Rroxscaffold_6G00397820

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010577
Physical Location & Seq
Reverse (-)
19530464 .. 19531420
957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397820.1

Sequence Viewer

Length: 957 bp
ATGTTGATGTTGGCCTTATTGTTGGGTTGGAGAAAAGCTTCAAAGTGCAAGAAACTACTCAAACAAGTTCGGTCCCGGCTCGAGTGCTTGAAAAACGAGAGGTCTGCTATTTCTACGCAGTTACGGAAAGAGCTTGAGAAACTAGACGAGGAGGCACTGAACCTCAGGATCGAACAAGAACAACACATCAGAGACGAATCTTCAGTTTTCAATTTGCTCGACCATTTCTGCAACCTCATCCTTAAGCACTCCTTTTATAATTATATTCCAACGCACAAGCAGTGTCGTCAAAACCTAGCTGAAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGATTAAGATGCGGGGTGATTCCAGAGCTTGAGGAGATCGGGAACTTTTTTGGAGAGTGTTACGGGGAGAGTTTTGTAGAAACTACTGTGAAACTGGTGCCTGGGAATCTTGTAAACCTTACCTTCAAAAAGACTATTCTCGCTCTCTTCCCAGACTCAGTGGTGCCCAATGATAGAGTAAAACAAGAACTGGTAAAACCCTCTTTTACTAGTTCTTCTACTGATGAGGCACCAGAAAGATCATCAACAGCAGAGGAAAAGGACGACGAGGATCGCAACAATAAACTACAACTTATTACCTCCCAATCTTCTAAACATGTCCACCCCAAATTGCCTGACTGGGATGATATATTTACCAAGTTCAGTGCCCTGAAGAAGCCTAAGTACGTCAACCCTAAGTTGCCAGAATATGATGATCTTACAGCCAAGTATAATGCTGTCAAGAGCCCTAATAATGAGAACACTGCTGATAATTACCGCTCGCAATTAGAGTACGATGATGAGCATGATAATATTGATGACGGAGCAATGTTCAGTTATTCATGGTTGTCGATAGAGAGCATTTTGGCAATGATAAACCAGCCCCAATGTGCCAGTGTCATTAACACAATCACCCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

318

Amino Acids

36.44

Weight (kDa)

