FvH4_6g24690

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010577
Physical Location & Seq
Forward (+)
18717419 .. 18718464
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24690.t1

Sequence Viewer

Length: 885 bp
ATGTTGATGTTGGGTTGGGCGAAGGCTTCAAAGTGCAAGAAACTACTCAAACAAGTACGGTCTCGGCTCGAGTGCTTGGAACAAGAGAGGTTTGCTACTTCTATGCAGTTACGGCAAGATCTTGATCAGAAGACAGACTACGAGGCTCTCAACATCAGGATCGAACAAGAACAACACATCAGAGACGAATCATCGGCGTACGATTTGCTCGACTATTTCTGCAAGCTCATCCTCAAACACTTGTTTTATATACGAACACACAAGCAGTGTTGTCAAAGAAACCTAGCTGAAGCAGTTTCATGTCTTGTCTTTGCTTCTTCAAGATTAACATGCGGGGTGATTCCTGAGCTTGGGGAGATCAGGGACTTGTTCGGCGAGCGTTACGGGGAGAGTTTTGTCGACACTGCTGTTAAACTGTTGCCTGGGAATCTTGTAAACCCTATCTTCAAAAAGAATATCATCGCTCTCTCCCCGCCAGACTCAGCGGTGCCTACTGACGTCATAGTAAAACATAAACCAGTCGAACCCTCTTTTACTAGTTCTTCTCCTGATCAGGCACCAGCGAGATCATCAGAACAGAAAAAGGATGATGAGGTCCAATGCCCTAAACATGTCCACCCCAAATTGCCTGATTGGGATGATATATATACCCAGTTCAGTGCTCTCAAGAAGCCTAAATATGTGAGCCCTAAGTTGCCCGATATTGCAGCCAAGATTAATGCTCTCAAGATCCCCAAGAATGAGAACACTGCTGATAATTACCAGTTGCAACTACAGTACCATGAAGATCATGGCAGTGTTGATGATGGAGCAATATTCAATTTTCATGGCTGTCGAAACTCGATAGAAAGAATTGAATTTGGAATCCAGCAGTACTGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

295

Amino Acids

33.66

Weight (kDa)

