Rh3AG259400

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010577
Physical Location & Seq
Forward (+)
28822392 .. 28823753
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG259400.1

Sequence Viewer

Length: 891 bp
ATGTTGATGTTGGCCTTATTGTTGGGTTGGAGAAAAGCTTCAAAGTGCAAGAAACTACTCAAACAAGTTCGGTCCCGGCTCGAGTGCTTGAAAAACGAGAGGTCTGCTATTTCTACGCAGTTACGGAAAGAGCTTGAGAAACTAGACGACGAGGCACTGAACCTCAGGATCGAACAAGAACAACACATCAGAGACGAATCTTCGGTTTACAATTTGCTCGACCATTTCTGCAACCTCATCCTTAAGCACTCCTTTTATAATTATATTCGAACGCACAAGCAGTGTCGTCAAAGAAACCTAGCTGAAGCAGTTTCAAGTCTTGTCTTTGCTTCTTCAAGATTAAGATGCGGGGTGATTCCAGAGCTTGAGGAGATCAGGGACCTTTTTGGAGAGCGTTACGGGGAGAGTTTTGTAGAAACTACTGTGAAACTGGTGCCTGGGAATCGTGTAAACCCTACCTTCAAAAAGTCTATTCTTGCTCTCTTCCCAGACTCAGTGGTGCCCAATGATAGAGTAAAACAAGAACTGGTAAAACCCTCTTTTACTAGTTCTTCTACTGATGAGGCACCAGAAAGATCATCAACAGCAGAGGAAAAGGACGACGAGGATCGCAACAATAAACTACAACTTATTACCTTCCAATCTTCTAAACACGTCCACCCCAAATTGCCTGACTGGGATGATATATTTACCAAGTTCAGAGCCCTCAAGAAGCCTAAGTACGTCAACCCTAAGTTGCCAGAATATGATGATATTACAGCCAAGGATAAGGCTGTCAAGAGCCCTATTATTAATAATGAGAACACTACTGCTGATAATTACCGCTCGCAATTAGAGTACGATGATGAGCATGATAATATTGATGACGGAGCAATTCTCACCTTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

296

Amino Acids

34.27

Weight (kDa)