5.63

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 157 2.5e-19 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccB1I GGYRCC 3 cut(s) 430, 496, 562
AccBSI CCGCTC 1 cut(s) 813
AciI CCGC 2 cut(s) 345, 811
AclWI GGATC 2 cut(s) 176, 612
AcuI CTGAAG 3 cut(s) 186, 321, 725
AfaI GTAC 2 cut(s) 719, 827
AflII CTTAAG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 6 cut(s) 42, 91, 211, 312, 333, 460
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 4 cut(s) 38, 133, 299, 361
AluI AGCT 4 cut(s) 38, 133, 299, 361
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 2 cut(s) 176, 612
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 361, 937
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 2 cut(s) 501, 703
BanI GGYRCC 3 cut(s) 430, 496, 562
BanII GRGCYC 1 cut(s) 782
BarI GAAGNNNNNNTAC 2 cut(s) 701, 733
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 186
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 3 cut(s) 143, 296, 543
BfrI CTTAAG 1 cut(s) 242
Bme1390I CCNGG 2 cut(s) 76, 435
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 4 cut(s) 74, 432, 498, 564
BmrFI CCNGG 2 cut(s) 76, 435
BmrI ACTGGG 1 cut(s) 682
BmsI GCATC 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 682
BpuEI CTTGAG 2 cut(s) 155, 383
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 434
Bse1I ACTGG 4 cut(s) 432, 528, 677, 927
Bse21I CCTNAGG 1 cut(s) 164
Bse3DI GCAATG 2 cut(s) 867, 909
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 434
BseGI GGATG 2 cut(s) 237, 682
BseMI GCAATG 2 cut(s) 867, 909
BseMII CTCAG 2 cut(s) 178, 504
BseNI ACTGG 4 cut(s) 432, 528, 677, 927
BseRI GAGGAG 2 cut(s) 164, 380
BseSI GKGCMC 2 cut(s) 501, 703
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 3 cut(s) 430, 496, 562
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 501, 703, 782
Bsp143I GATC 5 cut(s) 168, 369, 572, 604, 748
BspACI CCGC 2 cut(s) 345, 811
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 177, 503
BspLI GGNNCC 4 cut(s) 74, 432, 498, 564
BspPI GGATC 2 cut(s) 176, 612
BspT107I GGYRCC 3 cut(s) 430, 496, 562
BspTI CTTAAG 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 813
BsrDI GCAATG 2 cut(s) 867, 909
BsrI ACTGG 4 cut(s) 432, 528, 677, 927
BssECI CCNNGG 1 cut(s) 434
BssMI GATC 5 cut(s) 168, 369, 572, 604, 748
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 421
Bst6I CTCTTC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 815
BstDEI CTNAG 4 cut(s) 164, 490, 714, 729
BstF5I GGATG 2 cut(s) 237, 682
BstKTI GATC 5 cut(s) 171, 372, 575, 607, 751
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 5 cut(s) 168, 369, 572, 604, 748
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 2 cut(s) 74, 433
BstSLI GKGCMC 2 cut(s) 501, 703
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 1 cut(s) 14
BtsCI GGATG 2 cut(s) 237, 682
BtsI GCAGTG 2 cut(s) 287, 795
BtsIMutI CAGTG 6 cut(s) 155, 287, 498, 703, 795, 934
Cac8I GCNNGC 1 cut(s) 815
Cfr13I GGNCC 1 cut(s) 72
Csp6I GTAC 2 cut(s) 718, 826
CviAII CATG 3 cut(s) 650, 839, 876
CviQI GTAC 2 cut(s) 718, 826
DdeI CTNAG 4 cut(s) 164, 490, 714, 729
DpnI GATC 5 cut(s) 170, 371, 574, 606, 750
DpnII GATC 5 cut(s) 168, 369, 572, 604, 748
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco24I GRGCYC 1 cut(s) 782
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 186, 321, 725
Eco81I CCTNAGG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 782
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 3 cut(s) 653, 842, 879
FaiI YATR 9 cut(s) 258, 264, 651, 683, 744, 765, 840, 877, 952
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 58
FatI CATG 3 cut(s) 649, 838, 875
FauI CCCGC 1 cut(s) 338
FokI GGATG 2 cut(s) 224, 689
FriOI GRGCYC 1 cut(s) 782
FspBI CTAG 3 cut(s) 143, 296, 543
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 76
Hin1II CATG 3 cut(s) 653, 842, 879
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 4 cut(s) 197, 352, 439, 488
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 361, 937
Hpy166II GTNNAC 3 cut(s) 448, 655, 724
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 5 cut(s) 166, 333, 356, 373, 775
Hpy8I GTNNAC 3 cut(s) 448, 655, 724
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 2 cut(s) 48, 231
HpyF3I CTNAG 4 cut(s) 164, 490, 714, 729
HpySE526I ACGT 1 cut(s) 720
Hsp92II CATG 3 cut(s) 653, 842, 879
Kzo9I GATC 5 cut(s) 168, 369, 572, 604, 748
LmnI GCTCC 1 cut(s) 857
LweI GCATC 1 cut(s) 332
MaeI CTAG 3 cut(s) 143, 296, 543
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 2 cut(s) 120, 392
MalI GATC 5 cut(s) 170, 371, 574, 606, 750
MbiI CCGCTC 1 cut(s) 813
MboI GATC 5 cut(s) 168, 369, 572, 604, 748
MboII GAAGA 6 cut(s) 192, 321, 472, 540, 633, 718
MhlI GDGCHC 3 cut(s) 501, 703, 782
MluCI AATT 5 cut(s) 211, 259, 662, 805, 818
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 2 cut(s) 8, 293
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 3 cut(s) 243, 338, 936
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 76, 435
MvaI CCWGG 1 cut(s) 435
NciI CCSGG 1 cut(s) 76
NdeII GATC 5 cut(s) 168, 369, 572, 604, 748
NlaIII CATG 3 cut(s) 653, 842, 879
NlaIV GGNNCC 4 cut(s) 74, 432, 498, 564
NspI RCATGY 1 cut(s) 653
PaeR7I CTCGAG 1 cut(s) 80
PciI ACATGT 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 197, 352, 439
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PscI ACATGT 1 cut(s) 649
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 4 cut(s) 74, 432, 498, 564
PspPI GGNCC 1 cut(s) 72
PspXI VCTCGAGB 1 cut(s) 80
RsaI GTAC 2 cut(s) 719, 827
RsaNI GTAC 2 cut(s) 718, 826
SaqAI TTAA 3 cut(s) 243, 338, 936
Sau3AI GATC 5 cut(s) 168, 369, 572, 604, 748
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 76, 435
SduI GDGCHC 3 cut(s) 501, 703, 782
SfaNI GCATC 1 cut(s) 332
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 4 cut(s) 80, 134, 242, 362
SmoI CTYRAG 4 cut(s) 80, 134, 242, 362
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 5 cut(s) 211, 259, 662, 805, 818
SsiI CCGC 2 cut(s) 345, 811
SspI AATATT 1 cut(s) 847
SspMI CTAG 3 cut(s) 143, 296, 543
StyD4I CCNGG 2 cut(s) 74, 433
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 1 cut(s) 723
TaqI TCGA 4 cut(s) 81, 171, 219, 884
TaqII GACCGA 1 cut(s) 60
TasI AATT 5 cut(s) 211, 259, 662, 805, 818
TfiI GAWTC 3 cut(s) 197, 352, 439
Tru1I TTAA 3 cut(s) 243, 338, 936
Tru9I TTAA 3 cut(s) 243, 338, 936
TscAI CASTG 6 cut(s) 162, 287, 498, 703, 802, 934
TspDTI ATGAA 1 cut(s) 864
TspGWI ACGGA 2 cut(s) 139, 870
TspRI CASTG 6 cut(s) 162, 287, 498, 703, 802, 934
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 72
XceI RCATGY 1 cut(s) 653
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 3 cut(s) 143, 296, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.