6.75

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 13 - 162 4.1e-23 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 501
AccB1I GGYRCC 2 cut(s) 487, 556
AccI GTMKAC 1 cut(s) 399
AciI CCGC 3 cut(s) 333, 473, 485
AclWI GGATC 2 cut(s) 167, 724
AcsI RAATTY 1 cut(s) 857
AcuI CTGAAG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 498
AfaI GTAC 4 cut(s) 57, 200, 779, 875
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 610
AgsI TTSAA 5 cut(s) 30, 321, 448, 820, 857
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 3 cut(s) 226, 287, 349
AluI AGCT 3 cut(s) 226, 287, 349
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 2 cut(s) 66, 177
AlwI GGATC 2 cut(s) 167, 724
Ama87I CYCGRG 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 1 cut(s) 857
AseI ATTAAT 1 cut(s) 717
AspS9I GGNCC 1 cut(s) 595
AsuHPI GGTGA 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 1 cut(s) 595
BaeI ACNNNNGTAYC 2 cut(s) 761, 794
BanI GGYRCC 2 cut(s) 487, 556
BanII GRGCYC 1 cut(s) 689
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 1 cut(s) 719
BccI CCATC 1 cut(s) 800
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 423
BclI TGATCA 2 cut(s) 124, 550
BcoDI GTCTC 2 cut(s) 66, 177
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 2 cut(s) 284, 537
BfmI CTRYAG 1 cut(s) 773
BglII AGATCT 1 cut(s) 118
BisI GCNGC 1 cut(s) 708
BlsI GCNGC 1 cut(s) 709
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 595
BmeT110I CYCGRG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 2 cut(s) 489, 558
BmrFI CCNGG 1 cut(s) 423
BmrI ACTGGG 1 cut(s) 646
BmuI ACTGGG 1 cut(s) 646
BpiI GAAGAC 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 650, 710
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 422
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse1I ACTGG 3 cut(s) 518, 652, 763
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 422
BseGI GGATG 3 cut(s) 228, 592, 643
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMII CTCAG 2 cut(s) 336, 495
BseNI ACTGG 3 cut(s) 518, 652, 763
BseXI GCAGC 1 cut(s) 719
BshNI GGYRCC 2 cut(s) 487, 556
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 68
BsiWI CGTACG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 2 cut(s) 66, 177
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 377
Bso31I GGTCTC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 68
Bsp1286I GDGCHC 2 cut(s) 664, 689
Bsp143I GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
BspACI CCGC 3 cut(s) 333, 473, 485
BspCNI CTCAG 2 cut(s) 337, 494
BspLI GGNNCC 2 cut(s) 489, 558
BspPI GGATC 2 cut(s) 167, 724
BspT107I GGYRCC 2 cut(s) 487, 556
BspTNI GGTCTC 1 cut(s) 66
BsrI ACTGG 3 cut(s) 518, 652, 763
BssECI CCNNGG 1 cut(s) 422
BssMI GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
BssNI GRCGYC 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 4 cut(s) 60, 417, 777, 878
BstACI GRCGYC 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 224, 377
BstDEI CTNAG 3 cut(s) 345, 481, 690
BstF5I GGATG 3 cut(s) 228, 592, 643
BstKTI GATC 8 cut(s) 121, 127, 162, 360, 553, 569, 732, 790
BstMAI GTCTC 2 cut(s) 66, 177
BstMBI GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
BstMWI GCNNNNNNNGC 1 cut(s) 112
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 2 cut(s) 333, 614
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 773
BstV1I GCAGC 1 cut(s) 719
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 2 cut(s) 118, 729
BstYI RGATCY 2 cut(s) 118, 729
BtgZI GCGATG 1 cut(s) 445
BtsCI GGATG 3 cut(s) 228, 592, 643
BtsI GCAGTG 4 cut(s) 272, 402, 747, 802
BtsIMutI CAGTG 5 cut(s) 272, 402, 664, 747, 802
Cac8I GCNNGC 2 cut(s) 224, 377
Cfr13I GGNCC 1 cut(s) 595
Csp6I GTAC 4 cut(s) 56, 199, 778, 874
CviAII CATG 6 cut(s) 300, 330, 611, 782, 791, 827
CviQI GTAC 4 cut(s) 56, 199, 778, 874
DdeI CTNAG 3 cut(s) 345, 481, 690
DpnI GATC 8 cut(s) 120, 126, 161, 359, 552, 568, 731, 789
DpnII GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
Eco24I GRGCYC 1 cut(s) 689
Eco31I GGTCTC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 689
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 6 cut(s) 303, 333, 614, 785, 794, 830
