Rorug03G0212600

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010577
Physical Location & Seq
Reverse (-)
19152711 .. 19153700
990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0212600.1

Sequence Viewer

Length: 690 bp
ATGGCAGAGAAAGAAGAAGCCCACTTAAACGGGGCATACTACGGCCCCTCAGTCCCGGCCAGCAAATCCTACCACCGTCCCAGCCGCGGCGGTGGCCCCCTCGGCTGCTGCTGCAGCTGCATCTTCGGCCTCGTCTTCAAAATCATCTTCGCAGCAGTCGTCTTCATGGGACTAGCCTTCCTCGTCTTCTGGCTCATAGTCCGCCCCACTAAAGTCAAGTTCCACGTCACCGACGCCACGCTCACCCAATTCAACTTCTCCACCGATAGCAACCTGCATTACAACCTAGCCCTCAACCTCACCATTCGAAACCCCAACAAGAAGATCGGTGTCTACTACGATCGCATTGAAGCCAGAGCTCTTTACGAGGGCCAGAGGTTTAGTACGGTCACCCTCACGCCATTTTACCAAGGCCACAAAACTACAAACGTTTTGAACCCGGTGTTTCAGGGACAGCAATTGATTGTTGGATCCAACTTGCTTGAGGAGTTTAATCAACAGAAAAGTGCAGGGGTTTACGAGATTGAGATGAAGCTTTATTTGAGGATTCGGTTCAAGTTGGGTAGAATCAAGACTGGAAAGTTCAAGCCTAGAATTGAATGTGACATGAAGGTTCCTTTGAACCAGAATGGGAATTCAGTCGGTACTTTTCAGACGGAGAAGTGCTCAGTAGATTACTTTAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

229

Amino Acids

25.98

Weight (kDa)