6.68

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 158 6.1e-22 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccB1I GGYRCC 3 cut(s) 433, 499, 565
AccBSI CCGCTC 1 cut(s) 825
AciI CCGC 2 cut(s) 348, 823
AclWI GGATC 2 cut(s) 176, 615
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 2 cut(s) 722, 839
AflII CTTAAG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 5 cut(s) 42, 91, 315, 336, 463
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 655
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 5 cut(s) 38, 133, 302, 364, 888
AluI AGCT 5 cut(s) 38, 133, 302, 364, 888
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 2 cut(s) 176, 615
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 1 cut(s) 12
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 72, 379
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 364, 871
AsuII TTCGAA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 2 cut(s) 72, 379
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 3 cut(s) 433, 499, 565
BanII GRGCYC 2 cut(s) 706, 785
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 186
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 4 cut(s) 143, 299, 546, 889
BfrI CTTAAG 1 cut(s) 242
Bme1390I CCNGG 2 cut(s) 76, 438
Bme18I GGWCC 2 cut(s) 72, 379
BmeT110I CYCGRG 1 cut(s) 80
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 72, 379
BmiI GGNNCC 5 cut(s) 74, 380, 435, 501, 567
BmrFI CCNGG 2 cut(s) 76, 438
BmrI ACTGGG 1 cut(s) 685
BmsI GCATC 1 cut(s) 335
BmuI ACTGGG 1 cut(s) 685
BplI GAGNNNNNCTC 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 268
BpuEI CTTGAG 3 cut(s) 155, 386, 692
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 437, 762
Bse1I ACTGG 3 cut(s) 435, 531, 680
Bse21I CCTNAGG 1 cut(s) 164
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 437, 762
BseGI GGATG 2 cut(s) 237, 685
BseMII CTCAG 2 cut(s) 178, 507
BseNI ACTGG 3 cut(s) 435, 531, 680
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 504
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 3 cut(s) 433, 499, 565
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 2 cut(s) 58, 392
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 58, 392
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 504, 706, 785
Bsp143I GATC 4 cut(s) 168, 372, 575, 607
BspACI CCGC 2 cut(s) 348, 823
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 177, 506
BspLI GGNNCC 5 cut(s) 74, 380, 435, 501, 567
BspPI GGATC 2 cut(s) 176, 615
BspT104I TTCGAA 1 cut(s) 268
BspT107I GGYRCC 3 cut(s) 433, 499, 565
BspTI CTTAAG 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 825
BsrI ACTGG 3 cut(s) 435, 531, 680
BssECI CCNNGG 2 cut(s) 437, 762
BssMI GATC 4 cut(s) 168, 372, 575, 607
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 1 cut(s) 488
BstAFI CTTAAG 1 cut(s) 242
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 1 cut(s) 827
BstDEI CTNAG 4 cut(s) 164, 493, 717, 732
BstF5I GGATG 2 cut(s) 237, 685
BstKTI GATC 4 cut(s) 171, 375, 578, 610
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 4 cut(s) 168, 372, 575, 607
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 74, 436
BstSLI GKGCMC 1 cut(s) 504
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 1 cut(s) 14
BtrI CACGTC 1 cut(s) 655
BtsCI GGATG 2 cut(s) 237, 685
BtsI GCAGTG 1 cut(s) 287
BtsIMutI CAGTG 3 cut(s) 155, 287, 501
Cac8I GCNNGC 1 cut(s) 827
Cfr13I GGNCC 2 cut(s) 72, 379
Csp6I GTAC 2 cut(s) 721, 838
CviAII CATG 1 cut(s) 851
CviQI GTAC 2 cut(s) 721, 838
DdeI CTNAG 4 cut(s) 164, 493, 717, 732
DpnI GATC 4 cut(s) 170, 374, 577, 609
DpnII GATC 4 cut(s) 168, 372, 575, 607
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 2 cut(s) 706, 785
Eco47I GGWCC 2 cut(s) 72, 379
Eco57I CTGAAG 1 cut(s) 324
Eco81I CCTNAGG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 2 cut(s) 706, 785
ErhI CCWWGG 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 1 cut(s) 854
FaiI YATR 5 cut(s) 258, 264, 686, 747, 852
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 2 cut(s) 58, 392
FatI CATG 1 cut(s) 850
FauI CCCGC 1 cut(s) 341
FokI GGATG 2 cut(s) 224, 692
FriOI GRGCYC 2 cut(s) 706, 785
FspBI CTAG 4 cut(s) 143, 299, 546, 889
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 76
Hin1II CATG 1 cut(s) 854
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 36
HinfI GANTC 4 cut(s) 197, 355, 442, 491
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 364, 871
Hpy166II GTNNAC 4 cut(s) 208, 451, 658, 727
Hpy188I TCNGA 2 cut(s) 191, 701
Hpy188III TCNNGA 5 cut(s) 166, 336, 359, 709, 778
Hpy8I GTNNAC 4 cut(s) 208, 451, 658, 727
Hpy99I CGWCG 2 cut(s) 152, 605
HpyAV CCTTC 2 cut(s) 469, 646
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 2 cut(s) 654, 723
HpyCH4V TGCA 2 cut(s) 48, 231
HpyF3I CTNAG 4 cut(s) 164, 493, 717, 732
HpySE526I ACGT 2 cut(s) 654, 723
Hsp92II CATG 1 cut(s) 854
Kzo9I GATC 4 cut(s) 168, 372, 575, 607
LmnI GCTCC 1 cut(s) 869
LweI GCATC 1 cut(s) 335
MaeI CTAG 4 cut(s) 143, 299, 546, 889
MaeII ACGT 2 cut(s) 654, 723
MaeIII GTNAC 2 cut(s) 120, 395
MalI GATC 4 cut(s) 170, 374, 577, 609
MbiI CCGCTC 1 cut(s) 825
MboI GATC 4 cut(s) 168, 372, 575, 607
MboII GAAGA 5 cut(s) 192, 324, 475, 543, 636
MhlI GDGCHC 3 cut(s) 504, 706, 785
MluCI AATT 6 cut(s) 211, 259, 665, 817, 830, 873
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 8
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 3 cut(s) 243, 341, 792
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 76, 438
MvaI CCWGG 1 cut(s) 438
NciI CCSGG 1 cut(s) 76
NdeII GATC 4 cut(s) 168, 372, 575, 607
NlaIII CATG 1 cut(s) 854
NlaIV GGNNCC 5 cut(s) 74, 380, 435, 501, 567
NspV TTCGAA 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 197, 355, 442
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 792
PsiI TTATAA 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 5 cut(s) 74, 380, 435, 501, 567
PspPI GGNCC 2 cut(s) 72, 379
PspPPI RGGWCCY 1 cut(s) 379
PspXI VCTCGAGB 1 cut(s) 80
RsaI GTAC 2 cut(s) 722, 839
RsaNI GTAC 2 cut(s) 721, 838
SaqAI TTAA 3 cut(s) 243, 341, 792
Sau3AI GATC 4 cut(s) 168, 372, 575, 607
Sau96I GGNCC 2 cut(s) 72, 379
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 76, 438
SduI GDGCHC 3 cut(s) 504, 706, 785
SfaNI GCATC 1 cut(s) 335
Sfr274I CTCGAG 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 2 cut(s) 72, 379
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 5 cut(s) 80, 134, 242, 365, 707
SmoI CTYRAG 5 cut(s) 80, 134, 242, 365, 707
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 6 cut(s) 211, 259, 665, 817, 830, 873
SsiI CCGC 2 cut(s) 348, 823
SspI AATATT 1 cut(s) 859
SspMI CTAG 4 cut(s) 143, 299, 546, 889
StyD4I CCNGG 2 cut(s) 74, 436
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 2 cut(s) 657, 726
TaqI TCGA 4 cut(s) 81, 171, 219, 268
TaqII GACCGA 1 cut(s) 60
TasI AATT 6 cut(s) 211, 259, 665, 817, 830, 873
TfiI GAWTC 3 cut(s) 197, 355, 442
Tru1I TTAA 3 cut(s) 243, 341, 792
Tru9I TTAA 3 cut(s) 243, 341, 792
TscAI CASTG 3 cut(s) 162, 287, 501
TspGWI ACGGA 2 cut(s) 139, 882
TspRI CASTG 3 cut(s) 162, 287, 501
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 72, 379
VspI ATTAAT 1 cut(s) 792
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 4 cut(s) 143, 299, 546, 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.