsp1109I GCAGC 2 cut(s) 635, 657
MaeI CTAG 2 cut(s) 50, 710
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 1 cut(s) 146
MalI GATC 4 cut(s) 125, 328, 530, 588
MboI GATC 4 cut(s) 123, 326, 528, 586
MboII GAAGA 3 cut(s) 75, 229, 294
MflI RGATCY 1 cut(s) 528
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 4 cut(s) 368, 413, 569, 652
MnlI CCTC 4 cut(s) 112, 298, 307, 558
Mph1103I ATGCAT 1 cut(s) 402
MseI TTAA 3 cut(s) 282, 516, 683
MspA1I CMGCKG 1 cut(s) 648
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 2 cut(s) 189, 628
MvaI CCWGG 2 cut(s) 189, 628
NdeII GATC 4 cut(s) 123, 326, 528, 586
NlaIII CATG 5 cut(s) 275, 404, 538, 584, 593
NlaIV GGNNCC 2 cut(s) 252, 318
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 1 cut(s) 404
PagI TCATGA 1 cut(s) 589
PfeI GAWTC 3 cut(s) 106, 193, 355
PkrI GCNGC 2 cut(s) 647, 650
Psp6I CCWGG 2 cut(s) 187, 626
PspGI CCWGG 2 cut(s) 187, 626
PspN4I GGNNCC 2 cut(s) 252, 318
PsuI RGATCY 1 cut(s) 528
PvuII CAGCTG 1 cut(s) 648
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SaqAI TTAA 3 cut(s) 282, 516, 683
SatI GCNGC 2 cut(s) 646, 649
Sau3AI GATC 4 cut(s) 123, 326, 528, 586
ScrFI CCNGG 2 cut(s) 189, 628
SduI GDGCHC 1 cut(s) 454
SetI ASST 9 cut(s) 51, 55, 92, 100, 117, 386, 650, 711, 715
SfcI CTRYAG 1 cut(s) 54
SmlI CTYRAG 3 cut(s) 116, 455, 524
SmoI CTYRAG 3 cut(s) 116, 455, 524
Sse9I AATT 4 cut(s) 368, 413, 569, 652
SspI AATATT 2 cut(s) 223, 598
SspMI CTAG 2 cut(s) 50, 710
StyD4I CCNGG 2 cut(s) 187, 626
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 618, 635
TasI AATT 4 cut(s) 368, 413, 569, 652
TfiI GAWTC 3 cut(s) 106, 193, 355
Tru1I TTAA 3 cut(s) 282, 516, 683
Tru9I TTAA 3 cut(s) 282, 516, 683
TscAI CASTG 2 cut(s) 252, 454
TseI GCWGC 2 cut(s) 645, 648
TspDTI ATGAA 3 cut(s) 288, 381, 551
TspRI CASTG 2 cut(s) 252, 454
XceI RCATGY 1 cut(s) 404
XspI CTAG 2 cut(s) 50, 710
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.