FaqI GGGAC 1 cut(s) 377
FatI CATG 6 cut(s) 299, 329, 610, 781, 790, 826
FauI CCCGC 2 cut(s) 326, 480
FbaI TGATCA 2 cut(s) 124, 550
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 1 cut(s) 708
FokI GGATG 3 cut(s) 215, 599, 650
FriOI GRGCYC 1 cut(s) 689
Fsp4HI GCNGC 1 cut(s) 708
FspBI CTAG 2 cut(s) 284, 537
GluI GCNGC 1 cut(s) 708
Hin1I GRCGYC 1 cut(s) 498
Hin1II CATG 6 cut(s) 303, 333, 614, 785, 794, 830
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 5 cut(s) 188, 340, 427, 479, 864
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 3 cut(s) 400, 436, 616
Hpy188I TCNGA 3 cut(s) 129, 182, 574
Hpy188III TCNNGA 7 cut(s) 122, 157, 321, 344, 548, 667, 727
Hpy8I GTNNAC 3 cut(s) 400, 436, 616
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 4 cut(s) 60, 417, 777, 878
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 5 cut(s) 36, 106, 222, 707, 769
HpyF10VI GCNNNNNNNGC 1 cut(s) 112
HpyF3I CTNAG 3 cut(s) 345, 481, 690
HpySE526I ACGT 1 cut(s) 498
Hsp92I GRCGYC 1 cut(s) 498
Hsp92II CATG 6 cut(s) 303, 333, 614, 785, 794, 830
Ksp22I TGATCA 2 cut(s) 124, 550
Kzo9I GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
LmnI GCTCC 1 cut(s) 809
Lsp1109I GCAGC 1 cut(s) 719
MaeI CTAG 2 cut(s) 284, 537
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 108, 380
MalI GATC 8 cut(s) 120, 126, 161, 359, 552, 568, 731, 789
MboI GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
MboII GAAGA 5 cut(s) 142, 309, 436, 534, 797
MflI RGATCY 2 cut(s) 118, 729
MhlI GDGCHC 2 cut(s) 664, 689
MluCI AATT 5 cut(s) 623, 757, 820, 852, 857
MlyI GAGTC 1 cut(s) 473
MnlI CCTC 5 cut(s) 81, 136, 242, 538, 586
MseI TTAA 4 cut(s) 326, 411, 717, 883
MslI CAYNNNNRTG 1 cut(s) 795
MspA1I CMGCKG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 423
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 1 cut(s) 112
NdeII GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
NlaIII CATG 6 cut(s) 303, 333, 614, 785, 794, 830
NlaIV GGNNCC 2 cut(s) 489, 558
NmeAIII GCCGAG 1 cut(s) 43
NspI RCATGY 2 cut(s) 333, 614
PaeR7I CTCGAG 1 cut(s) 68
PciI ACATGT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 4 cut(s) 188, 340, 427, 864
Pfl23II CGTACG 1 cut(s) 198
PkrI GCNGC 1 cut(s) 709
PleI GAGTC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 473
PscI ACATGT 1 cut(s) 610
PshBI ATTAAT 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspLI CGTACG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 489, 558
PspPI GGNCC 1 cut(s) 595
PspXI VCTCGAGB 1 cut(s) 68
PsuI RGATCY 2 cut(s) 118, 729
RsaI GTAC 4 cut(s) 57, 200, 779, 875
RsaNI GTAC 4 cut(s) 56, 199, 778, 874
RseI CAYNNNNRTG 1 cut(s) 795
SalI GTCGAC 1 cut(s) 398
SaqAI TTAA 4 cut(s) 326, 411, 717, 883
SatI GCNGC 1 cut(s) 708
Sau3AI GATC 8 cut(s) 118, 124, 159, 357, 550, 566, 729, 787
Sau96I GGNCC 1 cut(s) 595
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 2 cut(s) 664, 689
SetI ASST 7 cut(s) 92, 228, 285, 289, 351, 501, 597
SfcI CTRYAG 1 cut(s) 773
Sfr274I CTCGAG 1 cut(s) 68
SinI GGWCC 1 cut(s) 595
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 3 cut(s) 68, 665, 725
SmoI CTYRAG 3 cut(s) 68, 665, 725
SpeI ACTAGT 1 cut(s) 536
Sse9I AATT 5 cut(s) 623, 757, 820, 852, 857
SsiI CCGC 3 cut(s) 333, 473, 485
SspI AATATT 1 cut(s) 816
SspMI CTAG 2 cut(s) 284, 537
StyD4I CCNGG 1 cut(s) 421
TaaI ACNGT 4 cut(s) 60, 417, 777, 878
TaiI ACGT 1 cut(s) 501
TaqI TCGA 7 cut(s) 69, 162, 210, 399, 522, 835, 842
TasI AATT 5 cut(s) 623, 757, 820, 852, 857
TatI WGTACW 1 cut(s) 873
TfiI GAWTC 4 cut(s) 188, 340, 427, 864
Tru1I TTAA 4 cut(s) 326, 411, 717, 883
Tru9I TTAA 4 cut(s) 326, 411, 717, 883
TscAI CASTG 5 cut(s) 272, 409, 664, 754, 802
TseI GCWGC 1 cut(s) 707
TspDTI ATGAA 3 cut(s) 288, 798, 815
TspRI CASTG 5 cut(s) 272, 409, 664, 754, 802
VpaK11BI GGWCC 1 cut(s) 595
VspI ATTAAT 1 cut(s) 717
XapI RAATTY 1 cut(s) 857
XceI RCATGY 2 cut(s) 333, 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
XhoI CTCGAG 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 284, 537
ZraI GACGTC 1 cut(s) 499
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.