9.4

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 100 - 196 2e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 282
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 87
AciI CCGC 4 cut(s) 85, 87, 90, 202
AclI AACGTT 1 cut(s) 429
AclWI GGATC 2 cut(s) 465, 478
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 634
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 2 cut(s) 385, 646
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 8 cut(s) 139, 253, 350, 436, 556, 586, 599, 622
AjiI CACGTC 1 cut(s) 226
AluBI AGCT 3 cut(s) 117, 359, 535
AluI AGCT 3 cut(s) 117, 359, 535
Alw21I GWGCWC 2 cut(s) 361, 668
AlwI GGATC 2 cut(s) 465, 478
AoxI GGCC 6 cut(s) 43, 57, 94, 127, 370, 412
ApeKI GCWGC 6 cut(s) 105, 108, 111, 114, 117, 152
ApoI RAATTY 1 cut(s) 634
AspS9I GGNCC 3 cut(s) 44, 95, 370
AsuC2I CCSGG 2 cut(s) 56, 440
AsuHPI GGTGA 4 cut(s) 220, 235, 292, 382
AsuII TTCGAA 1 cut(s) 307
BamHI GGATCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 3 cut(s) 127, 154, 178
Bbv12I GWGCWC 2 cut(s) 361, 668
BbvI GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
BceAI ACGGC 1 cut(s) 58
BcnI CCSGG 2 cut(s) 56, 440
BfaI CTAG 3 cut(s) 173, 287, 591
BfmI CTRYAG 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 282
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
BlsI GCNGC 8 cut(s) 86, 89, 107, 110, 113, 116, 119, 154
Bme1390I CCNGG 2 cut(s) 56, 440
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 44, 95, 370
BmiI GGNNCC 4 cut(s) 46, 97, 472, 615
BmrFI CCNGG 2 cut(s) 56, 440
BmsI GCATC 1 cut(s) 129
BpiI GAAGAC 3 cut(s) 127, 154, 178
BplI GAGNNNNNCTC 2 cut(s) 650, 682
Bpu14I TTCGAA 1 cut(s) 307
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 2 cut(s) 56, 440
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 3 cut(s) 85, 100, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 85, 100, 409
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 63, 681
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 6 cut(s) 45, 59, 96, 129, 372, 414
BsiEI CGRYCG 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 361, 668
BsiSI CCGG 2 cut(s) 56, 440
BslFI GGGAC 4 cut(s) 38, 63, 183, 465
BslI CCNNNNNNNGG 1 cut(s) 86
BsmFI GGGAC 4 cut(s) 38, 63, 183, 465
BsnI GGCC 6 cut(s) 45, 59, 96, 129, 372, 414
Bsp119I TTCGAA 1 cut(s) 307
Bsp1286I GDGCHC 2 cut(s) 361, 668
Bsp143I GATC 3 cut(s) 324, 340, 470
BspACI CCGC 4 cut(s) 85, 87, 90, 202
BspANI GGCC 6 cut(s) 45, 59, 96, 129, 372, 414
BspCNI CTCAG 2 cut(s) 62, 680
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 4 cut(s) 46, 97, 472, 615
BspMAI CTGCAG 1 cut(s) 116
BspMI ACCTGC 1 cut(s) 282
BspPI GGATC 2 cut(s) 465, 478
BspT104I TTCGAA 1 cut(s) 307
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 3 cut(s) 85, 100, 409
BssMI GATC 3 cut(s) 324, 340, 470
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 77, 388
BstACI GRCGYC 1 cut(s) 234
BstBI TTCGAA 1 cut(s) 307
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 2 cut(s) 49, 667
BstDSI CCRYGG 1 cut(s) 85
BstEII GGTNACC 1 cut(s) 388
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 327, 343, 473
BstMBI GATC 3 cut(s) 324, 340, 470
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 6 cut(s) 93, 102, 111, 114, 117, 126
BstPI GGTNACC 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 54, 438
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
BstV2I GAAGAC 3 cut(s) 127, 154, 178
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 6 cut(s) 45, 59, 96, 129, 372, 414
BtgI CCRYGG 1 cut(s) 85
BtrI CACGTC 1 cut(s) 226
BveI ACCTGC 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 61
Cfr13I GGNCC 3 cut(s) 44, 95, 370
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 384, 645
CviAII CATG 2 cut(s) 166, 607
CviQI GTAC 2 cut(s) 384, 645
DdeI CTNAG 2 cut(s) 49, 667
DpnI GATC 3 cut(s) 326, 342, 472
DpnII GATC 3 cut(s) 324, 340, 470
EaeI YGGCCR 1 cut(s) 57
EciI GGCGGA 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 2 cut(s) 169, 610
FaiI YATR 4 cut(s) 37, 167, 197, 608
FaqI GGGAC 4 cut(s) 38, 63, 183, 465
FatI CATG 2 cut(s) 165, 606
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
FspBI CTAG 3 cut(s) 173, 287, 591
GluI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
GsaI CCCAGC 1 cut(s) 84
HaeIII GGCC 6 cut(s) 45, 59, 96, 129, 372, 414
HapII CCGG 2 cut(s) 56, 440
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 2 cut(s) 169, 610
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 2 cut(s) 547, 567
HpaII CCGG 2 cut(s) 56, 440
HphI GGTGA 4 cut(s) 220, 235, 292, 382
Hpy166II GTNNAC 2 cut(s) 334, 517
Hpy188I TCNGA 1 cut(s) 654
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 2 cut(s) 334, 517
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 187, 604
HpyCH4III ACNGT 2 cut(s) 77, 388
HpyCH4IV ACGT 2 cut(s) 225, 429
HpyCH4V TGCA 4 cut(s) 114, 120, 277, 509
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 102, 111, 114, 117, 126
HpyF3I CTNAG 2 cut(s) 49, 667
HpySE526I ACGT 2 cut(s) 225, 429
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 2 cut(s) 169, 610
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 3 cut(s) 324, 340, 470
Lsp1109I GCAGC 6 cut(s) 92, 95, 98, 104, 126, 164
LweI GCATC 1 cut(s) 129
MaeI CTAG 3 cut(s) 173, 287, 591
MaeII ACGT 2 cut(s) 225, 429
MaeIII GTNAC 3 cut(s) 226, 388, 602
MalI GATC 3 cut(s) 326, 342, 472
MboI GATC 3 cut(s) 324, 340, 470
MboII GAAGA 7 cut(s) 26, 115, 127, 139, 154, 178, 334
MfeI CAATTG 1 cut(s) 458
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 2 cut(s) 361, 668
MluCI AATT 5 cut(s) 248, 458, 594, 634, 682
MmeI TCCRAC 2 cut(s) 448, 498
MseI TTAA 3 cut(s) 26, 492, 681
MspA1I CMGCKG 2 cut(s) 87, 117
MspI CCGG 2 cut(s) 56, 440
MspR9I CCNGG 2 cut(s) 56, 440
MunI CAATTG 1 cut(s) 458
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 6 cut(s) 93, 102, 111, 114, 117, 126
NciI CCSGG 2 cut(s) 56, 440
NdeII GATC 3 cut(s) 324, 340, 470
NlaIII CATG 2 cut(s) 169, 610
NlaIV GGNNCC 4 cut(s) 46, 97, 472, 615
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 226, 388, 602
NspV TTCGAA 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PfeI GAWTC 2 cut(s) 547, 567
PkrI GCNGC 8 cut(s) 86, 89, 107, 110, 113, 116, 119, 154
Ple19I CGATCG 1 cut(s) 343
Psp124BI GAGCTC 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 429
PspEI GGTNACC 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 80
PspN4I GGNNCC 4 cut(s) 46, 97, 472, 615
PspPI GGNCC 3 cut(s) 44, 95, 370
PstI CTGCAG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 470
PvuI CGATCG 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 2 cut(s) 385, 646
RsaNI GTAC 2 cut(s) 384, 645
SacI GAGCTC 1 cut(s) 361
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 3 cut(s) 26, 492, 681
SatI GCNGC 8 cut(s) 85, 88, 106, 109, 112, 115, 118, 153
Sau3AI GATC 3 cut(s) 324, 340, 470
Sau96I GGNCC 3 cut(s) 44, 95, 370
ScrFI CCNGG 2 cut(s) 56, 440
SduI GDGCHC 2 cut(s) 361, 668
SfaNI GCATC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 307
SgrBI CCGCGG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 5 cut(s) 248, 458, 594, 634, 682
SsiI CCGC 4 cut(s) 85, 87, 90, 202
SspMI CTAG 3 cut(s) 173, 287, 591
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 2 cut(s) 54, 438
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 77, 388
TaiI ACGT 2 cut(s) 228, 432
TaqI TCGA 1 cut(s) 307
TasI AATT 5 cut(s) 248, 458, 594, 634, 682
TauI GCSGC 2 cut(s) 87, 90
TfiI GAWTC 2 cut(s) 547, 567
Tru1I TTAA 3 cut(s) 26, 492, 681
Tru9I TTAA 3 cut(s) 26, 492, 681
TseFI GTSAC 3 cut(s) 226, 388, 602
TseI GCWGC 6 cut(s) 105, 108, 111, 114, 117, 152
Tsp45I GTSAC 3 cut(s) 226, 388, 602
TspDTI ATGAA 3 cut(s) 154, 545, 623
TspGWI ACGGA 1 cut(s) 671
XapI RAATTY 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 3 cut(s) 173, 287, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.